Article
Biochemistry & Molecular Biology
Wei Lee, Hiroshi Iwasaki, Hideo Tsubouchi, Hung-Wen Li
Summary: In meiosis, Dmc1 and Rad51 are responsible for pairing and exchanging strands of homologous chromosomes. The mechanisms by which Swi5-Sfr1 and Hop2-Mnd1 stimulate Dmc1-driven recombination are still unclear. Through smFRET and TPM experiments, it was found that both Hop2-Mnd1 and Swi5-Sfr1 individually enhance Dmc1 filament assembly on ssDNA, and when added together, they provide further stimulation. The binding rate of Dmc1 is enhanced by Hop2-Mnd1 while the dissociation rate is specifically reduced by Swi5-Sfr1 during nucleation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Priyanka Sawant, Stephen Mwaniki, Yurika Fujita, Masaru Ito, Asako Furukohri, Akira Shinohara
Summary: In budding yeast, the meiosis-specific protein Mei5-Sae3 promotes the assembly of Dmc1 filaments by stimulating DNA strand exchanges by Rad51 and Dmc1. The YNEL residues in the Sae3 sequence play a critical role in Dmc1 assembly.
GENES & GENETIC SYSTEMS
(2023)
Article
Cell Biology
Hideo Tsubouchi, Bilge Argunhan, Hiroshi Iwasaki
Summary: Homologous recombination (HR) is a universal phenomenon found from viruses to humans, with essentially the same mechanisms in humans and simple unicellular eukaryotes like yeast. The highly diverged yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe, have been exceptionally useful in understanding eukaryotic HR by providing unique biological insights and complementing each other. This review focuses on comparing the features of HR mechanisms in S. pombe with other model organisms, with emphasis on the biochemical characterization of HR mechanisms revealed using S. pombe proteins.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Kodai Hara, Kazuhisa Kinoshita, Tomoko Migita, Kei Murakami, Kenichiro Shimizu, Kozo Takeuchi, Tatsuya Hirano, Hiroshi Hashimoto
Article
Plant Sciences
Ryo Miyata, Muhamad Sahlan, Yoshinobu Ishikawa, Hiroshi Hashimoto, Sari Honda, Shigenori Kumazawa
JOURNAL OF NATURAL PRODUCTS
(2019)
Article
Biochemistry & Molecular Biology
Kodai Hara, Nao Iida, Ryota Tamafune, Eiji Ohashi, Hitomi Sakurai, Yoshinobu Ishikawa, Asami Hishiki, Hiroshi Hashimoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Asami Hishiki, Mamoru Sato, Hiroshi Hashimoto
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Naoyuki Kuwabara, Rieko Imae, Hiroshi Manya, Tomohiro Tanaka, Mamoru Mizuno, Hiroki Tsumoto, Motoi Kanagawa, Kazuhiro Kobayashi, Tatsushi Toda, Toshiya Senda, Tamao Endo, Ryuichi Kato
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Yuko Takagi, Naoyuki Kuwabara, Truong Tat Dang, Koji Furukawa, C. Kiong Ho
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Hideo Tsubouchi, Bilge Argunhan, Kentaro Ito, Masayuki Takahashi, Hiroshi Iwasaki
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biology
Bilge Argunhan, Masayoshi Sakakura, Negar Afshar, Misato Kurihara, Kentaro Ito, Takahisa Maki, Shuji Kanamaru, Yasuto Murayama, Hideo Tsubouchi, Masayuki Takahashi, Hideo Takahashi, Hiroshi Iwasaki
Article
Biochemical Research Methods
Hideshi Yokoyama, Kana Suzuki, Kodai Hara, Ikuo Matsui, Hiroshi Hashimoto
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Kentaro Ito, Yasuto Murayama, Yumiko Kurokawa, Shuji Kanamaru, Yuichi Kokabu, Takahisa Maki, Tsutomu Mikawa, Bilge Argunhan, Hideo Tsubouchi, Mitsunori Ikeguchi, Masayuki Takahashi, Hiroshi Iwasaki
NATURE COMMUNICATIONS
(2020)
Article
Biochemical Research Methods
Asami Hishiki, Mamoru Sato, Hiroshi Hashimoto
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Biology
Atsushi T. Onaka, Jie Su, Yasuhiro Katahira, Crystal Tang, Faria Zafar, Keita Aoki, Wataru Kagawa, Hironori Niki, Hiroshi Iwasaki, Takuro Nakagawa
COMMUNICATIONS BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Hiroshi Hashimoto, Kodai Hara, Asami Hishiki
Summary: DNA sliding clamps, such as PCNA and 9-1-1, play important roles in DNA replication and repair by recruiting proteins involved in these processes. While PCNA and 9-1-1 have similar structures, the mechanism of interaction between 9-1-1 and its partners is distinct from that of PCNA.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Asami Hishiki, Sumire Okazaki, Kodai Hara, Hiroshi Hashimoto
Summary: The sliding DNA clamp protein plays a crucial role in cell survival and proliferation and is considered a promising target for bacterial infection therapy. The crystal structure of the bacteria Clostridioides difficile's DnaN protein has been determined, revealing similarities to DnaN from other bacteria but with differences in the binding pocket for partner proteins. These findings provide insight into the development of new therapies for C. difficile infection.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Microbiology
Yong-Woon Han, Rei Kajitani, Hiroya Morimoto, Maierdan Palihati, Yumiko Kurokawa, Rie Ryusui, Bilge Argunhan, Hideo Tsubouchi, Fumiyoshi Abe, Susumu Kajiwara, Hiroshi Iwasaki, Takehiko Itoh
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)