Article
Biochemistry & Molecular Biology
Qingling Yuan, Yuxia Fan, Zheng Liu, Xiaoming Wang, Meng Jia, Yongqiang Dong, Zushi Geng, Jian Zheng, Xiubo Lu
Summary: This study aimed to investigate the effects of PLEKHG4 on the progression of thyroid cancer (TC). The results showed that PLEKHG4 was up-regulated in human TC tissues and its level was correlated with clinicopathological parameters of TC patients. In vitro and in vivo experiments revealed that PLEKHG4 promoted TC cell proliferation, migration, invasion, and epithelial-mesenchymal transition via activating the RhoGTPases pathway. The ubiquitin-conjugating enzyme E2O (UBE2O) facilitated the ubiquitination and degradation of PLEKHG4. Thus, targeting the UBE2O/PLEKHG4/RhoGTPases axis could be a potential therapeutic strategy for TC.
Article
Biochemistry & Molecular Biology
Alexander E. Aleshin, Yong Yao, Amer Iftikhar, Andrey A. Bobkov, Jinghua Yu, Gregory Cadwell, Michael G. Klein, Chuqiao Dong, Laurie A. Bankston, Robert C. Liddington, Wonpil Im, Garth Powis, Francesca M. Marassi
Summary: This study elucidates the structural basis for PLEKHA7 recognition of membrane-bound PIPs, showing that the interaction is multivalent and induces PIP clustering. The findings reveal the central role of membrane assembly in mediating protein-PIP association and provide insights into the function of the PH domain in PLEKHA7 signaling, adhesion, and nanoclustering.
Article
Multidisciplinary Sciences
Veronika Thallmair, Lea Schultz, Wencai Zhao, Siewert J. Marrink, Dominik Oliver, Sebastian Thallmair
Summary: Phosphoinositides (PIs), especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P-2], play a crucial role in targeting proteins to cilia. This study discovered that the tubby domain, which is essential for this targeting process, binds to PI(4,5)P-2 through two binding sites.
Article
Biochemistry & Molecular Biology
Daniel L. De la Cruz, Laszlo Prokai, Katalin Prokai-Tatrai
Summary: Through the use of a homology model and optimization of binding sites, this study revealed an allosteric binding site for a TRH antagonist on the TRH receptor, rather than the active site. This allosteric site exhibited stronger binding affinity and has the potential to play a significant role in agonist and antagonist actions.
Article
Neurosciences
Xiang-Ying Xu, Fei-Fei Zhang, Jun Gan, Mao-Yin Zhang, Zhong-Shan Shen, Qing Guo, Yue Teng, Jun-Wei Ji, Jun -Li Cao, Qiong-Yao Tang, Zhe Zhang
Summary: The protein TMEM206 is the molecular basis of the extracellular proton-activated Cl2 channel (PAC), which plays a crucial role in neuronal death during ischemia-reperfusion. The mechanism of proton sensitivity in the PAC channel is unclear, but two acidic amino acid residues were found to form a proton-sensitive site in the channel. Chloral hydrate was also found to activate the channel, and knockout mice resistant to chloral hydrate-induced sedation and hypnosis were observed. This study provides insights into the activation mechanism of the hPAC channel and identifies a potential drug target in chloral hydrate.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Rui Liu, Yunlu Kang, Lei Chen
Summary: Soluble guanylate cyclase (sGC) is the primary receptor for nitric oxide (NO) and its binding to the distal site of haem in the fully activated state has been observed through high-resolution cryo-EM maps. The binding of NO induces a conformational change in sGC and activates its cyclase activity.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Andreas H. Larsen, Laura H. John, Mark S. P. Sansom, Robin A. Corey
Summary: This article reviews recent attempts to characterize the interactions between peripheral membrane proteins (PMPs) and biological membranes. The article compares different methods used to identify and analyze lipid-binding sites from simulation data and highlights recent work on characterizing the energetics of these interactions using free energy calculations. The authors describe how improvements in methodologies and computing power will continue to contribute to the field of molecular dynamics simulations.
BIOSCIENCE REPORTS
(2022)
Article
Biochemical Research Methods
Fatemeh Mazloumi Gavgani, Malene Skuseth Slinning, Andrea Papdine Morovicz, Victoria Smith Arnesen, Diana C. Turcu, Sandra Ninzima, Clive S. D'Santos, Aurelia E. Lewis
Summary: Polyphosphoinositides (PPIns) are essential lipid signaling molecules with functions in both cytoplasm and nucleus. This study identified 179 potential interacting partners of PtdIns(3,4,5)P-3 in the isolated nuclei, revealing their roles in RNA processing/splicing, cytokinesis, protein folding, and DNA repair. Notably, some of the interacting proteins have dual functions in rRNA processes and DNA repair, providing insights into the molecular mechanisms of PtdIns(3,4,5)P-3-mediated interactions in the nucleus and nucleolus.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Ryan J. Boyd, Tien L. Olson, James D. Zook, Derek Stein, Manuel Aceves, Wan-Hsin Lin, Felicia M. Craciunescu, Debra T. Hansen, Panos Z. Anastasiadis, Abhishek Singharoy, Petra Fromme
Summary: This study investigated the protein expression and purification of human Syx and revealed its folded state and binding ability to RhoA. Additionally, computational simulations were used to explore the interactions between GEF activity and the PH domain, providing insights into the allosteric modulation of Dbl homology GEF proteins.
Article
Multidisciplinary Sciences
Tao Wang, Xiaowei Zhan, Chun-Hui Bu, Stephen Lyon, David Pratt, Sara Hildebrand, Jin Huk Choi, Zhao Zhang, Ming Zeng, Kuan-wen Wang, Emre Turer, Zhe Chen, Duanwu Zhang, Tao Yue, Ying Wang, Hexin Shi, Jianhui Wang, Lei Sun, Jeff SoRelle, William McAlpine, Noelle Hutchins, Xiaoming Zhan, Maggy Fina, Rochelle Gobert, Jiexia Quan, McKensie Kreutzer, Stephanie Arnett, Kimberly Hawkins, Ashley Leach, Christopher Tate, Chad Daniel, Carlos Reyna, Lauren Prince, Sheila Davis, Joel Purrington, Rick Bearden, Jennifer Weatherly, Danielle White, Jamie Russell, Qihua Sun, Miao Tang, Xiaohong Li, Lindsay Scott, Eva Marie Y. Moresco, Gerald M. McInerney, Gunilla B. Karlsson Hedestam, Yang Xie, Bruce Beutler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Multidisciplinary Sciences
Heath G. Pascoe, Stephen Gutowski, Hua Chen, Chad A. Brautigam, Zhe Chen, Paul C. Sternweis, Xuewu Zhang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biology
Oleg A. Volkov, Lisa Kinch, Carson Ariagno, Xiaoyi Deng, Shihua Zhong, Nick Grishin, Diana R. Tomchick, Zhe Chen, Margaret A. Phillips
Article
Medicine, Research & Experimental
Risheng Ye, Ruth Gordillo, Mengle Shao, Toshiharu Onodera, Zhe Chen, Shiuhwei Chen, Xiaoli Lin, Jeffrey A. SoRelle, Xiaohong Li, Miao Tang, Mark P. Keller, Regina Kuliawat, Alan D. Attie, Rana K. Gupta, William L. Holland, Bruce Beutler, Joachim Herz, Philipp E. Scherer
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Article
Chemistry, Medicinal
Oleg A. Volkov, Anthony J. Brockway, Stephen A. Wring, Michael Peel, Zhe Chen, Margaret A. Phillips, Jef K. De Brabander
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Olugbenga Dada, Stephen Gutowski, Chad A. Brautigam, Zhe Chen, Paul C. Sternweis
JOURNAL OF STRUCTURAL BIOLOGY
(2018)
Article
Cell Biology
Shreoshi Majumdar, Tae Kim, Zhe Chen, Sarah Munyoki, Shih-Chia Tso, Chad A. Brautigam, Luke M. Rice
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Biochemistry & Molecular Biology
Kodai Hara, Ge Zheng, Qianhui Qui, Hong Liu, Zhuqing Ouyang, Zhe Chen, Diana R. Tomchick, Hongtao Yu
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Zhe Chen, William Holland, John M. Shelton, Aktar Ali, Xiaoming Zhan, Sungyong Won, Wataru Tomisato, Chen Liu, Xiaohong Li, Eva Marie Y. Moresco, Bruce Beutler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Multidisciplinary Sciences
John C. Hunter, Deepak Gurbani, Scott B. Ficarro, Martin A. Carrasco, Sang Min Lim, Hwan Geun Choi, Ting Xie, Jarrod A. Marto, Zhe Chen, Nathanael S. Gray, Kenneth D. Westover
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biochemistry & Molecular Biology
Marcin Maziarz, Anthony Leyme, Arthur Marivin, Alex Luebbers, Prachi P. Patel, Zhe Chen, Stephen R. Sprang, Mikel Garcia-Marcos
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Cell Biology
Faxiang Li, Joanna E. Raczynska, Zhe Chen, Hongtao Yu
Article
Biochemistry & Molecular Biology
Wei Peng, Amanda K. Casey, Jessie Fernandez, Emily M. Carpinone, Kelly A. Servage, Zhe Chen, Yang Li, Diana R. Tomchick, Vincent J. Starai, Kim Orth
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Lianying Jiao, Murtada Shubbar, Xin Yang, Qi Zhang, Siming Chen, Qiong Wu, Zhe Chen, Josep Rizo, Xin Liu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Zhe Chen, Steven Gutowski, Paul C. Sternweis
