Article
Biology
Norbert Minet, Anne-Claire Boschat, Rebecca Lane, David Laughton, Philip Beer, Helene Asnagli, Claire Soudais, Tim Bourne, Alain Fischer, Emmanuel Martin, Sylvain Latour
Summary: CTP nucleotide is essential for DNA replication. Both CTPS1 and CTPS2 play roles in cell proliferation, but CTPS1 is more efficient and contributes more to cell growth. CTPS2 becomes essential in the absence of CTPS1. The analysis of cancer cell lines confirms the importance of CTPS1 in cell growth.
LIFE SCIENCE ALLIANCE
(2023)
Article
Cell Biology
Barbara Samper-Martin, Ana Sarrias, Blanca Lazaro, Marta Perez-Montero, Rosalia Rodriguez-Rodriguez, Mariana P. C. Ribeiro, Aitor Banon, Don Wolfgeher, Henning J. Jessen, Berta Alsina, Josep Clotet, Stephen J. Kron, Adolfo Saiardi, Javier Jimenez, Samuel Bru
Summary: Polyphosphate (polyP) is a polymer of phosphate residues found in all organisms, and in mammals it plays a crucial role in physiological processes. Despite decades of research, the metabolic enzymes responsible for polyP were unknown until Nudt3 was identified as the enzyme in mammalian cells. Nudt3 shows specificity for polyP in the presence of Zn2+ and is involved in oxidative stress protection and DNA damage repair.
Article
Microbiology
Tania Jorda, Nicolas Rozes, Sergi Puig
Summary: Iron deficiency affects iron uptake and growth in yeast cells, while changes in ergosterol biosynthesis patterns impact the response to iron deprivation. Mislocalization of the iron-sensing factor Aft1 to the vacuole disrupts its transcriptional activation function. These findings reveal novel connections between iron and sterol metabolisms with implications for yeast-derived food production and treatment of fungal infections with ergosterol biosynthesis-targeting drugs.
Article
Microbiology
Buli Su, Anzhang Li, Ming-Rong Deng, Honghui Zhu
Summary: Nutritional components significantly impact carotenoid accumulation in engineered Saccharomyces cerevisiae, with copper ions playing a prominent role in increasing carotenoid yield. Transcriptome analysis revealed key genes like ADY2, HES1, and CUP1, and overexpression of ACE1 in YPD media led to a 6.4-fold increase in carotenoid yield. This study provides insights for genetic engineering strategies to enhance carotenoid production in yeast.
Review
Microbiology
Antonia Maria Romero, Maria Teresa Martinez-Pastor, Sergi Puig
Summary: Iron is essential for eukaryotic translation, affecting translation initiation, specific transcript translation repression, and various details of the translation process through the action of specific regulators. The influence of iron on different stages of translation and its correlation with protein biosynthesis control are key areas of research interest.
Article
Biotechnology & Applied Microbiology
Wenyan Cao, Weiquan Zhao, Bolun Yang, Xinning Wang, Yu Shen, Tiandi Wei, Wensheng Qin, Zailu Li, Xiaoming Bao
Summary: Vanillin is an important phenolic inhibitor in Saccharomyces cerevisiae, inhibiting the translation process. Deletion of YRR1 enhances vanillin resistance by promoting translation-related processes. Proteomic changes induced by vanillin stress and YRR1 deletion provide insights into yeast's protective response to vanillin.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Ana Gaspar-Cordeiro, Catarina Amaral, Vania Pobre, Wilson Antunes, Ana Petronilho, Paulo Paixao, Antonio P. Matos, Catarina Pimentel
Summary: The combination of copper and fluconazole has synergistic effects against resistance Candida glabrata. Copper affects the efflux of fluconazole and the biosynthesis of ergosterol, leading to the accumulation of toxic sterols. It also affects protein function in the plasma membrane, causing structural alterations in cells. The combination treatment downregulates gene expression and induces zinc depletion, which contributes to the observed synergistic effect.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
R. Purshotham Naik, V. Chinni Preetam, N. Nalini Kumari, M. V. L. N. Raju, B. Prakash, M. R. Reddy
Summary: The research found that at appropriate zinc concentrations, yeast growth can be promoted, but higher zinc concentrations have a significant negative impact on yeast biomass yield. Zinc sulfate is the most effective source for increasing zinc content in yeast cells.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Alexandr A. Tomashevsky, Valery V. Petrov
Summary: Both ATP and inorganic polyphosphates are involved in yeast energy homeostasis, with the PMA1 H+-ATPase playing a key role. The structure and phosphorylation of the enzyme affect its function and its relationship with PolyP metabolism. Substitutions of specific residues in different domains of the enzyme have varied effects on PolyP content and distribution, depending on the type of substitution and its impact on ATPase activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Food Science & Technology
Gang Du, Xingyu Zhang, Yuting Gao, Cunying Sun, Liwen Wang, Wei Zhao, Dan Meng, Wenqiang Guan, Hui Zhao
Summary: Ginger is commonly used in traditional Chinese medicine as a natural remedy for cold, and it has thermogenic effects when consumed. This study revealed that ginger oleoresin stress and heat stress had similar effects on yeast and shared genes. The transcription factor Hsf1 was found to be phosphorylated and activated in response to ginger oleoresin stress, leading to increased expression of heat shock proteins.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Stephen R. Hennigar, Cassandra Olson, Alyssa M. Kelley, James P. McClung
Summary: This study investigated the expression of zinc transporters in the small intestine of mice and found that Slc39a4 is positively associated with the appearance of Zn-67 in the liver and plasma. Most zinc transporters are expressed in the small intestine, but Slc39a4 predicts fractional zinc absorption and utilization in young mice.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiang Meng, Alan Reed, Sandie Lai, Juraj Szavits-Nossan, John E. G. McCarthy
Summary: Gene expression stochasticity is an inherent feature of biological systems, creating non-genetic cellular variation and influencing various processes. In this study, the researchers discovered a distinct form of non-transcriptional noise associated with the translation machinery and mRNA 5'UTR of the GCN4 gene in yeast. They characterized the heterogeneity of translation initiation mediated by GCN4-5'UTR using different techniques and found a subpopulation of cells that consistently exhibited enhanced GCN4 translation under non-starvation conditions.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Emilios Tassios, Christoforos Nikolaou, Nikolaos Vakirlis
Summary: Intergenic genomic regions have important regulatory and structural roles, and can potentially encode transmembrane domains. This property is found in intergenic regions of the yeast genome and is associated with the presence of transmembrane domains in evolutionarily young genes.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Cell Biology
Rasha Khaddaj, Jiri Stribny, Stephanie Cottier, Roger Schneiter
Summary: Lipid droplets (LDs) form and associate closely with the endoplasmic reticulum (ER) to store energy-rich lipids. Membrane-anchored perilipins induce expansion and vesiculation of the perinuclear membrane, forming crescent-shaped membrane domains with LD-like properties. These domains are enriched in diacylglycerol (DAG) and ER proteins important for LD biogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Xingyue Li, Lin Zhang, Haiyan Ren, Xiaoyu Wang, Fugui Mi
Summary: The study found that high levels of zinc supply can reduce plant biomass and chlorophyll content, and interfere with photosynthesis in steppe plants. Additionally, high zinc supply can lead to growth defects and changes in ion concentrations. However, low levels of zinc supply do not affect plant growth performance. A novel protein, CaMTP, was also identified through RNA transcriptome analysis, which may be a potential candidate for overcoming mineral stress in dicot crop plants.
FRONTIERS IN PLANT SCIENCE
(2022)