Article
Multidisciplinary Sciences
Shirley L. Zhang, Nicholas F. Lahens, Zhifeng Yue, Denice M. Arnold, Peter P. Pakstis, Jessica E. Schwarz, Amita Sehgal
Summary: The blood-brain barrier is critical for neural function, and this study shows that efflux of xenobiotics through the BBB follows a circadian rhythm in mice and human cells. Interestingly, limited circadian regulation of transcription was detected in brain endothelial cells, with no evident oscillations in efflux transporters. The molecular clock drives cycling of intracellular magnesium through transcriptional regulation, contributing to the rhythm in efflux.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Meilian Chen, Ji-Hoon Kim, Yun Kyung Lee, Dong-Hun Lee, Young Keun Jin, Jin Hur
Summary: Arctic subsea permafrost contains more organic carbon than terrestrial permafrost and is undergoing rapid degradation due to climate warming. Research shows that the release of dissolved organic matter from subsea permafrost to overlying water column may trigger significant carbon-climate feedbacks.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Studies
Sanku Dattamudi, Saoli Chanda, Leonard J. Scinto
Summary: The study in Northeast Shark River Slough (NESS) showed higher organic phosphorus content downstream and the highest soil organic matter content and CO2 efflux at midway sites, indicating microbial respiration in NESS is limited by the availability of labile carbon rather than phosphorus.
Article
Multidisciplinary Sciences
Pengyu Chen, Kristof De Schutter, Sonia Serna, Simin Chen, Qun Yang, Niels-Christian Reichardt, Els J. M. Van Damme, Guy Smagghe
Summary: This study demonstrates that non-glycosylated rOrysata produced in E. coli has stronger agglutination activity and induces stronger immune responses in Drosophila melanogaster S2 cells compared to glycosylated Orysata produced in yeast, providing insights into how glycosylation can affect protein function and the selection of proper expression systems for recombinant lectin production.
SCIENTIFIC REPORTS
(2021)
Review
Biotechnology & Applied Microbiology
Mengran Chen, Yamei Leng, Chuan He, Xuefeng Li, Lei Zhao, Ying Qu, Yu Wu
Summary: Red blood cells (RBCs) are abundant cells in the body with unique biological and physical properties. Due to their low immunogenicity, long-circulating cycle, and immune characteristics, RBCs have shown great potential as delivery vehicles. This article compares the physiological and physical characteristics of RBCs, analyzes their advantages in delivery systems, and summarizes their applications in biomedicine.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Microbiology
Xuan Wang-Kan, Giovanny Rodriguez-Blanco, Andrew D. Southam, Catherine L. Winder, Warwick B. Dunn, Alasdair Ivens, Laura J. Piddocka
Summary: Our research has demonstrated that the loss of AcrB function in E. coli and S. Typhimurium leads to different biological effects despite conferring drug resistance to the same antibiotics. Additionally, we identified potential natural substrates of AcrB and discovered metabolite classes implicated in the virulence of S. Typhimurium. This information can aid in the development of new efflux inhibitors and potentiate the activity of antibiotics.
Review
Cell Biology
Ponthip Pratumkaew, Surapol Issaragrisil, Sudjit Luanpitpong
Summary: The breakthrough of human induced pluripotent stem cells (hiPSCs) has revolutionized biomedical and pharmaceutical research, offering vast opportunities for drug discovery and regenerative medicine. hiPSCs have been used to model diseases, screen drugs, and develop novel therapeutic targets. Despite challenges, hiPSCs hold great promise in the field of hematology for disease modeling and cell-based therapy.
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Reiko Watanabe, Tsuyoshi Esaki, Rikiya Ohashi, Masataka Kuroda, Hitoshi Kawashima, Hiroshi Komura, Yayoi Natsume-Kitatani, Kenji Mizuguchi
Summary: A computer model was developed to predict the efflux potential of drugs through brain capillary endothelial cells' P-gp, proposing a novel system for evaluating brain-to-plasma concentration ratios of P-gp substrates. Validation with experimental data improved the accuracy of predicting drug penetration across the blood-brain barrier, solely based on chemical structure information, compared to previous studies.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Waqas-ud-Din Khan, Faiza Sharif, Muhammad Asif Naeem, Muhammad Ansar Farooq, Zafar Siddiq, Muhammad Imran
Summary: Chitosan-based composites were used as adsorbents in metal sorption and wastewater treatment, but their application in agriculture under soil salinity has not been explored. This study aimed to select the best adsorbent material and evaluate different application methods for enhancing maize crop's defensive mechanism, ionic concentration, and nutritional value under salt stress. The results showed that the use of chitosan polymerized silica as a source of silicon enhanced the antioxidant activities and nutritional value of maize, improving its tolerance to salt stress. The findings suggest that chitosan-based composites can be an excellent source of silicon for enhancing maize yield under salt stress conditions.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Microbiology
Ying Li, Xizhen Ge
Summary: In this work, we evaluated the role of berberine (BBR) as an inhibitor of the MFS efflux pump MdfA from E. coli. We demonstrated that low levels of BBR significantly increased intracellular ciprofloxacin concentrations and restored antibiotic susceptibility of the reporter strain. Molecular dynamics simulations revealed the effect of BBR on the conformational transition of MdfA. Our data suggested that driving forces for MdfA's conformational transition were affected by BBR and provided evidence for BBR's extended application as an effective inhibitor of MdfA.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Medicinal
Sandra Kovachka, Giuliano Malloci, Meline Simsir, Paolo Ruggerone, Stephane Azoulay, Isabelle Mus-Veteau
Summary: This review focuses on the biological evaluation and molecular mechanistic insights of inhibitors of the drug efflux activity of the Hedgehog receptor Patched1 (Ptch1). Ptch1 is known to be over-expressed in many types of cancers and plays a role in the resistance to chemotherapy of cancer cells. Inhibition of Ptch1 has been shown to improve the efficacy of chemotherapy without toxicity for healthy cells or potential side effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Yongzheng Zhang, Jesus D. Rosado-Lugo, Pratik Datta, Yangsheng Sun, Yanlu Cao, Anamika Banerjee, Yi Yuan, Ajit K. Parhi
Summary: Efflux pumps in Gram-negative bacteria play a crucial role in intrinsic antimicrobial resistance. This study introduces a novel efflux pump inhibitor, TXA09155, which demonstrates potentiation in combination with multiple antibiotics against Pseudomonas aeruginosa. TXA09155 inhibits efflux and lowers the frequency of resistance, showing promise for further development.
Article
Oncology
Dian-Dian Liu, Wei-Cong Hong, Kun-Yin Qiu, Xin-Yu Li, Yong Liu, Li-Wen Zhu, Wei-Xin Lai, Han- Chen, Hua-Qing Yang, Lu-Hong Xu, Jian-Pei Fang
Summary: CAR-T cell therapy is an effective treatment for relapsed and refractory acute lymphoblastic leukemia (R/R ALL). CAR-T cells derived from healthy donors have better antitumor efficacy than those derived from patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Nara Juliana Santos Araujo, Ana Raquel Pereira da Silva, Maria do Socorro Costa, Camila Aparecida Pereira Silva, Thiago Sampaio de Freitas, Erlanio Oliveira de Sousa, Jose Maria Barbosa Filho, Yedda Maria Lobo Soares de Matos, Henrique Douglas Melo Coutinho, Jacqueline Cosmo Andrade-Pinheiro
Summary: Antimicrobial drugs play a crucial role in controlling bacterial infections, but their excessive use leads to the emergence of antibiotic resistance. Hecogenin acetate, a secondary metabolite found in agave plants, has shown significant antibacterial activity. This study aimed to investigate the antibacterial activity of hecogenin acetate against E. coli, P. aeruginosa, and S. aureus, and to examine its inhibitory effects on NorA and MepA efflux pumps in S. aureus strains. The results showed that hecogenin acetate had minimal inhibitory concentration without significant relevance, and did not interfere with the efflux pump mechanism of the analyzed bacteria.
MICROBIAL PATHOGENESIS
(2023)
Review
Neurosciences
Hudson H. Freeze, Erik A. Eklund, Bobby G. Ng, Marc C. Patterson
ANNUAL REVIEW OF NEUROSCIENCE, VOL 38
(2015)
Article
Medical Laboratory Technology
Wenyue Zhang, Philip M. James, Bobby G. Ng, Xueli Li, Baoyun Xia, Jiang Rong, Ghazia Asif, Kimiyo Raymond, Melanie A. Jones, Madhuri Hegde, Tongzhong Ju, Richard D. Cummings, Katie Clarkson, Tim Wood, Cornelius F. Boerkoel, Hudson H. Freeze, Miao He
CLINICAL CHEMISTRY
(2016)
Article
Genetics & Heredity
Ahmet Okay Caglayan, Sinan Comu, Jacob F. Baranoski, Yesim Parman, Hande Kaymakcalan, Gozde Tugce Akgumus, Caner Caglar, Duygu Dolen, Emine Zeynep Erson-Omay, Akdes Serin Harmanci, Ketu Mishra-Gorur, Hudson H. Freeze, Katsuhito Yasuno, Kaya Bilguvar, Murat Gunel
EUROPEAN JOURNAL OF MEDICAL GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Ping He, Jeff E. Grotzke, Bobby G. Ng, Murat Gunel, Hamed Jafar-Nejad, Peter Cresswell, Gregory M. Enns, Hudson H. Freeze
Editorial Material
Biochemistry & Molecular Biology
Ajit Varki, Richard D. Cummings, Markus Aebi, Nicole H. Packer, Peter H. Seeberger, Jeffrey D. Esko, Pamela Stanley, Gerald Hart, Alan Darvill, Taroh Kinoshita, James J. Prestegard, Ronald L. Schnaar, Hudson H. Freeze, Jamey D. Marth, Carolyn R. Bertozzi, Marilynn E. Etzler, Martin Frank, Johannes F. G. Vliegenthart, Thomas Luetteke, Serge Perez, Evan Bolton, Pauline Rudd, James Paulson, Minoru Kanehisa, Philip Toukach, Kiyoko F. Aoki-Kinoshita, Anne Dell, Hisashi Narimatsu, William York, Naoyuki Taniguchi, Stuart Kornfeld
Article
Biochemistry & Molecular Biology
Bobby G. Ng, Lynne A. Wolfe, Mie Ichikawa, Thomas Markello, Miao He, Cynthia J. Tifft, William A. Gahl, Hudson H. Freeze
HUMAN MOLECULAR GENETICS
(2015)
Article
Genetics & Heredity
Bobby G. Ng, Kimiyo Raymond, Martin Kircher, Kati J. Buckingham, Tim Wood, Jay Shendure, Deborah A. Nickerson, Michael J. Bamshad, Jonathan T. S. Wong, Fabiola Paoli Monteiro, Brett H. Graham, Sheryl Jackson, Rebecca Sparkes, Angela E. Scheuerle, Sara Cathey, Fernando Kok, James B. Gibson, Hudson H. Freeze
Article
Endocrinology & Metabolism
Per Bengtson, Bobby G. Ng, Jaak Jaeken, Gert Matthijs, Hudson H. Freeze, Erik A. Eklund
JOURNAL OF INHERITED METABOLIC DISEASE
(2016)
Article
Biochemistry & Molecular Biology
Eric Lau, Yongmei Feng, Giuseppina Claps, Michiko N. Fukuda, Ally Perlina, Dylan Donn, Lucia Jilaveanu, Harriet Kluger, Hudson H. Freeze, Ze'ev A. Ronai
Article
Biochemistry & Molecular Biology
Eric Lau, Yongmei Feng, Giuseppina Claps, Michiko N. Fukuda, Ally Perlina, Dylan Donn, Lucia Jilaveanu, Harriet Kluger, Hudson H. Freeze, Ze'ev A. Ronai
Article
Genetics & Heredity
Megan S. Kane, Mariska Davids, Christopher Adams, Lynne A. Wolfe, Helen W. Cheung, Andrea Gropman, Yan Huang, Bobby G. Ng, Hudson H. Freeze, David R. Adams, William A. Gahl, Cornelius F. Boerkoel
AMERICAN JOURNAL OF HUMAN GENETICS
(2016)
Article
Genetics & Heredity
Bobby G. Ng, Sergey A. Shiryaev, Daisy Rymen, Erik A. Eklund, Kimiyo Raymond, Martin Kircher, Jose E. Abdenur, Fusun Alehan, Alina T. Midro, Michael J. Bamshad, Rita Barone, Gerard T. Berry, Jane E. Brumbaugh, Kati J. Buckingham, Katie Clarkson, F. Sessions Cole, Shawn O'Connor, Gregory M. Cooper, Rudy Van Coster, Laurie A. Demmer, Luisa Diogo, Alexander J. Fay, Can Ficicioglu, Agata Fiumara, William A. Gahl, Rebecca Ganetzky, Himanshu Goel, Lyndsay A. Harshman, Miao He, Jaak Jaeken, Philip M. James, Daniel Katz, Liesbeth Keldermans, Maria Kibaek, Andrew J. Kornberg, Katherine Lachlan, Christina Lam, Joy Yaplito-Lee, Deborah A. Nickerson, Heidi L. Peters, Valerie Race, Luc Regal, Jeffrey S. Rush, S. Lane Rutledge, Jay Shendure, Erika Souche, Susan E. Sparks, Pamela Trapane, Amarilis Sanchez-Valle, Eric Vilain, Arve Vollo, Charles J. Waechter, Raymond Y. Wang, Lynne A. Wolfe, Derek A. Wong, Tim Wood, Amy C. Yang, Univ Washington, Gert Matthijs, Hudson H. Freeze
Article
Cell Biology
Charles DeRossi, Ana Vacaru, Ruhina Rafiq, Ayca Cinaroglu, Dru Imrie, Shikha Nayar, Anastasia Baryshnikova, Miroslav P. Milev, Daniela Stanga, Dhara Kadakia, Ningguo Gao, Jaime Chu, Hudson H. Freeze, Mark A. Lehrman, Michael Sacher, Kirsten C. Sadler
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Biochemistry & Molecular Biology
Lars Hansen, Allan Lind-Thomsen, Hiren J. Joshi, Nis Borbye Pedersen, Christian Theil Have, Yun Kong, Shengjun Wang, Thomas Sparso, Niels Grarup, Malene Bech Vester-Christensen, Katrine Schjoldager, Hudson H. Freeze, Torben Hansen, Oluf Pedersen, Bernard Henrissat, Ulla Mandel, Henrik Clausen, Hans H. Wandall, Eric P. Bennett
Article
Endocrinology & Metabolism
Bobby G. Ng, Hudson H. Freeze
JOURNAL OF INHERITED METABOLIC DISEASE
(2015)