Article
Chemistry, Medicinal
Dianyi Li, Zheng Wang, Yong Yu, Huirong Li, Wei Luo, Bo Chen, Guoqing Niu, Haitao Ding
Summary: A novel GH2 beta-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli and showed stable soluble form. The purified enzyme had a specific activity of 96.827 U mg(-1) at 30 degrees C using ONPG as a substrate. It had an optimum pH of 7.0 and an optimum temperature of 50 degrees C. The enzyme's activity was enhanced by some divalent cations and inhibited by EDTA. Despite being derived from a cold-adapted strain, the enzyme exhibited considerable stability against various physical and chemical elements. It also showed activity towards rare substrates. The enzyme is a promising candidate for the industrial application of beta-galactosidase.
Article
Biochemistry & Molecular Biology
Jing Guo, Yi Wang, Wenjun Gao, Xinrou Wang, Xin Gao, Zaiwei Man, Zhiqiang Cai, Qing Qing
Summary: The novel glycoside hydrolase family 46 chitosanase SaCsn46A from Streptomyces avermitilis has been successfully cloned and functionally expressed. This enzyme shows high stability and activity in moderate temperature and pH conditions, with the ability to hydrolyze a variety of polysaccharides linked by beta-1,4-glycosidic bonds. Moreover, it can be enhanced by divalent metal ions like Mg2+ and Mn2+, making it a promising candidate for producing glucosamine and chitooligosaccharides from chitosan.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Nicholas G. S. McGregor, Joan Coines, Valentina Borlandelli, Satoko Amaki, Marta Artola, Alba Nin-Hill, Daniel Linzel, Chihaya Yamada, Takatoshi Arakawa, Akihiro Ishiwata, Yukishige Ito, Gijsbert A. van der Marel, Jeroen D. C. Codee, Shinya Fushinobu, Herman S. Overkleeft, Carme Rovira, Gideon J. Davies
Summary: The discovery of zinc-dependent retaining glycoside hydrolases presents unresolved mechanistic questions, particularly the controversy surrounding the proposed mechanism involving a zinc-coordinated cysteine nucleophile and a thioglycosyl enzyme intermediate. A synthesized beta-l-arabinofuranosidase inhibitor reacts exclusively with the cysteine thiol catalytic nucleophiles of GH families 127 and 146, supporting further research into the breakdown of the thioglycosyl enzyme intermediate. X-ray crystal structures and simulations provide insight into the mechanisms of zinc-coordinated cysteine as the catalytic nucleophile and the energetics of this unique metalloenzyme clan.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Paeton L. Wantuch, Satya Jella, Jeremy A. Duke, Jarrod J. Mousa, Bernard Henrissat, John Glushka, Fikri Y. Avci
Summary: Paenibacillus sp. 32352 produces an enzyme, Pn3Pase, capable of degrading the capsular polysaccharide of Streptococcus pneumoniae serotype 3. Recent studies have shown the potential of Pn3Pase as a protection against highly virulent S. pneumoniae serotype 3 infections. Biochemical analysis and mutagenesis studies have revealed that Pn3Pase establishes a new CAZy GH family and identified catalytic residues and essential domains for function. This study contributes to understanding the mechanistic and structural aspects of Pn3Pase, establishing it as a potential therapeutic approach against type 3 S. pneumoniae infections.
Article
Chemistry, Medicinal
Yuya Kumagai, Hideki Kishimura, Weeranuch Lang, Takayoshi Tagami, Masayuki Okuyama, Atsuo Kimura
Summary: In this study, the glycoside hydrolase family 17 beta-1,3-glucanase of Vibrio vulnificus (VvGH17) was characterized by preparing mutant enzymes. The C-terminus was found to be important for the enzyme's activity and substrate-binding ability, while the N-terminus contributed to the multimeric form of VvGH17. These findings provide insights into the structure and function of GH17 beta-1,3-glucanases.
Article
Biochemistry & Molecular Biology
Tianle Qu, Chunyue Zhang, Zhen Qin, Liqiang Fan, Lihua Jiang, Liming Zhao
Summary: Aminooligosaccharides have various biological activities and can be widely used in food, pharmaceutical, and cosmetic industries. The cloned enzyme AoNagase showed high catalytic efficiency and good stability, making it a promising candidate for the production of aminooligosaccharides.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yanxin Wang, Ding Li, Chaonan Dong, Yuqiang Zhao, Lei Zhang, Fan Yang, Xianfeng Ye, Yan Huang, Zhoukun Li, Zhongli Cui
Summary: In this study, a novel glycoside hydrolase family 55 beta-1,3-glucanase (AcGluA) from Archangium sp. strain AC19 was cloned and expressed in Escherichia coli, showing high activity on laminarin within a stable pH range and temperature. AcGluA hydrolyzed laminarin into a series of oligosaccharides and demonstrated significant biocontrol efficacy on rice seedlings against Magnaporthe oryzae. This study provides a new perspective for the development of green biopesticides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Guanchen Liu, Jingjing Shen, Yaoguang Chang, Xuanwei Mei, Guangning Chen, Yuying Zhang, Changhu Xue
Summary: A new bacterial enzyme, Fun174A, was discovered to degrade sulfated fucans in sea cucumbers, exhibiting significant biological and biomedical activities.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Physical
Jorick Franceus, Jolien Lormans, Lore Cools, Matthias D'hooghe, Tom Desmet
Summary: The study demonstrates significant phosphorylase activity can be established in GH3 glycoside hydrolases through single-site substitutions and further optimized through mutational paths. These findings suggest new insights into developing phosphorylases and provide a novel approach for future research in this field.
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a β-1,3-glucanase named BsGlc157A was identified from Bacteroides sp. M27. It exhibited optimal catalytic activity at pH 6.0 and 40 degrees C. BsGlc157A adopted a classic (beta/alpha)(8) TIM-barrel structure and two catalytic residues were confirmed. Moreover, BsGlc157A showed inhibitory effects on the hyphal growth of fruit pathogenic fungi, highlighting its potential as a biocontrol agent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiao Zhao, Jeremy Esque, Isabelle Andre, Michael J. O'Donohue, Regis Faure
Summary: The GH-51 alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus exhibits versatile catalytic properties, with mutants showing increased yields and regioselectivity in the synthesis of furanobiosides. The study demonstrates the potential usefulness of these mutants for glycosynthesis, particularly in the production of biologically-relevant glycoconjugates. Through in-depth characterization, it was found that mutants can maximize the yield of homo-disaccharides by using higher substrate concentrations.
BIOORGANIC CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Ju-Hee Cha, Minsun Hong, Chang-Jun Cha
Summary: In this study, a fungal beta-glycosidase belonging to subfamily 4 of GH family 30 (GH30) was characterized with hydrolysis and transglycosylation activities towards various substrates, representing the first fungal beta-glycosidase with transglycosylation activities in subfamily 4 of GH30.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Kedar Sharma, Carlos M. G. A. Fontes, Shabir Najmudin, Arun Goyal
Summary: The alpha-L-arabinofuranosidase from Clostridium thermocellum displays a modular architecture, enhancing catalytic efficiency. Molecular dynamics simulation analysis reveals higher flexibility in its C-terminal module. Small angle X-ray scattering analysis indicates an elongated structure of the enzyme.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Casey Crooks, Nathan J. Bechle, Franz J. St John
Summary: This study characterized a non-reducing terminal (NRT)-specific xylobiohydrolase AcXbh30A, which strictly cleaves xylobiose from the NRT with minimal other hydrolysis products under optimized reaction conditions. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Nataliia Machushynets, Somayah S. Elsayed, Chao Du, Maxime A. Siegler, Mercedes de la Cruz, Olga Genilloud, Thomas Hankemeier, Gilles P. van Wezel
Summary: Streptomycetes are important producers of bioactive natural products, and this study describes the discovery of novel actinomycins L-1 and L-2 produced by Streptomyces sp. MBT27. The study also provides insights into the structure and biosynthetic pathway of actinomycin L.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Chiho Suzuki-Minakuchi, Kohei Kawazuma, Jun Matsuzawa, Delyana Vasileva, Zui Fujimoto, Tohru Terada, Kazunori Okada, Hideaki Nojiri
Editorial Material
Biochemistry & Molecular Biology
Wade Abbott, Orly Alber, Ed Bayer, Jean-Guy Berrin, Alisdair Boraston, Harry Brumer, Ryszard Brzezinski, Anthony Clarke, Beatrice Cobucci-Ponzano, Darrell Cockburn, Pedro Coutinho, Mirjam Czjzek, Bareket Dassa, Gideon John Davies, Vincent Eijsink, Jens Eklof, Alfons Felice, Elizabeth Ficko-Blean, Geoff Pincher, Thierry Fontaine, Zui Fujimoto, Kiyotaka Fujita, Shinya Fushinobu, Harry Gilbert, Tracey Gloster, Ethan Goddard-Borger, Ian Greig, Jan-Hendrik Hehemann, Glyn Hemsworth, Bernard Henrissat, Masafumi Hidaka, Ramon Hurtado-Guerrero, Kiyohiko Igarashi, Takuya Ishida, Stefan Janecek, Seino Jongkees, Nathalie Juge, Satoshi Kaneko, Takane Katayama, Motomitsu Kitaoka, Naotake Konno, Daniel Kracher, Anna Kulminskaya, Alicia Lammerts van Bueren, Sine Larsen, Junho Lee, Markus Linder, Leila LoLeggio, Roland Ludwig, Ana Luis, Mirko Maksimainen, Brian Mark, Richard McLean, Gurvan Michel, Gurvan Michel, Cedric Montanier, Marco Moracci, Haruhide Mori, Hiroyuki Nakai, Wim Nerinckx, Takayuki Ohnuma, Richard Pickersgill, Kathleen Piens, Tirso Pons, Etienne Rebuffet, Peter Reilly, Magali Remaud-Simeon, Brian Rempel, Kyle Robinson, David Rose, Juha Rouvinen, Wataru Saburi, Yuichi Sakamoto, Mats Sandgren, Fathima Shaikh, Yuval Shoham, Franz St John, Jerry Stahlberg, Michael Suits, Gerlind Sulzenbacher, Tomomi Sumida, Ryuichiro Suzuki, Birte Svensson, Toki Taira, Ed Taylor, Takashi Tonozuka, Breeanna Urbanowicz, Gustav Vaaje-Kolstad, Wim Van den Ende, Annabelle Varrot, Maxime Versluys, Florence Vincent, David Vocadlo, Warren Wakarchuk, Tom Wennekes, Rohan Williams, Spencer Williams, David Wilson, Stephen Withers, Katsuro Yaoi, Vivian Yip, Ran Zhang
Article
Biochemistry & Molecular Biology
Tomohiko Matsuzawa, Satoshi Kaneko, Naomi Kishine, Zui Fujimoto, Katsuro Yaoi
JOURNAL OF BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Tomohiko Matsuzawa, Masahiro Watanabe, Yusuke Nakamichi, Zui Fujimoto, Katsuro Yaoi
JOURNAL OF STRUCTURAL BIOLOGY
(2019)
Article
Immunology
Satoko Watanabe, Zui Fujimoto, Masaji Mase
Article
Biochemistry & Molecular Biology
Koji Teramoto, Sosyu Tsutsui, Tomoko Sato, Zui Fujimoto, Satoshi Kaneko
Summary: The substrate specificities of glycoside hydrolase families from Bacillus halodurans C-125 were investigated, showing that different xylosidases exhibit varying efficiency and order in hydrolyzing xylooligosaccharides.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Kasumi Takeuchi, Wataru Tsuchiya, Zui Fujimoto, Kosumi Yamada, Nobutaka Someya, Toshimasa Yamazaki
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Zui Fujimoto, Naomi Kishine, Koji Teramoto, Sosyu Tsutsui, Satoshi Kaneko
Summary: This study elucidated the substrate specificity and subsite structures of a GH11 xylanase from Streptomyces olivaceoviridis. SoXyn11B showed no affinity for the subsites at the ends of the glycosidic bond, with the sugar-binding energy highest at subsite -2. The xylanase produced different branched oligosaccharides compared to a GH10 xylanase from the same species.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Yuji Ashikawa, Zui Fujimoto, Kengo Inoue, Hisakazu Yamane, Hideaki Nojiri
Summary: The study resolved two crystal structures of class III CARDO-R, revealing the absence of FAD in the type II structure and significant conformational changes to the FAD-binding domain and C-terminus. Docking simulation of NADH into CARDO-R-J3 suggested that shifts of residues at the C-terminus may facilitate electron transfer. Differences in domain arrangement compared to RO reductases from the ferredoxin-NADP reductase family may correspond to differences in structures of redox partners.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zui Fujimoto, Le Thi Thu Hong, Naomi Kishine, Nobuhiro Suzuki, Keitarou Kimura
Summary: The study revealed that the dominant allele of yabJ gene caused by a single amino acid mutation leads to changes in B. subtilis production, and the structural change may be related to the inversion of the fifth beta-strand, affecting its ligand-binding capability.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ryuichiro Suzuki, Zui Fujimoto, Satoshi Kaneko, Tsunemi Hasegawa, Atsushi Kuno
JOURNAL OF APPLIED GLYCOSCIENCE
(2018)
Article
Biotechnology & Applied Microbiology
Tomoko Maehara, Haruka Yagi, Tomoko Sato, Mayumi Ohnishi-Kameyama, Zui Fujimoto, Kei Kamino, Yoshiaki Kitamura, Franz St John, Katsuro Yaoi, Satoshi Kaneko
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Zui Fujimoto, Nobuhiro Suzuki, Naomi Kishine, Hitomi Ichinose, Mitsuru Momma, Atsuo Kimura, Kazumi Funane
BIOCHEMICAL JOURNAL
(2017)
Article
Biochemistry & Molecular Biology
Zui Fujimoto, Naomi Kishine, Nobuhiro Suzuki, Ryuichiro Suzuki, Daiki Mizushima, Mitsuru Momma, Keitarou Kimura, Kazumi Funane
BIOSCIENCE REPORTS
(2017)
Article
Biotechnology & Applied Microbiology
Hitomi Ichinose, Ryuichiro Suzuki, Takatsugu Miyazaki, Keitarou Kimura, Mitsuru Momma, Nobuhiro Suzuki, Zui Fujimoto, Atsuo Kimura, Kazumi Funane
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)