期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 285, 期 52, 页码 41087-41099出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.153692
关键词
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资金
- National Institutes of Health [HL021644]
- American Heart Association [0815362G]
The 49-residue functional upstream domain (FUD) of Streptococcus pyogenes F1 adhesin interacts with fibronectin (FN) in a heretofore unknown manner that prevents assembly of a FN matrix. Biotinylated FUD (b-FUD) bound to adsorbed FN or its recombinant N-terminal 70-kDa fibrin-and gelatin-binding fragment (70K). Binding was blocked by FN or 70K, but not by fibrin-or gelatin-binding subfragments of 70K. Isothermal titration calorimetry showed that FUD binds with K-d values of 5.2 and 59 nM to soluble 70K and FN, respectively. We tested sets of FUD mutants and epitope-mapped monoclonal antibodies (mAbs) for ability to compete with b-FUD for binding to FN or to block FN assembly by cultured fibroblasts. Deletions or alanine substitutions throughout FUD caused loss of both activities. mAb 4D1 to the (FNI)-F-2 module had little effect, whereas mAb 7D5 to the (FNI)-F-4 module in the fibrin-binding region, 5C3 to the (FNI)-F-9 module in the gelatin-binding region, or L8 to the G-strand of (FNIII)-F-1 module adjacent to 9FNI caused loss of binding of b-FUD to FN and decreased FN assembly. Conversely, FUD blocked binding of 7D5, 5C3, or L8, but not of 4D1, to FN. Circular dichroism indicated that FUD binds to 70K by beta-strand addition, a possibility supported by modeling based on crystal structures of peptides bound to (FNI)-F-2-(FNI)-F-5 of the fibrin-binding domain and (FNI)-F-8-(FNI)-F-9 of the gelatin-binding domain. Thus, the interaction likely involves an extensive anti-parallel beta-zipper in which FUD interacts with the E-strands of (FNI)-F-2-(FNI)-F-5 and (FNI)-F-8-(FNI)-F-9.
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