Article
Plant Sciences
Zeyu Chen, Junwei Yuan, Yi Yao, Jiahao Cao, Wenjie Yang, Yu Long, Juanxu Liu, Weiyuan Yang
Summary: In this study, the authors identified the petunia anthocyanin acyltransferase gene, PhAAT1, which is located in the cytoplasm. Silencing of PhAAT1 resulted in changes in flower color and reduced the stability of anthocyanins. Metabolomics analysis showed a decrease in p-coumaroylated and caffeoylated anthocyanins upon PhAAT1 silencing. Additionally, PhAAT1 was found to be positively regulated by the MYB transcription factor, PhAN2, which directly interacts with the promoter of PhAAT1.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Juan Alejandro Perdomo, Peter Buchner, Elizabete Carmo-Silva
Summary: The relative abundance of redox-sensitive and redox-insensitive Rca isoforms is differentially regulated throughout the light-dark diel cycle in wheat, with TaRca2-beta and TaRca2-alpha transcript levels higher at the start of the day. Meanwhile, the abundance of Rubisco decreases by up to 20% during the dark period in fully expanded wheat leaves, suggesting post-transcriptional regulation of these photosynthetic enzymes.
PHOTOSYNTHESIS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Fangting Zhou, Xinyang Fan, Yongwang Miao
Summary: This study reveals that the LPIN1 gene is involved in milk fat synthesis in BMECs, and one of the important pathways is through direct transcriptional regulation of PPARG, which significantly affects the content of TAG in BMECs.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Alex Mas Monteys, Amiel A. Hundley, Paul T. Ranum, Luis Tecedor, Amy Muehlmatt, Euyn Lim, Dmitriy Lukashev, Rajeev Sivasankaran, Beverly L. Davidson
Summary: A universal switch element has been developed for precise control of gene replacement or editing machinery after exposure to small molecules. This switch system, called X-on, allows controlled protein expression by adjusting drug dose, protein stability, and redosing. It offers an opportunity to refine and tailor gene therapies in humans due to its oral bioavailability and safety of the drugs used.
Article
Biochemistry & Molecular Biology
Souichi Oe, Shinichi Hayashi, Susumu Tanaka, Taro Koike, Yukie Hirahara, Rio Kakizaki, Sumika Sakamoto, Yasuko Noda, Hisao Yamada, Masaaki Kitada
Summary: Our findings suggest that CPEB1 regulates Cpeb1 expression by repressing its translation, and miR-145a-5p and let-7b-5p are involved in this process, indicating that Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs.
Article
Biochemistry & Molecular Biology
Alice Shwe, Aleksei Krasnov, Tina Visnovska, Sigmund Ramberg, Tone-Kari K. Ostbye, Rune Andreassen
Summary: Smoltification is a complex developmental process in Atlantic salmon that involves remodeling of the gill. This study investigated the expression changes of miRNAs and mRNAs during smoltification and subsequent sea water adaptation. The results identified differentially expressed miRNAs that target genes related to various biological processes associated with smoltification and sea water adaptation. These findings suggest that post-transcriptional regulation by miRNAs plays a role in the remodeling of the gill during smoltification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xiaoshuang Zhou, Dongtai Chen, Yan Yan, Qiang Li, Wei Xing, Yanling Liu, Yonghua Chen, Dongyin Wang, Yunfei Yuan, Jingdun Xie, Weian Zeng, Jiahao Pan
Summary: This study reveals that NOP plays an oncogenic role in hepatocellular carcinoma (HCC) and is associated with clinicopathological features and survival outcomes of HCC patients. NOP overexpression promotes HCC growth, while its antagonist, JTC801, inhibits NOP function and could potentially be used as a drug for HCC therapy.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Daniela Boehm, Victor Lam, Martina Schnolzer, Melanie Ott
Summary: SMYD5 is identified as a host factor required for HIV-1 transcription, which binds to the HIV-1 promoter and activates transcription. Tat and USP11 stabilize the function of SMYD5.
Article
Plant Sciences
Weimin Liu, Siyan Liu, Kaiyue Zhang, Mingwei Xie, Haiwei Sun, Xiaoqin Huang, Lixia Zhang, Min Li
Summary: This study identified the key chlorophyll metabolism gene CsCLH1 in tea leaves and elucidated its regulatory mechanism. The DOF protein CsDOF3 was found to be an upstream direct regulator of CsCLH1, and the two proteins interacted to regulate chlorophyll synthesis in tea leaves.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Cell Biology
Julie Weidner, Carina Malmhall, Vahid Arabkari, Aidan Barrett, Emma Boberg, Linda Ekerljung, Madeleine Radinger
Summary: The polarization of CD4+ T cells into different T helper subsets is a crucial process in many diseases, including asthma. miR-19a has been identified as a regulator in T cell polarization by negatively controlling the expression of the SGK1 gene, therefore affecting T cell development. Thus, miR-19a plays an important role in the binding and regulation of SGK1 transcript levels during T cell development.
Article
Biochemistry & Molecular Biology
Takahiro Fukui, Keisuke Shoji, Takashi Kiuchi, Yutaka Suzuki, Susumu Katsuma
Summary: Lepidopteran insects show a different mechanism of sex determination compared to most insect species. The silkworm Bombyx mori, a model species in this group, has a sex-determining gene called Feminizer (Fem) on the female-specific W chromosome. Fem is a precursor of PIWI-interacting small RNA (piRNA) that cleaves the mRNA of the male-determining gene Masc, leading to female development. However, this piRNA-dependent sex determination strategy is not observed in the Asian corn borer, Ostrinia furnacalis. Despite previous findings of masculinizing function of the Masc gene (OfMasc) in O. furnacalis embryos, there was no difference in the expression level of OfMasc between males and females during sex determination. Furthermore, deep sequencing analysis did not identify any female-specific small RNAs mapped onto OfMasc mRNA. Knockdown of two PIWI genes also did not affect the expression level of OfMasc in either sex. These findings suggest the possibility of divergent evolution of sex determinants in Lepidoptera.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Sheng Huang, Noriyuki Konishi, Naoki Yamaji, Ji Feng Shao, Namiki Mitani-Ueno, Jian Feng Ma
Summary: Boron uptake in rice is mediated by two transporters, OsLsi1 and OsBOR1, with polar localization cooperating for efficient uptake. OsBOR1 undergoes degradation in response to high B, while OsLsi1 remains unaffected. This distinct regulatory mechanism in rice involves clathrin-independent degradation of OsBOR1 in contrast to Arabidopsis thaliana.
Article
Biochemistry & Molecular Biology
Hai-Ting Liu, Yong-Xin Zou, Wen-Jie Zhu, Sen-Liu, Guo-Hao Zhang, Ran-Ran Ma, Xiang-Yu Guo, Peng Gao
Summary: This study identified that THAP7-AS1 is highly expressed in gastric cancer, associated with lymph node metastasis and poor prognosis, and promotes gastric cancer progression through pathways involving SP1 and METTL3. These findings suggest that THAP7-AS1 may be a potential molecular target for gastric cancer therapy.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Multidisciplinary Sciences
Maorong Wen, Yunlei Cao, Bin Wu, Taoran Xiao, Ruiyu Cao, Qian Wang, Xiwei Liu, Hongjuan Xue, Yang Yu, Jialing Lin, Chenqi Xu, Jie Xu, Bo OuYang
Summary: The cytoplasmic domain of PD-L1 (PD-L1-CD) is involved in regulating PD-L1 stability and degradation. Here the authors show that membrane binding of PD-L1-CD mediates the cellular levels of PD-L1, while metformin can disrupt the interaction between PD-L1-CD and the membrane to reduce PD-L1 levels.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Elliot T. Martin, Kahini Sarkar, Alicia McCarthy, Prashanth Rangan
Summary: Understanding how stem cell differentiation is regulated is crucial for degenerative disease research and regenerative therapy design. In this study, researchers developed a tool called Oo-site to visualize bulk RNA-seq, single-cell RNA-seq, and translational efficiency data during different stages of germline differentiation in Drosophila ovary. They successfully recapitulated known expression patterns of developmentally regulated genes and discovered translational regulation of meiotic genes.
Letter
Infectious Diseases
Frederic Robin, Racha Beyrouthy, Julien Colot, Pierre Saint-Sardos, Anne Berger-Carbonne, Guillaume Dalmasso, Julien Delmas, Richard Bonnet
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2017)
Review
Food Science & Technology
Tiphanie Fais, Julien Delmas, Nicolas Barnich, Richard Bonnet, Guillaume Dalmasso
Article
Multidisciplinary Sciences
Alexis Bretin, Cecily Lucas, Anais Larabi, Guillaume Dalmasso, Elisabeth Billard, Nicolas Barnich, Richard Bonnet, Hang Thi Thu Nguyen
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Guillaume Dalmasso, Hang T. T. Nguyen, Tiphanie Fais, Sebastien Massier, Nicolas Barnich, Julien Delmas, Richard Bonnet
Article
Gastroenterology & Hepatology
Cecily Lucas, Laurene Salesse, My Hanh Thi Hoang, Mathilde Bonnet, Pierre Sauvanet, Anais Larabi, Catherine Godfraind, Johan Gagniere, Denis Pezet, Philip Rosenstiel, Nicolas Barnich, Richard Bonnet, Guillaume Dalmasso, Hang Thi Thu Nguyen
Article
Multidisciplinary Sciences
Cayetano Pleguezuelos-Manzano, Jens Puschhof, Axel Rosendahl Huber, Arne van Hoeck, Henry M. Wood, Jason Nomburg, Carino Gurjao, Freek Manders, Guillaume Dalmasso, Paul B. Stege, Fernanda L. Paganelli, Maarten H. Geurts, Joep Beumer, Tomohiro Mizutani, Yi Miao, Reinier van der Linden, Stefan van der Elst, K. Christopher Garcia, Janetta Top, Rob J. L. Willems, Marios Giannakis, Richard Bonnet, Phil Quirke, Matthew Meyerson, Edwin Cuppen, Ruben van Boxtel, Hans Clevers
Article
Gastroenterology & Hepatology
Anais Larabi, Guillaume Dalmasso, Julien Delmas, Nicolas Barnich, Hang Thi Thu Nguyen
Article
Microbiology
Anais Larabi, Laurene Salesse, Charlotte Cordonnier, Lucie Etienne-Mesmin, Nicolas Barnich, Guillaume Dalmasso, Hang Thi Thu Nguyen
Review
Microbiology
Mickael Desvaux, Guillaume Dalmasso, Racha Beyrouthy, Nicolas Barnich, Julien Delmas, Richard Bonnet
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Guillaume Dalmasso, Hang Thi Thu Nguyen, Tiphanie Fais, Sebastien Massier, Caroline Chevarin, Emilie Vazeille, Nicolas Barnich, Julien Delmas, Richard Bonnet
Summary: The study revealed that the yersiniabactin siderophore of AIEC provides an advantage for growth in competitive environments, but it also activates autophagy leading to bacterial clearance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Laurene Salesse, Cecily Lucas, My Hanh Thi Hoang, Pierre Sauvanet, Alexandra Rezard, Philip Rosenstiel, Christelle Damon-Soubeyrand, Nicolas Barnich, Catherine Godfraind, Guillaume Dalmasso, Hang Thi Thu Nguyen
Summary: Colorectal cancer patients show changes in intestinal flora with an increase in harmful bacteria such as Escherichia coli, which can cause DNA damage. Autophagy is a crucial cell defense mechanism that inhibits tumorigenesis. Targeting autophagy may be a promising strategy to counteract the protumoral effects of bacteria like E. coli producing colibactin.
Article
Microbiology
Richard Bonnet, Racha Beyrouthy, Marisa Haenni, Marie-Helene Nicolas-Chanoine, Guillaume Dalmasso, Jean-Yves Madec
Summary: This study investigated the presence of multidrug-resistant Escherichia coli ST131 in animal reservoirs and identified genetic features associated with different hosts, including virulence factors and metabolic functions. The research revealed a link between host, ST131 population structure, and virulence factor content, indicating that host adaptation plays a key role in the evolution of ST131.
Article
Microbiology
Dalmasso Guillaume, Beyrouthy Racha, Brugiroux Sandrine, Ruppe Etienne, Guillouard Laurent, Bonnin Virginie, Saint-Sardos Pierre, Ghozlane Amine, Gaumet Vincent, Barnich Nicolas, Delmas Julien, Bonnet Richard
Summary: Without the use of antibiotics, the plasmid-mediated resistance gene mcr-1 enhances the colonization ability of Escherichia coli in the gut, but impairs its lethal effect. This improvement in gut fitness is associated with a downregulation of intestinal inflammatory markers and the preservation of intestinal microbiota composition.
Article
Immunology
Racha Beyrouthy, Guillaume Dalmasso, Aurelien Birer, Frederic Robin, Richard Bonnet
EMERGING INFECTIOUS DISEASES
(2020)
Article
Multidisciplinary Sciences
Julien Delmas, Lucie Gibold, Tiphanie Fais, Sylvine Batista, Martin Leremboure, Clara Sinel, Emilie Vazeille, Vincent Cattoir, Anthony Buisson, Nicolas Barnich, Guillaume Dalmasso, Richard Bonnet
SCIENTIFIC REPORTS
(2019)