Article
Biochemistry & Molecular Biology
Qingzeng Gao, Jeremiah W. Jacob-Dolan, Rebecca A. Scheck
Summary: Advanced glycation end-products (AGEs) are irreversible protein modifications associated with aging and disease. Although the Parkinsonism-associated protein DJ-1 has been reported to possess deglycase activity, this study demonstrates that it is not a genuine deglycase and cannot remove AGEs. Instead, DJ-1 antagonizes glycation by detoxifying the glycating agent methylglyoxal (MGO) to lactate through its glyoxalase activity. The study also shows that DJ-1 is allosterically activated by glutathione.
Article
Biochemistry & Molecular Biology
Naouel Gharbi, Dagne Roise, Jorunn-Elise Forre, Amanda J. Edson, Helena A. Hushagen, Valentina Tronci, Ann-Kristin Froyset, Kari E. Fladmark
Summary: The redox-sensitive protein DJ-1 is essential in protecting the retina from oxidative stress, with its absence leading to age-dependent degenerative changes. Restoration of DJ-1 expression in Muller cells inhibits the degenerative phenotype, highlighting the key role of Muller cell DJ-1 in protecting the retina from oxidative stress associated with aging.
Article
Biochemistry & Molecular Biology
Sahil Gupta, Hajera Amatullah, James N. Tsoporis, Kuiru Wei, Ana Paula Teixeira Monteiro, Amin M. Ektesabi, Amir K. Varkouhi, Chirag M. Vaswani, Amanda Formosa, Alexandre T. Fabro, Sri Nagarjun Batchu, Chris Fjell, James A. Russell, Keith R. Walley, Andrew Advani, Thomas G. Parker, John C. Marshall, Patricia R. M. Rocco, Gregory D. Fairn, Tak Wah Mak, Claudia C. dos Santos
Summary: The ability to effectively clear infection is crucial for host survival. DJ-1 deficiency in bone marrow macrophages (BMM) promotes an increase in L3C-associated phagocytosis during bacterial infection, but not lipopolysaccharide infection. DJ-1 deficiency facilitates the complexing of Rubicon with Beclin-1 and UVRAG, leading to the assembly of autophagolysosomes decorated with LC3 molecules during intact bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Cell Biology
Judit Olah, Attila Lehotzky, Tibor Szenasi, Judit Ovadi
Summary: DJ-1, a multi-functional protein with antioxidant properties, protects dopaminergic neurons against Parkinson's disease by inhibiting the toxic assembly of SYN promoted by TPPP/p25. The interaction of DJ-1 with TPPP/p25 hinders the pathology of SYN with TPPP/p25, leading to decreased intracellular fluorescence and increased proteolytic degradation of SYN complexed with TPPP/p25. This study reveals a new protective function of DJ-1 against toxic SYN assemblies in living human cells.
Article
Biochemistry & Molecular Biology
Wenjuan Zhang, Bo Huang, Limo Gao, Cao Huang
Summary: Research has shown that a UBQLN2 mutation associated with ALS/FTD disrupts proteasome integrity in rats, leading to accumulation of proteasome subunits and impaired proteasome function. As the disease progresses and with increasing age, proteasome subunits are translocated to the nucleus, suggesting that defective proteasome function may result from mislocalization of subunits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Junpeng Mi, Xingping Zhou, Rou Sun, Jiaojiao Han
Summary: This study reveals that NT homodimers with sulfur substitution can form under alkaline conditions, explaining why intermolecular disulfide bonds have not evolved in the NT module during spider evolution of 380 million years. This discovery significantly advances our comprehension of spider evolution and silk spinning mechanisms, while also providing novel insights into protein storage, assembly, as well as the mechanisms and therapeutic strategies for neurodegenerative diseases associated with protein aggregation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Endocrinology & Metabolism
David E. Sleat, Whitney Banach-Petrosky, Katherine E. Larrimore, Yuliya Nemtsova, Jennifer A. Wiseman, Allison Najafi, Dymonn Johnson, Timothy A. Poole, Keigo Takahashi, Jonathan D. Cooper, Peter Lobel
Summary: Late-infantile neuronal ceroid lipofuscinosis (LINCL) and juvenile neuronal ceroid lipofuscinosis (JNCL) are inherited neurodegenerative diseases caused by mutations in the genes encoding lysosomal proteins TPP1 and CLN3, respectively. While enzyme replacement therapy has been successful for LINCL, effective treatments for JNCL are lacking due to unknown CLN3 protein function and limited robust animal models. Through creating a double mutant mouse model, this study found that the phenotype of the double mutant closely resembled that of the single TPP1 mutant, indicating potential use in JNCL therapy development. Additionally, analysis of brain proteomic changes in these models identified potential biomarker candidates for LINCL and altered lysosomal proteins in the Cln3 mutant animals.
JOURNAL OF INHERITED METABOLIC DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Shodai Suzuki, Satoshi Hiura, Taiki Mashiko, Takemi Matsumoto, Motoyuki Itoh
Summary: CADASIL is a genetic small vessel disease characterized by NOTCH3 mutation and abnormal aggregation of NOTCH3 mutant proteins. SD NOTCH3 mutants may be more likely to accumulate than SA NOTCH3 mutants upon interaction with JAG1, and LFNG may play an important role in promoting the aggregation of SA NOTCH3 mutants.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Hong Ki Min, Se Hee Kim, Ji-Yeon Lee, Sang-Heon Lee, Hae-Rim Kim
Summary: In this study, the effect of DJ-1 on helper T cell differentiation, fibroblast-like synoviocyte (FLS) activation, and osteoclastogenesis in rheumatoid arthritis was investigated. The results demonstrated that DJ-1 inhibits the production of pro-inflammatory cytokines and activation of RA-FLS, as well as osteoclast formation. Therefore, DJ-1 holds potential importance in the treatment of rheumatoid arthritis.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Ramani Shyam Kapuganti, Bushra Hayat, Biswajit Padhy, Pranjya Paramita Mohanty, Debasmita Pankaj Alone
Summary: This study investigates the role of DKK1 and ROCK2 in protein aggregation in PEX. The researchers found that DKK1 was upregulated in the lens capsule and conjunctiva tissues of PEX patients, along with an upregulation of ROCK2. Proteostat staining revealed increased protein aggregates in the lens epithelial cells of PEX patients. Overexpression of DKK1 led to increased protein aggregates and upregulation of ROCK2, while knockdown of DKK1 resulted in downregulation of ROCK2. Elevated levels of DKK1 were also observed in patients' plasma and aqueous humor. These findings suggest that DKK1 and ROCK2 might contribute to protein aggregation in PEX, and that elevated levels of DKK1 in aqueous humor can serve as a reliable classifier for pseudoexfoliation glaucoma.
Article
Cell Biology
Evelyne Naus, Marleen Derweduwe, Youlia Lampi, Annelies Claeys, Jarne Pauwels, Tobias Langenberg, Filip Claes, Jie Xu, Veerle Haemels, Zeynep Kalender Atak, Rob van der Kant, Joost Van Durme, Greet De Baets, Keith L. Ligon, Mark Fiers, Kris Gevaert, Stein Aerts, Frederic Rousseau, Joost Schymkowitz, Frederik De Smet
Summary: Induction of a heat shock-related stress response mediated by Foldlin reduces the levels of misfolded/aggregated mutant p53 and prevents the formation of stress-induced p53 nuclear inclusion bodies. The proteasome inhibitor Bortezomib has similar cellular effects as Foldlin. Induction of a cellular heat shock response seems to be an effective strategy to deal with pathological protein aggregation.
Article
Biochemistry & Molecular Biology
Tamr B. Atieh, Jonathan Roth, Xue Yang, Cody L. Hoop, Jean Baum
Summary: This study reveals the interaction mechanism between glycated alpha-synuclein and DJ-1, showing that DJ-1 can scavenge glycated alpha-synuclein oligomers, inhibit interactions between monomers and oligomers, and release free monomers. This mechanism helps suppress the propagation of toxic oligomers and fibril species.
Article
Cell Biology
Mohanad Ahmad, Josephine Esposto, Camilla Golec, Colin Wu, Sanela Martic-Milne
Summary: (English Summary:)
By systematically investigating the aggregation propensities of various gelsolin peptides and their mutants, researchers have identified G167R mutation as a promoter of aggregation compared to the wild-type sequence. The presence of inhibitors such as Methylene Blue (MB) and epigallocatechin gallate (EGCG) has been found to reduce gelsolin peptide aggregation, serving as both inhibitors of aggregation and promoters of disaggregation. This dual functionality of inhibitors presents a potential therapeutic strategy for preventing and reversing gelsolin amyloidosis, with implications for other amyloidogenic diseases of the heart, brain, and eye.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Anthony B. Schryvers
Summary: Gram-negative bacteria in the upper respiratory or genitourinary tracts of hosts rely on surface receptor proteins to acquire iron and proliferate independently. The diversity in these receptors supports the development of long-lasting vaccines.
TRENDS IN MICROBIOLOGY
(2022)
Article
Biology
Kailun Guo, Xue Feng, Weining Sun, Sirui Han, Shihua Wu, Haichun Gao
Summary: The DsbD protein is essential for cytochrome c maturation in bacteria. Using Shewanella oneidensis as a research model, it was found that the small subunit of nitrate reductase, NapB, can suppress the cytochrome c maturation defect caused by DsbD loss under anaerobic conditions. This study sheds light on the multifunctionality of cytochromes c and reveals a self-salvation strategy employed by bacteria to adapt to the natural environment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Fatma Guesmi, Linda Khantouche, Amel Mehrez, Houda Bellamine, Ahmed Landoulsi
HEART LUNG AND CIRCULATION
(2020)
Article
Biotechnology & Applied Microbiology
Yiqin Deng, Xing Luo, Mei Xie, Philippe Bouloc, Chang Chen, Annick Jacq
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Caroline Lacoux, Aymeric Fouquier D'Herouel, Francoise Wessner-Le Bohec, Nicolas Innocenti, Chantal Bohn, Sean P. Kennedy, Tatiana Rochat, Remy A. Bonnin, Pascale Serror, Erik Aurell, Philippe Bouloc, Francis Repoila
Article
Chemistry, Applied
Monia Bouktif Zarrouk, Esrine Gharbi, Imed Maatouk, Jean-Charles Leblanc, Ahmed Landoulsi
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2020)
Review
Biochemistry & Molecular Biology
Lionello Bossi, Nara Figueroa-Bossi, Philippe Bouloc, Marc Boudvillain
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2020)
Article
Biochemistry & Molecular Biology
Kim Boi Le Huyen, Cintia Daniela Gonzalez, Gaetan Pascreau, Valerie Bordeau, Vincent Cattoir, Wenfeng Liu, Philippe Bouloc, Brice Felden, Svetlana Chabelskaya
Summary: SprY, a novel small regulatory RNA carried by a prophage, titrates RNAIII activity and reduces the pathogenicity of Staphylococcus aureus through RNA sponge activity.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Xing Luo, Marick Esberard, Philippe Bouloc, Annick Jacq
Summary: In essence, the study demonstrates that the small RNA Vsr217 from Vibrio tasmaniensis promotes glycolysis and downregulates the expression of a key gluconeogenesis enzyme in the presence of maltose by regulating the positive regulator MalT. This aids the pathogen in adapting to the host environment during oyster infection.
Article
Microbiology
Marick Esberard, Marc Hallier, Wenfeng Liu, Claire Morvan, Lionello Bossi, Nara Figueroa-Bossi, Brice Felden, Philippe Bouloc
Summary: Bacterial small RNAs play important roles in regulating various pathways such as metabolism, virulence, and antibiotic resistance. The study reveals the critical involvement of 6S RNA in maintaining bacterial resistance to RNAP inhibitors and its protective effect is independent of σB activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
F. Repoila, F. Le Bohec, C. Guerin, C. Lacoux, S. Tiwari, A. K. Jaiswal, M. Passos Santana, S. P. Kennedy, B. Quinquis, D. Rainteau, V Juillard, S. Furlan, P. Bouloc, P. Nicolas, A. Miyoshi, V Azevedo, P. Serror
Summary: Enterococcus faecalis, a natural inhabitant of the human gastrointestinal tract, can become dominant and cause infections when the intestinal homeostasis is disrupted. The concentrations of bile acids deoxycholate and taurocholate could potentially impact the growth and population of E. faecalis.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Nissem Abdeljelil, Najla Ben Miloud Yahia, Ahmed Landoulsi, Abdelwaheb Chatti, Ruddy Wattiez, Rob Van Houdt, David Gillan
Summary: Bacteria biofilm formation and its complications are of special concern in isolated structures, and understanding this process and developing solutions are crucial for spacecraft efficiency and crew safety. In this study, interactions between Cupriavidus metallidurans and aerospace grade materials were investigated, revealing different biofilm formation patterns on different surfaces.
Article
Biochemistry & Molecular Biology
Rodrigo H. Coronel-Tellez, Mateusz Pospiech, Maxime Barrault, Wenfeng Liu, Valerie Bordeau, Christelle Vasnier, Brice Felden, Bruno Sargueil, Philippe Bouloc
Summary: Staphylococcus aureus adjusts its metabolism to cope with iron deprivation within the host, and the small non-coding RNA IsrR plays an important role in this process. IsrR down-regulates the translation of enzymes involved in anaerobic nitrate respiration during iron starvation. Additionally, IsrR is required for the full lethality of S. aureus in a mouse septicemia model.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Nara Figueroa-Bossi, Maria Antonia Sanchez-Romero, Patricia Kerboriou, Delphine Naquin, Clara Mendes, Philippe Bouloc, Josep Casadesus, Lionello Bossi
Summary: This study analyzed the mechanism of up-regulation of H-NS-silenced Salmonella pathogenicity island 1 (SPI-1) in cells depleted of Rho-cofactor NusG. It was found that when Rho cannot stop the transcription from spurious antisense promoters, it elongates into a H-NS-bound regulatory region of SPI-1, displacing H-NS and leading to the activation of SPI-1 transcription.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Dridi Imen, Haouel-Hamdi Soumaya, Cheraief Imed, Mediouni Ben Jemaa Jouda, Landoulsi Ahmed, Chaouch Rym
Summary: The study assessed the chemical composition, antioxidant, antimicrobial, and insecticidal activities of Lavandula dentata essential oil. Results indicated its potential value as a new antibacterial, antifungal, and bioinsecticide product.
JOURNAL OF ESSENTIAL OIL BEARING PLANTS
(2021)
Article
Biology
Wenfeng Liu, Pierre Boudry, Chantal Bohn, Philippe Bouloc
BMC RESEARCH NOTES
(2020)
Article
Environmental Sciences
Guesmi Fatma, Saidi Issam, Soussi Rawya, Hfaiedh Najla, Landoulsi Ahmed
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2019)