Article
Chemistry, Applied
Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, Carlos Afonso, Patrice Lerouge, Muriel Bardor
Summary: Microalgae, such as diatoms, are emerging as production systems for recombinant proteins like monoclonal antibodies. Efficient analytical methodologies are required to characterize the N-glycosylation machinery of the host cell and the glycoprotein-based bio-pharmaceuticals produced by microalgae. This study found that the diatom Phaeodactylum tricornutum can synthesize human-like oligomannosides, making it an environmentally friendly and advantageous expression system for producing biopharmaceuticals.
CARBOHYDRATE POLYMERS
(2021)
Article
Plant Sciences
Xihui Xie, Hong Du, Jichen Chen, Muhammad Aslam, Wanna Wang, Weizhou Chen, Ping Li, Hua Du, Xiaojuan Liu
Summary: The study focused on N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum, identifying a total of 639 N-glycoproteins and classifying 69 N-glycans. The findings expand understanding of protein glycosylation and update the N-glycan database in microalgae, potentially aiding in the production of humanized biopharmaceutical N-glycoproteins.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Ananya Agarwal, Orly Levitan, Helena Cruz de Carvalho, Paul G. Falkowski
Summary: Unlike most plants, unicellular algae can adapt to changes in irradiance within a few hours to days through an enigmatic signaling pathway that coordinates changes in plastid and nuclear gene expression. Functional studies on the model diatom, Phaeodactylum tricornutum, identified two potential signal transduction molecules responsible for photoacclimation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Environmental Sciences
Sea-Yong Kim, Ulla Rasmussen, Sara Rydberg
Summary: In this study, the effect and function of the neurotoxin BMAA in the diatom Phaeodactylum tricornutum were investigated. The results showed that BMAA can control the growth of the diatom by triggering oxidative stress and influencing photosynthesis and nitrogen metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Vasco Giovagnetti, Alexander Ruban
Summary: The study investigated the fluorescence emission characteristics of photosystems in the pennate diatom, revealing that the F710 fluorescence originates from Photosystem I and is enhanced in cells grown under intermittent light. This enhancement is likely due to increased energy flow from the FCP antenna to Photosystem I.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Andrew Diamond, Aracely Maribel Diaz-Garza, Jessica Li, Samuel S. Slattery, Natacha Merindol, Elisa Fantino, Fatma Meddeb-Mouelhi, Bogumil J. Karas, Simon Barnabe, Isabel Desgagne-Penix
Summary: The efficacy and limitations of the episome expression system in Phaeodactylum tricornutum were studied, revealing issues such as episome rearrangement and toxicity of fusion proteins.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Medicinal
Victor Murison, Josiane Herault, Benoit Schoefs, Justine Marchand, Lionel Ulmann
Summary: Through genomic analysis, researchers have made progress in understanding the lipid degradation mechanisms of oleaginous diatoms, identifying 57 potential enzymes involved. Further analysis revealed that 9 genes were highly regulated under nitrogen starvation conditions, suggesting an important role in lipid degradation. Tertiary structure prediction highlighted two downregulated enzymes as potential targets for future functional genomics and purification studies to investigate their role in lipid degradation.
Article
Biotechnology & Applied Microbiology
Wenting Yanga, Lu Zhou, Jintao Zhuo, Xin Li, Songcui Wu, Guihong Yu, Shan Gao, Guangce Wang
Summary: This study demonstrates the successful production of secreted AMPs using P. tricornutum as a cell factory. The antibacterial effects of the secreted AMPs were confirmed through bacteriostatic experiments.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biodiversity Conservation
Haodong Luo, Wanying Ma, Hao Fang, Zidong Liu, Syed Shabi Ul Hassan Kazmi, Yanmei Fan, Zhen Wang, Xiaojuan Liu
Summary: In this study, bZIP transcription factors (TFs) in the diatom Phaeodactylum tricornutum (Pt) were analyzed using bioinformatic and molecular methods. Twenty-two PtbZIP genes were identified and classified into seven clades based on phylogenetic relationships, conserved motifs, and gene structures. Among them, PtbZIP12 and PtbZIP13 were identified as a putative pair of duplicated genes. It was also observed that laminarin content showed a circadian rhythm. Based on these findings, a regulatory mechanism for laminarin biosynthesis was proposed in P. tricornutum.
ECOLOGICAL INDICATORS
(2023)
Article
Biochemical Research Methods
Tessema K. Kassaw, Andrew J. Paton, Graham Peers
Summary: In this study, a highly efficient, tunable, and reversible episome-based transcriptional control system was developed in the model diatom alga, Phaeodactylum tricornutum. The system is controlled by beta-estradiol and digoxin and exhibits a high dynamic range.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xihui Xie, Jianchao Yang, Hong Du, Jichen Chen, Edmond Sanganyado, Yangmin Gong, Hua Du, Weizhou Chen, Zhengyi Liu, Xiaojuan Liu
Summary: The knockout of PtFucT1 in P. tricornutum resulted in the inhibition of algal growth, biomass, and photosynthesis efficiency. PtFucT1 plays a crucial role in non-core fucose modification of N-glycans, and its absence could affect the activity of PtGnTI and alter the N-glycan structure. This study provides valuable insights into the mechanism of protein N-glycosylation modification and the potential use of microalgae as ecofriendly cell factories for biopharmaceutical production.
MICROBIAL CELL FACTORIES
(2023)
Article
Plant Sciences
Jochen M. Buck, Marie Wunsch, Alexander F. Schober, Peter G. Kroth, Bernard Lepetit
Summary: Iron is a crucial factor in the growth and photoprotection mechanism of diatoms. We found that iron limitation leads to upregulation of Lhcx2 and enhances qE effect. In addition to changes in Lhcx2, iron limitation also causes other adaptive changes in diatoms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ben Leyland, Ekaterina Novichkova, Achintya Kumar Dolui, Denis Jallet, Fayza Daboussi, Bertrand Legeret, Zhongze Li, Yonghua Li-Beisson, Sammy Boussiba, Inna Khozin-Goldberg
Summary: Diatoms can accumulate and utilize neutral storage lipids, which are crucial for their dominance in marine ecosystems. A protein called PtACBP has been identified to play a role in lipid metabolism and affect various cellular processes in diatoms.
Article
Environmental Sciences
Marta Sendra, Araceli Rodriguez-Romero, Maria Pilar Yeste, Julian Blasco, Antonio Tovar-Sanchez
Summary: This study found that the components of regular surgical masks may be toxic to marine life. The release rate of polypropylene microfibres and metals from these masks in marine water was studied, and the toxicity of these by-products on a marine diatom was assessed. The results showed that microfibres were released into the water after one month, and dissolved metals were detected in the water containing the mask fragments. The water from the mask fragments had a significant dose-dependent decrease in cell density, while the water from the whole mask showed changes in the photosynthetic apparatus and intrinsic properties of the cells.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Jie Ma, Fengyuan Chen, Beibei Zhou, Zhen Zhang, Ke Pan
Summary: This study investigated the effects of nitrogen and phosphorus on the cell surface and metal toxicity in the marine diatom Phaeodactylum tricornutum. The results showed that higher nitrogen and phosphorus supply enhanced the adsorption and uptake of cadmium in the diatom cells. The increased ion-binding sites on the cell surface and up-regulated transporter genes were identified as the mechanisms responsible for the enhanced cadmium uptake.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Lena Petit, Lea Vernes, Jean-Paul Cadoret
Summary: This study used computational tools to investigate molecules derived from algae, identifying several promising compounds with potential antiviral properties, including phycocyanobilin, phycoerythrobilin, phycourobilin, and folic acid. These compounds showed reliable binding energies and are likely to compete with the SARS-CoV-2/ACE2 complex in further studies.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Review
Cell Biology
Giorgia Chinigo, Helene Castel, Oana Chever, Dimitra Gkika
Summary: Malignant glioma, especially glioblastoma (GBM), is a common primary brain tumor with high resistance to standard treatment. Recent studies have identified TRP channels as potential new molecular targets for glioma treatment, offering directions for innovative strategies to overcome high resistance and recurrence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Charlotte Toustou, Marie-Laure Walet-Balieu, Marie-Christine Kiefer-Meyer, Marine Houdou, Patrice Lerouge, Francois Foulquier, Muriel Bardor
Summary: N-glycosylation is an important post-translational modification that is highly conserved in eukaryotes, bacteria, and archaea. The processing of N-glycans in eukaryotes is sequential and involves specific steps in the secretory pathway. Recent research has also highlighted the regulation of N-glycosylation, including the biosynthesis of complex-type N-glycans and the role of TMEM165 in different eukaryotic clades.
BIOLOGICAL REVIEWS
(2022)
Article
Cell Biology
Daniele Campisi, Laurence Desrues, Kleouforo-Paul Dembele, Alexandre Mutel, Renaud Parment, Pierrick Gandolfo, Helene Castel, Fabrice Morin
Summary: ATG9A protein plays a critical role in chemotactic migration by promoting the formation of lamellipodia and the adhesion of cells to the extracellular matrix.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Plant Sciences
Anne Pajot, Johann Lavaud, Gregory Carrier, Matthieu Garnier, Bruno Saint-Jean, Noemie Rabilloud, Caroline Baroukh, Jean-Baptiste Berard, Olivier Bernard, Luc Marchal, Elodie Nicolau
Summary: Differences in lhc classification in Chromista were observed. A classification of the lhcf family with specific groups for haptophytes, diatoms, and seaweeds was proposed. Similarity analysis was performed to identify and characterize Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) in the haptophyte microalgae Tisochrysis lutea. The study revealed dynamic changes in lhc gene expression with nitrogen depletion and the exclusive nighttime expression of the lhcx2 gene in T. lutea, suggesting its role in protecting cells from light exposure after prolonged darkness.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Cell Biology
Daniele Campisi, Laurence Desrues, Kleouforo-Paul Dembele, Alexandre Mutel, Renaud Parment, Pierrick Gandolfo, Helene Castel, Fabrice Morin
Summary: ATG9A protein contributes to cell chemotaxis and controls cell protrusion and adhesion dynamics through interactions with other proteins.
Article
Biotechnology & Applied Microbiology
H. Bonnefond, Y. Lie, T. Lacour, B. Saint-Jean, G. Carrier, E. Pruvost, A. Talec, O. Bernard, A. Sciandra
Summary: Species domestication and improvement are important for increasing agricultural production. The microalgae industry can follow the same path by selecting and improving microalgal strains. The use of competition in a dynamic environment can drive the evolution and emergence of new adapted populations with enhanced properties. By selecting microalgae strains under variable light intensity, we successfully increased biomass productivity and observed the appearance of new alleles.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Oncology
Renaud Parment, Martine Dubois, Laurence Desrues, Alexandre Mutel, Kleouforo-Paul Dembele, Nicolas Belin, Laure Tron, Charlene Guerin, Moise Coeffier, Vincent Compere, Celine Feger, Florence Joly, Pascal Hilber, David Ribet, Helene Castel
Summary: Qiseng (R) has a preventive effect on fatigue and cognitive impairment caused by chemotherapy. It also regulates gut microbiota and inhibits inflammation.
Article
Oncology
Marie Lange, Benedicte Clarisse, Alexandra Leconte, Kleouforo-Paul Dembele, Justine Lequesne, Celeste Nicola, Martine Dubois, Laurence Derues, Yori Gidron, Helene Castel, Florence Joly
Summary: This study aims to evaluate the impact of immunotherapy on cognitive function in cancer patients without simultaneous anti-cancer treatment and to explore its effects on quality of life. The study will assess the incidence of cognitive decline and investigate secondary endpoints such as anxiety, depression, fatigue, clinical characteristics, biological data, and neurophysiological measures.
Review
Oncology
Charlotte Sleurs, Ali Amidi, Lisa M. Wu, David Kiesl, Philipp Zimmer, Marie Lange, Anne Rogiers, Benedicte Giffard, Giulia Binarelli, Cindy Borghgraef, Sabine Deprez, Mylene Duivon, Michiel De Ruiter, Sanne Schagen, Djihane Ahmed-Lecheheb, Helene Castel, Cecilie R. Buskbjerg, Melanie Dos Santos, Florence Joly, Joy Perrier
Summary: Cancer-related cognitive impairment (CRCI) has been increasingly identified in non-CNS system cancer patients over the last two decades in Europe. Researchers in Europe have contributed to the field by developing preclinical models and exploring underlying mechanisms. New challenges need to be addressed, including data sharing, standardization of assessments, and assessing additional biomarkers and neuroimaging investigations. This consensus paper summarizes the current state of CRCI research in Europe and proposes specific actions for future intervention programs based on shared scientific expert opinion.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Jeremy Berthelier, Bruno Saint-Jean, Nathalie Casse, Gael Bougaran, Gregory Carrier
Summary: Microalgal domestication is an expanding research field that aims to maximize the potential of microalgae for biotechnological purposes. In this study, we investigated the stability and genetic changes of a domesticated strain of Tisochrysis lutea, which showed improved lipid traits compared to the native strain. We found specific genetic variations between the two strains and highlighted the role of transposable elements in the domesticated strain's improved phenotype.
Article
Biotechnology & Applied Microbiology
Judith Rumin, Gregory Carrier, Catherine Rouxel, Aurelie Charrier, Virginie Raimbault, Jean-Paul Cadoret, Gael Bougaran, Bruno Saint-Jean
Summary: This study evaluates the impact of random mutagenesis on the genetic diversity of populations of the diatom Phaeodactylum tricornutum. The results show that out of the five populations obtained following EMS treatment, only one had high genetic diversity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Medicinal
Taraneh Sadat Zavvar, Anton Amadeus Hormann, Maximilian Klingler, Dominik Summer, Christine Rangger, Laurence Desrues, Helene Castel, Pierrick Gandolfo, Elisabeth von Guggenberg
Summary: Different modifications were explored for the minigastrin analog DOTA-MGS5, and five new derivatives were synthesized for radiolabeling. The new derivatives showed high receptor affinity and resistance against enzymatic degradation. Among them, [In-111]In-DOTA-[(N-Me)1Nal(8)]MGS5 exhibited the most promising targeting properties.
Review
Oncology
Andreas Bikfalvi, Cristine Alves da Costa, Tony Avril, Jean-Vianney Barnier, Luc Bauchet, Lucie Brisson, Pierre Francois Cartron, Helene Castel, Eric Chevet, Herve Chneiweiss, Anne Clavreul, Bruno Constantin, Valerie Coronas, Thomas Daubon, Monique Dontenwill, Francois Ducray, Natacha Entz-Werle, Dominique Figarella-Branger, Isabelle Fournier, Jean-Sebastien Frenel, Mathieu Gabut, Thierry Galli, Julie Gavard, Gilles Huberfeld, Jean-Philippe Hugnot, Ahmed Idbaih, Marie-Pierre Junier, Thomas Mathivet, Philippe Menei, David Meyronet, Celine Mirjolet, Fabrice Morin, Jean Mosser, Elisabeth Cohen-Jonathan Moyal, Veronique Rousseau, Michel Salzet, Marc Sanson, Giorgio Seano, Emeline Tabouret, Aurelie Tchoghandjian, Laurent Turchi, Francois M. Vallette, Somya Vats, Maite Verreault, Thierry Virolle
Summary: Glioblastoma (GBM) is a highly lethal brain tumor, and recent studies have emphasized the importance of the tumor microenvironment (TME) as a therapeutic target. However, a comprehensive understanding of the different cellular and molecular components involved in the GBM TME and their interactions is still needed for the development of more effective treatments. This review presents a comprehensive report on the GBM TME, combining the contributions of researchers and physicians in France, and provides a holistic view of the subject by describing the specific features of the GBM TME at the cellular, molecular, and therapeutic levels.
Article
Chemistry, Organic
E. D. Sitsanidis, P. M. Kasapidou, J. R. Hiscock, V Gubala, H. Castel, P. I. A. Popoola, A. J. Hall, A. A. Edwards
Summary: In this study, we present the first diphenylalanine dipeptide hydrogelator capped with the cinnamoyl functional group (Cin-l-F-l-F) and evaluate its effect on molecular self-assembly and physical properties of the formed hydrogel. Furthermore, we report preliminary results of its cytotoxicity against glioblastoma (GBM) cancer cells.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)