期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 42, 页码 36652-36666出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M111.285924
关键词
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资金
- National Institutes of Health [GM43601, GM67945]
- Charles A. King Trust
Proteasomes consist of a 19-subunit regulatory particle (RP) and 28-subunit core particle (CP), an alpha(7)alpha(7)alpha(7)alpha(7) structure. The RP recognizes substrates and translocates them into the CP for degradation. At the RP-CP interface, a heterohexameric Rpt ring joins to a heteroheptameric CP alpha ring. Rpt C termini insert individually into the alpha ring pockets to form a salt bridge with a pocket lysine residue. We report that substitutions of alpha pocket lysine residues produce an unexpected block to CP assembly, arising from a late stage defect in beta ring assembly. Substitutions alpha 5(K66A) and alpha 6(K62A) resulted in abundant incorporation of immature CP beta subunits, associated with a complete beta ring, into proteasome holoenzymes. Incorporation of immature CP into the proteasome depended on a proteasome-associated protein, Ecm29. Using ump1 mutants, we identified Ecm29 as a potent negative regulator of RP assembly and confirmed our previous findings that proper RP assembly requires the CP. Ecm29 was enriched on proteasomes of pocket lysine mutants, as well as those of rpt4-Delta 1 and rpt6-Delta 1 mutants, in which the C-terminal residue, thought to contact the pocket lysine, is deleted. In both rpt6-Delta 1 and Delta 6(K62A) proteasomes, Ecm29 suppressed opening of the CP substrate translocation channel, which is gated through interactions between Rpt C termini and the alpha pockets. The ubiquitin ligase Hul5 was recruited to these proteasomes together with Ecm29. Proteasome remodeling through the addition of Ecm29 and Hul5 suggests a new layer of the proteasome stress response and may be a common response to structurally aberrant proteasomes or deficient proteasome function.
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