Article
Chemistry, Multidisciplinary
Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Hoang Vesely, Patrick Reardon, Nikolai N. Sluchanko, Joseph S. Beckman, P. Andrew Karplus, Ryan A. Mehl, Richard B. Cooley
Summary: 14-3-3 proteins dimerize and bind phosphorylated clients to regulate their function. By introducing stable mimics of phosphorylated amino acids, researchers can study 14-3-3 function effectively. In this study, the authors improved a previous genetic code expansion system to incorporate nonhydrolyzable phosphoserine into proteins. They successfully produced biologically relevant proteins with the modified amino acid and investigated the effects of phosphorylation on protein interactions and function.
ACS CENTRAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Joshua Kim, Krista Chun, Jenna McGowan, Youjie Zhang, Piotr J. Czernik, Blair Mell, Bina Joe, Saurabh Chattopadhyay, Joseph Holoshitz, Ritu Chakravarti
Summary: Inflammatory arthritis (IA) is a common disease affecting millions worldwide. 14-3-3 zeta protein plays a role in immune suppression and extracellular remodeling, leading to previously unrecognized IA-suppressive function. Immunization with recombinant 14-3-3 zeta protein can suppress IL-1 beta levels and inhibit the progression of IA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Cuixian Li, Shen Huang, Jin Peng, Tianguo Hong, Chun Zhou, Jie Tang
Summary: In this study, a new cellular protein, 14-3-3 zeta, was found to interact with the beta subunit of GABA(A)Rs and BIG1, and co-localize with them in hippocampal neurons. Overexpression of 14-3-3 zeta increased the surface expression of BIG1 and its binding with GABA(A)R. Depletion of 14-3-3 zeta or BIG1 significantly decreased GABA(A)R expression at the cell surface and inhibited GABA-gated influx of chloride ions.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Fisheries
Rui Xue, Dinglong Yang, Yijing Han, Qinyou Deng, Xin Wang, Xiangquan Liu, Jianmin Zhao
Summary: The 14-3-3 zeta and 14-3-3 epsilon proteins play important roles in innate immunity against V. harveyi infection in mollusks, with their differential expression leading to different immune responses.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jacinth Rajendra, Atanu Ghorai, Shilpee Dutt
Summary: The study identified significant up-regulation of 14-3-3 zeta in residual resistant GBM cells, and knocking down this protein sensitized GBM cells to radiation therapy. Additionally, reduction of 14-3-3 zeta resulted in increased mitochondrial content in residual cells.
Article
Biochemistry & Molecular Biology
Sunayana Dagar, Kumari Pushpa, Diksha Pathak, Sarbani Samaddar, Anjana Saxena, Sourav Banerjee, Sivaram V. S. Mylavarapu
Summary: This study demonstrates a novel mRNA-guided mechanism of TNT formation through the maintenance of cellular 14-3-3 zeta mRNA levels by the RBP nucleolin.
Article
Oncology
Mingda Zhao, Yibing Zhang, Longfei Li, Xiaobin Liu, Wenping Zhou, Chunhui Wang, Yufu Tang
Summary: Our study found that KHDRBS3 is highly expressed in human HCC tissues and predicts poor prognosis. Knockdown of KHDRBS3 inhibits tumor growth, reduces glycolysis, enhances sensitivity to doxorubicin, and induces apoptosis in HCC cells. On the contrary, overexpression of KHDRBS3 promotes malignant behavior. KHDRBS3 interacts with YWHAZ, and the promotion of proliferation and glycolysis and the inhibition of apoptosis caused by KHDRBS3 overexpression can be reversed by silencing 14-3-3ζ.
CANCER CELL INTERNATIONAL
(2023)
Article
Cell Biology
Yuan Huang, Shi Li, Qinfeng Liu, Zhijie Wang, Shunyao Li, Lei Liu, Weiwei Zhao, Kai Wang, Rui Zhang, Longfei Wang, Ming Wang, Declan William Ali, Marek Michalak, Xing-Zhen Chen, Cefan Zhou, Jingfeng Tang
Summary: This study reveals that lymphocyte-specific protein tyrosine kinase (LCK) directly interacts with transient receptor potential melastatin 8 (TRPM8) and enhances its phosphorylation at Y1022. LCK positively regulates TRPM8 channel function by increasing TRPM8 multimerization. Additionally, the interaction between TRPM8 and 14-3-3 zeta positively modulates channel multimerization. The phosphorylation of TRPM8 at Y1022 plays a crucial role in the proliferation, migration, and tumorigenesis of pancreatic cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Biology
Lei Song, Jingjing Luo, Hongou Wang, Dan Huang, Yunhao Tan, Yao Liu, Yingwu Wang, Kaiwen Yu, Yong Zhang, Xiaoyun Liu, Dan Li, Zhao-Qing Luo
Summary: In this study, a new bacterial effector Lem8 was found to modulate the function of host cytoskeleton and mediate the intracellular replication of Legionella pneumophila.
Article
Hematology
Rainer Kaiser, Afra Anjum, Lisa Kammerer, Quentin Loew, Anastassia Akhalkatsi, Dario Rossaro, Raphael Escaig, Augustin Droste Zu Senden, Ben Raude, Michael Lorenz, Christoph Gold, Kami Pekayvaz, Thomas Brocker, Jan Kranich, Julian Walter Holch, Karsten Spiekermann, Steffen Massberg, Florian Gaertner, Leo Nicolai
Summary: Platelets are important in both thrombosis and hemostasis, as well as inflammation. They are involved in migration and host defense, and this migration is regulated by anisotropic myosin IIa-activity and polarized actin polymerization. The signaling pathway involving G alpha 13, c-Src/14-3-3 zeta, and lamellipodium formation plays a key role in platelet migration, and inhibitors of this pathway, such as dasatinib, interfere with platelet migration without affecting other platelet functions. This has implications in inflammation-associated hemorrhage and platelet dysfunction in patients treated with dasatinib.
Article
Neurosciences
Irene Navarro-Lobato, Mariam Masmudi-Martin, Maria E. Quiros-Ortega, Celia Gaona-Romero, Marta Carretero-Rey, Cristina Rey Blanes, Zafar U. Khan
Summary: By overexpressing a memory enhancer called RGS14(414) in the perirhinal cortex of male rats, object recognition memory (ORM) was enhanced and short-term ORM was converted into long-term ORM within a key temporal window of 40 to 60 minutes post-exposure. During this conversion, 14-3-3 zeta upregulation facilitated the process, while beyond 60 minutes, it mediated the consolidation of stable memory into long-lasting ORM by regulating BDNF signaling.
JOURNAL OF NEUROSCIENCE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Liqing Lu, Jing Zhang, Wei Fan, Yuan Li, Jiaohong Wang, Tony W. H. Li, Lucia Barbier Torres, Jose M. Mato, Ting Liu, Ekihiro Seki, Michitaka Matsuda, Maria Lauda Tomasi, Neil A. Bhowmick, Heping Yang, Shelly C. Lu
Summary: The study reveals the critical role of the interaction between MAT alpha 1 and 14-3-3ζ in liver tumorigenesis, showing their reciprocal negative regulation influencing tumor progression.
Article
Cell Biology
Peipei Wu, Bin Zhang, Xinye Han, Yaoxiang Sun, Zixuan Sun, Linli Li, Xinru Zhou, Qian Jin, Peiwen Fu, Wenrong Xu, Hui Qian
Summary: Research has found that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-ex) have antioxidant and anti-inflammatory effects against UV radiation-induced skin photodamage by activating SIRT1 to reduce oxidative stress and promote autophagy, thereby alleviating cytotoxic damage. The delivery of 14-3-3 zeta protein by hucMSC-ex plays a cytoprotective role through modulation of a SIRT1-dependent antioxidant pathway. Overall, hucMSC-ex may serve as a potential agent for preventing or treating UV radiation-induced skin photodamage and aging.
Article
Cell Biology
Mija Marinkovic, Matilda Sprung, Ivana Novak
Summary: The study showed that BNIP3L dimerization is crucial for recruiting autophagosomes efficiently for selective removal of mitochondria. The interplay between BNIP3L phosphorylation and dimerization is necessary for initiation and progression of BNIP3L-dependent mitophagy.
Article
Andrology
Alaa Eisa, Souvik Dey, Alex Ignatious, Wesam Nofal, Rex A. Hess, Manabu Kurokawa, Douglas Kline, Srinivasan Vijayaraghavan
Summary: Spermatogenesis is a complex biological process involving the synthesis and activation of proteins, with 14-3-3 proteins playing critical roles in regulating cell cycle and apoptosis. Among the seven isoforms, 14-3-3 epsilon has been identified as essential for normal sperm function and male fertility, with specific roles in spermatogenesis and sperm motility.