Article
Multidisciplinary Sciences
Maria Dzamukova, Tobias M. Brunner, Jadwiga Miotla-Zarebska, Frederik Heinrich, Laura Brylka, Mir-Farzin Mashreghi, Anjali Kusumbe, Ralf Kuehn, Thorsten Schinke, Tonia L. Vincent, Max Loehning
Summary: The study reveals that mechanical forces trigger the secretion of dentin matrix protein 1 from osteoblasts, leading to the transformation of bone growth-promoting blood vessels into a quiescent subtype, thus limiting bone growth at the end of adolescence.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
F. Machla, V Sokolova, V Platania, O. Prymak, K. Kostka, B. Kruse, M. Agrymakis, S. Pasadaki, A. Kritis, K. Alpantaki, M. Vidaki, M. Chatzinikolaidou, M. Epple, A. Bakopoulou
Summary: Tissue engineering provides new treatment approaches for regenerating lost dental tissues, and this study investigated the characteristics of EDTA-treated, freeze-dried dentin matrices (HTFD scaffolds) conditioned with calcium phosphate nanoparticles (NPs) bearing dentinogenesis-inducing factors. The synthesized NPs showed efficient transfection of dental pulp stem cells (DPSCs), and HTFD/NPs constructs induced odontogenic shift of DPSCs, preserving cell viability and leading to the reconstruction of the ortho-dentin/odontoblastic layer barrier.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Medicinal
Luke A. Adams, Lorna E. Wilkinson-White, Menachem J. Gunzburg, Stephen J. Headey, Biswaranjan Mohanty, Martin J. Scanlon, Ben Capuano, Joel P. Mackay, Bradley C. Doak
Summary: A systematic workflow, called Rapid Elaboration of Fragments into Leads (REFiL), allows for the rapid improvement of binding affinity and evolution of low-affinity fragment hits into higher-affinity leads without the need for structural information.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
S. Pragnere, J-C Auregan, C. Bosser, A. Linglart, M. Bensidhoum, T. Hoc, C. Nouguier-Lehon, C. Chaussain
Summary: Osteogenesis imperfecta is a rare genetic disorder of the skeleton, with research showing an increase in dentin hardness and mineral content in patients with mild form of the disease, despite about half of patients showing no obvious oral manifestations.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Physical
Maiko Yamada, Motohiko Nagayama, Yuka Miyamoto, Satoshi Kawano, Yoshiaki Takitani, Masashi Tanaka, Michiko Ehara, Juna Nakao, Takanaga Ochiai, Yoshihiro Shibukawa, Takakazu Yoshida
Summary: MTA can promote early differentiation of IPMSCs into odontoblasts, while MTA combined with PRP can stimulate calcified granule and dentin bridge formation through calcium mineral deposition. This combination is an effective method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration.
Article
Cell Biology
Ziyi Liu, Xinfeng Bai, Peifeng Wan, Fan Mo, Ge Chen, Jian Zhang, Jiangang Gao
Summary: Research shows that the lack of Loxl3 in the inner ear may lead to hearing loss, hair cell degeneration, and neuronal degeneration, indicating that Loxl3 plays a crucial role in maintaining auditory function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Materials Science, Biomaterials
Runying Guo, Rui Zhang, Sirui Liu, Yanyu Yang, Wenhang Dong, Meiyue Wang, Hongyan Mi, Mengzhe Liu, Jingjing Sun, Xue Zhang, Yimeng Su, Yiming Liu, Di Huang, Rui Li
Summary: A new composite material composed of treated dentin matrix (TDM) and alpha-calcium sulphate hemihydrate (alpha-CSH) was successfully prepared and the optimal composition ratio was explored. The results showed that the TDM/alpha-CSH composite had good physical mixing and structural stability. It exhibited comparable compressive strength to human cancellous bone tissues and excellent biocompatibility with increased expression of osteogenic related genes and proteins. In vivo experiments demonstrated that TDM effectively promoted cell proliferation and osteomalacia. The 50% TDM/alpha-CSH combination displayed optimal osteoconductivity, making it a promising candidate for bone tissue engineering applications.
REGENERATIVE BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Lijie Mao, Yanrong Yin, Lixin Zhang, Xiaolei Chen, Xinqing Wang, Fangping Chen, Changsheng Liu
Summary: Neutralizing acidity to improve cell proliferation and bone repair is a promising strategy. In this study, a polymer containing an alkaline fragment was synthesized and used to fabricate porous scaffolds, effectively neutralizing acidity and improving biocompatibility and cell adhesion and proliferation. The scaffolds also reduced inflammatory response and promoted bone cell regeneration.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jeremie Silvent, Marc Robin, Camila Bussola Tovani, Yan Wang, Fabrice Soncin, Sidney Delgado, Thierry Azais, Capucine Sassoye, Marie-Madeleine Giraud-Guille, Jean-Yves Sire, Nadine Nassif
Summary: The confinement of collagen suprafibrillar in bone plays a crucial role in the activity of biomimetic acidic calcium-binding polymers on apatite mineralization. The specificity of the 57-KD fragment of DMP1 in the regulation of mineralization is validated without phosphorylation. Transient apatite phases are only found using a high nonphysiological concentration of additives.
Article
Biochemistry & Molecular Biology
Jinqiang Guo, Shuaitong Yu, Hanshu Zhang, Lu Zhang, Guohua Yuan, Huan Liu, Zhi Chen
Summary: This study found that mice with KLF4 deficiency had underdeveloped mandibles and decreased alveolar bone mass. The reduced expression of osteoblast-related genes and attenuated osteoclastogenesis were also observed in these mice. The findings suggest that KLF4 plays a critical role in mandibular development.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yixin Xu, Yixin Wang, Hui Xiao, Yongming Li
Summary: This research elucidated the molecular mechanisms underlying periodontitis-associated bone loss, specifically focusing on the role of the hypoxic microenvironment. The findings revealed that hypoxia dissociates the FOXO1-HDAC6 complex and aggregates them in the nucleus, regulating autophagy levels and bone metabolism.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyan Chen, Nan Li, Ping Hu, Leilei Li, Danya Li, Han Liu, Lei Zhu, Jing Xiao, Chao Liu
Summary: Cleft palate is a common birth defect, and this study focused on the role of glycosaminoglycan (GAG) chains in palate development. The reduction of GAG chains led to increased apoptosis, decreased cell density, and decreased palatal volume. This study also revealed impaired osteogenesis of the palatine, which could be partially rescued by activation of Bmpr1a.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ashwini Kumar Nepal, Hubertus W. van Essen, Christianne M. A. Reijnders, Paul Lips, Nathalie Bravenboer
Summary: Mechanical loading of bone affects factors of phosphate homeostasis by modulating the gene expression of Fgf23, Mepe, Dmp1, Phex, Cyp27b1, and Vdr. It results in a decrease in Fgf23 gene expression and serum FGF23 levels, as well as an increase in Dmp1 and Mepe gene expression. However, it does not affect the gene expression of Phex, Cyp27b1, and Vdr.
Article
Biotechnology & Applied Microbiology
Ilia Y. Bozo, Alexey Y. Drobyshev, Nikolay A. Redko, Vladimir S. Komlev, Artur A. Isaev, Roman Deev
Summary: This study developed a gene-activated bone substitute, which was proven feasible, safe, and effective for jaw bone grafting in both animal models and clinical trials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Mariana Reis, Yvette Alania, Ariene Leme-Kraus, Robert Free, Derk Joester, Weikang Ma, Thomas Irving, Ana K. Bedran-Russo
Summary: Aging has a profound impact on the biology and function of human teeth. The stiffness of root dentin increases with age, but its ability to dissipate energy during deformation does not decay. The damping factor of the organic matrix deteriorates with age. Altered matrix-mineral interactions and changes in glycosaminoglycans may counteract the negative effects of aging on the structural components of the extracellular matrix.
ACTA BIOMATERIALIA
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shuang Zhou, Gongchen Li, Tingting Zhou, Shuai Zhang, Hui Xue, Jiangyu Geng, Wenjing Liu, Yao Sun
Summary: The study found that ciliary intraflagellar transporter protein 140 (IFT140) plays a positive role in promoting extraction socket healing. Compared to the control group, the experimental group with reduced IFT140 levels showed delayed healing and decreased proliferation and osteogenic differentiation ability of alveolar bone marrow stromal cells (aBMSCs).
Article
Dentistry, Oral Surgery & Medicine
J. Li, F. Jin, M. Cai, T. Lin, X. Wang, Y. Sun
Summary: Periodontitis is a common chronic oral disease characterized by bone resorption. The study found that the lncRNA Nron can inhibit osteoclast activity and bone resorption, showing potential for new treatment strategies.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Fujun Jin, Junhui Li, Yong-Biao Zhang, Xiangning Liu, Mingxiang Cai, Meijing Liu, Mengyao Li, Cui Ma, Rui Yue, Yexuan Zhu, Renfa Lai, Zuolin Wang, Xunming Ji, Huawei Wei, Jun Dong, Zhiduo Liu, Yifei Wang, Yao Sun, Xiaogang Wang
Summary: Long noncoding RNA Nron has been identified as a key suppressor of bone resorption, and delivery of its functional motif to osteoclasts can potentially increase bone mass in mouse models, offering a treatment option with less risk of side effects.
NATURE COMMUNICATIONS
(2021)
Article
Anatomy & Morphology
Kaoru Fujikawa, Naoko Nonaka, Xiaofang Wang, Shunichi Shibata
Summary: The expression patterns of syndecan-1, 2, 3, and 4 mRNAs during late tooth germ formation were investigated. Syndecan-1 mRNA was mainly expressed in specific tooth germ regions and its expression in dental epithelium increased during the postnatal periods. Syndecan-3 mRNA was found to be involved in dentinogenesis, while syndecan-2 showed enhanced expression with hard tissue formation. Syndecan-4 exhibited similar expression patterns to syndecan-3.
ANATOMICAL SCIENCE INTERNATIONAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xuyan Gong, Han Zhang, Xiaoqiao Xu, Yunpeng Ding, Xingbo Yang, Zhiyang Cheng, Dike Tao, Congjiao Hu, Yaozu Xiang, Yao Sun
Summary: The study reveals the significant role of PRX1(+) cells in tooth and periodontal ligament (PDL) development. They are involved in the formation of mouse molars and PDL, and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Editorial Material
Dentistry, Oral Surgery & Medicine
Judith Albino, Bruce A. Dye, Rena N. D'Souza
JOURNAL OF DENTAL EDUCATION
(2022)
Article
Cell Biology
Xin Fang, Nan Ni, Xiaofang Wang, Yanan Tian, Ivan Ivanov, Monique Rijnkels, Kayla J. Bayless, John P. Lydon, Qinglei Li
Summary: EZH2 has dual roles in the development of endometrial cancer, functioning as an oncogene in the early stages but also leading to unfavorable outcomes during disease progression, characterized by exacerbated epithelial stratification and heightened inflammatory response.
Article
Biochemistry & Molecular Biology
Changchun Dong, Bikash Lamichhane, Hajime Yamazaki, Brent Vasquez, Jingya Wang, Yongxu Zhang, Jian Q. Feng, Henry C. Margolis, Elia Beniash, Xiaofang Wang
Summary: This study generated two lines of Enam knock-in mice using the CRISPR-Cas9 method to eliminate or mimic the phosphorylation state of Ser(55) in ENAM. The results showed that Ser(55) phosphorylation is essential for ENAM function during enamel formation.
Article
Biology
Bhaba K. Das, Lei Wang, Toshifumi Fujiwara, Jian Zhou, Nukhet Aykin-Burns, Kimberly J. Krager, Renny Lan, Samuel G. Mackintosh, Ricky Edmondson, Michael L. Jennings, Xiaofang Wang, Jian Q. Feng, Tomasa Barrientos, Jyoti Gogoi, Aarthi Kannan, Ling Gao, Weirong Xing, Subburaman Mohan, Haibo Zhao
Summary: Increased intracellular iron through Tfr1-mediated iron uptake is essential for mitochondrial metabolism and cytoskeletal organization in osteoclasts, leading to proper bone resorption and skeletal homeostasis.
Article
Multidisciplinary Sciences
Changchun Dong, Bikash Lamichhane, Yongxu Zhang, Xiaofang Wang
Summary: This study describes a novel method for visualizing and analyzing label-retaining cells (LRCs) in mouse incisors. By using tissue clearing and whole-mount EdU staining, combined with confocal microscopy and imaging software for 3D reconstruction, this method enables unbiased quantitation compared to traditional LRCs analysis on sectioned slides.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xueming Zhang, Ji Zhou, Xinyu Wang, Jiangyu Geng, Yubei Chen, Yao Sun
Summary: Stem/progenitor cells play a crucial role in salivary gland development, homeostasis, and regeneration. This study identified IFT140(+)/K14(+) as a combined marker for salivary gland stem/progenitor cells and elucidated the essential role of IFT140 and cilia in regulating cell differentiation and gland regeneration.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zheng Wang, Chi Ma, Diane Chen, Caitlin Haslett, Chunmei Xu, Changchun Dong, Xiaofang Wang, Minghao Zheng, Yan Jing, Jian Q. Feng
Summary: This study reveals that tendon cells directly form fibrocartilage through cell transdifferentiation, shedding light on the mechanisms underlying enthesopathy and sporting injuries.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jinyuan Hu, Junhui Li, Jennifer Jiang, Lingling Wang, Jonathan Roth, Kenneth N. McGuinness, Jean Baum, Wei Dai, Yao Sun, Vikas Nanda, Fei Xu
Summary: Collagens are essential for mediating cell-matrix interactions and tissue mechanics. Synthetic collagens that mimic the structure and properties of natural collagen have been developed, and they could serve as safe and functional biomaterials for various applications.
NATURE COMMUNICATIONS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Han Zhang, Xuyan Gong, Xiaoqiao Xu, Xiaogang Wang, Yao Sun
Summary: Tooth number abnormality, including tooth agenesis and supernumerary teeth, is a common dental developmental disease. The regulation of tooth development involves various developmental signals, such as Wnt, BMP, FGF, Shh, and Eda pathways, which mediate the interactions between epithelium and mesenchyme. This review summarizes the process of tooth development, recent progress in mechanism studies, clinical investigations, and potential treatment approaches based on developmental biology. It provides a reference for the diagnosis and treatment of tooth number abnormality in clinical practice and facilitates the translation of basic research to clinical application.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2023)
Article
Cell & Tissue Engineering
Mingxiang Cai, Baichuan Xiao, Fujun Jin, Xiaopeng Xu, Yuwei Hua, Junhui Li, Pingping Niu, Meijing Liu, Jiaqi Wu, Rui Yue, Yong Zhang, Zuolin Wang, Yongbiao Zhang, Xiaogang Wang, Yao Sun
Summary: Deep teaming is revolutionizing peptide drug development. This study successfully utilized an unsupervised deep learning model to learn a semantic pattern of osteogenic proteins and generated a bone-forming oligopeptide with strong effects in multiple mouse models. The research established a DL-based oligopeptide discovery strategy and demonstrated its utility from cytological screening to animal experimental verification.