Article
Neurosciences
Lenka Schorova, Nathalie Bedard, Anouar Khayachi, Hung-Hsiang Ho, Joao Bolivar-Pedroso, Julie Huynh, Mikaela Piccirelli, Yifei Wang, Marie Plourde, Wen Luo, Esther del Cid-Pellitero, Irina Shlaifer, Maria Jose Castellanos-Montiel, Ziqi Yu, Dulce Valeria Carrillo Valenzuela, Maria Lacalle-Aurioles, Anita Kriz, Yihong Ye, Thomas M. Durcan, Simon S. Wing
Summary: USP19 deubiquitinase is associated with Parkinson's Disease and promotes secretion of alpha-synuclein through the MAPS pathway. Inactivating USP19 decreases the accumulation of alpha-synuclein aggregates, reduces activation of microglia, and improves behavior in mice. In vitro experiments show that inactivating USP19 also decreases alpha-synuclein accumulation without affecting uptake and propagation of aggregates.
NPJ PARKINSONS DISEASE
(2023)
Article
Multidisciplinary Sciences
Neha Sarodaya, Apoorvi Tyagi, Hyun-Jin Kim, Ju-Seop Kang, Vijai Singh, Seok-Ho Hong, Woo Jin Kim, Kye-Seong Kim, Suresh Ramakrishna
Summary: In this study, we discovered that PKU-linked PAH protein variants are rapidly degraded in cells, but the interacting DUB USP19 can extend their half-lives and increase their abundance through its deubiquitinating activity.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Neha Sarodaya, Apoorvi Tyagi, Hyun-Jin Kim, Jencia Carminha Colaco, Ju-Seop Kang, Woo Jin Kim, Kye-Seong Kim, Suresh Ramakrishna
Summary: This study identified the role of USP19 in regulating PAH protein stability and promoting its metabolic activity.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Oncology
Christian Schwarzenbach, Larissa Tatsch, Juliana Brandstetter Vilar, Birgit Rasenberger, Lea Beltzig, Bernd Kaina, Maja T. Tomicic, Markus Christmann
Summary: Malignant glioma treatment relies on O-6-methylguanine induction by TMZ, but most glioma cells evade apoptosis and become senescent post-TMZ exposure. Inhibiting SCAP factors, c-IAP2 and Bcl-2, enhances TMZ toxicity and reduces senescent cell protection. BV6 and venetoclax act as senolytic agents in glioblastoma cells post-TMZ exposure, increasing cell death and showing synergistic effects.
Article
Biochemistry & Molecular Biology
Zhijun Xu, Hao Hu, Debao Fang, Jiong Wang, Kailiang Zhao
Summary: The research revealed a novel role of ubiquitin-specific protease USP38 in regulating the abundance and stability of c-Myc. USP38 could inhibit the polyubiquitination of c-Myc, leading to increased stability and promotion of cell proliferation. Additionally, USP38 was found to interact with FBW7 alpha to counteract its degradation effect on c-Myc and restore the inhibitory role of FBW7 alpha on cell proliferation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Oncology
Xiaoqiang Zhang, Xuyu Chen, Fangze Qian, Yanhui Zhu, Gao He, Junzhe Yang, Xian Wu, Hongfei Zhang, Xiafei Yu, Xiaoan Liu
Summary: Our study reveals that USP19 elevates the intracellular Ca2+ concentration to alter ER stress via regulation of BAG6 and BCL2 stability and may be a viable therapeutic target for TNBC therapy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Ruohan Zhang, Serra Ozgen, Hongke Luo, Judith Krigman, Yutong Zhao, Gang Xin, Nuo Sun
Summary: In this study, the researchers investigate the role of Parkin and USP30 in regulating the AKT/mTOR signaling during mitophagy. They found that Parkin inhibits AKT/mTOR signaling, while USP30 antagonizes the activity of Parkin to sustain AKT/mTOR activity.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Diana Campos-Iglesias, Julia M. Fraile, Gabriel Bretones, Alejandro A. Montero, Elena Bonzon-Kulichenko, Jesus Vazquez, Carlos Lopez-Otin, Jose M. P. Freije
Summary: USP49 deubiquitinase is identified as a novel regulator of the spindle checkpoint. Loss of USP49 impairs proliferation and increases aneuploidy in cancer cell lines. USP49-depleted cells can overcome SAC-induced arrest in presence of nocodazole.
CELL DEATH & DISEASE
(2023)
Article
Biotechnology & Applied Microbiology
Arun Pandian Chandrasekaran, Apoorvi Tyagi, Naresh Poondla, Neha Sarodaya, Janardhan Keshav Karapurkar, Kamini Kaushal, Chang-Hwan Park, Seok-Ho Hong, Kye-Seong Kim, Suresh Ramakrishna
Summary: This study demonstrates that USP19 plays a dual role in mitosis and tumorigenesis by regulating Survivin expression and protein stability. Depletion of USP19 leads to mitotic defects and inhibits tumor growth.
Article
Biochemistry & Molecular Biology
Ming Gao, Zijuan Qi, Min Deng, Hongyang Huang, Zhijie Xu, Guijie Guo, Jiajun Jing, Xiaofeng Huang, Ming Xu, Jake A. Kloeber, Sijin Liu, Jinzhou Huang, Zhenkun Lou, Jinxiang Han
Summary: The deubiquitinase USP7 plays a key role in controlling redox homeostasis by promoting HO-1 deubiquitination and stabilization in hepatocytes. USP7 inhibitor might be a potential therapeutic agent for treating HO-1 overexpressed liver cancers.
Article
Plant Sciences
Jing Sun, Wenzhong Song, Yuan Chang, Yanwei Wang, Tiegang Lu, Zhiguo Zhang
Summary: This study identified a rice lesion mimic mutant and investigated the regulatory mechanism of the lesion mimic phenotype. The results show that OsLMP1 modifies SA synthetic pathway genes by deubiquitination, thereby regulating the immune response in rice.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Trisha A. Macrae, Miguel Ramalho-Santos
Summary: The study reveals a regulatory axis between the deubiquitinase Usp9x and PRC2 that is critical for self-renewal of mouse embryonic stem cells. Different levels of Usp9x lead to distinct chromatin and transcriptional patterns in ES cells, reminiscent of different developmental stages. Deletion of Usp9x in post-implantation embryos results in derepression of genes and morphological abnormalities, suggesting a crucial role of Usp9x-PRC2 axis in development.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Apoorvi Tyagi, Janardhan Keshav Karapurkar, Jencia Carminha Colaco, Neha Sarodaya, Ainsley Mike Antao, Kamini Kaushal, Saba Haq, Arun Pandian Chandrasekaran, Soumyadip Das, Vijai Singh, Seok-Ho Hong, Bharathi Suresh, Kye-Seong Kim, Suresh Ramakrishna
Summary: In this study, USP19 is identified as a negative regulator of p53 protein level in cervical cancer progression. The direct interaction between USP19 and p53 is demonstrated by pull down assay. Overexpression of USP19 promotes ubiquitination of p53 and reduces its protein half-life. CRISPR/Cas9-mediated knockout of USP19 elevates p53 protein levels, resulting in reduced colony formation, cell migration, and cell invasion in cervical cancer cells.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jaemin Shin, Young Hun Kim, Bin Lee, Jae Ho Chang, Hee Youn Choi, Hoojung Lee, Ki Chan Song, Man Sup Kwak, Ji Eun Choi, Jeon-Soo Shin
Summary: Through various experiments, we found ubiquitin-specific protease 13 (USP13) as a novel binding partner of HMGB1 and demonstrated that USP13 plays a role in stabilizing HMGB1 from ubiquitin-mediated degradation. Further investigation showed that USP13 overexpression increased nucleocytoplasmic translocation of HMGB1 and promoted its secretion, which was inhibited by treatment with Spautin-1.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Alexander Vdovin, Tomas Jelinek, David Zihala, Tereza Sevcikova, Michal Durech, Hana Sahinbegovic, Renata Snaurova, Dhwani Radhakrishnan, Marcello Turi, Zuzana Chyra, Tereza Popkova, Ondrej Venglar, Matous Hrdinka, Roman Hajek, Michal Simicek
Summary: Development of proteasome inhibitor resistance is a common problem in patients with multiple myeloma. The authors show that deubiquitinase OTUD1 protects PRDX4 from degradation, resulting in increased load of misfolded immunoglobulins and sensitizing myeloma cells for proteasome inhibition.
NATURE COMMUNICATIONS
(2022)
Letter
Hematology
Anindita Ghosh, Lei Chen, Amer Wahed, Wei Wang, Karan Saluja, Andy N. D. Nguyen, Shimin Hu, Zhihong Hu
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Letter
Oncology
Siba El Hussein, Shimin Hu, Hong Fang, Sofia Garces, Tariq Muzzafar, Sa A. Wang, L. Jeffrey Medeiros, Carlos Bueso-Ramos, FatimaZahra Jelloul
LEUKEMIA & LYMPHOMA
(2022)
Review
Pathology
Yuhua Huang, Shimin Hu, Daniel P. Larson, Min Shi, Rong He, Bhavana J. Dave, Timothy C. Greiner, Kai Fu, Ellen D. McPhail, Rhett P. Ketterling, L. Jeffrey Medeiros, Ji Yuan
Summary: Composite classic Hodgkin lymphoma and follicular lymphoma (CHLFL) is a rare entity that predominantly occurs in older adults, involves lymph nodes, and most commonly presents as de novo cases. In a small number of cases assessed, the CHL and FL components are usually clonally related.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2022)
Article
Hematology
Guilin Tang, Ying Zou, Sa A. Wang, Gautam Borthakur, Gokce Toruner, Shimin Hu, Shaoying Li, Jie Xu, L. Jeffrey Medeiros, Zhenya Tang
Summary: Fluorescence in situ hybridization (FISH) analysis is widely used for detecting CBFB rearrangement (CBFBr) in acute myeloid leukemia (AML). However, the clinical importance of 3'CBFB deletion (3'CBFBdel) associated with CBFBr is largely unknown. This study identified AML patients with 3'CBFBdel and found that most of them had CBFB::MYH11 fusion. These patients showed similar pathological features to AML with inv(16), but had different mutation profiles and a higher risk of relapse requiring hematopoietic stem cell transplant.
ANNALS OF HEMATOLOGY
(2022)
Article
Cell Biology
Hui Liu, Zijun Y. Xu-Monette, Guilin Tang, Wei Wang, Young Kim, Ji Yuan, Yu Li, Weina Chen, Yanping Li, George Y. Fedoriw, Feng Zhu, Xiaosheng Fang, Catherine Luedke, L. Jeffrey Medeiros, Ken H. Young, Shimin Hu
Summary: This study identified 16 cases of EBV+ DHL/THL, with half presenting high-grade/Burkitt-like morphology and the other half diffuse large B cell lymphoma morphology. Using Hans' algorithm, 13 cases were classified as germinal centre B cell and 3 as non-GCB. Most cases showed EBV latency Type I, with a median of 80% positive cells for EBV-encoded small RNAs.
Review
Pathology
Siba El Hussein, L. Jeffrey Medeiros, Shimin Hu, Pei Lin, Wei Wang
Summary: This review provides a concise yet comprehensive summary of the morphological and immunophenotypical variants of plasma cell neoplasms, emphasizing the potential diagnostic pitfalls.
Letter
Hematology
Xiaoqiong Wang, Guilin Tang, Zhihong Hu, Hong Fang, Wei Wang, Zhenya Tang, Gokce A. Toruner, Ting Zhou, Courtney D. DiNardo, Guillermo Garcia-Manero, Srdan Verstovsek, Carlos E. Bueso-Ramos, L. Jeffrey Medeiros, Shimin Hu
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Pathology
Mehrnoosh Tashakori, Joseph D. Khoury, Mark J. Routbort, Keyur P. Patel, Sa A. Wang, Chi Young Ok, Siba El-Hussein, Rashmi Kanagal-Shamanna, Rajyalakshmi Luthra, Shimin Hu, Pei Lin, Naveen Pemmaraju, Prithviraj Bose, Srdan Verstovsek, Carlos E. Bueso-Ramos, L. Jeffrey Medeiros, Sanam Loghavi
Summary: This study evaluated the clinicopathologic spectrum of myeloid neoplasms with concurrent MPN-driver mutations and SRSF2 mutations. The results suggest that patients with these mutations exhibit clinical and morphologic features similar to classic MPNs, often with dysplasia and monocytosis.
Article
Pathology
Hong Fang, Joseph D. Khoury, Carlos A. Torres-Cabala, Siok Bian Ng, Jie Xu, Siba El Hussein, Shimin Hu, Francisco Vega, Shaoying Li, Zhenya Tang, Guilin Tang, L. Jeffrey Medeiros, Wei Wang
Summary: In this study, the expression of BCL11B in various T-cell neoplasms was investigated through immunohistochemical analysis. The results showed that BCL11B was positively expressed in certain types of T-cell lymphomas, but the expression was weaker in reactive cutaneous conditions. BCL11B was negative in NK-cell neoplasms and B-cell lymphomas.
Article
Oncology
Wei Wang, Jie Xu, Joseph D. Khoury, Naveen Pemmaraju, Hong Fang, Roberto N. Miranda, C. Cameron Yin, Siba El Hussein, Fuli Jia, Zhenya Tang, Shimin Hu, Marina Konopleva, L. Jeffrey Medeiros, Sa A. Wang
Summary: This study investigated the immunophenotypic and molecular profiles of pDC-AML and BPDCN and found that they have different phenotypes and mutation profiles, indicating that they are two distinct entities.
Article
Medical Laboratory Technology
Hong Fang, Sa A. Wang, M. James You, Shimin Hu, Roberto N. Miranda, Zhenya Tang, Pei Lin, Jeffrey L. Jorgensen, Jie Xu, Beenu Thakral, Ellen J. Schlette, Siba El Hussein, Carlos Bueso-Ramos, L. Jeffrey Medeiros, Wei Wang
Summary: The immunophenotype of pure erythroid leukemia (PEL) differs significantly from reactive conditions. PEL is characterized by positive expression of CD36 and CD71, dimmer expression of CD45, variable expression of CD117 and CD235a, decreased or negative expression of CD38, aberrant expression of CD7 and CD13, abnormal expression of CD4 and absence of CD34.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2022)
Letter
Oncology
Wei Wang, Brandon T. Gehris, Brenda Mai, Lei Chen, Shimin Hu, M. James You, Zhihong Hu
LEUKEMIA & LYMPHOMA
(2022)
Letter
Hematology
Qianze Dong, Yang Wang, Yan Xiu, Hany Sakr, Bettina Burnworth, Dongbin Xu, Timothy O'Brien, Juanita Burke, Shimin Hu, Gang Zeng, Chen Zhao
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Oncology
Guilin Tang, Shaoying Li, Gokce A. Toruner, Preetesh Jain, Zhenya Tang, Shimin Hu, Jie Xu, Joanne Cheng, Melissa Robinson, Francisco Vega, L. Jeffrey Medeiros
Summary: FISH analysis using MYC break-apart probes is a commonly used technique to assess for MYC rearrangement. However, atypical signal patterns can occur, such as gain or loss of 5'MYC or 3'MYC. This study evaluated patients with aggressive B-cell lymphomas who exhibited atypical FISH signal patterns and found that 5'MYC gain is associated with MYC expression and a poorer prognosis, while 3'MYC deletions are not associated with MYC expression or a poorer prognosis and may be unrelated to MYC-R.
Article
Oncology
Fang Yu, Hua He, Loretta J. Nastoupil, Zijun Y. Xu-Monette, Ky Pham, Yong Liang, Guang Chen, Nathan H. Fowler, C. Cameron Yin, Dongfeng Tan, Yaling Yang, Shimin Hu, Ken H. Young, Lan Pham, M. James You
Summary: FOXM1 is a transcription factor that plays important roles in cell cycle regulation, cell proliferation, and differentiation. Its overexpression is associated with poor prognosis in diffuse large B cell lymphoma (DLBCL). FOXM1 forms a functional axis with PLK1, which is involved in the regulation of cell cycle and DNA damage/repair pathways.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)