Article
Biochemistry & Molecular Biology
Jie Zhang, Yijun Yang, Xinhua Li, Gen Li, Takuya Mizukami, Yanli Liu, Yuan Wang, Guoqiang Xu, Heinrich Roder, Li Zhang, Zeng-jie Yang
Summary: PDLIM3 plays a crucial role in ciliogenesis and hedgehog signaling transduction in SHH-MB cells, promoting cell proliferation and tumor growth. It can serve as a molecular marker for defining MB.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Immunology
Wencui Wan, Yang Long, Xuemin Jin, Qiuming Li, Weiwei Wan, Hongzhuo Liu, Yu Zhu
Summary: This study demonstrated that miR-200a may serve as a potential therapeutic target in DR through down-regulating PDLIM1, significantly affecting cell viability and inflammation.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Pinxue Xie, Qisheng You, Jiang Zhu, Wuxiang Xie, Ping Wei, Siquan Zhu, Yunhui Du, Xinxiao Gao
Summary: This study aimed to investigate the role of PDLIM1 on retinal vascular endothelial cells in diabetic retinopathy (DR). The results showed that PDLIM1 was mainly expressed in specific layers of the retina and played a protective role in DR by negatively regulating Wnt3a, improving cell apoptosis, migration, and invasion. Therefore, PDLIM1 might be a promising therapeutic target for DR treatment.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Michael D. Healy, Joanna Sacharz, Kerrie E. McNally, Calum McConville, Vikas A. Tillu, Ryan J. Hall, Molly Chilton, Peter J. Cullen, Mehdi Mobli, Rajesh Ghai, David A. Stroud, Brett M. Collins
Summary: SNX17 controls the endosomal recycling of transmembrane cargo proteins through its association with the Commander trafficking complex and the PDLIM family of proteins. The interaction between SNX17 and PDLIM is mediated by a unique peptide interaction involving electrostatic contacts and a conserved proline-containing loop sequence. This interaction may play a role in regulating the activity of SNX17 in conjunction with Commander and actin-rich endosomal trafficking domains.
Article
Biochemistry & Molecular Biology
Wenxia Yan, Guanhao Chen, Jianchao Li
Summary: This study demonstrates the impact of mutations on the stability and target binding capability of Harmonin, and provides structural insights into the possible liquid-liquid phase separation process of Harmonin.
Article
Immunology
Fangwei Leng, Wenxiang Zhang, Ricardo N. Ramirez, Juliette Leon, Yi Zhong, Lifei Hou, Koichi Yuki, Joris van der Veeken, Alexander Y. Rudensky, Christophe Benoist, Sun Hur
Summary: It has been discovered that FoxP3 can exist in two different dimerization forms, head-to-head dimerization and swap dimerization, with the former being associated with functional specificity and the latter being associated with functional impairment.
Review
Biochemistry & Molecular Biology
Michael D. Healy, Brett M. Collins
Summary: PDZ and LIM domain proteins play important roles in the actin cytoskeleton and are involved in actin organization and function in metazoans. They act as scaffolds, linking various proteins to actin and its binding partner alpha-actinin. In addition to their well-known role in actin organization, emerging evidence suggests their involvement in actin-dependent membrane trafficking in the endosomal system.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Review
Oncology
Xinyuan Jiang, Zhiyong Xu, Sujing Jiang, Huan Wang, Mingshu Xiao, Yueli Shi, Kai Wang
Summary: PDZ-LIM family proteins are scaffolding proteins that contain PDZ and LIM interaction domains. The PDZ and LIM domains function as scaffolds to bind to various proteins. PDLIMs are evolutionarily conserved proteins found in different species. They mediate the interaction of signal molecules and are involved in important physiological processes such as cell differentiation, proliferation, migration, and maintenance of cellular structural integrity. Dysregulation of PDLIMs has been linked to tumor formation and development. This paper reviews and integrates the current knowledge on PDLIMs, describing the structure and function of PDZ and LIM domains and the role of PDLIMs in tumor development.
Article
Multidisciplinary Sciences
Boglarka Zambo, Bastien Morlet, Luc Negroni, Gilles Trave, Gergo Gogl
Summary: This article introduces a method called native holdup (nHU), which can directly estimate the equilibrium binding constants of protein interactions from cell extracts. Compared to other methods, nHU requires less sample preparation and can be combined with various analytical methods, making it widely applicable.
Article
Biochemistry & Molecular Biology
Baptiste Colcombet-Cazenave, Florence Cordier, Yanlei Zhu, Guillaume Bouvier, Eleni Litsardaki, Louise Laserre, Marie S. Prevost, Bertrand Raynal, Celia Caillet-Saguy, Nicolas Wolff
Summary: Hearing relies on the transduction of sound-evoked vibrations into electrical signals, and the proteins ADGRV1 and PDZD7 are involved in the formation and function of stereocilia. PDZD7 inhibits the GPCR activity of ADGRV1 during hair cell development through an unknown mechanism. The N-terminal PDZ domains of PDZD7 interact with the cytoplasmic domain of ADGRV1, and their stability and binding properties are affected by deafness-causing mutations in PDZD7.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Himanshu Singh, Chandan K. Das, Benjamin C. Buchmuller, Lars Schaefer, Daniel Summerer, Rasmus Linser
Summary: Researchers have discovered that 5-methylcytosine (mC) and its TET-oxidized derivatives exist in CpG dyads of mammalian DNA and regulate cell fate, but how they function as distinct regulatory signals is not well understood. Designing readers that selectively recognize these novel 'CpG duplex marks' presents a challenge due to their unprecedented selectivity requirements. This study demonstrates the importance of precise conformational plasticity for the selectivity of a designer reader for an oxidized CpG duplex mark.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Yudan Zeng, Dongtao Lin, Mengqian Gao, Guoxia Du, Yongming Cai
Summary: The protein PDLIM2 plays an important role in various cancers and is closely associated with immune infiltration. High expression of PDLIM2 is significantly correlated with tumor grade and immune cell infiltration.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Celia Caillet-Saguy, Nicolas Wolff
Summary: ACE2 is a main receptor for SARS-CoV-2 entry to the host cell, and its interaction with PDZ could facilitate virus entry. The study identified potential interactions between ACE2 and neuronal proteins, which may be involved in the neurological symptoms of COVID-19.
Article
Biochemistry & Molecular Biology
Celia Caillet-Saguy, Fabien Durbesson, Veronica V. Rezelj, Gergo Gogl, Quang Dinh Tran, Jean-Claude Twizere, Marco Vignuzzi, Renaud Vincentelli, Nicolas Wolff
Summary: Small linear motifs targeting the PDZ domains have been identified at the C terminus of SARS-CoV-2 proteins E, 3a, and N. By profiling the human PDZome, potential human protein targets of SARS-CoV-2 have been identified, some of which may play roles in viral replication and immune responses.
Article
Multidisciplinary Sciences
Alice H. Reis, Bo Xiang, Olga Ossipova, Keiji Itoh, Sergei Y. Sokol
Summary: The interaction between Wtip and SSX2IP proteins is essential for cell junction remodeling and morphogenetic processes during neurulation. Targeted proximity biotinylation (TPB) can be a useful method for identifying protein-protein interactions in cells.
Review
Cell Biology
Rebeca Piatniczka Iglesia, Mariana Brandao Prado, Rodrigo Nunes Alves, Maria Isabel Melo Escobar, Camila Felix de Lima Fernandes, Ailine Cibele dos Santos Fortes, Maria Clara da Silva Souza, Jacqueline Marcia Boccacino, Giovanni Cangiano, Samuel Ribeiro Soares, Joao Pedro Alves de Araujo, Deanna Marie Tiek, Anshika Goenka, Xiao Song, Jack Ryan Keady, Bo Hu, Shi Yuan Cheng, Marilene Hohmuth Lopes
Summary: Unconventional protein secretion (UPS), which is an alternative secretory mechanism associated with stress-inducing conditions, allows the secretion of proteins without a signal peptide. UPS studies have been increasingly growing in the literature and have implications in the biology of several diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anshika Goenka, Deanna Marie Tiek, Xiao Song, Rebeca Piatniczka Iglesia, Minghui Lu, Bo Hu, Shi-Yuan Cheng
Summary: Research in cancer biology has predominantly focused on protein-coding genes, but the discovery of non-coding RNAs (ncRNAs) has shed light on their crucial role in various biological processes and human diseases including cancer. Dysregulated ncRNA expression affects cancer initiation, progression, and therapy resistance. This review emphasizes the recent developments and advances in ncRNA biology relevant to glioma tumorigenesis and therapy response, highlighting the potential of ncRNAs as biomarkers and therapeutic agents in glioma.
Article
Oncology
Matthew McCord, Elizabeth Bartom, Kirsten Burdett, Aneta Baran, Frank D. Eckerdt, Irina Balyasnikova, Kathleen McCortney, Thomas Sears, Shi-Yuan Cheng, Jann N. Sarkaria, Roger Stupp, Amy B. Heimberger, Atique Ahmed, Charles David James, Craig Horbinski
Summary: This study developed therapy-resistant glioblastoma patient-derived xenografts (PDX) by exposing them to radiation and temozolomide. These PDX models reflect key features of recurrent glioblastoma and can serve as valuable tools for testing new therapies.
Article
Oncology
Ayechew A. Getu, Ming Zhou, Shi-Yuan Cheng, Ming Tan
Summary: Cancer, a complex and dynamic disease, remains a leading cause of death in humans. The Mammalian Sterile 20-Like Kinase 4 (MST4 or STK26) plays a crucial role in cell migration and polarity, and is involved in various processes such as tumor cell proliferation, migration, invasion, survival, and cancer metastasis. MST4 interacts with PDCD10 to promote tumor growth and migration, and phosphorylates ATG4B to mediate autophagy signaling and contribute to treatment resistance. Overall, MST4 functions as an oncogene and shows potential as a therapeutic target.
Article
Oncology
Anshika Goenka, Xiao Song, Deanna Tiek, Rebeca Piatniczka Iglesia, Minghui Lu, Chang Zeng, Craig Horbinski, Wei Zhang, Bo Hu, Shi-Yuan Cheng
Summary: In this study, a novel oncogenic long noncoding RNA (lncRNA) called LINC02283 was identified. It is highly expressed in glioma patients with PDGFRA mutation and is associated with poor prognosis. LINC02283 interacts with PDGFRA to enhance its signaling pathway and promote oncogenesis in glioblastoma. These findings provide strong evidence for the potential of lncRNAs as therapeutic targets.
Article
Hematology
Shirong Li, Jing Fu, Christopher J. Walker, Jun Yang, Divaya Bhutani, Rajshekhar Chakraborty, Niha Mamillapalli, Markus Y. Mapara, Yosef Landesman, Suzanne Lentzsch
Summary: Selinexor is an inhibitor of XPO1 and reduces the levels of eIF4E, IKZF1, and c-MYC proteins in multiple myeloma cells. Combination treatment of Selinexor with pharmacologic inhibitors of protein translation shows synergistic effects in vitro.
Article
Multidisciplinary Sciences
Jing Fu, Shirong Li, Huihui Ma, Jun Yang, Gabriel M. Pagnotti, Lewis M. Brown, Stephen J. Weiss, Markus Y. Mapara, Suzanne Lentzsch
Summary: PD-1H functions as a potential MMP-13 receptor on osteoclasts, mediating MMP-13 induced cytoskeleton reorganization, fusogenesis and bone resorption, and further regulating osteolytic lesions.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Michael A. Sun, Rui Yang, Heng Liu, Wenzhe Wang, Xiao Song, Bo Hu, Nathan Reynolds, Kristen Roso, Lee H. Chen, Paula K. Greer, Stephen T. Keir, Roger E. McLendon, Shi-Yuan Cheng, Darell D. Bigner, David M. Ashley, Christopher J. Pirozzi, Yiping He
Summary: In this study, researchers discovered that clemastine, a non-oncology drug for allergy relief, can inhibit the proliferation and promote differentiation of brain tumor-initiating cells (BTICs). They also identified the essential role of Emopamil-binding protein (EBP) in maintaining the BTIC population. These findings provide valuable insights for developing new treatments for glioblastoma.
Meeting Abstract
Oncology
Michael Sun, Rui Yang, Heng Liu, Wenzhe Wang, Xiao Song, Bo Hu, Nathan Reynolds, Kristen Roso, Lee Chen, Paula Greer, Shi-Yuan Cheng, David Ashley, Christopher Pirozzi, Yiping He
Meeting Abstract
Oncology
Deanna Tiek, Shi-Yuan Cheng, Xiao Song, Anshika Goenka, Rebeca Iglesia, Bo Hu, Rebecca Riggins
Meeting Abstract
Oncology
Anshika Goenka, Xiao Song, Deanna Tiek, Bo Hu, Shi-Yuan Cheng
Meeting Abstract
Clinical Neurology
Deanna Tiek, Xiao Song, Anshika Goenka, Rebeca Iglesia, Bo Hu, Rebecca Riggins, Shi-Yuan Cheng
ANNALS OF NEUROLOGY
(2022)
Meeting Abstract
Clinical Neurology
Xiao Song, Tianzhi Huang, Deanna Tiek, Shunichiro Miki, Anshika Goenka, Rebeca Iglesia, Frank B. Furnari, Bo Hu, Shi-Yuan Cheng
ANNALS OF NEUROLOGY
(2022)
Meeting Abstract
Clinical Neurology
Anshika Goenka, Xiao Song, Deanna M. Tiek, Bo Hu, Shi-Yuan Cheng
ANNALS OF NEUROLOGY
(2022)
Review
Oncology
Deanna Tiek, Shi-Yuan Cheng
Summary: This review discusses different strategies to combat cancer drug resistance, including synthetic lethality and modulation of the tumor microenvironment. By understanding the relationship between targetable mutations and metabolism in tumor cells and their surrounding microenvironment, smarter drugs can be designed to fight cancer drug resistance.
CANCER DRUG RESISTANCE
(2022)