期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 285, 期 43, 页码 32744-32750出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.137588
关键词
-
资金
- Deutsche Forschungsgemeinschaft
- Universite des Sciences et Technologies de Lille 1
- Institut Federatif de Recherche [147]
- Centre National de la Recherche Scientifique (Unite Mixte de Recherche CNRS) [8576]
- Groupement des Entreprises Francaises dans la Lutte Contre le Cancer and Association pour la Recherche Sur le Cancer [1113]
- Deutscher Akademischer Austausch Dienst/Egide
We showed that the production of tumor necrosis factor (TNF) alpha by macrophages in response to Toxoplasma gondii glycosylphosphatidylinositols (GPIs) requires the expression of both Toll-like receptors TLR2 and TLR4, but not of their coreceptor CD14. Galectin-3 is a beta-galactoside-binding protein with immune-regulatory effects, which associates with TLR2. We demonstrate here by using the surface plasmon resonance method that the GPIs of T. gondii bind to human galectin-3 with strong affinity and in a dose-dependent manner. The use of a synthetic glycan and of the lipid moiety cleaved from the GPIs shows that both parts are involved in the interaction with galectin-3. GPIs of T. gondii also bind to galectin-1 but with a lower affinity and only through the lipid moiety. At the cellular level, the production of TNF-alpha induced by T. gondii GPIs in macrophages depends on the expression of galectin-3 but not of galectin-1. This study is the first identification of a galectin-3 ligand of T. gondii origin, and galectin-3 might be a co-receptor presenting the GPIs to the TLRs on macrophages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据