Article
Cell Biology
Wang Ruifang, Zhu Yinxing, Ren Chenwei, Yang Shuaike, Tian Shan, Chen Huanchun, Jin Meilin, Zhou Hongbo
Summary: IAV infection induces mitophagy, with the PB1-F2 protein playing a key role in this process by translocating to mitochondria and interacting with TUFM and LC3B. PB1-F2-induced mitophagy leads to degradation of MAVS and suppression of type I interferon production, resulting in impaired cellular innate immunity, making it a potential therapeutic target.
Article
Multidisciplinary Sciences
Tarani Kanta Barman, Victor C. Huber, Jesse L. Bonin, Danielle Califano, Sharon L. Salmon, Andrew N. J. McKenzie, Dennis W. Metzger
Summary: This study reveals the interplay between host interferon (IFN)-gamma and viral PB1-F2 virulence protein in regulating the functions of innate lymphoid cells (ILCs) and T cells during influenza virus infection. In the absence of IFN-gamma, lung ILC2s initiate a robust IL-5 response, leading to improved tissue integrity and increased survival. However, the presence of functional PB1-F2 suppresses ILC2 responses and induces a dominant IL-13 CD8 T cell response. This study also demonstrates the importance of IFN-gamma in host resistance against lethal doses of the influenza virus.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Andrew J. Burnham, Jason Rafe Miller, Indresh Singh, Emily A. Billings, Margaret A. Rush, Gillian M. Air, Stephan Bour
Summary: This study reports the in-silico identification of novel isoforms of PB1-F2 and PA-X in influenza virus genomes from avian samples, indicating the benefit of using multiple algorithms during gene annotation.
Article
Biochemistry & Molecular Biology
Christophe Chevalier, Olivier Leymarie, Laura Sedano, Bruno Da Costa, Charles-Adrien Richard, Pauline Maisonnasse, Matthieu Refregiers, Frederic Jamme, Ronan Le Goffic
Summary: In this study, researchers investigated the correlation between PB1-F2 protein structures and the resulting inflammatory response in influenza A virus-infected mice. The results showed a proinflammatory effect of PB1-F2 fibers compared to other forms of the protein, leading to respiratory distress. This suggests that the virulence mechanisms of PB1-F2 may depend on its sequence and oligomerization status.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Virology
Joelle Mettier, Clementine Prompt, Elise Bruder, Bruno Da Costa, Christophe Chevalier, Ronan Le Goffic
Summary: A study compared the interactome of human and avian PB1-F2 proteins and found differences in their functions. The human PB1-F2 protein is involved in Rho GTPase signaling, while the avian PB1-F2 protein is involved in ribonucleoprotein complex biogenesis. PB1-F2 can interact with various protein complexes and exert different functions.
Article
Biochemistry & Molecular Biology
Daryl Zheng Hao Aw, Keng Kai Heng, Jovian Yee Han Heok, Xin Yang Kong, Hui Chen, Tong Zhang, Weiwei Zhai, Vincent T. K. Chow
Summary: This study compared the evolution of H3N2 influenza in different host cells and found specific mutations and viral quasispecies. The adaptation and virulence of the virus varied in different host species. However, there were no significant differences in the neutralizing antibody profiles of human-influenza-immune serum samples against different passaged viruses. More studies are needed to understand the complex relationships between H3N2 virus evolution, viral virulence changes, and low vaccine efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna Kozell, Aleksei Solomonov, Ulyana Shimanovich
Summary: In the past, ultrasound and protein complexes were considered unrelated topics. However, recent studies have focused on the ability of ultrasound to induce chemical modifications on proteins, leading to changes in protein-protein interactions and self-assembly. Despite limited understanding of these phenomena, this review provides a comprehensive analysis of the role of ultrasound in protein complexation, with a specific focus on amyloid self-assembly relevant to neurodegenerative disorders.
Article
Virology
Deena R. Blumenkrantz, Thomas Mehoke, Kathryn Shaw-Saliba, Harrison Powell, Nicholas Wohlgemuth, Hsuan Liu, Elizabeth Macias, Jared Evans, Mitra Lewis, Rebecca Medina, Justin Hardick, Lauren M. Sauer, Andrea Dugas, Anna DuVal, Andrew P. Lane, Charlotte Gaydos, Richard Rothman, Peter Thielen, Andrew Pekosz
Summary: The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade, which dominated subsequent seasons. Research found that a virus with the novel NAg+F2P genotype replicated less well in human nasal epithelial cell cultures, and viruses with this genotype were associated with increased cough and shortness of breath in infected patients based on retrospective analyses of clinical data.
Article
Virology
Deena R. Blumenkrantz, Thomas Mehoke, Kathryn Shaw-Saliba, Harrison Powell, Nicholas Wohlgemuth, Hsuan Liu, Elizabeth Macias, Jared Evans, Mitra Lewis, Rebecca Medina, Justin Hardick, Lauren M. Sauer, Andrea Dugas, Anna DuVal, Andrew P. Lane, Charlotte Gaydos, Richard Rothman, Peter Thielen, Andrew Pekosz
Summary: The 2014-15 influenza season witnessed the emergence of a new variant affecting the H3N2 virus, which dominated in subsequent flu seasons. This variant, associated with increased cough and shortness of breath in infected patients, had a presence of specific mutations in the NA and PB1-F2 genes.
Article
Biochemistry & Molecular Biology
Christina Mountaki, Ioannis Dafnis, Eirini A. Panagopoulou, Paraskevi B. Vasilakopoulou, Michalis Karvelas, Antonia Chiou, Vaios T. Karathanos, Angeliki Chroni
Summary: The study found that polar phenols can affect the AD-related pathogenic functions of apoE4 forms, with certain compounds preventing cellular changes by modulating redox status and improving the structure of apoE4 forms. This research provides evidence that natural compounds could modify the structure of apoE4 forms and ameliorate AD-related pathogenic effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sheng Zhang, Stan Yoo, Dalton T. Snyder, Benjamin B. Katz, Amy Henrickson, Borries Demeler, Vicki H. Wysocki, Adam G. Kreutzer, James S. Nowick
Summary: A beta dimers are neurotoxic and pathologically relevant species in Alzheimer's disease. This paper introduces a stable A beta(C18C33) dimer model that forms pure dimers in lipid environments without aggregation into fibrils. This model is important for amyloid and Alzheimer's disease research.
Article
Chemistry, Physical
Noorul Huda, Abani K. Bhuyan
Summary: This study explores the effects of electric field on the structure of proteins and finds that a DC field of 1.3 V cm(-1) leads to electrophoretic, electrochemical, and unfolding effects in cytochrome c within 90 minutes. The protein also undergoes denaturation and amorphous aggregation.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biochemical Research Methods
Carole Farre, Sara Viezzi, Alice Wright, Perrine Robin, Nathalie Lejal, Marisa Manzano, Jasmina Vidic, Carole Chaix
Summary: This study developed a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the comprehensive detection of Influenza A virus. The assay combined immunomagnetic beads and biotin-nanoparticle-based detection to quantify the viral nucleoprotein. The use of biotin-nanoparticles enabled low detection limits and the new MELISA method showed high specificity without cross-reactivity with a porcine respiratory virus.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Priya Vizzini, Marisa Manzano, Carole Farre, Thierry Meylheuc, Carole Chaix, Nalini Ramarao, Jasmina Vidic
Summary: By using biotinylated silica nanoparticles, the sensitivity of paper-based DNA biosensors was significantly improved, enabling rapid screening of food containing Campylobacter DNA without the need for a PCR step.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Ozgun Kocabiyik, Valeria Cagno, Paulo Jacob Silva, Yong Zhu, Laura Sedano, Yoshita Bhide, Joelle Mettier, Chiara Medaglia, Bruno Da Costa, Samuel Constant, Song Huang, Laurent Kaiser, Wouter L. J. Hinrichs, Anke Huckeriede, Ronan Le Goffic, Caroline Tapparel, Francesco Stellacci
Summary: This study describes a new class of broad-spectrum anti-influenza macromolecules that meet the criteria of targeting conserved viral domains and being virucidal with low toxicity. These compounds based on cyclodextrin core show effective inhibition of viruses and irreversibility of inhibition. In vitro efficacy is demonstrated against various human or avian influenza strains, as well as a 2009 pandemic strain ex vivo. Furthermore, therapeutic efficacy is shown in mice with one compound providing 90% survival when administered post-infection.
Article
Engineering, Biomedical
Ximena Zottig, Soultan Al-Halifa, Melanie Cote-Cyr, Cynthia Calzas, Ronan Le Goffic, Christophe Chevalier, Denis Archambault, Steve Bourgault
Summary: Proteinaceous nanostructures, particularly synthetic beta-sheet self-assembling peptides, show promise in developing subunit vaccines. By controlling the self-assembly process using electrostatic capping motifs, uniform nanorods displaying specific epitopes have been successfully created. These nanorods are efficiently taken up by antigen presenting cells, activate immune responses, and provide protection against lethal infections, demonstrating their potential as a versatile delivery platform for subunit vaccines.
Review
Food Science & Technology
Nejra Omerovic, Mila Djisalov, Kristina Zivojevic, Minja Mladenovic, Jovana Vunduk, Ivanka Milenkovic, Nikola Z. Knezevic, Ivana Gadjanski, Jasmina Vidic
Summary: The food industry faces challenges in providing tasty and convenient food with extended shelf life. Antimicrobial composite materials for active food packaging, combining efficient antibacterial nanoparticles with environmentally friendly polymers, can significantly reduce the risk of foodborne pathogen outbreaks, improve food safety and quality, minimize product losses, and maintain sustainability.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Review
Food Science & Technology
Delphine Cormontagne, Virginie Rigourd, Jasmina Vidic, Francesco Rizzotto, Emmanuelle Bille, Nalini Ramarao
Summary: Human breast milk is crucial for infants, especially preterm neonates, but the risk of spoilage due to contamination by pathogens, particularly Bacillus cereus, exists. Bacillus cereus can contaminate milk at all stages and cause severe infections in newborns with very low birth weight, highlighting the importance of strict procedures in milk banks to prevent contamination and ensure safety.
Article
Food Science & Technology
Simone Balbinot, Anand Mohan Srivastav, Jasmina Vidic, Ibrahim Abdulhalim, Marisa Manzano
Summary: This article highlights the importance and advantages of optical biosensors in food safety, comparing them with traditional methods and showcasing the applications of different technologies and future trends.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Review
Chemistry, Physical
Jasmina Vidic, Marisa Manzano
Summary: Electrochemical biosensors offer a rapid and accurate means of detecting pathogens, with applications in clinical, environmental, and food industry sectors. Utilizing screen-printed electrodes in conjunction with nanoparticles can enhance signal and sensitivity, while functionalizing the working electrodes ensures highly specific pathogen detection.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Review
Environmental Sciences
Zoi Kotsiri, Jasmina Vidic, Apostolos Vantarakis
Summary: Biosensors are valuable for detecting foodborne and waterborne pathogens due to their portability, usability, and real-time response. However, current biosensors lack sensitivity and specificity in complex matrices. A systematic review of biosensor applications in identifying microorganisms was conducted, revealing ongoing efforts to overcome detection limitations and safeguard water and food quality. Biosensors are essential tools for evaluating and monitoring the environment and food, with significant impact on public health.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Antoine Guillon, Deborah Brea-Diakite, Adeline Cezard, Alan Wacquiez, Thomas Baranek, Jerome Bourgeais, Frederic Picou, Virginie Vasseur, Lea Meyer, Christophe Chevalier, Adrien Auvet, Jose M. Carballido, Lydie Nadal Desbarats, Florent Dingli, Andrei Turtoi, Audrey Le Gouellec, Florence Fauvelle, Amelie Donchet, Thibaut Crepin, Pieter S. Hiemstra, Christophe Paget, Damarys Loew, Olivier Herault, Nadia Naffakh, Ronan Le Goffic, Mustapha Si-Tahar
Summary: Research has shown that influenza virus infection causes significant changes in lung metabolism, leading to the accumulation of succinate in respiratory fluids. Succinate exhibits powerful antiviral activity by inhibiting influenza virus replication and reducing metabolic disruptions and inflammatory responses. Administering succinate intranasally to mice reduces viral loads in the lungs and improves survival rates. The antiviral mechanism involves succinylation of viral nucleoproteins, which alters their interactions with viral RNA.
Article
Nutrition & Dietetics
Elliot Mathieu, Quentin Marquant, Florian Chain, Edwige Bouguyon, Vinciane Saint-Criq, Ronan Le-Goffic, Delphyne Descamps, Philippe Langella, Thomas A. A. Tompkins, Sylvie Binda, Muriel Thomas
Summary: Microbiota studies have shown that certain strains of Streptococcus have potential antimicrobial properties against respiratory pathogens. In this study, a Streptococcus mitis strain from a human newborn was found to have in vitro antimicrobial activity against non-typable Haemophilus influenza, Pseudomonas aeruginosa, and Staphylococcus aureus. However, further research is needed to determine its efficacy in protecting against respiratory infections, as treatment with this strain did not provide effective protection against NTHi lung infection and may have even exacerbated the infection.
Review
Chemistry, Analytical
Vanessa Escobar, Natale Scaramozzino, Jasmina Vidic, Arnaud Buhot, Raphael Mathey, Carole Chaix, Yanxia Hou
Summary: Foodborne pathogens cause a significant number of illnesses worldwide. In recent decades, there has been a growing development of accurate biosensors for detecting bacterial pathogens in food, using peptides as recognition biomolecules. This review discusses strategies for designing and screening sensitive peptide bioreceptors, as well as advanced techniques for peptide-based biosensors and innovative approaches like electronic noses for food monitoring. These biosensors and electronic noses offer promising alternatives for highly sensitive, cost-effective, and rapid pathogen detection, some of which can be used as portable devices for on-site analysis.
Article
Biochemistry & Molecular Biology
Jan Bohl, Mohammed Moudjou, Laetitia Herzog, Fabienne Reine, Fiona Sailer, Hannah Klute, Frederic Halgand, Guillaume Van der Rest, Yves Boulard, Vincent Beringue, Angelique Igel, Human Rezaei
Summary: Studies have shown that all mammalian prion assemblies exhibit the property of spontaneously generating two sets of discreet infectious tetrameric and dimeric species, which differ significantly in their specific infectivity. These oligomeric species are highly dynamic and evolve differently in the presence of chaotropic agents. The diversity in the quaternary structure of prion assemblies suggests that the structure of PrPSc can be divided into two independent folding domains.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Chemistry, Analytical
Francesco Rizzotto, Majd Khalife, Yanxia Hou, Carole Chaix, Florence Lagarde, Natale Scaramozzino, Jasmina Vidic
Summary: The rapid and sensitive detection of food contaminants is increasingly important for preventing and treating foodborne diseases. Electrochemical biosensors have emerged as convenient, rapid, sensitive, and user-friendly analytical devices for food safety analysis, thanks to their analytical characteristics and advances in bioreceptor design and production. These biosensors can detect food contaminants such as allergens, pesticides, antibiotic traces, toxins, and bacteria with a low limit of detection.
Article
Clinical Neurology
Davy Martin, Fabienne Reine, Laetitia Herzog, Angelique Igel-Egalon, Naima Aron, Christel Michel, Mohammed Moudjou, Guillaume Fichet, Isabelle Quadrio, Armand Perret-Liaudet, Olivier Andreoletti, Human Rezaei, Vincent Beringue
Summary: The study found that prions responsible for the most common form of sporadic Creutzfeldt-Jakob disease in humans can persist in the brain of transgenic mice for their entire life, potentially showing disease potency upon transmission to susceptible hosts. This highlights the ability of prions to persist and regain activity in non-replicative environments, raising concerns about the risk of indefinite prion persistence when utilizing therapeutic strategies aiming to lower PrP levels.
BRAIN COMMUNICATIONS
(2021)