Article
Public, Environmental & Occupational Health
Yiting Wang, Wencong He, Ping He, Huiwen Zheng, Yanlin Zhao
Summary: This study investigated differences in antimicrobial susceptibility of Mycobacterium abscessus complex (MABC) subsp. massiliense and subsp. abscessus, finding that multilocus variable number tandem repeat (VNTR) typing may provide valuable information for predicting resistance phenotype.
EPIDEMIOLOGY AND INFECTION
(2021)
Article
Microbiology
Ruoyan Ying, Jinghui Yang, Xiaocui Wu, Fangyou Yu, Wei Sha
Summary: Given the increasing prevalence of NTM diseases and their natural resistance to multiple antibiotics, it is necessary to test the susceptibility of different NTM species to drugs from the MYCO test system and new applied drugs. This study analyzed 241 NTM clinical isolates and found that most of the SGM strains were susceptible to certain antibiotics, while the RGM strains were susceptible to different antibiotics. In addition, epidemiological cutoff values were determined for CLO and BDQ. The study concluded that BDQ and CLO show effective activity against different NTM species in vitro, making them potential treatments for NTM diseases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ke-Zhi Chen, Lu-Ling Wang, Jin-Yan Liu, Jun-Tao Zhao, Si-Jia Huang, Ming-Jie Xiang
Summary: This study aimed to determine the function of Cdc50 in Candida glabrata by constructing a Delta cdc50 null mutant and its complemented strain. The results showed that loss of Cdc50 led to difficulties in yeast budding, increased cell wall integrity, enhanced drug susceptibility and weakened virulence. These findings highlight the importance of Cdc50 in C. glabrata and provide potential targets for new drug research.
Article
Biochemical Research Methods
Tingting Yang, Mingyu Gan, Qingyun Liu, Wenying Liang, Qiqin Tang, Geyang Luo, Tianyu Zuo, Yongchao Guo, Chuangyue Hong, Qibing Li, Weiguo Tan, Qian Gao
Summary: The study established a free, function-rich, user-friendly online platform (SAM-TB) for analyzing MTB WGS data, which includes drug-resistance prediction, genetic relationship analysis, and NTM species identification. SAM-TB shows high accuracy in predicting drug-resistance, can analyze the genetic relationships between multiple strains, and detect mixed MTB and NTM samples. The platform is of great significance in guiding treatment and epidemiological investigation.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Microbiology
Skye R. S. Fishbein, Francesca G. Tomasi, Ian D. Wolf, Charles L. Dulberger, Albert Wang, Hasmik Keshishian, Luke Wallace, Steven A. Carr, Thomas R. Ioerger, E. Hesper Rego, Eric J. Rubin
Summary: The mycobacteria's resistance to antibiotic therapy is partly due to their ability to build proteins into a multilayer cell wall, which is crucial for maintaining cell integrity and antibiotic susceptibility. Loss of the LepA gene affects the abundance of membrane proteins, including the outer membrane porin MspA, influencing antibiotic tolerance.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Infectious Diseases
Thi Thuy Do, Jeronimo Rodriguez-Beltran, Esmeralda Cebrian-Sastre, Alexandro Rodriguez-Rojas, Alfredo Castaneda-Garcia, Jesus Blazquez
Summary: In this study, researchers explored a library of Mycobacterium smegmatis mutants to search for new determinants of rifampicin resistance. They found that a transposon insertion in the MSMEG_1945 gene led to rifampicin resistance and collateral susceptibility to other antitubercular drugs. The MSMEG_1945 protein was identified as an ion channel, essential for maintaining cellular ionic balance and membrane potential, thus modulating susceptibility to antimycobacterial agents.
Article
Immunology
Caroline Corbett, Philipp Finger, Marion Heiss-Neumann, Juergen Bohnert, Ines B. Eder, Melanie Eisele, Inna Friesen, Achim J. Kaasch, Jan Kehrmann, Roland Lang, Juergen Roedel, Susann Roessler, Annika Schmidt, Sophie Schneitler, Daniela Schui, Franziska Schuler, Ludwig Sedlacek, Annerose Serr, Ana-Gabriela Sitaru, Joerg Steinmann, Dirk Wagner, Thomas A. Wichelhaus, Sabine Hofmann-Thiel, Harald Hoffmann
Summary: This study analyzed the prevalence and incidence of non-tuberculous mycobacteria (NTM) pulmonary diseases in Germany over a five-year period. The results showed that the prevalence and incidence of NTM remained stable during this time, and there was an increase in the proportion of NTM subjected to drug susceptibility testing.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Neurosciences
Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens, Chia-Chen Liu, Joshua Knight, Tadafumi C. Ikezu, Kurti Aishe, Yiyang Zhu, Axel Meneses, Cassandra L. Rosenberg, Lindsey A. Kuchenbecker, Lucy K. Vanmaele, Fuyao Li, Kai Chen, Francis Shue, Maxwell V. Dacquel, John Fryer, Akhilesh Pandey, Na Zhao, Guojun Bu
Summary: In this study, a Trem2 H157Y knock-in mouse model was generated to investigate the effects of this mutation on TREM2 processing, synaptic function, and AD-related amyloid pathologies. The results showed that Trem2 H157Y increased TREM2 shedding, resulting in elevated soluble TREM2 levels in the brain and serum. Furthermore, Trem2 H157Y accelerated amyloid-beta clearance and reduced amyloid burden in the presence of amyloid pathology. These findings suggest that TREM2 H157Y may increase AD risk through an amyloid-independent pathway.
MOLECULAR NEURODEGENERATION
(2023)
Article
Biochemistry & Molecular Biology
Sebastian Jusuf, Pu-Ting Dong, Jie Hui, Erlinda R. Ulloa, George Y. Liu, Ji-Xin Cheng
Summary: Group B Streptococcus (GBS) is increasingly recognized as a major cause of soft tissue and invasive diseases in the elderly and diabetic populations. Treatment with antibiotics like penicillin is common, but antimicrobial resistance is rising among GBS strains. Research shows that photobleaching may have dual effects on the virulence and antimicrobial susceptibility of GBS, suggesting a novel approach for GBS infection treatment.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daseul Kim, Ki-Young Kim
Summary: The study demonstrated that Adenophora triphylla var. japonica extract inhibited Candida biofilm formation, increased antifungal susceptibility, and reduced infection. This suggests that the extract may be a promising candidate for biofilm-associated fungal infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Infectious Diseases
Christoph Lange, Erik C. Bottger, Emmanuelle Cambau, David E. Griffith, Lorenzo Guglielmetti, Jakko van Ingen, Shandra L. Knight, Theodore K. Marras, Kenneth N. Olivier, Miguel Santin, Jason E. Stout, Enrico Tortoli, Dirk Wagner, Kevin Winthrop, Charles L. Daley
Summary: This study provides guidelines for the treatment of non-tuberculous mycobacterial pulmonary diseases and management guidance for less common species. However, the evidence for treatment recommendations for most species is of very low quality, except for M. malmoense.
LANCET INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Nils Wetzstein, Thomas A. Wichelhaus
Summary: Phenotypic drug susceptibility testing is valuable for guiding therapy in rapid growing mycobacteria, but beta lactams may exhibit insufficient stability during testing, leading to potential inaccuracies.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Medicine, Research & Experimental
Francisco Quinonero, Cristina Mesas, Jose A. Munoz-Gamez, Cristina Jimenez-Luna, Gloria Perazzoli, Jose Prados, Consolacion Melguizo, Raul Ortiz
Summary: Inhibition of PARP1 has been found to enhance the sensitivity of pancreatic cancer cells to gemcitabine, reducing tumor growth and migration, as well as decreasing drug resistance and angiogenesis. This suggests that targeting PARP1 could be a promising strategy for the treatment of pancreatic cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Infectious Diseases
Gabrielle Froberg, Florian P. Maurer, Erja Chryssanthou, Louise Fernstrom, Hanaa Benmansour, Samira Boarbi, Anne Torunn Mengshoel, Peter Michael Keller, Miguel Viveiros, Diana Machado, Margaret M. Fitzgibbon, Simone Mok, Jim Werngren, Daniela Maria Cirillo, Fernando Alcaide, Hanne-Leena Hyyrylainen, Alexandra Aubry, Sonke Andres, Darshaalini Nadarajan, Erik Svensson, John Turnidge, Christian G. Giske, Gunnar Kahlmeter, Emmanuelle Cambau, Jakko van Ingen, Thomas Schon
Summary: The study evaluated the minimum inhibitory concentration (MIC) distributions of non-tuberculous mycobacteria (NTM) to establish antimicrobial susceptibility testing (AST) breakpoints. MIC distributions for drugs against Mycobacterium avium complex (MAC) and Mycobacterium abscessus (MAB) were collected from 12 laboratories. Epidemiological cut-off values (ECOFFs) and tentative ECOFFs (TECOFFs) were determined, highlighting the need for further method refinement in establishing clinical breakpoints for NTM.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
Keiji Fujiwara, Fumiko Uesugi, Koji Furuuchi, Yoshiaki Tanaka, Takashi Yoshiyama, Mikio Saotome, Ken Ohta, Satoshi Mitarai, Kozo Morimoto
Summary: The retrospective analysis of MIC results from isolates of rapidly growing mycobacteria from 92 patients with nontuberculous mycobacterial pulmonary disease revealed that Mma were significantly more susceptible to clarithromycin and azithromycin than Mab. Most isolates were susceptible to amikacin and linezolid, while the susceptibility to imipenem was lower.
MICROBIOLOGY SPECTRUM
(2021)