Review
Cell Biology
Mengmeng Liu, Graham Goldman, Mary MacDougall, Shuo Chen
Summary: BMP signaling plays a crucial role in dentin development, regulating the differentiation and gene expression of odontoblasts through canonical and non-canonical Smad signaling pathways. Dysregulation of BMP signaling leads to tooth disorders, and studies in mice with BMP signaling-associated gene mutations or knockouts have provided insights into the underlying networks. This review summarizes recent advances in understanding BMP signaling in odontoblast differentiation and dentin formation, including the expression of BMPs, receptors, and downstream genes, as well as the structures of BMPs, receptors, antagonists, and their association with dentin defects.
Article
Physiology
Xiaoxiao Hu, Chensheng Lin, Ningsheng Ruan, Zhen Huang, Yanding Zhang, Xuefeng Hu
Summary: This study investigates the conservation of the atypical BMP canonical signaling pathway in human odontogenesis and reveals the functional operation of a pathway dependent on Smad1/5/8 but independent of Smad4 in human dental mesenchyme during early tooth development.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Muhammad S. Alam, Shizuka Otsuka, Nathan Wong, Aamna Abbasi, Matthias M. Gaida, Yu Fan, Daoud Meerzaman, Jonathan D. Ashwell
Summary: The study revealed that TNF can induce the differentiation of mouse CD4(+) T cells into proinflammatory Th17 cells, mediated by the nondeath TNF receptor TNFR2, and also has an impact on the generation of proinflammatory Th1 cells. Additionally, TNFR2-deficient CD4(+) T cells showed lower pathogenicity in EAE and colitis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Elif Tunc-Ozcan, Sarah M. Brooker, Jacqueline A. Bonds, Yung-Hsu Tsai, Radhika Rawat, Tammy L. McGuire, Chian-Yu Peng, John A. Kessler
Summary: Hippocampal BMP signaling is identified as a common pathway mediating the behavioral effects of multiple antidepressant classes and electroconvulsive therapy. Inhibition of hippocampal BMP signaling is shown to be both necessary and sufficient to mediate the effects of these treatments, involving a key role in neurogenesis in the hippocampus.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nae-Cherng Yang, Chia-Yu Chin, Ya-Xin Zheng, Inn Lee
Summary: This study investigates the mechanism by which Myo-Inositol (MI) alleviates aging in C. elegans. It is found that MI modulates the PI3K to attenuate the insulin/IGF-1 signaling pathway, ultimately leading to longevity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Hai-Ying Shen, Sadie B. Baer, Raey Gesese, John M. Cook, Landen Weltha, Shayla Q. Coffman, Jie Wu, Jiang-Fan Chen, Ming Gao, Teng Ji
Summary: This study aimed to evaluate the role of adenosine A(2A) receptor (A(2A)R) in the development of a SUDEP mouse model. The researchers found that the level of A(2A)R in the nucleus tractus solitarius (NTS) was increased in epileptic mice and that inhibiting A(2A)R could reduce SUDEP events. The findings suggest that A(2A)R may be a potential therapeutic target for preventing SUDEP.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pathology
Chenna Kesavulu Sugali, Naga Pradeep Rayana, Jiannong Dai, Michael Peng, Sherri L. Harris, Hannah C. Webber, Shaohui Liu, Stephan G. Dixon, Priyanka H. Parekh, Elizabeth A. Martin, Louis B. Cantor, Ronald L. Fellman, David G. Godfrey, Michelle R. Butler, Matthew E. Emanuel, Davinder S. Grover, Oluwatosin U. Smith, Abbot F. Clark, Vijay Krishna Raghunathan, Weiming Mao
Summary: This study identified that Dickkopf-related protein 1 (Dkk1) is elevated in the aqueous humor and trabecular meshwork (TM) of glaucoma patients, and found that Wnt and glucocorticoid receptor (GR) signaling inhibit each other in the TM. Activators of canonical Wnt signaling may prevent the adverse effects of glucocorticoids in the eye.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sang-Heon Kim, Jeong-Ah Jin, Hyung Joon So, Sung Hoon Lee, Tae-Wook Kang, Jae-Ung Lee, Dae Eun Choi, Jin Young Jeong, Yoon-Kyung Chang, Hyunsu Choi, Youngjun Lee, Young-Kwon Seo, Hong-Ki Lee
Summary: In this study, it was discovered that urine-derived stem cells (UDSCs) express Klotho protein more strongly than other mesenchymal stem cells (MSCs) and can suppress renal fibrosis by inhibiting transforming growth factor (TGF)-beta. This finding provides a potential new approach for treating chronic kidney disease (CKD).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Vijay Kondreddy, Jhansi Magisetty, Shiva Keshava, L. Vijaya Mohan Rao, Usha R. Pendurthi
Summary: The study aims to investigate the role of Gab2 in inflammatory signaling pathways and its contribution to vascular dysfunction. The results show that silencing Gab2 can significantly attenuate the expression of TF, cell adhesion molecules, and inflammatory cytokines/chemokines induced by cytokines.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Developmental Biology
Alessio Paolini, Dinara Sharipova, Tim Lange, Salim Abdelilah-Seyfried
Summary: This study reveals that the paralogous genes wnt9a/b and both canonical and non-canonical Wnt signaling pathways are required for the migration of cardiomyocyte progenitor cells and the formation of the cardiac tube in zebrafish. They regulate the timing of cardiac progenitor cell differentiation and activate downstream pathways to direct cardiac morphogenesis.
Article
Biochemistry & Molecular Biology
Rutambhara Purohit, Amal Kanti Bera
Summary: The interaction between Pannexin-1 (Panx1) channel and P2X7 receptor (P2X7R) has important cellular implications. ATP released through Panx1 activates P2X7R, which further activates Panx1. Previous studies have shown that Panx1-CT attenuates P2X7R-mediated Ca2+ influx and cell death. In this study, it was found that Panx1-CT inhibits P2X7R current, while full-length Panx1 initially reduces P2X7R current and then gradually increases it. Furthermore, Panx1-CT expression reduces mitochondrial depolarization, reactive oxygen species generation, Caspase 3 activation, and cell death, whereas overexpression of full-length Panx1 has the opposite effect. These findings indicate that Panx1 can modulate P2X7R-mediated cellular processes depending on the degree of P2X7R activation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Microbiology
Daniel S. Layton, Kostlend Mara, Meiling Dai, Luis Fernando Malaver-Ortega, Tamara J. Gough, Kerri Bruce, Kristie A. Jenkins, Andrew G. D. Bean
Summary: Influenza viruses pose a threat to human and poultry health. The study found that alterations in the interferon signaling pathway affect the cellular response to infection in chickens. This research contributes to the field of host-avian pathogen interaction and innate immunity in chickens.
Article
Multidisciplinary Sciences
Jing Hao, Xiangzi Han, Haidong Huang, Xingjiang Yu, Jiangkang Fang, Jianjun Zhao, Richard A. Prayson, Shideng Bao, Jennifer S. Yu
Summary: The Wnt pathway is frequently dysregulated in many cancers, underscoring it as a therapeutic target. Wnt inhibitors have uniformly failed in clinical trials. In this study, it is shown that Semaphorin 3C plays a role in activating the Wnt pathway through Rac1-dependent β-catenin nuclear accumulation, indicating that dual targeting of Semaphorin 3C and Wnt pathways may achieve clinically significant Wnt pathway inhibition.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Zengjie Zhang, Fangqian Wang, Xin Huang, Hangxiang Sun, Jianxiang Xu, Hao Qu, Xiaobo Yan, Wei Shi, Wangsiyuan Teng, Xiaoqiang Jin, Zhenxuan Shao, Yongxing Zhang, Shenzhi Zhao, Yan Wu, Zhaoming Ye, Xiaohua Yu
Summary: The role of sensory innervation in bone homeostasis is often overlooked in bone repair strategies. This study proposes a neuromodulation approach that uses engineered sensory nerves (eSN) to optimize bone defect healing by leveraging the adaptive capabilities of sensory neurons. The release of NGF from ECM-constructed eSN promotes sensory neuron differentiation and enhances CGRP secretion, leading to improved mobility of RAOECs and osteogenic differentiation of BMSCs. The eSN approach effectively drives ossification in vivo through the NGF-TrkA signaling pathway, accelerating the healing of critical size bone defects. It also suppresses excessive bone formation and promotes bone remodeling by activating osteoclasts via a CGRP-dependent mechanism when combined with BMP-2 delivery, mitigating the side effects of BMP-2. Overall, this eSN approach offers a valuable avenue for optimizing bone homeostasis in various clinical scenarios by harnessing the adaptive role of the neural system.
Article
Biochemistry & Molecular Biology
Hiroaki Okuda, Tatsuya Ishikawa, Kiyomi Hori, Nichakarn Kwankaew, Noriyuki Ozaki
Summary: This study investigates the potential role of the Hedgehog (Hh) signaling pathway in mechanical allodynia using a mouse model of neuropathic pain. The results show that the Hh signaling pathway can modulate neuronal activity in dorsal root ganglion (DRG) neurons via ATP release, and it plays an important role in sustaining mechanical allodynia and hypersensitivity in neuropathic pain.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Cell Biology
Hye-Rim Shin, Bong-Soo Kim, Hyun-Jung Kim, Heein Yoon, Woo-Jin Kim, Je-Yong Choi, Hyun-Mo Ryoo
Summary: This study finds that the main cause of limb shortening in Apert syndrome resulting from FGFR2 gene mutations is the excessive expression of RANKL, which leads to an imbalance in osteoblast and osteoclast coupling.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kyeong-Rok Kang, Jae-Sung Kim, Jeong-Yeon Seo, Hyang Lim, Tae-Hyeon Kim, Sun-Kyoung Yu, Heung-Joong Kim, Chun Sung Kim, Hong Sung Chun, Joo-Cheol Park, Do Kyung Kim
Summary: The present study investigates the role of NAMPT in odontogenic differentiation during tooth development in mice. The up-regulation of NAMPT promotes odontogenic differentiation and mineralization, while the inhibition of NAMPT attenuates odontogenic differentiation.
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hoon Seonwoo, Han-Wool Choung, Sangbae Park, Kyoung Soon Choi, Kyoung-Je Jang, Jangho Kim, Ki-Taek Lim, Yeonju Kim, Pankaj Garg, Shambhavi Pandey, Juo Lee, Joo-Cheol Park, Yun-Hoon Choung, Pill-Hoon Choung, Soo Young Kim, Jong Hoon Chung
Summary: Natural calcium phosphate cements (CPCs) derived from sintered animal bone have low mechanical strength, but incorporating reduced graphene oxide (RGO) and applying pulsed electromagnetic fields (PEMFs) can significantly improve their mechanical properties and osteogenic differentiation ability. The chemical bonding between RGO and CPCs enhances the mechanical properties, while PEMFs enhance osteogenic differentiation of stem cells. RGO-CPCs with PEMFs show improved biocompatibility and osteoinductivity in both in vitro and in vivo studies.
Article
Biochemistry & Molecular Biology
Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae- Moon, Eunji Lee, Jin-Muk Lim, Young-Dan Cho, Mi-Hye Lee, Hong-Gee Kim, Hyun-Mo Ryoo
Summary: Cellular senescence is closely related to tissue aging, including bone. Oxidative stress caused by the accumulation of reactive oxygen species (ROS) accelerates cellular senescence and dysfunction of osteoblasts. This study identified PADI2 as a regulator of ROS-accelerated senescence of osteoblasts and provided new insights into excessive ROS-promoted cellular senescence and aging-related bone diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shengfeng Bai, Ji-Hyun Lee, Chul Son, Dong-Seol Lee, Joo-Cheol Park
Summary: The study demonstrates that CPNE7 plays a regulatory role in periodontal ligament (PDL) repair by supporting PDL alignment through TAU-mediated cytoskeleton reorganization and direct regulation of CAP-mediated PDL attachments of PDLCs.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yoon Seon Lee, Yeoung-Hyun Park, You-Mi Seo, Hye-Kyung Lee, Joo-Cheol Park
Summary: This study identified that the Smad4-dependent TGF-beta 1/BMP2 signaling pathway regulates the expression of Cpne7 in dental epithelial cells, and the CPNE7 protein mediates odontoblast differentiation through epithelial-mesenchymal interaction. The results showed that conditioned medium treated with TGF-beta 1/BMP2 can induce the formation of tubular dentin.
Article
Dentistry, Oral Surgery & Medicine
Hye Ri Gug, Yeoung-Hyun Park, Su-Jin Park, Ji Yeon Jang, Ji-Hyun Lee, Dong-Seol Lee, Won -Jun Shon, Joo-Cheol Park
Summary: This study evaluated the potential application of CPNE7-derived functional peptide (CPNE7-DP) in dentin caries. The results showed that CPNE7-DP was stable in caries-like environments and regenerated tubular tertiary dentin, alleviating inflammation and reducing the expression of inflammatory cytokines. CPNE7-DP may help overcome the limitations of current restorative caries treatments.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Dong-Seol Lee, Yeo Joon Song, Hye Ri Gug, Ji-Hyun Lee, Hyun Sook Bae, Joo-Cheol Park
Summary: Tooth development involves cell divisions and interactions, and the NFI-C transcription factor plays a crucial role. This study reveals that Nfic regulates cell proliferation in various mineralized tissue stem cells, and is involved in the expression of pluripotency genes. Especially in dental epithelial stem cells, Nfic regulates epithelial cell proliferation through epithelial-mesenchymal interactions.
STEM CELLS INTERNATIONAL
(2022)
Article
Engineering, Biomedical
Saeed Ur Rahman, Woo-Jin Kim, Shin Hye Chung, Kyung Mi Woo
Summary: This study demonstrates that the fibrous topography of scaffolds can direct the differentiation of odontoblast-lineage cells to have the three-dimensional structure of odontoblasts. The altered responsiveness to Wnt5a on the nanofibrous substrate mediated the phosphorylation of JNK and polarity growth signaling, leading to the regeneration of tubular dentin with odontoblast processes. These findings suggest the potential application of nanofibrous scaffolds for dentin regeneration and provide evidence for the distinctive cell responsiveness to their micro-environments.
MATERIALS TODAY BIO
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hyang Lim, Ji-Su Oh, Kyeong-Rok Kang, Jeong-Yeon Seo, Do Kyung Kim, Sun-Kyoung Yu, Heung-Joong Kim, Joo-Cheol Park, Jae-Sung Kim
Summary: This study found that 25-HC can induce odontoclastogenesis in MDPC-23 cells by modulating the RANK-RANKL-OPG axis, potentially contributing to the pathophysiological processes of internal tooth resorption.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Juhyun Lee, Geumbit Hwang, Hyeri Gug, Ji-Hyun Lee, Su-Jin Park, Joo-Cheol Park
Summary: This study investigated the effects of toothpastes containing CPNE7-derived oligopeptide (CPNE7-DP) and other active desensitizing ingredients on dentin microleakage, tubule occlusion, and tertiary dentin formation. The results showed that toothpaste with CPNE7-DP had a long-term dentin sealing effect and promoted the formation of new hard tissue.
Review
Engineering, Biomedical
Woo Jin Kim, Young-Dan Cho, Young Ku, Hyun-Mo Ryoo
Summary: As the society ages, improving quality of life has become an important issue. With implants being accepted as a core component of oral rehabilitation treatment, competition to develop related technologies is intensifying. The analysis of 32,237 dental implant patents reveals advancements in technology, diverse geography, and a convergence towards combining various technological areas in the field. Dental implant technology has transitioned from conventional materials and surface treatments to new material technologies like zirconium and tantalum, as well as software technologies for diagnosis and prognosis. The growth of dental implant technology is expanding rapidly in East Asian countries, along with the growth of the Asian market. Overall, dental implant technology is developing by merging with different technological areas based on the local market environment.
BIOMATERIALS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jae- Moon, Woo-Jin Kim, Ki-Tae Kim, Hyun-Jung Kim, Hye-Rim Shin, Heein Yoon, Seung Gwa Park, Min-Sang Park, Young-Dan Cho, Pil-Jong Kim, Hyun-Mo Ryoo
Summary: An innovative automated image analysis method is presented in this study for precise quantification of subcellular foci within the cell nucleus. The method, called Foci-Xpress, is significantly faster and equally accurate compared to other conventional methods. Its automation eliminates biases caused by researchers and enhances experimental efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Heein Yoon, Seung Gwa Park, Hyun-Jung Kim, Hye-Rim Shin, Ki-Tae Kim, Young-Dan Cho, Jae- Moon, Min-Sang Park, Woo-Jin Kim, Hyun-Mo Ryoo
Summary: A study found that a form of vitamin B3 called nicotinamide (NAM) can prevent or treat bone diseases related to oxidative stress by increasing the production of antioxidant enzymes. NAM enhances mitochondrial function, increases the production of antioxidant enzymes, and promotes the formation of bone-building cells called osteoblasts. NAM increases mitochondrial biogenesis and antioxidant enzyme expression through activation of the SIRT3-FOXO3A axis, enhancing osteoblast differentiation.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Geumbit Hwang, Hyejin Seo, Joo-Cheol Park
Summary: Cpne7 deficiency leads to hepatic lipid metabolism abnormalities, excessive ROS production, and mitochondrial dysfunction. These findings are important for understanding the pathogenesis of NAFLD and suggest Cpne7 as a potential therapeutic target for NAFLD.