Review
Biochemistry & Molecular Biology
Adrijan Ivanusec, Jernej Sribar, Igor Krizaj
Summary: This study provides an overview of the diverse functions of secreted phospholipases A(2) (sPLA(2s)) as enzymes and ligands in biological processes, and presents the latest discoveries related to sPLA(2)-binding proteins (sPLA(2)-BPs). The structural diversity of sPLA(2)-BPs suggests that sPLA(2s) are promiscuous proteins, and their roles in cellular transport, signaling, and regulation of enzymatic activity are discussed. Due to their multifunctionality, sPLA(2s) show great potential as pharmacological targets for the development of diagnostic and therapeutic procedures.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Bruno Marques Vieira, Vitoria Santorio de Sao Jose, Paulo Soares Niemeyer, Vivaldo Moura-Neto
Summary: This study aimed to evaluate the interaction between eosinophils and glioblastoma cells, the mechanism of potential killing of glioblastoma cells by eosinophils, and how allergy/asthma could improve the prognosis of glioblastoma. The study found that eosinophils can be recruited and activated by glioblastoma cells via GM-CSF signaling, leading to inhibition of glioblastoma cell metabolism, proliferation, and migration through Fas/FasL pathway and induction of cell death. Cysteinyl leukotrienes present in asthmatic serum enhance glioblastoma cell migration and proliferation, but also activate eosinophils that suppress glioblastoma cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Maria Mangini, Rosa D'Angelo, Caterina Vinciguerra, Christine Payre, Gerard Lambeau, Barbara Balestrieri, Julia F. F. Charles, Stefania Mariggio
Summary: This study investigates the role of group IIA secreted phospholipase A(2) (sPLA(2)-IIA) in osteoclast bone-resorption activity and syncytium formation. The results suggest that sPLA(2)-IIA is involved in the regulation of osteoclast maturation and fusion through both catalytic-dependent and independent mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yoshitaka Taketomi, Yuki Endo, Takayoshi Higashi, Remi Murase, Tomio Ono, Choji Taya, Tetsuyuki Kobayashi, Makoto Murakami
Summary: Tissue-resident mast cells play important roles in allergic reactions and their maturation is regulated by a complex interaction between secreted phospholipase A(2) and prostaglandin D2. The absence of sPLA2-III in mast cells leads to immature mast cells and reduced allergic responses, with implications for acute anaphylactic reactions and contact hypersensitivity.
Article
Medicine, Research & Experimental
Etienne Dore, Charles Joly-Beauparlant, Satoshi Morozumi, Alban Mathieu, Tania Levesque, Isabelle Allaeys, Anne-Claire Duchez, Nathalie Cloutier, Mickael Leclercq, Antoine Bodein, Christine Payre, Cyril Martin, Agnes Petit-Paitel, Michael H. Gelb, Manu Rangachari, Makoto Murakami, Laetitia Davidovic, Nicolas Flamand, Makoto Arita, Gerard Lambeau, Arnaud Droit, Eric Boilard
Summary: sPLA(2)-IIA exerts its effects on the immune system by acting on the intestinal microbiota and its lipidome. Changes in the composition of the intestinal microbiota and bacterial-derived lipid mediators are potentially involved in this process.
Review
Immunology
Makoto Murakami, Hiroyasu Sato, Yoshitaka Taketomi
Summary: Individual sPLA(2)s in the PLA(2) superfamily play different roles in immune responses and related diseases through their unique enzymatic specificity and tissue/cellular distributions. Mass spectrometric lipidomics can help identify the substrates and products of sPLA(2)s in specific tissue microenvironments, providing valuable insights into immune responses and associated diseases.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Melanie Dacheux, Soraya Chaouch, Alonso Joy, Amandine Labat, Christine Payre, Agnes Petit-Paitel, Franck Bihl, Isabelle Lagrange, Philippe Grellier, Lhousseine Touqui, Gerard Lambeau, Christiane Deregnaucourt
Summary: Transgenic mice overexpressing hGIIA sPLA(2) show a lower peak of parasitaemia compared to wild-type littermates, potentially by releasing toxic lipids from oxidized lipoproteins and promoting a sustained innate immune response. Infection leads to increases in leukocytes, inflammatory cells, and pro-inflammatory cytokines in TG mice, along with significantly higher levels of Th1 cytokines IFN-gamma and IL-2.
Article
Biochemistry & Molecular Biology
Michael S. Kuefner, Erin Stephenson, Mladen Savikj, Heather S. Smallwood, Qingming Dong, Christine Payre, Gerard Lambeau, Edwards A. Park
Summary: The study shows that PLA2G2A enhances adipose tissue thermogenesis through increased substrate delivery and mitochondrial content in BAT, as demonstrated by the metabolic analysis and expression of UCP1 protein.
Article
Immunology
Anne Lise Ferrara, Maria Bova, Angelica Petraroli, Daniela Marasco, Christine Payre, Sara Fortuna, Francesco Palestra, Renato Ciardi, Gianni Marone, Giuseppe Spadaro, Gerard Lambeau, Stefania Loffredo
Summary: High levels of hGIIA are associated with inflammatory diseases, while C1-INH inhibits hGIIA and plays a role in immune cell function. However, hGIIA and C1-INH interact with each other and affect their respective functions.
IMMUNOLOGIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Danushki Herath, Benjamin Even, Mathilde Oranger, Roberta Foresti, Dulce Papy, Laurent Boyer, Jorge Boczkowski, Maylis Dagouassat
Summary: This study reveals that lung fibroblast senescence is involved in the pathophysiology of COPD, but the specific mechanisms are poorly understood. The researchers found that secreted phospholipases (sPLA2) are released by senescent cells and can induce cell senescence. However, the role of sPLA2 in fibroblast senescence in COPD is still unknown.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Haruka Hakoi, Yoshimi Miki, Saki Nomura, Kimiko Nakajima, Chiaki Terashima-Murase, Takuya Takeichi, Shigetoshi Sano, Masashi Akiyama, Shin-ichi Sakasegawa, Makoto Murakami, Kei Yamamoto
Summary: Epidermal lipids, specifically P-LPE, play a crucial role in regulating psoriasis, and their removal can alleviate inflammation and hyperplasia. P-LPE levels serve as a lipid biomarker for predicting and preventing psoriasis.
Article
Biochemistry & Molecular Biology
Masaya Koganesawa, Munehiro Yamaguchi, Sachin K. Samuchiwal, Barbara Balestrieri
Summary: Macrophages activated by IL-4 (M2) or LPS+IFN gamma (M1) have distinct lipid metabolism profiles, with Pla2g5 playing a key role in this process. Pla2g5 is involved in phospholipid metabolism and eicosanoid production in both types of activated macrophages.
Article
Entomology
Tian Li, Gemin Wang, Wei He, Guiqin Li, Chunyang Wang, Jiamei Zhao, Pan Chen, Meiwei Guo, Ping Chen
Summary: In this study, the role of insect immune-associated phospholipase A(2) (PLA(2)) in melanization in silkworms was investigated. PLA(2) was found to regulate melanization through the modulation of BmDDC protein expression. This study provides new insights into the complex immune networks of insects.
Article
Biochemistry & Molecular Biology
Adrijan Ivanusec, Jernej Sribar, Adrijana Leonardi, Maja Zorovic, Marko Zivin, Igor Krizaj
Summary: Alzheimer's disease is a progressive form of dementia characterized by increased expression of GIIA and dysfunction of neuronal mitochondria. This study found that rat GIIA and snake venom Atx have similar molecular effects on neuronal mitochondria, suggesting that high levels of GIIA in AD tissue may lead to its translocation into neurons and mitochondria, inhibiting respiratory chain activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mona Alonazi, Najeh Krayem, Mona G. Alharbi, Arwa Ishaq A. Khayyat, Humidah Alanazi, Habib Horchani, Abir Ben Bacha
Summary: This study identified and characterized a new type II secreted phospholipase A(2) with unique characteristics. The enzyme showed high activity and stability, and exhibited dose-dependent cytotoxic effects on various human cancer cell lines, while potentially having angiogenic activity as well.
Article
Medicine, Research & Experimental
Etienne Dore, Charles Joly-Beauparlant, Satoshi Morozumi, Alban Mathieu, Tania Levesque, Isabelle Allaeys, Anne-Claire Duchez, Nathalie Cloutier, Mickael Leclercq, Antoine Bodein, Christine Payre, Cyril Martin, Agnes Petit-Paitel, Michael H. Gelb, Manu Rangachari, Makoto Murakami, Laetitia Davidovic, Nicolas Flamand, Makoto Arita, Gerard Lambeau, Arnaud Droit, Eric Boilard
Summary: sPLA(2)-IIA exerts its effects on the immune system by acting on the intestinal microbiota and its lipidome. Changes in the composition of the intestinal microbiota and bacterial-derived lipid mediators are potentially involved in this process.
Review
Cell Biology
Fengying Tang, Jourdan E. Brune, Mary Y. Chang, Stephen R. Reeves, William A. Altemeier, Charles W. Frevert
Summary: The extracellular matrix plays a critical role in cellular processes in the lungs, with the proteoglycan versican being a key regulator in lung diseases. However, the mechanisms of how versican interacts with its binding partners and forms contextual extracellular matrices are still not fully understood.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Physiology
Chi F. Hung, Carole L. Wilson, Yu-Hua Chow, Wayne C. Liles, Sina A. Gharib, William A. Altemeier, Lynn M. Schnapp
Summary: This study investigates the role of pericyte-like cells in lung fibrosis by using a model of pericyte-like cell ablation. The results show that pericyte-like cell ablation does not affect lung fibrosis but reduces acute lung inflammation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Tong Zhang, Phi Duong, Remwilyn Dayuha, Christopher J. Collins, Erika Beckman, Jenny Thies, Irene Chang, Christina Lam, Angela Sun, Anna I. Scott, John Thompson, Aranjeet Singh, Hamid Khaledi, Michael H. Gelb, Si Houn Hahn
Summary: This study developed a novel proteomics-based assay for rapid and accurate second-tier screening of Pompe disease (PD) and mucopolysaccharidosis type I (MPS I). The assay enables the identification of severe cases who need immediate clinical follow up and treatment, with a fast turnaround time.
MOLECULAR GENETICS AND METABOLISM
(2022)
Letter
Pediatrics
Joanne Kurtzberg, Dietrich Matern, Joseph J. Orsini, Michael Gelb, Stacy Pike-Langenfeld, Lesa Brackbill, Anna Grantham, Anthony C. Steyermark
Article
Biochemistry & Molecular Biology
Conlan Kreher, Jacob Favret, Nadav Weinstock, Malabika Maulik, Xinying Hong, Michael H. Gelb, Lawrence Wrabetz, M. Laura Feltri, Daesung Shin
Summary: Krabbe disease is a result of deficiency in lysosomal galactosylceramidase enzyme, causing accumulation of certain substances. Recent studies show that the dysfunction of myelinating glia and neuronal axons play a role in Krabbe disease. In this study, neuron-specific Galc knockout model enhances our understanding of the disease and suggests the importance of neuronal GALC in maintaining neuronal function independently.
Letter
Allergy
Ryan C. Murphy, Yu-Hua Chow, Ying Lai, Taha Al-Shaikhly, Daniel H. Petroni, Michele Black, Jessica A. Hamerman, Adam Lacy-Hulbert, Adrian M. Piliponsky, Teal S. Hallstrand
Article
Biochemistry & Molecular Biology
Courtney J. Rouse, Kimberley Hawkins, Nadia Kabbej, Justin Dalugdug, Aishwarya Kunta, Mi-Jung Kim, Shinichi Someya, Zachary Herbst, Michael Gelb, Isabella Dinelli, Elizabeth Butterworth, Darin J. Falk, Erinn Rosenkrantz, Hamza Elmohd, Hamid Khaledi, Samar Mowafy, Frederick Ashby, Coy D. Heldermon
Summary: Mucopolysaccharidosis type IIIB (MPS IIIB) is a lysosomal storage disease caused by gene mutations that result in defective NAGLU protein. The study demonstrates the potential of AAV8 for gene delivery in the treatment of MPS IIIB, with improved expression and distribution compared to other AAV serotypes. The administration of AAVtcm8-coNAGLU leads to correction of lifespan, auditory deficits, increased CNS NAGLU activity, and reduced lysosomal storage levels in the mouse model of MPS IIIB.
HUMAN MOLECULAR GENETICS
(2023)
Article
Neurosciences
Diego Iacono, Shunsuke Koga, Hui Peng, Arulmani Manavalan, Jessica Daiker, Monica Castanedes-Casey, Nicholas B. Martin, Aimee R. Herdt, Michael H. Gelb, Dennis W. Dickson, Chris W. Lee
Summary: Krabbe Disease is an autosomal recessive disorder caused by mutations in the GALC gene, leading to demyelination in the central and peripheral nervous systems. Research shows that GALC protein is mainly located in oligodendrocytes in the cerebral white matter, associated with myelination. Neuropathological changes associated with KD onset and severity were observed.
NEUROBIOLOGY OF DISEASE
(2022)
Editorial Material
Critical Care Medicine
Ryan C. Murphy, Eric D. Morrell, Teal S. Hallstrand
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Kellen Hirsch, Stephanie Nolley, David D. Ralph, Ying Zheng, William A. Altemeier, Christopher J. Rhodes, Nicholas W. Morrell, Martin R. Wilkins, Peter J. Leary, Samuel G. Rayner
Summary: This study compared the differences in inflammatory and angiogenic biomarker profiles among patients with different etiologies of pulmonary arterial hypertension (PAH) and their association with clinical outcomes. The results showed that patients with connective tissue disease-associated PAH (CTD-PAH) had significantly elevated levels of inflammatory and angiogenic biomarkers. Six biomarkers were associated with mortality in the entire PAH cohort, with IL-6 and NT-proBNP remaining significant after controlling for multiple comparisons.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Review
Physiology
Samuel G. Rayner, Chi F. Hung, W. Conrad Liles, William A. Altemeier
Summary: Pericytes are microvascular mural cells that play a significant role in vascular development and homeostasis. They are also key mediators of the host response to injury, displaying cellular plasticity and involvement in inflammatory processes.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Physiology
Mary Y. Chang, Jourdan E. Brune, Michele Black, William A. Altemeier, Charles W. Frevert
Summary: Studies of pulmonary inflammation require unique considerations due to the complex structure and composition of the lungs. This study introduces a novel combination of methodologies that enables precise localization of immune cells and supports an unbiased exploratory approach to investigating diverse immune cell populations during pulmonary inflammation. The methodology utilizes cellular autofluorescence to aid in the resolution and identification of immune cell populations.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Daniele Vinciguerra, Madeline B. Gelb, Heather D. Maynard
Summary: Trehalose, a widely used disaccharide in biopharmaceutical, food, and cosmetic industries, has properties that make it suitable for stabilizing and protecting substances. Researchers have developed various methods to create trehalose-based polymers and materials that outperform trehalose itself. These have found applications as protein stabilizers, gene delivery systems, and agents for preventing amyloid aggregate formation.
Article
Genetics & Heredity
Zackary M. Herbst, Leslie Urdaneta, Terri Klein, Barbara K. Burton, Khaja Basheeruddin, Hsuan-Chieh Liao, Maria Fuller, Michael H. Gelb
Summary: This study supports the use of second-tier GAG analysis of newborn DBS, especially the endogenous disaccharide method, as part of NBS to reduce the false positive rate.
INTERNATIONAL JOURNAL OF NEONATAL SCREENING
(2022)