Review
Veterinary Sciences
Tong Zhou, Mingshu Wang, Anchun Cheng, Qiao Yang, Bin Tian, Ying Wu, Renyong Jia, Shun Chen, Mafeng Liu, Xin-Xin Zhao, Xuming Ou, Sai Mao, Di Sun, Shaqiu Zhang, Dekang Zhu, Juan Huang, Qun Gao, Yanling Yu, Ling Zhang
Summary: This article mainly describes the viral protein kinases and their mechanisms of regulating viral protein function through phosphorylation. The study of post-translational modification of viral proteins is of great significance for understanding viral infection mechanisms and developing antiviral treatment.
VETERINARY RESEARCH
(2022)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Qingsong Lu, Xiaozhen Zhang, Tingbo Liang, Xueli Bai
Summary: O-GlcNAc is an important post-translational modification in animals and plants, and its aberrant regulation is associated with various human diseases, including cancer. This review provides an overview of the process of O-GlcNAcylation, the structure and function of O-GlcNAc cycling enzymes, and the involvement of O-GlcNAc in cancer occurrence and progression. It also discusses the potential of O-GlcNAc as a biomarker and therapeutic target for cancer diagnosis, treatment, and prognosis.
MOLECULAR MEDICINE
(2022)
Review
Cell Biology
Xingde Wang, Xingzhi Xu, Zhifeng Wang
Summary: In this review, the authors summarize the known information about the UFM1 enzymatic cascade and its biological functions, discuss the identified substrates, and explore the pathological role of UFMylation in human disease.
Article
Chemistry, Organic
Shu-Xi Jing, Daneel Ferreira, Pankaj Pandey, Larry L. Klein, Amar Gopal Chittiboyina, James B. Mcalpine, David C. Lankin, Yvette Alania, Mariana Reis-Havlat, Ana K. Bedran-Russo, Shao-Nong Chen, Guido F. Pauli
Summary: This study discovered novel trimeric and tetrameric proanthocyanidins (PACs) with unprecedented benzoquinone motifs. Comprehensive spectroscopic analysis, including NMR and ECD, provided vital stereochemical information. This is the first indication that PACs may achieve structural diversity through oxidative oligomerization and post-oligomerizational modifications.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Buyan Pan, Marie Shimogawa, Jun Zhao, Elizabeth Rhoades, Anna Kashina, E. James Petersson
Summary: In this study, a facile method for introducing a mimic of arginylation modification into a protein of interest is presented. The effects of this mimicking modification on protein aggregation and neuroprotection are investigated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Li Cao, Moshe Beiser, Joseph D. Koos, Margarita Orlova, Hader E. Elashal, Hendrik V. Schroder, A. James Link
Summary: Lasso peptides are a family of ribosomally synthesized and post-translationally modified peptides with a threaded structure. PIMT enzymes play a new role in the post-translational modification of lasso peptides, methylating and converting L-aspartate residues to provide rigidity to the lasso peptide ring.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Karlie R. Platz, Emma J. Rudisel, Katelynn V. Paluch, Taylor R. Laurin, Kristin E. Dittenhafer-Reed
Summary: The mitochondrial proteome undergoes various post-translational modifications, and this study focused on the effects of acetylation and phosphorylation on the function of mitochondrial RNA polymerase (POLRMT). While some modifications showed slight decrease in the binding ability of POLRMT, there were minimal biological impacts observed in terms of viability, mtDNA content, and mitochondrial transcript levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Mingyue Zheng, Guoxiang Jin, Zhongjun Zhou
Summary: Lamins, ancient intermediate filament proteins, are functionally regulated by post-translational modifications, contributing to various biological functions. Deregulation of lamins is associated with abnormal nuclear morphology and chromatin disorganization, leading to diseases. Understanding these modifications provides new insights into the molecular mechanisms underlying diseases and potential therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemical Research Methods
Sajad Rashidi, Renu Tuteja, Reza Mansouri, Mohammad Ali-Hassanzadeh, Reza Shafiei, Esmaeel Ghani, Mohammadreza Karimazar, Paul Nguewa, Raul Manzano-Roman
Summary: Investigating post-translational modifications in parasites is crucial for understanding their regulatory roles in survival, infectivity, and pathogenicity, with different forms of PTMs potentially playing key roles in various aspects of parasite biology.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemical Research Methods
Dimitrios Tsikas
Summary: Lysine residues in proteins undergo various chemical modifications, including carbonylation by glyoxal (GO) and methylglyoxal (MGO). Malondialdehyde (MDA) is another carbonyl species formed enzymatically and nonenzymatically. These carbonyl species can occur in free forms or be adducted to proteins, particularly lysine residues. MDA is commonly used as a biomarker of lipid peroxidation, with plasma and serum being the most frequently analyzed samples. However, preanalytical factors, such as artificial MDA formation in lipid-rich samples, can greatly affect MDA concentrations.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dan Wu, Tao Liu
Summary: Understanding post-translational modifications is crucial for manipulating physiological processes in eukaryotes. Genetic code expansion technology has been used to investigate the roles of these modifications, and can be combined with synthetic biology to create genetically modified organisms. This article discusses the applications, limitations, and future perspectives of genetic code expansion technology for studying post-translational modifications, as well as the implications for genetically modified organisms.
Article
Biochemistry & Molecular Biology
Sharon L. Campbell, Mark R. Philips
Summary: Mutations in RAS genes are the most common driving force behind cancer development. RAS proteins, acting as binary molecular switches, control cellular growth through a complex signaling pathway. In addition to nucleotide-binding properties, RAS proteins are also regulated by numerous post-translational modifications, which are currently a high priority for drug discovery research in the field of RAS biology.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Cell Biology
Jing-Hua Yang, Han-Pil Choi, Annie Yang, Roya Azad, Fengmei Chen, Zhangsuo Liu, Kazem M. Azadzoi
Summary: A comparison of protein sequence variations between ischemic and normal bladder tissues revealed a large number of non-coded amino acid residues in bladder ischemia, potentially serving as a novel biomarker for smooth muscle dysfunction.
Review
Oncology
Ana Gonzalez-Garcia, Antonio Garrido, Ana C. Carrera
Summary: Genetic alterations in the PI3-kinase/PTEN pathway are common in cancer cells. Current efforts to treat PTEN-dependent tumors mainly focus on PI3-kinase inhibition, but modulating PTEN post-translational modifications could provide alternative therapeutic strategies.
Article
Biochemistry & Molecular Biology
Christiane Dietrich, Ines Li de la Sierra-Gallay, Muriel Masi, Eric Girard, Nathalie Dautin, Florence Constantinesco-Becker, Maryelle Tropis, Mamadou Daffe, Herman van Tilbeurgh, Nicolas Bayan
MOLECULAR MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Clement Berny, Raphael Le Fevre, Francois Guyot, Karine Blondeau, Christine Guizonne, Emilie Rousseau, Nicolas Bayan, Edouard Alphandery
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Microbiology
Edward T. Lally, Kathleen Boesze-Battaglia, Anuradha Dhingra, Nestor M. Gomez, Jinery Lora, Claire H. Mitchell, Alexander Giannakakis, Syed A. Fahim, Roland Benz, Nataliya Balashova
Article
Microbiology
Nathalie Dautin, Manuela Argentini, Niloofar Mohiman, Cecile Labarre, David Cornu, Laila Sago, Mohamed Chami, Christiane Dietrich, Celia de Sousa D'Auria, Christine Houssin, Muriel Masi, Christophe Salmeron, Nicolas Bayan
Article
Biochemistry & Molecular Biology
Claudio Piselli, Roland Benz
Summary: Pseudomonas aeruginosa, a Gram-negative bacterium, is an opportunistic pathogen responsible for hospital-acquired infections. The bacterium is resistant to antibiotics due to its fine-tuned outer membrane permeability and has substrate-specific porins that play a crucial role in the uptake of antibiotics such as fosmidomycin. The interaction between the porins OprP and OprO with fosmidomycin suggests that these outer membrane channels are essential for fosmidomycin transport across the outer membrane of P. aeruginosa.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Cecile Labarre, Nathalie Dautin, Anna Grzegorzewicz, Mary Jackson, Michael McNeil, Niloofar Mohiman, Laila Sago, Nicolas Bayan
Summary: LppX is crucial for the surface localization of phthiocerol dimycocerosates (DIM) in Mycobacterium tuberculosis, with its glycosylation depending on the mannosyl transferase PMT. However, the absence of glycosylation at residues S16 and T18 does not alter DIM localization in M. tuberculosis.
RESEARCH IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Katharina Ernst, Marc Landenberger, Julian Nieland, Katharina Norgaard, Manfred Frick, Giorgio Fois, Roland Benz, Holger Barth
Summary: The study investigated the cytotoxic effect of CDTb on cells, revealing that CDTb impairs cell viability and leads to redistribution of F-actin without affecting tubulin structures in CaCo-2 cells. Pharmacological pore blockers were found to effectively inhibit the cytotoxicity induced by pore formation of CDTb in the cytoplasmic membrane.
Article
Biophysics
Roland Benz, Claudio Piselli, Cezarela Hoxha, Cornelia Koy, Michael O. Glocker, Michel R. Popoff
Summary: Clostridium perfringens produces a variety of toxins, with one virulent toxin cCPB2 forming highly active cation-selective channels, leading to necrotizing enterocolitis. Further research is exploring the biological activity and phylogenetic relationship of cCPB2 and other toxins.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Article
Chemistry, Physical
Vinaya Kumar Golla, Claudio Piselli, Ulrich Kleinekathoefer, Roland Benz
Summary: This study investigates the permeation of fosfomycin through OprP and OprO channels in Pseudomonas aeruginosa bacteria using electrophysiology and molecular dynamics simulations. The results show that fosfomycin has higher binding affinity to OprP and OprO compared to fosmidomycin, and that arginine residues and lysine residue clusters play an important role in fosfomycin permeation. This study provides molecular insights on improving antibiotic permeation into Gram-negative bacteria, particularly resistant P. aeruginosa strains.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Multidisciplinary
Eszter E. Najbauer, Kumar Tekwani Movellan, Karin Giller, Roland Benz, Stefan Becker, Christian Griesinger, Loren B. Andreas
Summary: In this study, the structure of VDAC1 was successfully determined in a near-native lipid bilayer environment using proton-detected fast magic-angle spinning NMR spectroscopy. Dynamics were found to play a crucial role in the gating behavior of the protein, and cholesterol was shown to stabilize the channel structure.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Organic
Mathieu Carlier, Emilie Lesur, Aurelie Baron, Aurelie Lemetais, Karine Guitot, Loic Roupnel, Christiane Dietrich, Gilles Doisneau, Dominique Urban, Nicolas Bayan, Jean-Marie Beau, Dominique Guianvarc'h, Boris Vauzeilles, Yann Bourdreux
Summary: Trehalose-based probes are useful tools for detecting the mycomembrane of mycobacteria through metabolic labeling. This study simplified the synthesis of trehalose analogues and demonstrated their application in one-step and two-step labeling methods for studying the mycomembrane in live mycobacteria.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Biophysics
Claudio Piselli, Lorraine Benier, Cornelia Koy, Michael O. Glocker, Roland Benz
Summary: The cell wall of Rhodococcus corynebacteroides and Rhodococcus ruber contains cell wall channels formed by polypeptide subunits, which may belong to a large protein family (PF09203) that forms MspA-like cell wall channels. These proteins are able to form channels in lipid bilayers.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Article
Microbiology
Celia de Sousa-d'Auria, Florence Constantinesco, Nicolas Bayan, Patricia Constant, Maryelle Tropis, Mamadou Daffe, Marc Graille, Christine Houssin
Summary: Mycolic acids are essential components of the cell envelope in Corynebacteriales, particularly in Mycobacterium tuberculosis. In this study, the researchers identified a new protein (Cg1246) involved in mycolic acid metabolism using mutagenesis. They characterized the functional and structural properties of Cg1246 and found that its absence led to a decrease in the pool of trehalose monomycolate (TMM), resulting in a reduction in other mycolate-containing compounds. Interestingly, the researchers discovered that the phosphatase activity of Cg1246 was not involved in mycolic acid biosynthesis.
Article
Biochemistry & Molecular Biology
Claudio Piselli, Vinaya Kumar Golla, Roland Benz, Ulrich Kleinekathoefer
Summary: This study focuses on the characterization of the lysine cluster in OprO, a porin protein in the outer membrane of Pseudomonas aeruginosa. The importance of lysine residues in ion conductance and phosphate ion flow was demonstrated through experiments and simulations. The results suggest that an improved understanding of lysine residues and ion mobility could lead to the development of more effective antibiotics for P. aeruginosa.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Chemistry, Medicinal
Abhishek Acharya, Ishan Ghai, Claudio Piselli, Jigneshkumar Dahyabhai Prajapati, Roland Benz, Mathias Winterhalter, Ulrich Kleinekathoefer
Summary: In this study, the molecular mechanism of an antibiotic permeating through a bacterial channel is revealed, with a focus on the role of conformational dynamics of the constriction loop. The dynamics of the L3 loop, specifically the F118 to S125 segment, at the constriction regions of the OmpF porin is investigated using temperature accelerated sliced sampling approach. The importance of hydrogen-bond stabilization in the conformational dynamics of the L3 loop is demonstrated through single channel electrophysiology experiments and applied-field simulations. The findings suggest that conformational dynamics of the constriction loop may play a general role in antibiotic permeation through porins.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)