Review
Biochemistry & Molecular Biology
Michela Cerone, Terry K. Smith
Summary: This review provides a comprehensive overview of the role of fatty acid desaturases in the synthesis of naturally occurring fatty acids, discussing their cellular localization, structure, substrate and product specificity, expression and regulation of desaturase genes. The review also explores the mechanisms of fatty acid desaturation and highlights novel applications in meeting the demand for polyunsaturated fatty acids through bioengineering advances.
Article
Biophysics
Hsin-Yang Chang, Li-Hua Lo, Yu-Hsuan Lan, Mao-Xuan Hong, Yuen Ting Chan, Tzu-Ping Ko, Yu-Ru Huang, Tien-Hsing Cheng, Chih-Chuang Liaw
Summary: In this study, a PLP-dependent enzyme gene was identified in marine Vibrio through bioinformatics analysis, and the recombinant protein was characterized for its structural and biochemical properties, providing insights into its substrate binding and selectivity mechanism.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Ravi R. Sonani, Artur Blat, Grzegorz Dubin
Summary: This study solved the crystal structures of human ACOX1a, revealing its homodimeric form and the binding of two FAD molecules at the active site. It also found that the substrate binding cleft of ACOX1a is wider compared to other fatty acid oxidation enzymes. Furthermore, the study analyzed the impact of mutations causing dysfunctionality of ACOX1a on its structure and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Rayda Ben Ayed, Fabienne Moreau, Hajer Ben Hlima, Ahmed Rebai, Sezai Ercisli, Narendra Kadoo, Mohsen Hanana, Amine Assouguem, Riaz Ullah, Essam A. Ali
Summary: The study identified genetic variations in the FAD2 gene in olive oil, which affect the fatty acid ratio and quality; Molecular modeling and docking experiments were used to explore the structure-function relationship of the FAD2 enzyme; Certain SNPs were found to have an impact on the saturated fatty acid content in olive oil.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biology
Florian Bleffert, Joachim Granzin, Muttalip Caliskan, Stephan N. Schott-Verdugo, Meike Siebers, Bjoern Thiele, Laurence Rahme, Sebastian Felgner, Peter Doermann, Holger Gohlke, Renu Batra-Safferling, Karl-Erich Jaeger, Filip Kovacic
Summary: Cells adapt their membrane composition to changing conditions by regulating GPL synthesis and degradation. This study identified a cytoplasmic membrane-bound phospholipase A(1) (PlaF) from Pseudomonas aeruginosa that might be involved in GPL remodeling. PlaF is an important virulence factor and its structure and activation mechanism were revealed, offering insights for the development of therapeutics targeting PlaF.
Article
Plant Sciences
Maria C. Romero-Puertas, Maria Angeles Pelaez-Vico, Diana M. Pazmino, Maria Rodriguez-Serrano, Laura Terron-Camero, Rocio Bautista, Aurelio Gomez-Cadenas, M. Gonzalo Claros, Jose Leon, Luisa M. Sandalio
Summary: The synthetic auxin 2,4-D triggers plant growth defects such as leaf epinasty and stem curvature, with ACX1 identified as a key source of ROS production and involvement in auxin-related signaling pathways. Different organelles play distinct roles in plant responses to 2,4-D, with ACX1 regulating protein degradation associated with auxin signaling.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Xinlin Hu, Ping Yang, Changdong Chai, Jia Liu, Huanhuan Sun, Yanan Wu, Mingjie Zhang, Min Zhang, Xiaotian Liu, Hongjun Yu
Summary: This study reveals the structures and mechanisms of yeast FKS1 and the echinocandin-resistant mutant FKS1(S643P) using cryo-electron microscopy, advancing the understanding of fungal β-1,3-glucan biosynthesis and providing insights into drug-resistant mutations of FKS1. It establishes a foundation for the development of new antifungal drugs by targeting FKS.
Article
Biology
Zhuangfeng Weng, Jiefu Zheng, Yiyi Zhou, Zuer Lu, Yixi Wu, Dongyi Xu, Huanhuan Li, Huanhuan Liang, Yingfang Liu
Summary: This study reports the structural characterization of the MCM8/9 complex using cryo-electron microscopy. The complex is arranged as a heterohexamer with a central channel for DNA binding. The N-terminal OB domains of MCM8/9 have hairpin structures that unwind duplex DNA. Activation by HROB leads to a change in symmetry and rotational motion of the C-tier ring, which is important for the unwinding ability of MCM8/9.
Article
Chemistry, Multidisciplinary
Kirstin Scherlach, Wolfgang Kuttenlochner, Daniel H. Scharf, Axel A. Brakhage, Christian Hertweck, Michael Groll, Eva M. Huber
Summary: This study reveals the mechanism by which the human pathogenic fungus Aspergillus fumigatus incorporates two sulfur atoms into its virulence factor through a specific GST GliG. The structures of GliG with glutathione and its reaction product shed light on the stepwise processing of Phe and Ser moieties in gliotoxin biosynthesis. Enzymatic C-S bond formation in gliotoxin and related natural compounds is further elucidated through activity assays with mutants.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Agronomy
Piera Iommelli, Federico Infascelli, Nadia Musco, Micaela Grossi, Maria Ferrara, Fiorella Sarubbi, Biagio D'Aniello, Pietro Lombardi, Raffaella Tudisco
Summary: This study compared the effects of two different diets on buffalo meat quality and SCD gene expression in adipose tissue, revealing important differences between buffalo and bovine species, highlighting the need for specific research on buffalo.
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Miaoqing Hu, Fan Yang, Yawen Huang, Xin You, Desheng Liu, Shan Sun, Sen-Fang Sui
Summary: NPC1L1 protein is crucial in intestinal cholesterol absorption, and ezetimibe can inhibit NPC1L1 to reduce cholesterol uptake. Cryo-electron microscopy structures revealed that SSD in NPC1L1 responds to alterations in cholesterol levels by binding different numbers of cholesterol molecules, with ezetimibe binding causing inhibition of NPC1L1 function through deformation of the SSD.
Article
Biochemistry & Molecular Biology
Mohammed Shaaban, Julie A. Clapperton, Shan Ding, Simone Kunzelmann, Mart-Erik Maeots, Sarah L. Maslen, J. Mark Skehel, Radoslav I. Enchev
Summary: Modular SCF ubiquitin E3 ligases play important roles in coordinating multiple cellular pathways. Understanding the structural and molecular mechanisms behind the exchange of substrate-bound SCF is crucial. In this study, cryo-EM visualization of a human CAND1-driven exchange reaction provides high-resolution structural intermediates and insights into the conformational changes and dynamics of CAND1-SCF. Additionally, the role of DCNL1 in the CAND1-SCF complex is discovered. Overall, these findings contribute to a detailed model of CAND-SCF regulation.
Article
Food Science & Technology
Wenqi Shi, Jie Li, Yanfang Chen, Yefu Chen, Xuewu Guo, Dongguang Xiao
Summary: Metabolic engineering strategies were utilized to enhance the production of C6-C10 fatty acid ethyl esters in Saccharomyces cerevisiae, including improving precursor accumulation, increasing alcohol acyltransferase activity, and facilitating ester synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Xiu-Juan Wang, Nan Zhang, Zhao-Jie Teng, Peng Wang, Wei-Peng Zhang, Xiu-Lan Chen, Yu-Zhong Zhang, Yin Chen, Hui-Hui Fu, Chun-Yang Li
Summary: Dimethylsulfide (DMS) and dimethylsulfoxide (DMSO) are important components in the global sulfur cycle, with SAR11 and MRC bacteria playing crucial roles in the oxidation of DMS to DMSO. The Tmm enzyme from these bacteria is proposed to have a catalytic mechanism involving a reductive and oxidative half-reaction, with FAD and NADPH molecules being essential for the process. By understanding this mechanism, researchers gain insight into how marine bacteria convert DMS into DMSO, contributing to a better understanding of the global sulfur cycle.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
L. Walter, P. Shrestha, R. Fry, B. J. Leury, A. Logan
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Biotechnology & Applied Microbiology
Madeline C. Mitchell, Jenifer Pritchard, Shoko Okada, Jing Zhang, Ingrid Venables, Thomas Vanhercke, Jean-Philippe Ral
PLANT BIOTECHNOLOGY JOURNAL
(2020)
Article
Plant Sciences
Xue-Rong Zhou, Sajina Bhandari, Brandon S. Johnson, Hari Kiran Kotapati, Doug K. Allen, Thomas Vanhercke, Philip D. Bates
Article
Plant Sciences
Xiao-yu Xu, Sehrish Akbar, Pushkar Shrestha, Lauren Venugoban, Rosangela Devilla, Dawar Hussain, Jiwon Lee, Melanie Rug, Lijun Tian, Thomas Vanhercke, Surinder P. Singh, Zhongyi Li, Peter J. Sharp, Qing Liu
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Dongdi Li, Adam M. Damry, James R. Petrie, Thomas Vanhercke, Surinder P. Singh, Colin J. Jackson
Article
Plant Sciences
Lihong Gao, Wei Chen, Xiaoyu Xu, Jing Zhang, Tanoj K. Singh, Shiming Liu, Dongmei Zhang, Lijun Tian, Adam White, Pushkar Shrestha, Xue-Rong Zhou, Danny Llewellyn, Allan Green, Surinder P. Singh, Qing Liu
PLANT AND CELL PHYSIOLOGY
(2020)
Article
Plant Sciences
James R. Petrie, Xue-Rong Zhou, Antonio Leonforte, Jason McAllister, Pushkar Shrestha, Yoko Kennedy, Srinivas Belide, Greg Buzza, Nelson Gororo, Wenxiang Gao, Geraldine Lester, Maged P. Mansour, Roger J. Mulder, Qing Liu, Lijun Tian, Claudio Silva, Noel O. Cogan, Peter D. Nichols, Allan G. Green, Robert de Feyter, Malcolm D. Devine, Surinder P. Singh
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Agricultural Engineering
Mohammed A. Fouad M. Gaber, Pablo Juliano, Peter Mansour, Pushkar Shrestha, Cheryl Taylor, Rod Smith, Francisco J. Trujillo
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Editorial Material
Plant Sciences
Xue-Rong Zhou, Ikuo Nishida, Mi Chung Suh, Thomas Vanhercke
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Food Science & Technology
P. J. Larkin, X. R. Zhou, Q. Liu, K. Reynolds, T. Vanhercke, J. P. Ral, Z. Li, X. B. Wu, R. Yu, J. X. Luo, M. Newberry, C. H. Howitt
Summary: The transgenic wheat expressing Wrinkled1, DGAT1, and Oleosin genes showed a significant increase in TAG accumulation in the endosperm. Transcriptional impacts on various metabolic pathways were observed, including sucrose transport, glycolysis, and fatty acid synthesis, indicating complex effects on grain development.
JOURNAL OF CEREAL SCIENCE
(2021)
Correction
Plant Sciences
Xue-Rong Zhou, Ikuo Nishida, Mi Chung Suh, Thomas Vanhercke
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Suyan Yee, Vivien Rolland, Kyle B. Reynolds, Pushkar Shrestha, Lina Ma, Surinder P. Singh, Thomas Vanhercke, James R. Petrie, Anna El Tahchy
Summary: Plant oil production has been increasing with investments in high biomass plants and elevated oil content. The expression of specific genes and lipid droplet proteins can significantly increase leaf oil content.
Article
Agriculture, Multidisciplinary
Michelle L. Colgrave, Sonja Dominik, Aarti B. Tobin, Regine Stockmann, Cedric Simon, Crispin A. Howitt, Damien P. Belobrajdic, Cate Paull, Thomas Vanhercke
Summary: The increasing world population and rising affluence are leading to diversification in protein production, with challenges and opportunities for different sources of protein-rich foods such as animals, plants, and emerging sources like insects and microalgae being discussed in terms of taste, nutrition, and sustainability.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)