Review
Cell Biology
Anthony J. Hayes, James Melrose
Summary: Chondroitin sulfate plays crucial roles in cell regulation and structural support in the central and peripheral nervous systems, controlling tissue function and homeostasis, and playing key roles in tissue recovery following neural injuries.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Renjun Tu, Bo Duan, Xiaoqing Song, Shiyuan Chen, Allison Scott, Kate Hall, Jillian Blanck, Dustin DeGraffenreid, Hua Li, Anoja Perera, Jeff Haug, Ting Xie
Summary: In the Drosophila ovary, inner germarial sheath (IGS) cells form multiple niche compartments that interact with different stages of germline stem cell (GSC) progeny, regulating stem cell self-renewal and stepwise differentiation.
Article
Pharmacology & Pharmacy
Eleonora Bianchi, Angela Faccendini, Elena Del Favero, Caterina Ricci, Laura Caliogna, Barbara Vigani, Francesco Claudio Pavesi, Cesare Perotti, Rui M. A. Domingues, Manuela E. Gomes, Silvia Rossi, Giuseppina Sandri
Summary: This study develops a tubular scaffold based on pullulan and chitosan for enhancing enthesis repair. The scaffold has a gradient of nanofiber arrangement and hydroxyapatite nanoparticles, with one part resembling bone tissue and the other part having aligned fibers. Human adipose stem cells are able to grow and differentiate on the scaffold, expressing tendon-specific ECM production or bone tissue in different regions. Chondroitin sulfate promotes cell adhesion and proliferation on the scaffold surface.
Article
Dentistry, Oral Surgery & Medicine
Ruoman Xu, Ziyu Zhou, Danle Lin, Lingling Yuan, Siyu Wang, Meng Xu, Yanan Chen, Xiaoli Hu
Summary: Chondroitin sulfate (CS) immobilized in collagen I (Col I) hydrogels was found to induce odontogenic differentiation of dental pulp stem cells (DPSCs) and promote high-quality reparative dentin formation. CS improved DPSCs proliferation and enhanced mineralization activities. In vivo experiments demonstrated that CS-0.1 hydrogel induced higher quality reparative dentin formation compared to mineral trioxide aggregate. Therefore, CS-Col I hydrogel has the potential for high-quality reparative dentin formation in direct pulp capping.
JOURNAL OF ENDODONTICS
(2023)
Article
Developmental Biology
Collin Knudsen, Woo Seuk Koh, Tomomi Izumikawa, Eriko Nakato, Takuya Akiyama, Akiko Kinoshita-Toyoda, Greg Haugstad, Guichuan Yu, Hidenao Toyoda, Hiroshi Nakato
Summary: Heparan sulfate (HS) and chondroitin sulfate (CS) are glycosaminoglycans that are found in most animal species. This study focused on the role of CS in development by generating mutants for Chondroitin sulfate synthase (Chsy) in Drosophila. The mutants showed altered stiffness of the basement membrane and muscle dysfunction in the ovary, leading to degradation of the organ structure.
Review
Biology
Yara M. Michelacci, Raquel Y. A. Baccarin, Nubia N. P. Rodrigues
Summary: Chondrocytes are the main cells in articular cartilage and are responsible for synthesizing and maintaining the extracellular matrix. The interaction between chondrocytes and the extracellular matrix is crucial for regulating cell phenotype.
Article
Polymer Science
Gulshakhar Kudaibergen, Madina Zhunussova, Ellina A. Mun, Yerlan Ramankulov, Vyacheslav Ogay
Summary: Cryogels are a unique macroporous material for tissue engineering, and the addition of hyaluronic acid can enhance their physicochemical properties. The study demonstrated that increasing hyaluronic acid content in cryogels resulted in increased porosity and pore size, as well as improved swelling behavior. Cryogels also showed no toxicity to stem cells and promoted their differentiation into chondrogenic lineage.
Article
Chemistry, Applied
Xin Liu, Shuai Liu, Rong Yang, Penghui Wang, Wenjie Zhang, Xiaoyan Tan, Yanhan Ren, Bo Chi
Summary: A gradient hydrogel scaffold was designed based on the structure and composition distribution in biological cartilage tissue, showing excellent physical properties and cell compatibility. The scaffold can mimic the function of natural cartilage, making it a potential option for cartilage tissue engineering.
CARBOHYDRATE POLYMERS
(2021)
Article
Cell & Tissue Engineering
Maria Rosaria Ambrosio, Teresa Migliaccio, Fabiana Napolitano, Sarah Di Somma, Giovanni Maneli, Jussara Amato, Bruno Pagano, Antonio Randazzo, Giuseppe Portella, Pietro Formisano, Anna Maria Malfitano
Summary: In this study, the role of G4 motifs in adipogenic differentiation was investigated using human adipose-derived mesenchymal stem cells (ASCs). The results showed that G4 structures were increased in differentiated adipocytes compared to their precursors, and treatment with the G4 ligand Braco-19 reduced G4 content in the mature adipocytes. Furthermore, Braco-19 caused morphological changes and reduced lipid droplet formation and key gene expression in the mature adipocytes. These findings reveal a new role of G4 motifs in human adipocyte differentiation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Li Yao, Ryan Brice, Teresa Shippy
Summary: Bioscaffold implantation is a promising method for repairing and regenerating injured neural tissue. However, current bioscaffold grafts only result in limited functional recovery. This study explores the use of a neural scaffold made from a combination of collagen and glutenin, testing its effect on astrocyte behavior. The results show that astrocytes grown on glutenin-collagen scaffolds exhibit increased motility and decreased proliferation compared to those grown on collagen scaffolds.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Materials Science, Biomaterials
Jiaqi Hu, Chenlin Li, Zhangze Yang, Qi Wu, Jie Wang, Zongpu Xu, Yuyin Chen, Quan Wan, Yajun Shuai, Shuxu Yang, Mingying Yang
Summary: This study presents a novel fabrication strategy using silk fibroin to develop hierarchical microchannel scaffolds for biomimetic nerve microenvironments. The scaffolds successfully induced cell infiltration and migration, and effectively stimulated neural stem cells to differentiate into neurons. When implanted in vivo, the scaffolds induced a mild inflammatory response, recruited cells extensively, and showed potential for nerve injury repair and regenerative medicine.
BIOMATERIALS SCIENCE
(2023)
Article
Oncology
Saul Perez-Castrillo, Maria Luisa Gonzalez-Fernandez, Jessica Alvarez-Suarez, Jaime Sanchez-Lazaro, Marta Esteban-Blanco, Laura Gutierrez-Velasco, Elsa Gonzalez-Cubero, Vega Villar-Suarez
Summary: Osteoarthritis is a degenerative joint disease affecting the whole joint structure. Studies have shown the effectiveness of combining adipose tissue-derived mesenchymal stem cells and chondroitin sulfate in reducing the expression of inflammatory factors, but not in increasing the expression of cartilage matrix proteins.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Engineering, Environmental
Bohyeon Jeong, Jeong Yeob Baek, Jahong Koo, Subin Park, Young-Kyoung Ryu, Kyoung-Shim Kim, Seungjae Zhang, ChiHye Chung, Rumeysa Dogan, Hyung-Seok Choi, Dahun Um, Tae-Kyung Kim, Wang Sik Lee, Jinyoung Jeong, Won-Ho Shin, Jae-Ran Lee, Nam-Soon Kim, Da Yong Lee
Summary: This study investigates the effects of polystyrene nanoplastic (PSNP) on the development of the central nervous system, and finds that exposure to high concentrations of PSNP may result in neurophysiological and cognitive deficits.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jing Li, Yao Zhang, Zhichao Lou, Mingxi Li, Lin Cui, Zhenrong Yang, Lijuan Zhang, Yu Zhang, Ning Gu, Fang Yang
Summary: Magnetic nanobubbles can modulate the differentiation of neural stem cells through the activation of mechanosensory channels and biochemical signaling pathways, potentially improving their clinical applications.
Article
Biochemistry & Molecular Biology
Egor Dzyubenko, Michael Fleischer, Daniel Manrique-Castano, Mina Borbor, Christoph Kleinschnitz, Andreas Faissner, Dirk M. Hermann
Summary: Our study shows that the brain's ECM maintains E-I balance by supporting inhibitory control through the retention of inhibitory connectivity. Depletion of the ECM in mature neuronal networks leads to a decrease in inhibitory synapse density and individual postsynaptic scaffolds, which is partially compensated by an increase in synaptic strength.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
A. Graeve, I. Ioannidou, J. Reinhard, D. M. Goerl, A. Faissner, L. C. Weiss
Summary: The study reveals that the perception of chemical predator cues by prey species can lead to a cascade of biological reactions, resulting in the development of defensive morphological features, including an increase in central brain volume and the number of inhibitory post-synaptic sites. This indicates a structural rewiring of nerve-cell connections upon predator perception, shedding light on how the nervous system of prey species interprets predator cues and develops optimized defenses.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Elena Schaberg, Ursula Theocharidis, Marcus May, Katrin Lessmann, Timm Schroeder, Andreas Faissner
Summary: Neural stem and progenitor cells (NSPCs) in the developing spinal cord are influenced by extracellular matrix (ECM) molecules, with chondroitin sulfate proteoglycan (CSPG) DSD-1-PG and sulfation playing important roles. Inhibition of sulfation can interfere with the cell cycle progression of spinal cord NSPCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
David Wegrzyn, Josephine Zokol, Andreas Faissner
Summary: Vav proteins are a type of GEFs that catalyze the exchange of GDP with GTP. In the rodent CNS, Vav3 plays a significant role in neuronal development, but its functions in other cell types are less understood.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Lars Roll, Katrin Lessmann, Oliver Bruestle, Andreas Faissner
Summary: During the development of the nervous system, the extracellular matrix (ECM) plays a crucial role in regulating the formation of specialized cell types. This study focuses on the expression patterns of specific carbohydrate structures in human cerebral organoids, providing insights into their potential functions in early central nervous system development.
Article
Biochemistry & Molecular Biology
Elena Schaberg, Magdalena Gotz, Andreas Faissner
Summary: The study reveals that Tnc protein plays a crucial role in the adult neural stem cell niche, affecting the maturation and proliferation of stem cells, as well as the migration capacity of neuroblasts.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
David Wegrzyn, Georg Juckel, Andreas Faissner
Summary: Schizophrenia is a severe neuropsychiatric disease that affects normal life. Hippocampal abnormalities play a crucial role in schizophrenia and can be observed in both early and late stages of the disease. Studying hippocampal abnormalities can provide a better understanding of the onset and outcomes of schizophrenia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Egor Dzyubenko, Daniel Manrique-Castano, Matthias Pillath-Eilers, Paraskevi Vasileiadou, Jacqueline Reinhard, Andreas Faissner, Dirk M. Hermann
Summary: Cellular responses in glia play a key role in regulating brain remodeling post-stroke. Excessive glial reactivity impedes neuroplasticity and hampers neurological recovery. We found that tenascin-C (TnC) plays a central role in restraining astrogliosis and regulating astrocyte-microglial interactions.
Article
Cell Biology
Juliane Bauch, Andreas Faissner
Summary: Oligodendrocytes play an important role in myelination in the central nervous system. The glycoproteins tenascin-C (Tnc) and tenascin-R (Tnr) regulate the differentiation and regeneration of oligodendrocytes, affecting the formation and thickness of myelin sheaths. Results from experiments using Tnc and Tnr knockout mice show increased number of myelin sheaths and thickness in the absence of these proteins.
Article
Cell Biology
Juliane Bauch, Sina Vom Ort, Annika Ulc, Andreas Faissner
Summary: Recent research has demonstrated the inhibitory effects of tenascin-C (Tnc) and tenascin-R (Tnr) on the formation of myelin membranes through an ex vivo approach. In addition, the cuprizone-based acute demyelination model was successfully used to analyze the remyelination behavior in Tnc(-/-) and Tnr(-/-) mice, revealing inhibitory properties of Tnc and Tnr in myelin membrane formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Cornelius Mueller-Buehl, Jacqueline Reinhard, Lars Roll, Verian Bader, Konstanze F. Winklhofer, Andreas Faissner
Summary: This study investigated the relationship between fast-spiking parvalbumin interneurons, perineuronal nets (PNNs), and synaptic distribution in the mature cortical inhibitory circuits. The results suggest that the specialized extracellular matrix structure plays a crucial role in regulating the interaction between these factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ina Schaefer, Juliane Bauch, David Wegrzyn, Lars Roll, Simon van Leeuwen, Annika Jarocki, Andreas Faissner
Summary: This study investigates the impact of the guanine nucleotide exchange factor Vav3 on the migration behavior of oligodendrocyte precursor cells (OPCs). The results show that Vav3 knockout enhances OPCs' migration capacity on the ECM glycoprotein tenascin-C, while not affecting their migration on other ECM molecules. Furthermore, activation of RhoA GTPase signaling compromises migration, while inhibition of RhoA signaling promotes migration behavior.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Polymer Science
Nils Stamm, Kristin Glotzbach, Andreas Faissner, Ralf Weberskirch
Summary: This study fabricated cationic gels with adjustable cationic strength by using defined soluble precursor copolymers and PEGDA3500 as a crosslinker. The results showed that the concentration of cationic groups in the gel had a significant impact on the number and morphology of adhesive cells. Additionally, a synergistic effect of cationic groups and RGD peptide on cell adhesion was observed.
Correction
Neurosciences
Ewa E. Bres, Dina Safina, Julia Muller, Peter Bedner, Honghong Yang, Xavier Helluy, Olena Shchyglo, Stephan Jansen, Melanie D. Mark, Alexander Esser, Christian Steinhauser, Stefan Herlitze, Claus U. Pietrzik, Swetlana Sirko, Denise Manahan-Vaughan, Andreas Faissner
Article
Neurosciences
David Wegrzyn, Nadja Freund, Andreas Faissner, Georg Juckel
Summary: PNNs, specialized ECM structures, can influence synaptic plasticity, with evidence suggesting microglia promote their loss and contribute to neuropsychiatric disorders. Microglial secreted factors strongly impact PNN integrity, synaptic plasticity, and electrophysiological properties of cultured neurons.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
