Review
Virology
Giovanna Lucrecia Gallo, Nora Lopez, Maria Eugenia Loureiro
Summary: This study summarizes the research on host molecular determinants during JUNV infection, including viral entry, replication, assembly, and budding, as well as the interaction between JUNV and the innate immune system. Elucidating the interactions between the virus and host cell machinery is crucial for a better understanding of virus replication, disease pathogenesis, and viral suppression of the immune response.
Article
Microbiology
You Zhang, Juan Carlos de la Torre, Gregory B. Melikyan
Summary: This study investigates the role of human LAMP1 in LASV fusion and reveals that ectopic expression of hLAMP1 accelerates the kinetics of small fusion pore formation but only modestly increases LASV fusion and infection. Furthermore, it is found that hLAMP1 is involved in the dilation of LASV fusion pores in the late stage of fusion. These findings shed light on the previously unappreciated role of hLAMP1 in promoting dilation of LASV fusion pores.
Review
Infectious Diseases
Maria G. Frank, Adam Beitscher, Camille M. Webb, Vanessa Raabe
Summary: This article provides a clinical perspective on South American Hemorrhagic Fevers (SAHF), focusing on pathogenesis, clinical features, diagnostics, as well as potential therapies and vaccines. The research identified several treatments and a vaccine platform with potential benefits for treating or preventing SAHF in humans, offering valuable information for clinicians facing patients with the disease.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Larry Zeitlin, Robert W. Cross, Joan B. Geisbert, Viktoriya Borisevich, Krystle N. Agans, Abhishek N. Prasad, Sven Enterlein, M. Javad Aman, Zachary A. Bornholdt, Miles B. Brennan, Lioudmila Campbell, Do Kim, Neil Mlakar, Crystal L. Moyer, Michael H. Pauly, William Shestowsky, Kevin J. Whaley, Karla A. Fenton, Thomas W. Geisbert
Summary: The COVID-19 pandemic has highlighted the importance of finding safe and scalable therapeutic solutions for newly emerging viral pathogens. Monoclonal antibodies have shown success in therapy for viral infections, offering a potential solution for emerging biothreats. For diseases like Argentine hemorrhagic fever, monoclonal antibodies may provide a safer, scalable, and more consistent alternative to immune plasma.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Jennifer Mayor, Giulia Torriani, Olivier Engler, Sylvia Rothenberger
Summary: Hemorrhagic fever viruses, including orthohantaviruses, arenaviruses, and filoviruses, cause severe human diseases and pose a significant challenge to public health. With limited therapeutic options and available vaccines, there is an urgent need for the development of novel antiviral agents. Inhibiting viral attachment and entry shows promise as a strategy for developing new treatments and preventing all subsequent steps in virus infection.
Article
Chemistry, Medicinal
Michael B. Plewe, Vidyasagar Reddy Gantla, Nadezda Sokolova, Young-Jun Shin, Shibani Naik, Eric R. Brown, Alexandra Fetsko, Lihong Zhang, Birte Kalveram, Alexander N. Freiberg, Greg Henkel, Ken McCormack
Summary: The study identified a novel heterocyclic chemical series with potent activity against both Old and New World arenaviruses, showing attractive metabolic stability and lack of hERG K+ channel or CYP enzyme inhibition. Optimized lead compounds could provide a cost-effective broad-spectrum arenavirus therapeutic to help minimize treatment costs for emerging viruses in economically challenged geographical settings.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Virology
Miguel Angel Pelaez, Maria Florencia Torti, Aaron Ezequiel Alvarez De lauro, Agostina Belen Marquez, Federico Giovannoni, Elsa Beatriz Damonte, Cybele Carina Garcia
Summary: This study analyzed the effect of pharmacological modulation of aryl hydrocarbon receptor (AHR) on Junin virus (JUNV) infection, and found that the activation or depression of AHR signaling pathway may play a role in the outcome of human viral infections. Treatment with CH223191, a specific AHR antagonist, significantly inhibited JUNV infection in vitro. These findings suggest the potential of AHR as a therapeutic target for controlling viral infections.
Article
Immunology
Gleyder Roman-Sosa, Anne Leske, Xenia Ficht, Tung Huy Dau, Julia Holzerland, Thomas Hoenen, Martin Beer, Robert Kammerer, Reinhold Schirmbeck, Felix A. Rey, Sandra M. Cordo, Allison Groseth
Summary: In this study, the researchers purified recombinant JUNV GP1 from transfected mammalian cells and induced high-titer virus-neutralizing antibodies in rabbits. These findings highlight the potential of GP1 as a potent inducer of neutralizing antibodies, and emphasize the importance of epitope presentation. The effective virus neutralization by rabbit antibodies also supports the potential use of this species in the development of immunotherapeutics.
Article
Virology
Aaron Ezequiel Alvarez De Lauro, Miguel Angel Pelaez, Agostina Belen Marquez, Mariel Selene Wagner, Luis Alberto Scolaro, Cybele Carina Garcia, Elsa Beatriz Damonte, Claudia Soledad Sepulveda
Summary: This study investigated the effects of the natural flavonoid quercetin (QUER) on the infection of A549 and Vero cells with Junin virus (JUNV). It was found that QUER treatment at non-cytotoxic concentrations prior to infection effectively reduced JUNV multiplication, mainly by affecting the early steps of virus adsorption and internalization. QUER treatment also blocked the phosphorylation of Akt and induced redistribution of the cell receptor TfR1, preventing JUNV entry into the cell.
Article
Immunology
Teresa E. Sorvillo, Robert W. Cross, Dylan M. Johnson, Natalie S. Dobias, Karla A. Fenton, Chad E. Mire, Thomas W. Geisbert
Summary: The rVSV Delta G-JUNVGP vaccine showed excellent protective efficacy against JUNV virus in guinea pig models, with a 100% survival rate in vaccinated animals and robust antibody responses. This study represents the first demonstration of the vaccine's efficacy in providing full protection against lethal JUNV challenge with a single injection.
Article
Cell Biology
Jan Hellert, Andrea Aebischer, Ahmed Haouz, Pablo Guardado-Calvo, Sven Reiche, Martin Beer, Felix A. Rey
Summary: La Crosse virus and Schmallenberg virus, two members of the Orthobunyavirus genus, cause diseases in humans and animals respectively. The study provides the structure of their fusion protein GcF, which is essential for membrane fusion and may aid in the development of therapeutic treatments and vaccines.
Article
Multidisciplinary Sciences
Kathleen A. Cashman, Eric R. Wilkinson, Jeffrey Posakony, Ikenna G. Madu, Eric J. Tarcha, Kurt H. Lustig, Marcus J. Korth, Kristin M. Bedard, Sean M. Amberg
Summary: LHF-535, a small molecule antiviral, shows promising results in reducing viremia and clinical signs in a guinea pig model of Lassa fever, and protects animals from death. Further development of LHF-535 as a therapeutic for Lassa fever is supported by its pharmacokinetics.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Takaaki Koma, Cheng Huang, Adrian Coscia, Steven Hallam, John T. Manning, Junki Maruyama, Aida G. Walker, Milagros Miller, Jeanon N. Smith, Michael Patterson, Jonathan Abraham, Slobodan Paessler
Summary: This study revealed the critical role of specific glycans on GPC in arenavirus pathogenicity, providing insights for rational design of vaccines against hemorrhagic fever-causing viruses in this group.
Article
Virology
Pablo Guardado-Calvo, Felix A. Rey
Summary: Hantaviruses are globally distributed rodent-borne viruses that can be transmitted through the air and have the ability to spread from person to person. While they maintain a non-symptomatic persistent infection in rodent hosts, their spillover to humans can lead to renal or pulmonary syndrome with high fatality rates. The structure of hantavirus particles, including a characteristic surface lattice built up of glycoproteins Gn and Gc, has been studied using X-ray crystallography and cryo-electron tomography, revealing evolutionary relations with alphaviruses.
CURRENT OPINION IN VIROLOGY
(2021)
Article
Microbiology
Takeshi Saito, Takanari Hattori, Kosuke Okuya, Rashid Manzoor, Hiroko Miyamoto, Masahiro Kajihara, Ayato Takada
Summary: Like other arenaviruses, Lujo virus (LUJV) causes viral hemorrhagic fever in humans. The host range and molecular determinants of LUJV susceptibility are not well understood. This study found that rodent-derived cell lines were less susceptible to LUJV infection compared to human-derived cell lines, and the difference was due to the presence of CD63, the cellular receptor for LUJV. Furthermore, a specific amino acid residue on human CD63 was identified as important for LUJV infection. These findings provide insights into the molecular mechanisms underlying LUJV infection and host range restriction, and have implications for the development of antiviral drugs and identification of natural hosts of LUJV.
Article
Chemistry, Medicinal
Gopal R. Bommineni, Kanishk Kapilashrami, Jason E. Cummings, Yang Lu, Susan E. Knudson, Chendi Gu, Stephen G. Walker, Richard A. Slayden, Peter J. Tonge
JOURNAL OF MEDICINAL CHEMISTRY
(2016)
Article
Chemistry, Organic
Sandeep Thanna, Susan E. Knudson, Anna Grzegorzewicz, Sunayana Kapil, Christopher M. Goins, Donald R. Ronning, Mary Jackson, Richard A. Slayden, Steven J. Sucheck
ORGANIC & BIOMOLECULAR CHEMISTRY
(2016)
Article
Orthopedics
D. D. Hemphill, C. W. McIlwraith, R. A. Slayden, R. J. Samulski, L. R. Goodrich
OSTEOARTHRITIS AND CARTILAGE
(2016)
Article
Immunology
Susan E. Knudson, Jason E. Cummings, Gopal R. Bommineni, Pan Pan, Peter J. Tonge, Richard A. Slayden
Article
Chemistry, Organic
Sandeep Thanna, Christopher M. Goins, Susan E. Knudson, Richard A. Slayden, Donald R. Ronning, Steven J. Sucheck
JOURNAL OF ORGANIC CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Lauren A. Spagnuolo, Sandra Eltschkner, Weixuan Yu, Fereidoon Daryaee, Shabnam Davoodi, Susan E. Knudson, Eleanor K. H. Allen, Jonathan Merino, Annica Pschibul, Ben Moree, Neil Thivalapill, James J. Truglio, Joshua Salafsky, Richard A. Slayden, Caroline Kisker, Peter J. Tonge
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Infectious Diseases
Jason E. Cummings, Richard A. Slayden
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Review
Orthopedics
C. Wayne McIlwraith, Christopher E. Kawcak, David D. Frisbie, Christopher B. Little, Peter D. Clegg, Mandy J. Peffers, Morten A. Karsdal, Stina Ekman, Sheila Laverty, Richard A. Slayden, Linda J. Sandell, L. S. Lohmander, Virginia B. Kraus
JOURNAL OF ORTHOPAEDIC RESEARCH
(2018)
Review
Immunology
Richard A. Slayden, Clinton C. Dawson, Jason E. Cummings
PATHOGENS AND DISEASE
(2018)
Article
Pharmacology & Pharmacy
Katriana A. Popichak, Sean L. Hammond, Julie A. Moreno, Maryam F. Afzali, Donald S. Backos, Richard D. Slayden, Stephen Safe, Ronald B. Tjalkens
MOLECULAR PHARMACOLOGY
(2018)
Article
Multidisciplinary Sciences
Collin M. Bantle, C. Tenley French, Jason E. Cummings, Shankar Sadasivan, Kevin Tran, Richard A. Slayden, Richard J. Smeyne, Ronald B. Tjalkens
Summary: Exposure to elevated levels of manganese during juvenile development could sensitize glial cells to more severe neuro-immune responses to influenza infection later in life through persistent epigenetic changes.
Article
Infectious Diseases
Jason E. Cummings, Keaton W. Slayden, Richard A. Slayden
Summary: The study evaluated the antibacterial potency of a novel rifampicin derivative, TPR1, against Francisella tularensis, demonstrating efficacy when delivered alone or in combination with doxycycline. The use of rifampicin derivatives as a platform for the development of novel treatments to other bacterial agents in addition to tularaemia was supported by this work.
JAC-ANTIMICROBIAL RESISTANCE
(2021)
Article
Biochemistry & Molecular Biology
Krupanandan Haranahalli, Simon Tong, Saerom Kim, Monaf Awwa, Lei Chen, Susan E. Knudson, Richard A. Slayden, Eric Singleton, Riccardo Russo, Nancy Connell, Iwao Ojima
Summary: A new library of compounds targeting Mtb-FtsZ was designed, synthesized, and evaluated, with compound 20g showing the highest potency against Mtb-H37Rv. The SAR study led to the discovery of remarkably potent compounds, with 20g predicted as the most effective in the library by the 3DQSAR model.
RSC MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Carla Neckles, Sandra Eltschkner, Jason E. Cummings, Maria Hirschbeck, Fereidoon Daryaee, Gopal R. Bommineni, Zhuo Zhang, Lauren Spagnuolo, Weixuan Yu, Shabnam Davoodi, Richard A. Slayden, Caroline Kisker, Peter J. Tonge
Meeting Abstract
Chemistry, Multidisciplinary
Sandeep Thanna, Susan Knudson, Christopher Goins, Fatma Salem, Sunayana Kapil, Anna Grzegorzewicz, Mary Jackson, Donald Ronning, Richard Slayden, Steven Sucheck
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)