Article
Biochemistry & Molecular Biology
Vladimir I. Timofeev, Ilya V. Fateev, Maria A. Kostromina, Yuliya A. Abramchik, Irina D. Konstantinova, Vladimir V. Volkov, Dmitry D. Lykoshin, Olga O. Mikheeva, Tatiana I. Muravieva, Roman S. Esipov, Inna P. Kuranova
Summary: Two recombinant purine nucleoside phosphorylases from Thermus thermophilus HB27 were purified and characterized. TthPNPI is specific to guanosine while TthPNPII is specific to adenosine. The oligomeric structures of these enzymes, as well as the key residues in their active sites, were analyzed. The crystal structure of TthPNPII was determined and its active site cavity was examined. The hexameric structure of TthPNPII, which is different from other purine nucleoside phosphorylases, was observed for the first time.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Physical
Jinhui Tan, Zhongzui Wang, Guangzhao Li, Huicong Hu, Jie Li, Rui Han, Dongyan Zhang
Summary: This study investigates the electrochemically driven phase transition of LiCoO2 and experimental verification shows that doping can eliminate the phase transition to improve its actual capacity to the theoretical value.
Article
Chemistry, Multidisciplinary
Yifan Dai, Christian F. Chamberlayne, Marco S. Messina, Christopher J. Chang, Richard N. Zare, Lingchong You, Ashutosh Chilkoti
Summary: Biomolecular condensates play a crucial role in various cellular processes. This study reveals that the interface of condensates establishes an electric potential gradient, leading to spontaneous redox reactions in vitro and in living cells. These findings highlight the importance of the condensate interface in modulating biochemical activities.
Article
Chemistry, Physical
Mingzhao Zhuo, Gregory Offer, Monica Marinescu
Summary: Nickel-rich layered oxides are commonly used as positive electrode materials in lithium-ion batteries, but their degradation mechanisms are not well understood. In this study, a particle-level model is proposed to describe the structural degradation due to phase transition, including loss of active material, loss of lithium inventory, and resistance increase. The model is incorporated into a cell-level P2D model to investigate the effects of degradation on capacity fade. It is found that the loss of positive electrode active materials dominates the capacity loss, while trapped cyclable lithium in the degraded shell does not directly contribute to the loss.
JOURNAL OF POWER SOURCES
(2023)
Article
Biochemistry & Molecular Biology
Maribel Lara-Chica, Alejandro Correa-Saez, Rafael Jimenez-Izquierdo, Martin Garrido-Rodriguez, Francisco J. Ponce, Rita Moreno, Kimberley Morrison, Chiara Di Vona, Krisztina Arato, Carla Jimenez-Jimenez, Rosario Morrugares, M. Lienhard Schmitz, Susana de la Luna, Laureano de la Vega, Marco A. Calzado
Summary: DYRK2 is a serine/threonine kinase that phosphorylates CDC25A to control its degradation, while CDC25A can influence the phosphorylation status of DYRK2, forming a feedback regulatory loop that plays a key role in cell cycle regulation and genomic integrity.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Chemistry, Multidisciplinary
Leonhard Karger, Kevin Synnatschke, Simon Settele, Yvonne J. Hofstetter, Tim Nowack, Jana Zaumseil, Yana Vaynzof, Claudia Backes
Summary: The degradation kinetics of WS2 nanosheet ensembles in the liquid phase were investigated, revealing that thermal degradation is slower and requires higher activation energy than photoinduced degradation. It was observed that using SC as a surfactant can sufficiently suppress photoinduced degradation, while chemical passivation achieved through the addition of cysteine can prevent degradation by coordinating to defects on the nanosheets and stabilizing oxides on the surface.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Inorganic & Nuclear
Fredric G. Svensson, Bogdan Cojocaru, Zhen Qiu, Vasile Parvulescu, Tomas Edvinsson, Gulaim A. Seisenbaeva, Carmen Tiseanu, Vadim G. Kessler
Summary: A molecular precursor approach was developed for titania nanopowders modified with rare-earth elements. The addition of Eu3+ and Sm3+ stabilized the anatase phase and improved photocatalytic activity under visible light illumination, attributing to doping of the band structure of TiO2 and to the surface chemistry of the REE-modified titania.
INORGANIC CHEMISTRY
(2021)
Article
Automation & Control Systems
Zhang Ao, Wang Zhihua, Wu Qiong, Liu Chengrui
Summary: This study aims to evaluate the reliability of phase-transition degrading systems. The researchers construct a generalized stochastic degradation model and consider the phase-varying stochastic property and phase-varying nonlinearity. Simulation studies and a practical case study are conducted to validate the model and analyze the impact of model misspecification.
JOURNAL OF SYSTEMS ENGINEERING AND ELECTRONICS
(2022)
Article
Materials Science, Ceramics
Shafique Ahmed, Man Zhang, Vladimir Koval, Lifong Zou, Zhijian Shen, Riqing Chen, Bin Yang, Haixue Yan
Summary: ZrO2-based ceramics are commonly used in biomedical applications for their color, biocompatibility, and excellent mechanical properties. The study on LTD in 3Y-TZP ceramic powders revealed a tetragonal-to-monoclinic phase transition driven by degradation. Terahertz nondestructive probe shows promise as a method to investigate LTD in zirconia ceramics.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2022)
Article
Electrochemistry
Hirona Hasegawa, Yuji Ishado, Shigeto Okada, Minoru Mizuhata, Hideshi Maki, Masaki Matsui
Summary: The study found that Ca substitution in NaxCoO2 affects its capacity fading during charge and discharge cycles, mainly due to the migration of Co3+/4+ ions. Although Ca substitution does not completely prevent phase transitions, it does improve capacity retention.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Materials Science, Multidisciplinary
Qin Zhou, Yue Huang, Yan Zhang, Chong Xu, Weiya Huang, Kai Yang, Xirong Chen, Yongcai Zhang
Summary: AgIn5S8 (AIS) nanoparticles with excellent photocatalytic performance were synthesized using a low temperature co-precipitation process. The sample obtained after 2 hours of reaction time showed the best photocatalytic degradation performance towards various organic pollutants. The presence of defects in the sample contributed to the enhancement of photocatalytic performance, as indicated by various characterization methods.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Polymer Science
Kaiting Wu, Xiaobin Chen, Siyi Gu, Shuquan Cui, Xiaowei Yang, Lin Yu, Jiandong Ding
Summary: A series of amphiphilic triblock copolymers composed of poly(ethylene glycol) and poly(epsilon-caprolactone-co-5-alkyl delta-lactone)s with distinct alkyl side groups were synthesized. These copolymers exhibited different aqueous behaviors in response to temperature variations, such as forming a reversed thermosensitive hydrogel or a normal hydrogel, depending on the length of hydrophobic side chains.
Article
Chemistry, Physical
Xin Yu, Xianqing Huang, Ying Huang, Qingge Feng, Wenfu Chen, Chuanfu Ju, Yude Du, Tengfei Bai, Dongbo Wang
Summary: The crystal phase has a significant impact on the photocatalytic activity of Bi2O3. By preparing a novel alpha/beta-Bi2O3 hetero-phase junction through a simple solvothermal calcination method, the photocatalyst exhibited the highest activity at 400 degrees Celsius, showing significant improvement in tetracycline hydrochloride degradation compared to single crystal phase Bi2O3.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Physical
Guoshuai Du, Feng Ke, Wuxiao Han, Bin Chen, Qinglin Xia, Jun Kang, Yabin Chen
Summary: This study provides a detailed analysis on the structural metastability of black arsenic and identifies its existence conditions based on thermodynamic variables. The researchers also developed a thermal annealing strategy to control the thickness of black arsenic flakes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Materials Science, Biomaterials
Chen Yuan, Shue Jin, Jiawei Wei, Jinhui Huang, Chang Liu, Xiaoyu Lei, Yi Zuo, Jidong Li, Yubao Li
Summary: The study found that temperature and liquid environment were the main factors affecting the shrinkage of electrospun PLGA membranes by influencing their glass transition. After heat stretching treatment, the glass transition temperature of the PLGA membrane increased, improving its stability significantly.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Takao Itoi, Masahiro Sugimoto, Junko Umeda, Atsushi Sofuni, Takayoshi Tsuchiya, Shujiro Tsuji, Reina Tanaka, Ryosuke Tonozuka, Mitsuyoshi Honjo, Fuminori Moriyasu, Kazuhiko Kasuya, Yuichi Nagakawa, Yuta Abe, Kimihiro Takano, Shigeyuki Kawachi, Motohide Shimazu, Tomoyoshi Soga, Masaru Tomita, Makoto Sunamura
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Multidisciplinary Sciences
Ghulam Jeelani, Dan Sato, Tomoyoshi Soga, Tomoyoshi Nozaki
SCIENTIFIC REPORTS
(2017)
Article
Food Science & Technology
Yasutoshi Akiyama, Koichi Kikuchi, Takafumi Toyohara, Eikan Mishima, Chitose Suzuki, Takehiro Suzuki, Masaaki Nakayama, Yoshihisa Tomioka, Tomoyoshi Soga, Takaaki Abe
Summary: Uremic toxins are suggested to play a role in the pathophysiology of hemodialysis (HD) patients. A comprehensive analysis using capillary electrophoresis mass spectrometry (CE-MS) identified specific uremic solutes that accumulate or are lost in HD patients compared to non-HD chronic kidney disease (CKD) patients. These findings suggest a unique profile of serum uremic solutes in HD patients that may contribute to their pathophysiology.
Article
Biochemistry & Molecular Biology
Kana Miyamoto, Akiyoshi Hirayama, Yuiko Sato, Satsuki Ikeda, Midori Maruyama, Tomoyoshi Soga, Masaru Tomita, Masaya Nakamura, Morio Matsumoto, Noriko Yoshimura, Takeshi Miyamoto
Summary: The increasing number of patients with osteoporosis and sarcopenia is a global concern, and the high medical costs suggest prevention is better than treatment. Metabolomic analysis found differences in specific metabolites in the blood of newly developed osteoporosis and sarcopenia patients, which could potentially be used to predict disease progression within four years.
Article
Oncology
Haruhiko Shindo, Narumi Harada-Shoji, Akiko Ebata, Miku Sato, Tomoyoshi Soga, Minoru Miyashita, Hiroshi Tada, Masaaki Kawai, Shinkichi Kosaka, Koji Onuki, Shin Usami, Shozo Furumoto, Shinichi Hayashi, Takaaki Abe, Takashi Suzuki, Takanori Ishida, Hironobu Sasano
Summary: The study showed that high LAT1 status in breast cancer patients is associated with tumor progression, particularly in those receiving neoadjuvant hormone therapy, and LAT1 expression is upregulated in estrogen deprivation-resistant breast carcinoma cell lines. A selective LAT1 inhibitor, JPH203, demonstrated inhibitory effects on cell proliferation in resistant cell lines, suggesting LAT1 could be a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Atsushi Kimura, Akiyoshi Hirayama, Tatsuaki Matsumoto, Yuiko Sato, Tami Kobayashi, Satsuki Ikeda, Midori Maruyama, Mari Kaneko, Mayo Shigeta, Eri Ito, Tomoya Soma, Kana Miyamoto, Tomoyoshi Soga, Masaru Tomita, Akihito Oya, Morio Matsumoto, Masaya Nakamura, Arihiko Kanaji, Takeshi Miyamoto
Summary: Ossification of the posterior longitudinal ligament (OPLL), a disease characterized by ectopic ossification of a spinal ligament, can lead to neurological disorders. This study investigated the role of hydroxyacid oxidase 1 (Hao1) in OPLL development and found that Hao1 expression decreased during osteoblast differentiation. Further experiments using Hao1-deficient mice showed that Hao1 loss did not promote ectopic ossification, but rather regulated the tricarboxylic acid (TCA) cycle metabolism.
Article
Biochemistry & Molecular Biology
Rintaro Saito, Akiyoshi Hirayama, Arisa Akiba, Yushi Kamei, Yuyu Kato, Satsuki Ikeda, Brian Kwan, Minya Pu, Loki Natarajan, Hibiki Shinjo, Shin'ichi Akiyama, Masaru Tomita, Tomoyoshi Soga, Shoichi Maruyama
Summary: Using CE-TOFMS, urinary metabolomic profiles of patients after surgery were analyzed to detect patterns specific to AKI, potentially leading to better biomarkers for early detection in the future.
Article
Biochemistry & Molecular Biology
Yukako Suzuki, Ryosuke Hayasaka, Masako Hasebe, Satsuki Ikeda, Tomoyoshi Soga, Masaru Tomita, Akiyoshi Hirayama, Hiroki Kuroda
Summary: This study identified specific small molecules expressed in the dorsal or ventral marginal zones in vertebrate gastrula, investigating their impact on cell fate. By analyzing hydrophilic metabolites and lipids, the research found differences in metabolism-related molecules between the two regions, contributing to a better understanding of cellular physiology during early embryogenesis.
Article
Biochemistry & Molecular Biology
Akiyoshi Hirayama, Takamasa Ishikawa, Haruka Takahashi, Sanae Yamanaka, Satsuki Ikeda, Aya Hirata, Sei Harada, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: High-throughput metabolomics has facilitated large-scale cohort studies. Long-term studies require multiple batch-based measurements, which necessitates sophisticated quality control to eliminate bias. In this study, liquid chromatography-mass spectrometry was used to analyze 10,833 samples in 279 batch measurements. The quantified profile included 147 lipids. Normalization using QC samples reduced the coefficient of variation for the lipids. This study provides unbiased and quantified data for large-scale metabolomics.
Article
Biochemical Research Methods
Ryuhei Kudo, Kaori Igarashi, Tomoyoshi Soga, Takamasa Ishikawa, Yasuhiro Saito
Summary: Capillary electrophoresis mass spectrometry (CE-MS) and liquid chromatography mass spectrometry (LC-MS) are two commonly used methods for metabolite analysis, but require independent sample preparation. This article presents a sample preparation protocol for breast cancer cell lines.
Article
Cell Biology
Yusuke Izuta, Toshihiro Imada, Ryuji Hisamura, Erina Oonishi, Shigeru Nakamura, Emi Inagaki, Masataka Ito, Tomoyoshi Soga, Kazuo Tsubota
Correction
Biochemistry & Molecular Biology
Elena Yukie Yoshitoshi-Uebayashi, Taro Toyoda, Katsutaro Yasuda, Maki Kotaka, Keiko Nomoto, Keisuke Okita, Kentaro Yasuchika, Shinya Okamoto, Noriyuki Takubo, Toshiya Nishikubo, Tomoyoshi Soga, Shinji Uemoto, Kenji Osafune
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Tatsuya Nishihara, Joe Inoue, Sho Tabata, Shinnosuke Murakami, Takamasa Ishikawa, Natsumi Saito, Shinji Fukuda, Masaru Tomita, Tomoyoshi Soga
Article
Biochemistry & Molecular Biology
Elena Yukie Yoshitoshi-Uebayashi, Taro Toyoda, Katsutaro Yasuda, Maki Kotaka, Keiko Nomoto, Keisuke Okita, Kentaro Yasuchika, Shinya Okamoto, Noriyuki Takubo, Toshiya Nishikubo, Tomoyoshi Soga, Shinji Uemoto, Kenji Osafune
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Endocrinology & Metabolism
Takeshi Miyamoto, Akiyoshi Hirayama, Yuiko Sato, Tami Koboyashi, Eri Katsuyama, Hiroya Kanagawa, Hiroya Miyamoto, Tomoaki Mori, Shigeyuki Yoshida, Atsuhiro Fujie, Mayu Morita, Ryuichi Watanabe, Toshimi Tando, Kana Miyamoto, Takashi Tsuji, Atsushi Funayama, Masaya Nakamura, Morio Matsumoto, Tomoyoshi Soga, Masaru Tomita, Yoshiaki Toyama