期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 1, 页码 192-198出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.189290
关键词
-
资金
- Royal Society
- COMPAGNIA di San Paolo (Turin)
- Ministero dell'Istruzione dell'Universita e della Ricerca FIRB [RBAU01TJ93]
- PRIN
- Fondazione Cassa di Risparmio di Perugia
- Medical Research Council [MC_U142684172, MC_U142684175] Funding Source: researchfish
- MRC [MC_U142684172, MC_U142684175] Funding Source: UKRI
The molecular identity of ion channels which confer PCO2/pH sensitivity in the brain is unclear. Heteromeric Kir4.1/Kir5.1 channels are highly sensitive to inhibition by intracellular pH and are widely expressed in several brainstem nuclei involved in cardiorespiratory control, including the locus coeruleus. This has therefore led to a proposed role for these channels in neuronal CO2 chemosensitivity. To examine this, we generated mutant mice lacking the Kir5.1 (Kcnj16) gene. We show that although locus coeruleus neurons from Kcnj16((+/+)) mice rapidly respond to cytoplasmic alkalinization and acidification, those from Kcnj16((-/-)) mice display a dramatically reduced and delayed response. These results identify Kir5.1 as an important determinant of PCO2/pH sensitivity in locus coeruleus neurons and suggest that Kir5.1 may be involved in the response to hypercapnic acidosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据