Article
Oncology
Junling Shen, Jilong Yang, Lei Sang, Rui Sun, Weiyu Bai, Chao Wang, Yan Sun, Jianwei Sun
Summary: In this study, elevated levels of p-PYK2 were found in vemurafenib-resistant melanoma cells, and PYK2 was implicated in regulating invadopodia formation. Inhibition of PYK2 reduced vemurafenib-induced invadopodia formation, and increased expressions of p-PYK2 in melanoma patients were associated with advanced stage, metastasis, and poor survival outcomes. These findings suggest that targeting PYK2 may resensitize melanoma cells to vemurafenib and that p-PYK2 could serve as a prognostic biomarker in melanoma.
CANCER BIOLOGY & MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sambit K. Nanda, Alan R. Prescott, Clara Figueras-Vadillo, Philip Cohen
Summary: The rapid formation and activation of the NLRP3 inflammasome is induced by co-stimulation with LPS and nigericin. IKK beta plays a crucial role in recruiting NLRP3 to the dispersed TGN, leading to the formation and activation of the NLRP3 inflammasome.
Article
Biochemistry & Molecular Biology
America Chandia-Cristi, Nicolas Stuardo, Cristian Trejos, Nancy Leal, Daniela Urrutia, Francisca C. Bronfman, Alejandra Alvarez Rojas
Summary: Brain-derived neurotrophic factor (BDNF) activates TrkB receptor and downstream pathways, promoting neuronal survival, growth, and plasticity. The role of c-Abl, a non-receptor tyrosine kinase, in BDNF-induced dendritic arborization was examined using various strategies. Surprisingly, BDNF promoted c-Abl activation and interaction with TrkB receptors. Inhibition or genetic ablation of c-Abl abolished BDNF-induced dendritic arborization without affecting classic TrkB downstream pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Oncology
Sergei Kusmartsev
Summary: The study demonstrates a positive association between AKI induced by ischemia-reperfusion injury and the formation of clear-cell renal cell carcinoma in genetically modified mice, with blockade of CXCL1 signaling inhibiting kidney tumor emergence. This provides a new experimental approach to prevent and reduce kidney cancer incidence through modulation of the AKI-induced inflammatory response.
Article
Cell Biology
Dylan Jones, Clarissa A. Whitehead, Marija Dinevska, Samuel S. Widodo, Liam M. Furst, Andrew P. Morokoff, Andrew H. Kaye, Katharine J. Drummond, Theo Mantamadiotis, Stanley S. Stylli
Summary: Glioblastoma is a highly invasive and destructive brain tumor, and current standard treatments often fail to effectively eliminate it. In this study, we investigate the effects of several FDA-approved drugs on the invasion and viability of GBM cells. We found that these drugs can reduce cell invasion and viability, suggesting their potential as therapeutic options for GBM.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Cell Biology
Sandra Abdellatef, Isabelle Fakhoury, Maria Al Haddad, Leila Jaafar, Hiba Maalouf, Samer Hanna, Bassem Khalil, Zeinab El Masri, Louis Hodgson, Mirvat El-Sibai
Summary: The study reveals the potential role of Cdc42 GAP StarD13 in modulating cell motility and invasion in ovarian cancer cells, inhibiting the invasion process. StarD13 and Cdc42 activation appear to be mutually exclusive in invadopodial structures, with Cdc42 potentially playing a direct role in recruiting TKS4 to invadopodia. These findings suggest the suitability of StarD13 for targeted therapy in ovarian cancer.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Takuma Matsubara, William N. Addison, Shoichiro Kokabu, Lynn Neff, William Horne, Francesca Gori, Roland Baron
Summary: Deletion of c-Src results in osteoclast dysfunction and osteopetrosis, which cannot be compensated for by other SFKs. The unique function of c-Src in osteoclasts is partially derived from its interaction with c-Cbl, crucial for cytoskeleton organization and efficient bone resorption.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Marwah Karim, Sirle Saul, Luca Ghita, Malaya Kumar Sahoo, Chengjin Ye, Nishank Bhalla, Chieh-Wen Lo, Jing Jin, Jun-Gyu Park, Belen Martinez-Gualda, Michael Patrick East, Gary L. Johnson, Benjamin A. Pinsky, Luis Martinez-Sobrido, Christopher R. M. Asquith, Aarthi Narayanan, Steven De Jonghe, Shirit Einav
Summary: This study investigates the functional relevance of NAKs in SARS-CoV-2 infection and demonstrates that pharmacological inhibition of NAKs can be a potential approach to treat COVID-19.
ANTIVIRAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Kyung Ah Kim, Srivithya Vellampatti, Sun Ha Hwang, Byoung Choul Kim
Summary: This study developed a new DNA-based force probe for investigating the effect of integrin tension on the formation and maturation of invadopodia. The research found that integrin tension above 40 pN is critical for the maturation of invadopodia, eventually leading to the generation of a strong invadopodial force greater than 100 pN. These findings provide new insights into the biophysical mechanism underlying cancer invasion and metastasis.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Thomas Juan, Agatha Ribeiro da Silva, Barbara Cardoso, SoEun Lim, Violette Charteau, Didier Y. R. Stainier
Summary: Cardiac valves play a vital role in ensuring unidirectional blood flow through the heart. Researchers have identified several mechanosensory genes as key regulators of valve development, expanding our understanding of this process.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hui Wang, Friedemann Zaiser, Priska Eckert, Johannes Ruf, Nicolas Kayser, Anna C. Veenstra, Merle Mueller, Rebecca Haas, Gerd Walz, Toma A. Yakulov
Summary: Nephronophthisis (NPH), an autosomal recessive ciliopathy, is caused by mutations in more than 20 different genes (NPHPs). These mutations affect protein complexes involved in cilium trafficking and developmental processes. In this study, the zebrafish mutant line invssa36157 was used to investigate the tissue-specific functions of zebrafish Nphp2. The results showed that the invssa36157 mutants exhibited mild ciliopathy phenotypes, increased glomerular and cloaca cyst formation, and enhanced susceptibility to the depletion of the nphp1/nphp2/nphp8 module. The simultaneous knockdown of zebrafish nphp1 and vangl2 led to pronounced cloaca malformations in the invssa36157 mutant embryos, highlighting the role of these genes in normal cloaca formation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Claire Marquilly, Germain U. Busto, Brittany S. Leger, Ana Boulanger, Edward Giniger, James A. Walker, Lee G. Fradkin, Jean-Maurice Dura
Summary: The study reveals that Htt and Appl in Drosophila interact antagonistically, with Htt acting as an inhibitor of Abl tyrosine kinase activity, while Appl promotes it, contributing to the balance in promoting axon growth.
Article
Oncology
Baolin Wang, Bowen Zheng, Li Cao, Kexi Liao, Deng Huang, Yujun Zhang, Yan Jiang, Shuguo Zheng
Summary: Tiam1 plays a crucial role in the invasion and metastasis of hepatocellular carcinoma by promoting invadopodia formation through Rac1 activation, regulated by the PI3K/Akt signaling pathway and facilitated by PIP2.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Oncology
Xiao-Ming Liu, Fang Chen, Li Wang, Fan Zhang, Li-Jun Huo
Summary: In this study, the joint roles of Chk1/2 in oocyte meiotic maturation were examined, with Chk1/2 inhibition resulting in defective meiotic spindle organization and chromosome condensation. Interestingly, inhibiting Chk1 alone had different effects compared to Chk1/2 inhibition, indicating distinct functions of Chk1 and Chk2 during oocyte maturation. These findings provide further insights into the functions and regulatory mechanisms of Chk1/2 in oocyte meiotic maturation.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Li-Qin Hu, Shi-Xia Yu, Wan-Yue Xu, Song-Hao Zu, Yu-Tong Jiang, Hao-Tian Shi, Yan-Jie Zhang, Hong-Wei Xue, Ying-Xiang Wang, Wen-Hui Lin
Summary: In Arabidopsis thaliana, the development of female gametophyte (FG) involves the formation and expansion of large vacuoles. The VACUOLELESS GAMETOPHYTES (VLG) gene is responsible for facilitating the fusion of vesicles to form these vacuoles, but its regulation is still not well understood. This study found that mutations in the BRASSINOSTEROID INSENSITIVE2 (BIN2) gene increased the abundance of VLG, leading to excessive vacuole formation and FG abortion. On the other hand, mutations in BIN2 and its homologs reduced VLG abundance and caused phenotypes similar to vlg/VLG mutants. It was also discovered that BIN2 interacts with and phosphorylates VLG, enhancing its stability and abundance to promote vacuole formation.
Article
Biochemistry & Molecular Biology
Paul C. Trampont, Li Zhang, Amber J. Giles, Scott F. Walk, Jing J. Gu, Ann Marie Pendergast, Kodi S. Ravichandran
MOLECULAR AND CELLULAR BIOLOGY
(2015)
Article
Cell Biology
Aaditya Khatri, Jun Wang, Ann Marie Pendergast
JOURNAL OF CELL SCIENCE
(2016)
Article
Medicine, Research & Experimental
Yoshinori Matsumoto, Jose La Rose, Oliver A. Kent, Melany J. Wagner, Masahiro Narimatsu, Aaron D. Levy, Mitchell H. Omar, Jiefei Tong, Jonathan R. Krieger, Emily Riggs, Yaryna Storozhuk, Julia Pasquale, Manuela Ventura, Behzad Yeganeh, Martin Post, Michael F. Moran, Marc D. Grynpas, Jeffrey L. Wrana, Giulio Superti-Furga, Anthony J. Koleske, Ann Marie Pendergast, Robert Rottapel
JOURNAL OF CLINICAL INVESTIGATION
(2016)
Article
Biochemistry & Molecular Biology
Jun Wang, Clay Rouse, Jeff S. Jasper, Ann Marie Pendergast
Article
Biochemistry & Molecular Biology
Jun Wang, Clay Rouse, Jeff S. Jasper, Ann Marie Pendergast
Article
Multidisciplinary Sciences
Aaditya Khatri, Bryan D. Kraft, Purushothama Rao Tata, Scott H. Randell, Claude A. Piantadosi, Ann Marie Pendergast
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Oncology
Peter E. Fecci, Cosette D. Champion, Jacob Hoj, Courtney M. McKernan, C. Rory Goodwin, John P. Kirkpatrick, Carey K. Anders, Ann Marie Pendergast, John H. Sampson
CLINICAL CANCER RESEARCH
(2019)
Article
Cell Biology
Jacob P. Hoj, Benjamin Mayro, Ann Marie Pendergast
Article
Multidisciplinary Sciences
Jacob P. Hoj, Benjamin Mayro, Ann Marie Pendergast
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Cell Biology
Jillian Hattaway Luttman, Ashley Colemon, Benjamin Mayro, Ann Marie Pendergast
Summary: ABL kinases are involved in promoting tumor progression and metastasis in solid tumors, with their inhibition potentially serving as a novel treatment approach for advanced metastatic disease.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Jillian Hattaway Luttman, Jacob P. Hoj, Kevin H. Lin, Jiaxing Lin, Jing Jin Gu, Clay Rouse, Amanda G. Nichols, Nancie J. MacIver, Kris C. Wood, Ann Marie Pendergast
Summary: Targeting mitochondrial metabolism and the mevalonate pathway through ABL tyrosine kinase inhibition and statin therapy can synergistically decrease the survival of metastatic lung cancer cells, leading to decreased metastatic outgrowth and increased survival rates for patients with advanced lung cancer.
Article
Cell Biology
Courtney M. McKernan, Aaditya Khatri, Molly Hannigan, Jessica Child, Qiang Chen, Benjamin Mayro, David Snyder, Christopher V. Nicchitta, Ann Marie Pendergast
Summary: The study found that ABL kinase allosteric inhibitors can improve overall survival and impair brain metastasis in patients with HER2-positive breast cancer. Mechanistically, ABL kinase phosphorylates Y-box-binding protein 1 (YB-1), disrupting its binding to the HER2 gene and leading to a decrease in HER2 protein levels. Loss of YB-1 also inhibits the development of brain metastasis and impairs the expression of ABL-dependent brain metastatic targets.
Review
Oncology
Hannah L. Thrash, Ann Marie Pendergast
Summary: YAP and TAZ, as transcriptional co-activators in the Hippo pathway, play important roles in tumor progression and metastasis. This review provides insights into their structure, regulation, and their roles in tumor invasion and metastatic spread. It also discusses potential therapeutic strategies targeting YAP/TAZ in different types of solid tumors.
Article
Multidisciplinary Sciences
Jing Jin Gu, Jacob Hoj, Clay Rouse, Ann Marie Pendergast
Review
Oncology
Jun Wang, Ann Marie Pendergast