Article
Biochemistry & Molecular Biology
Rong Xuan, Jianmin Wang, Qing Li, Yanyan Wang, Shanfeng Du, Qingling Duan, Yanfei Guo, Peipei He, Zhibin Ji, Tianle Chao
Summary: This study conducted transcriptome sequencing of goat-mammary-gland tissue at different developmental stages to reveal the expression characteristics and molecular functions of circRNAs during mammary involution. A total of 11,756 circRNAs were identified, with 2528 circRNAs expressed in all three stages. Gene ontology analysis showed functional diversity of circRNAs' source genes. Furthermore, differentially expressed circRNAs and circRNA-miRNA-mRNA ceRNA regulatory networks were identified, providing insights into the regulatory role of circRNAs in mammary cell involution and remodeling.
Article
Agriculture, Dairy & Animal Science
Rong Xuan, Tianle Chao, Xiaodong Zhao, Aili Wang, Yunpeng Chu, Qing Li, Yilin Zhao, Zhibin Ji, Jianmin Wang
Summary: This study investigated the structural and transcriptome changes of mammary gland tissue in nonlactating dairy goats during late lactation, the dry period, and late gestation. The results showed significant structural changes and cell apoptosis in the mammary gland tissue. Transcriptome analysis identified 1,381 genes with differential expression at the three developmental stages. Genes related to cell growth, apoptosis, immunity, nutrient transport, synthesis, and metabolism exhibited adaptive transcriptional changes.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rong Xuan, Xiaodong Zhao, Qing Li, Yilin Zhao, Yanyan Wang, Shanfeng Du, Qingling Duan, Yanfei Guo, Zhibin Ji, Tianle Chao, Jianmin Wang
Summary: This study investigated the expression characteristics and molecular functions of long noncoding RNA (lncRNA) in goat mammary gland tissue. The results showed that lncRNA expression is differentially regulated at different stages of mammary gland involution and remodeling, and lncRNAs play a role in regulating various physiological activities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Amritha Varshini Hanasoge Somasundara, Matthew A. Moss, Mary J. Feigman, Chen Chen, Samantha L. Cyrill, Michael F. Ciccone, Marygrace C. Trousdell, Macy Vollbrecht, Siran Li, Jude Kendall, Semir Beyaz, John E. Wilkinson, Camila O. dos Santos
Summary: Pregnancy induces expansion of NKT-like immune cells and upregulation of immune signaling molecules in mammary epithelial cells. Lack of CD1d expression or activated NKT cells may lead to mammary oncogenesis.
Article
Developmental Biology
Wen-Cheng Chung, Sean E. Egan, Keli Xu
Summary: Notch3 plays a role in promoting mammary luminal cell specification, but recent studies show it also has a tumor-suppressive function. In mouse mammary glands, Notch3 is expressed in the luminal compartment during development, but switches to basal cells after weaning. Deletion of Notch3 leads to abnormal mammary gland development and formation of tumors.
Article
Genetics & Heredity
Yue Zhang, Qiong Wu, Jidan Liu, Xiaopeng An, Binyun Cao
Summary: This study found that Chi-miR-8516 indirectly or directly regulates casein secretion and lipid formation in goat mammary epithelial cells by targeting STC1 and promoting the phosphorylation of MAPK. The research illustrates an axis that regulates the synthesis of milk components.
Article
Developmental Biology
Johanna Englund, Alexandra Ritchie, Leander Blaas, Hanne Cojoc, Nalle Pentinmikko, Julia Dohla, Sharif Iqbal, Manuel Patarroyo, Pekka Katajisto
Summary: This study demonstrates the crucial role of Lama5 in mammary gland development and remodeling, potentially through affecting the Wnt4-mediated signaling pathway between HR+ luminal cells and basal epithelial cells, which in turn impacts gland growth and differentiation.
Article
Biotechnology & Applied Microbiology
Nannan Jiang, Chaoqun Wu, Yongtao Li, Jianxin Liu, Yuan Yuan, Hengbo Shi
Summary: In this study, two distinct miRNA expression patterns were found to be associated with mammary regenerative involution. Differentially expressed miRNAs were primarily involved in tissue self-renewal and enriched in pathways related to vesicle-mediated transport, tissue development, tube development, vasculature development, and epithelial development.
Article
Developmental Biology
Jessica Hitchcock, Katherine Hughes, Sara Pensa, Bethan Lloyd-Lewis, Christine J. Watson
Summary: Post-lactational mammary gland regression involves programmed cell death, ECM breakdown, and adipocyte redifferentiation. Tumor growth is impacted by the stage of involution at which cells are implanted in a syngeneic model. Older first-time mothers have increased breast cancer risk, with initial tumor growth faster in aged mice compared to young mice.
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Biochemistry & Molecular Biology
Lei Tian, Shancheng Guo, Zhiye Zhao, Yuxu Chen, Chunmei Wang, Qingzhang Li, Ye Li
Summary: The study reveals that miR-30a-3p plays a crucial role in regulating autophagy in the mammary glands of mice during lactation and involution. It negatively regulates autophagy by targeting Atg12 gene in mammary epithelial cells. Further experiments confirmed that overexpression of miR-30a-3p inhibits autophagy and alters mammary structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Taylor R. Rutherford, Alan M. Elder, Traci R. Lyons
Summary: The study reveals that SEMA7A promotes anoikis resistance in mammary epithelial cells and plays a role during mammary gland involution. SEMA7A is primarily expressed in cells expressing alpha 6-integrin, and a decrease in luminal progenitor cells is observed in SEMA7A-/- mice during involution.
CELL DEATH & DISEASE
(2021)
Article
Agriculture, Dairy & Animal Science
Silvia Pares, Olivia Cano-Garrido, Alex Bach, Neus Ferrer-Miralles, Antonio Villaverde, Elena Garcia-Fruitos, Anna Aris
Summary: The study aimed to test the role of injecting metalloproteinase-9 during the cow dry period, but found that it did not enhance immune response or mammary gland involution as expected.
Review
Cell Biology
Shalini Jaswal, Manoj Kumar Jena, Vijay Anand, Avinash Jaswal, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty
Summary: The mammary gland undergoes repeated cyclic changes throughout the life of mammals. Cattle and buffalo are major milk-producing livestock species that play essential roles in farming communities. The development of the ruminant mammary gland is highly dynamic and involves multiple stages. However, the molecular processes underlying cow udder development have not been thoroughly investigated. This review focuses on the morphological and molecular changes during various stages of cow udder development, providing insights into the physiological differences between cattle and other mammalian species.
Article
Multidisciplinary Sciences
Lisa Suwandhi, Irem Altun, Ruth Karlina, Viktorian Miok, Tobias Wiedemann, David Fischer, Thomas Walzthoeni, Christina Lindner, Anika Boettcher, Silke S. Heinzmann, Andreas Israel, Ahmed Elagamy Mohamed Mahmoud Khalil, Alexander Braun, Ines Pramme-Steinwachs, Ingo Burtscher, Philippe Schmitt-Kopplin, Matthias Heinig, Martin Elsner, Heiko Lickert, Fabian J. Theis, Siegfried Ussar
Summary: Research identified a subset of adolescent preadipocytes expressing the mature white adipocyte marker Asc-1 that showed a low ability to differentiate into beige adipocytes compared to Asc-1 negative cells in vitro. Loss of Asc-1 in subcutaneous preadipocytes resulted in spontaneous differentiation of beige adipocytes in vitro and in vivo.
NATURE COMMUNICATIONS
(2021)