Article
Cell Biology
Wanchun Yang, Dejiang Pang, Mina Chen, Chongyangzi Du, Lanlan Jia, Luoling Wang, Yunling He, Wanxiang Jiang, Liping Luo, Zongyan Yu, Mengqian Mao, Qiuyun Yuan, Ping Tang, Xiaoqiang Xia, Yiyuan Cui, Bo Jing, Alexander Platero, Yanhui Liu, Yuquan Wei, Paul F. Worley, Bo Xiao
Summary: Rheb regulates mitochondrial energy production through PDH activation, maintaining neuronal activity balance. Cell-specific Rheb genetic models highlight the importance of the Rheb-PDH axis in modulating PDH phosphorylation/activity, acetyl-CoA, and ATP levels.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Takefumi Kimura, Sai P. Pydi, Lei Wang, Dhanush Haspula, Yinghong Cui, Huiyan Lu, Gabriele M. Koenig, Evi Kostenis, Gregory R. Steinberg, Oksana Gavrilova, Juergen Wess
Summary: This study found that by activating adipocyte G(q) signaling, the increased lipolysis in obese individuals can be prevented, leading to improved glucose and lipid homeostasis. This has important implications for the development of novel anti-diabetic drugs based on GPCR.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Nan Li, Shan Shan, Xiu-Qin Li, Ting-Ting Chen, Meng Qi, Sheng-Nan Zhang, Zi-Ying Wang, Ling-Ling Zhang, Wei Wei, Wu-Yi Sun
Summary: G protein-coupled receptor kinase 2 (GRK2) plays important roles in regulating signaling pathways associated with fibrotic diseases. Recent research suggests that GRK2 could be a potential therapeutic target for fibrotic diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Richard Sando, Thomas C. Sudhof
Summary: Latrophilin-2 and Latrophilin-3 play essential roles in synapse formation in the hippocampus by requiring GPCR signaling to activate the classical GPCR-signaling pathway.
Article
Pharmacology & Pharmacy
Shotaro Michinaga, Ayaka Nagata, Ryosuke Ogami, Yasuhiro Ogawa, Shigeru Hishinuma
Summary: This study aimed to explore how H1 receptor-mediated ERK phosphorylation is regulated by Gq proteins and arrestins. The results showed that the Gq protein pathway mainly regulates early phosphorylation reaction, while the arrestin pathway mainly regulates late phosphorylation reaction.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biology
Leonie Schnell, Alina Zubrod, Nicola Catone, Johanna Bialas, Annette Aichem
Summary: The ubiquitin-like modifier FAT10 plays an important role in protein degradation and is up-regulated in various cell types. While USE1 was previously thought to be the sole E2 enzyme for FAT10ylation, we identified additional E2 conjugating enzymes that are involved in FAT10 conjugation. This finding expands the field of FAT10 research and provides new possibilities for pharmacological interventions to regulate FAT10 conjugation under inflammatory conditions and/or viral infections.
LIFE SCIENCE ALLIANCE
(2023)
Article
Multidisciplinary Sciences
Ahmed Wagdi, Daniela Malan, Udhayabhaskar Sathyanarayanan, Janosch S. Beauchamp, Markus Vogt, David Zipf, Thomas Beiert, Berivan Mansuroglu, Vanessa Dusend, Mark Meininghaus, Linn Schneider, Bernd Kalthof, J. Simon Wiegert, Gabriele M. Konig, Evi Kostenis, Robert Patejdl, Philipp Sasse, Tobias Bruegmann
Summary: This study presents a new optogenetic tool, human Neuropsin (hOPN5), which allows for specific and repetitive manipulation of G(q) signaling with high spatio-temporal resolution. The researchers characterized the properties and G protein specificity of hOPN5 using UV light-induced IP3 generation, Ca2+ transients, and inhibition of G(IRK) channel activity. They demonstrated that light could increase the beating rate of adult hearts and induce contractions in the small intestine, both of which were dependent on G(q) signaling. Additionally, the authors showed that all-optical high-throughput screening using hOPN5 was more specific and sensitive compared to conventional pharmacological screening.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Emily E. Blythe, Mark von Zastrow
Summary: A study found that the vasoactive intestinal peptide receptor 1 (VIPR1) does not require beta-arrestin for internalization or generation of endosomal signal. Instead, beta-arrestin desensitizes the plasma membrane phase without affecting the endosomal phase, resulting in sequential cAMP peaks. This finding challenges the current understanding that endosomal signaling is inherently beta-arrestin-dependent.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Khadija Banu, Qisheng Lin, John M. Basgen, Marina Planoutene, Chengguo Wei, Anand C. Reghuvaran, Xuefei Tian, Hongmei Shi, Felipe Garzon, Aitor Garzia, Nicholas Chun, Arun Cumpelik, Andrew D. Santeusanio, Weijia Zhang, Bhaskar Das, Fadi Salem, Li Li, Shuta Ishibe, Lloyd G. Cantley, Lewis Kaufman, Kevin V. Lemley, Zhaohui Ni, John Cijiang He, Barbara Murphy, Madhav C. Menon
Summary: Shroom3 knockdown in mice induced albuminuria and foot process effacement without FSGS or podocytopenia, suggesting a protective role for AMPK activation. Our findings demonstrate the important role of Fyn in podocyte survival and glomerular volume regulation in the context of injury.
Article
Biochemistry & Molecular Biology
Matej Gazdarica, Judith Noda, Oleh Durydivka, Vendula Novosadova, Ken Mackie, Jean-Philippe Pin, Laurent Prezeau, Jaroslav Blahos
Summary: CB1R signaling is regulated by receptor desensitization and the protein SGIP1, which plays an important role in controlling CB1R function and its involvement in pathological conditions.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Patrick J. Kearney, Nicholas C. Bolden, Elizabeth Kahuno, Tucker L. Conklin, Gilles E. Martin, Gert Lubec, Haley E. Melikian
Summary: In this study, the authors used chemogenetics and conditional gene silencing to investigate the regulation of the dopamine transporter (DAT). They found that activating presynaptic Gq-coupled receptors led to rapid changes in DAT trafficking, with different effects in ventral and dorsal striatal regions. They also identified the specific mechanisms involved in DAT insertion and retrieval. In addition, they showed that DAT trafficking affects dopamine clearance and motor learning. These findings provide important insights into the regulation of DAT and its role in dopamine signaling and behaviors.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Ting-Ting Chu, Xintao Tu, Kun Yang, Jianjun Wu, Joyce J. Repa, Nan Yan
Summary: This study identifies a cGAS- and cGAMP-independent mode of STING activation that affects neuropathology and provides a therapeutic target for the treatment of Niemann-Pick disease type C.
Article
Multidisciplinary Sciences
Maximilian W. Schenk, Sam Humphrey, A. S. Md Mukarram Hossain, Mitchell Revill, Sarah Pearsall, Alice Lallo, Stewart Brown, Samuel Bratt, Melanie Galvin, Tine Descamps, Cong Zhou, Simon P. Pearce, Lynsey Priest, Michelle Greenhalgh, Anshuman Chaturvedi, Alastair Kerr, Fiona Blackhall, Caroline Dive, Kristopher K. Frese
Summary: In small cell lung cancer, acquired resistance to standard platinum-etoposide chemotherapy often occurs, with Notch and nitric oxide pathways playing a role in mediating this resistance. Upregulation of soluble guanylate cyclase in residual tumor cells is associated with chemoresistance, highlighting this pathway as a potential target for treating relapsed SCLC.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Hanako Hagio, Wataru Koyama, Shiori Hosaka, Aysenur Deniz Song, Janchiv Narantsatsral, Koji Matsuda, Tomohiro Sugihara, Takashi Shimizu, Mitsumasa Koyanagi, Akihisa Terakita, Masahiko Hibi, Suresh Jesuthasan
Summary: We examined the functionality of animal G-protein-coupled bistable rhodopsins in zebrafish by controlling them into active and inactive states using light application. The Gq-coupled spider Rh1 expressed in hindbrain reticulospinal V2a neurons increased intracellular Ca2+ levels and induced swimming behavior upon light stimulation. The Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin expressed in cardiomyocytes caused cardiac arrest, which could be suppressed by pertussis toxin or barium treatment, indicating the regulation of cardiac function by Gi/o-dependent inward-rectifier K+ channels. These rhodopsins are valuable tools for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes.
Article
Biochemistry & Molecular Biology
Tao Zhang, Yongfang Xia, Li Hu, Dongmei Chen, Chen-Ling Gan, Long Wang, Yingxue Mei, Guihua Lan, Xindong Shui, Yuan Tian, Ruomeng Li, Mi Zhang, Tae Ho Lee
Summary: The aggregation of A13 peptides into oligomers and fibrils is a key feature of Alzheimer's disease. This study reveals that A13 oligomers can upregulate DAPK1 levels and cause neuronal apoptosis and tau phosphorylation. The inhibition of DAPK1 can protect neurons against A13-induced damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Monica Dalva, Ida K. Lavik, Khadija El Jellas, Anny Gravdal, Aurelia Lugea, Stephen J. Pandol, Pal R. Njolstad, Richard T. Waldron, Karianne Fjeld, Bente B. Johansson, Anders Molven
Article
Cell Biology
Gaston Amable, Eduardo Martinez-Leon, Maria Elisa Picco, Sergio Nemirovsky, Enrique Rozengurt, Osvaldo Rey
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Cell Biology
Cintia E. Citterio, Sofia Siffo, Christian M. Moya, Mauricio Gomes Pio, Maricel F. Molina, Karen G. Scheps, Osvaldo A. Rey, Peter Arvan, Carina M. Rivolta, Hector M. Targovnik
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2020)
Article
Oncology
Mingtian Che, Soo-Mi Kweon, Jia-Ling Teo, Yate-Ching Yuan, Laleh G. Melstrom, Richard T. Waldron, Aurelia Lugea, Raul A. Urrutia, Stephen J. Pandol, Keane K. Y. Lai
Review
Gastroenterology & Hepatology
Zobeida Cruz-Monserrate, Kristyn Gumpper, Valentina Pita, Phil A. Hart, Christopher Forsmark, David C. Whitcomb, Dhiraj Yadav, Richard T. Waldron, Stephen Pandol, Hanno Steen, Vincent Anani, Natasha Kanwar, Santhi Swaroop Vege, Savi Appana, Liang Li, Jose Serrano, Jo Ann S. Rinaudo, Mark Topazian, Darwin L. Conwell
Summary: Chronic pancreatitis lacks diagnostic or prognostic biomarkers, but potential candidates have been identified. Five biomarkers showed high effectiveness in distinguishing CP cases, while 30 had moderate effectiveness. Variability in study design, criteria, and sample handling was noted, suggesting the need for standardized approaches in future research.
Article
Agriculture, Dairy & Animal Science
Brenda Florencia Gimeno, Maria Victoria Bariani, Lucia Laiz-Quiroga, Eduardo Martinez-Leon, Micaela Von-Meyeren, Osvaldo Rey, Adrian Angel Mutto, Claudia Elena Osycka-Salut
Summary: This study improved the yield of fertilization-capable sperm from equine frozen sperm by optimizing manipulation conditions and establishing a primary culture model of oviductal cells. The in vitro model demonstrated the potential fertilizing capacity of sperm released from oviductal cells, suggesting advancements in handling and selection of cryopreserved sperm for improved efficiencies in assisted reproductive techniques.
Letter
Gastroenterology & Hepatology
Marc T. Goodman, Simon K. Lo, Dhiraj Yadav, Bechien U. Wu, Laith H. Jamil, Karl K. Kwok, Georgios I. Papachristou, Elham Afghani, Yunhee Choi-Kuaea, Richard T. Waldron, Christina Lombardi, Christie Y. Jeon, Irene B. Helenowski, Ellen Richmond, Kelly Benante, Aida Habtezion, Tia Schering, Seema A. Khan, Luz M. Rodriguez, Stephen J. Pandol
Article
Biochemistry & Molecular Biology
Cheng Hu, Liyuan Yin, Zhiyao Chen, Richard T. Waldron, Aurelia Lugea, Yiyun Lin, Xiaoqian Zhai, Li Wen, Yuan-Ping Han, Stephen J. Pandol, Lihui Deng, Qing Xia
Summary: Chronic pancreatitis (CP) is closely related to irreversible fibro-inflammatory changes induced by pancreatic stellate cells (PSC), which have unique transcriptional profiles. Some genes in PSC may serve as biomarkers for CP.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Meeting Abstract
Gastroenterology & Hepatology
C. Hu, L. Yin, Z. Chen, R. T. Waldron, A. Lugea, Y. Lin, X. Zhai, Y. Han, S. J. Pandol, L. Deng, Q. Xia
Meeting Abstract
Gastroenterology & Hepatology
C. Hu, R. T. Waldron, C. Quiros, Q. Xia, B. Ji, S. J. Pandol, A. Lugea
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, S. Parker, J. Van Eyk, G. I. Papachristou, S. J. Pandol
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, L. Huo, P. Yue, J. Turkson, S. J. Pandol
Meeting Abstract
Gastroenterology & Hepatology
J. Wang, J. Wan, O. Wang, M. Jaggarapu, A. Haddock, R. T. Waldron, A. Lugea, S. J. Pandol, Y. Wang, Y. Bi, B. Ji
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, K. Stello, G. I. Papachristou, S. J. Pandol
Review
Physiology
Qiong Wei, Liang Qi, Hao Lin, Dechen Liu, Xiangyun Zhu, Yu Dai, Richard T. Waldron, Aurelia Lugea, Mark O. Goodarzi, Stephen J. Pandol, Ling Li
FRONTIERS IN PHYSIOLOGY
(2020)