Article
Biology
Marie Barth, Alicia Toto Nienguesso, Anne Navarrete Santos, Carla Schmidt
Summary: This study quantifies the proteomes of SH-SY5Y cells during neuronal differentiation, revealing differences in protein expression under different conditions and identifying marker proteins for undifferentiated and differentiated cells. The study also explores protein-protein interactions within cells and observes structural reorganization of the actin cytoskeleton during differentiation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Zalesak-Kravec, Weiliang Huang, Jace W. Jones, Jianshi Yu, Jenna Alloush, Amy E. Defnet, Alexander R. Moise, Maureen A. Kane
Summary: This study investigated the impact of CRBP1 loss on the adult mouse heart using a multi-omics approach. The results showed that RA levels were decreased in Rbp1(-/-) mouse heart tissue, and metabolomics and proteomics analyses revealed disrupted pathways related to cellular metabolism and cardiac metabolism. These findings characterize the effect of CRBP1 loss and reduced RA in the adult heart.
Article
Biochemical Research Methods
Charles W. E. Tomlinson, Katy A. S. Cornish, Andrew Whiting, Ehmke Pohl
Summary: This article presents crystal structures of cellular retinoic acid-binding proteins CRABPI and CRABPII in complex with fatty acids and synthetic retinoids, shedding light on how these compounds influence retinoid signaling pathways. These co-crystal structures demonstrate canonical binding behavior and suggest potential therapeutic implications in serious diseases, such as neurodegenerative disorders.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Oncology
Kazuki Sakata, Mitsuro Kanda, Dai Shimizu, Shunsuke Nakamura, Yoshikuni Inokawa, Norifumi Hattori, Masamichi Hayashi, Chie Tanaka, Goro Nakayama, Yasuhiro Kodera
Summary: This study found that CRABP1 plays a promoting role in gastric cancer cells and may serve as a biomarker for predicting recurrence, especially peritoneal dissemination, after curative surgery.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Nutrition & Dietetics
King Clyde B. Yabut, Nina Isoherranen
Summary: Cellular retinoic acid binding proteins (CRABP1 and CRABP2) have a high affinity for all-trans-retinoic acid (atRA), and bind to atRA-clearing enzymes and nuclear receptors. This study shows that CRABP1 and CRABP2 also impact atRA metabolism by CYP26A1, suggesting their critical role in modulating atRA concentrations.
Article
Biochemistry & Molecular Biology
Maria Mesuraca, Clelia Nistico, Nicola Lombardo, Giovanna Lucia Piazzetta, Nadia Lobello, Emanuela Chiarella
Summary: Killian’s (antrochoanal) polyp is a unilateral nasal polypoid lesion that mainly affects children and young adults, with unclear etiology but potentially linked to chronic inflammation, autoimmune reactions, allergies, and viral infections. The study isolated and cultured mesenchymal stem cells from surgical biopsies of patients with this condition, revealing differences in cell proliferation, migration, and differentiation potential between KNP-MSCs and HNT-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Richard W. Carthew
Summary: Cell metabolism is closely connected to gene expression regulation, providing precursor molecules and ATP for gene expression. However, metabolic conditions are highly variable, requiring cells to continually calibrate gene expression to adapt to changing metabolite and energy budgets. Recent advances in understanding the molecular and biophysical mechanisms connecting metabolism and gene regulation, particularly in the context of organismal development, are discussed in this review.
TRENDS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Bo Dai, Feng Wang, Ying Wang, Jiayan Zhu, Yunxuan Li, Tingting Zhang, Luyao Zhao, Lining Wang, Wenhui Gao, Junmin Li, Honghu Zhu, Ke Li, Jiong Hu
Summary: Acute promyelocytic leukemia (APL) is driven by PML-RAR alpha oncoprotein, which is suppressed by HDAC3 inhibition to reduce the expression of PML-RAR alpha and induce differentiation, apoptosis, and decreased self-renewal of APL cells. HDAC3 deacetylates PML-RAR alpha, reducing its SUMOylation and subsequent ubiquitylation. Inhibiting HDAC3 with drugs or genetic methods shows promise as a strategy to treat relapsed/refractory APL.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Neurosciences
Charannya Sozheesvari Subhramanyam, Qiong Cao, Cheng Wang, Zealyn Shi-Lin Heng, Zhihong Zhou, Qidong Hu
Summary: piRNAs can regulate neuronal gene expression during neuronal differentiation and interact with specific RNA-binding proteins, providing new avenues for the treatment of neurodegenerative diseases.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Immunology
Chi-Ho Lee, David T. W. Lui, Karen S. L. Lam
Summary: Inflammation plays a crucial role in the pathogenesis of cardiovascular disease, with adipose tissue inflammation contributing to atherosclerosis. Adipocyte fatty acid-binding protein (AFABP) acts as a key mediator in the adipose-vascular cross-talk by interacting with JNK and AP-1, perpetuating inflammatory responses. Elevated circulating AFABP levels are associated with metabolic syndrome, type 2 diabetes, CVD, and other conditions related to inflammation and increased cardiovascular mortality.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Food Science & Technology
Bo Wang, Min Du
Summary: The rising prevalence of obesity is a serious public health threat. Vitamin A promotes adipose tissue vascular development, increases the number of adipose progenitors, and stimulates the browning process in white adipocytes as well as the thermogenic activity in brown/beige adipocytes. Hence, vitamin A is a promising anti-obesity micronutrient.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Multidisciplinary Sciences
Erin L. L. Sternburg, Jordan J. J. Lillibridge, Rattapol Phandthong, Fedor V. V. Karginov
Summary: Pumilio proteins PUM1 and PUM2 play a role in regulating cell morphology, migration, and adhesion. PDKO cells, lacking PUM proteins, exhibit a reduced collective cell migration rate, changes in cell morphology, and cell aggregation, which can be alleviated by the addition of extracellular matrix.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Robert R. Stickels, Evan Murray, Pawan Kumar, Jilong Li, Jamie L. Marshall, Daniela J. Di Bella, Paola Arlotta, Evan Z. Macosko, Fei Chen
Summary: Slide-seqV2 technology achieves an RNA capture efficiency close to 50% of single-cell RNA-seq data, which is 10 times higher than Slide-seq and approaches the detection efficiency of droplet-based single-cell RNA-seq techniques. Leveraging the high detection efficiency of Slide-seqV2, dendritically localized mRNAs in mouse hippocampal neurons were successfully identified, and spatial information was integrated with single-cell trajectory analysis tools to characterize the spatiotemporal development of the mouse neocortex, revealing underlying genetic programs that were poorly sampled with Slide-seq. The combination of near-cellular resolution and high transcript detection efficiency makes Slide-seqV2 applicable across various experimental contexts.
NATURE BIOTECHNOLOGY
(2021)
Article
Immunology
Yi Zhong, Sarah K. Walker, Yuri Pritykin, Christina S. Leslie, Alexander Y. Rudensky, Joris van der Veeken
Summary: Zhong et al. studied the transcriptional regulation of T cell gene expression upon viral challenge using B6/Cast F1 hybrid mice. They found that members of Ets, Runx, and TCF/Lef transcription factor families play key roles in maintaining or increasing chromatin accessibility during T cell activation. Some highly induced immune response genes exhibit a noncanonical TF recruitment pattern.
Article
Biochemical Research Methods
Hyeona Kang, Sun-Gu Lee
Summary: In this study, molecular dynamics simulations were performed to understand the differences in microscopic and dynamic behaviors between the non-binding and binding complexes of retinoic acid in cellular retinol-binding protein (CRBP). The results revealed the relative instability of the non-binding complex and showed very different dynamics and interactions of the ligand's terminal group. This study provides structural insights into the non-binding modes of retinoic acid in CRBP, which may have implications in retinoic acid-based drug discovery and protein engineering through computational modeling.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2023)