Article
Biochemistry & Molecular Biology
Muheeb Beg, Wei Zhang, Andrew C. McCourt, Sven Enerback
Summary: This study uncovered a new mechanism where activation of lipolysis leads to degradation of TXNIP and increased glucose uptake, mediated largely by ATGL in adipocytes. TXNIP degradation upon cAMP signaling enhances glucose uptake and lactate production, showing a crosstalk between lipolysis and glucose metabolism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Seong J. An, Felix Rivera-Molina, Alexander Anneken, Zhiqun Xi, Brian McNellis, Vladimir Polejaev, Derek Toomre
Summary: The authors study the interplay between vesicle tethering and fusion using optogenetic approaches, finding that tethering regulates the mode of fusion. They demonstrate that active coupling between tethering and fusion is critical for robust membrane merger, with full fusion triggering lamellipodial expansion.
NATURE COMMUNICATIONS
(2021)
Article
Geriatrics & Gerontology
Nadeeja Wijesekara, Rosemary Ahrens, Ling Wu, Tammy Langman, Anurag Tandon, Paul E. E. Fraser
Summary: The study reveals that alpha-syn plays a crucial role in modulating pancreatic beta cell function and glucose transport in peripheral tissues, contributing to the maintenance of normal glucose homeostasis.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Cell Biology
Ariane Schleinitz, Lara-Alina Poettgen, Tal Keren-Kaplan, Jing Pu, Paul Saftig, Juan S. Bonifacino, Albert Haas, Andreas Jeschke
Summary: This study biochemically characterized endosomes purified from untreated or experimentally manipulated cells to establish the functional interdependence of HOPS and interacting proteins. The results showed that Rab2a and Rab7 are present in late endosomes, while Arl8 and BORC are present in lysosomes, and HOPS drives LE-LYS fusion by bridging late endosomal Rab2a with lysosomal BORC-anchored Arl8. Additionally, Rab7 promotes fusion by moving LEs towards LYSs via dynein.
Article
Biology
Jonas R. Knudsen, Kaspar W. Persson, Carlos Henriquez-Olguin, Zhencheng Li, Nicolas Di Leo, Sofie A. Hesselager, Steffen H. Raun, Janne R. Hingst, Raphael Trouillon, Martin Wohlwend, Jorgen F. P. Wojtaszewski, Martin A. M. Gijs, Thomas Elbenhardt Jensen, Michael Czech
Summary: Microtubules play a crucial role in GLUT4 trafficking and maintaining an insulin-responsive GLUT4 pool in skeletal muscle fibers. Disruption of microtubules impairs long-range GLUT4 movement and decreases insulin-stimulated glucose uptake. Kinesin-1-mediated trafficking is essential for insulin-stimulated GLUT4 translocation and glucose uptake.
Article
Biochemistry & Molecular Biology
Sang R. Lee, Su Hee Jeong, Moeka Mukae, Kang Joo Jeong, Hyo-Jung Kwun, Eui-Ju Hong
Summary: This study establishes a moderate-obese diabetes model by inducing GLUT4 degradation with a chemical called GFI. GFI treatment leads to reduced GLUT4 levels, resulting in features of high blood glucose, insulin resistance, and decreased intracellular lipid accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Liang Cui, Ruixiao Peng, Chaofei Zeng, Jialu Zhang, Yinzhu Lu, Lin Zhu, Mengjiao Huang, Qinghua Tian, Yanling Song, Chaoyong Yang
Summary: In this study, a general strategy for in situ detection of tumor-derived extracellular vesicle miRNAs was developed using aptamer-mediated selective fusion. This method offers high selectivity, rapidity, and general applicability, and enables sensitive and selective detection of tumor-derived EV miRNAs using conventional flow cytometry without the need for lysis.
Article
Biology
Priyadarshini Ravindran, Andreas W. Pueschel
Summary: Cdc42 is a crucial regulator of cell polarity and exocytosis, with its isoform Cdc42b playing a specific role in neurons by regulating exocytosis and axon formation.
LIFE SCIENCE ALLIANCE
(2022)
Article
Multidisciplinary Sciences
Shen Zhang, Mindan Tong, Denghao Zheng, Huiying Huang, Linsen Li, Christian Ungermann, Yi Pan, Hanyan Luo, Ming Lei, Zaiming Tang, Wan Fu, She Chen, Xiaoxia Liu, Qing Zhong
Summary: In this study, the authors biochemically reconstitute the mammalian HOPS complex in proteoliposomes and propose a model of complex assembly that depends on Rab2 and Rab39A.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Dougall Norris, Pengyi Yang, Sung-Young Shin, Alison L. Kearney, Hani Jieun Kim, Thomas Geddes, Alistair M. Senior, Daniel J. Fazakerley, Lan K. Nguyen, David E. James, James G. Burchfield
Summary: The study found that intercellular heterogeneity in insulin signaling primarily stems from variance in gene/protein expression between cells rather than within cells. This single-cell heterogeneity leads to differences in population response to insulin signaling.
Article
Multidisciplinary Sciences
Hong Huang, Qinqin Ouyang, Min Zhu, Haijia Yu, Kunrong Mei, Rong Liu
Summary: The study reveals that mTORC1 inhibits SNARE complex formation by phosphorylating VAMP8, thus blocking autophagosome-lysosome fusion. SCFD1 is identified as a crucial protein in autolysosomes required for SNARE complex formation and autophagosome-lysosome fusion.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Zhao Zhang, Zhaomeng Feng, Xiaowei Zhao, Dominique Jean, Zhiheng Yu, Edwin R. Chapman
Summary: Researchers utilize DNA nanostructures to coat, cluster, and pattern sub-100nm liposomes, creating controlled vesicle networks, strings, and dimers. The DNA coating allows for protein attachment to liposomes and temporal control of membrane fusion through SNARE protein complexes. This convenient and versatile method is highly relevant to bottom-up biology and targeted delivery.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Man Piu Chan, Nobuyuki Takenaka, Takaya Satoh
Summary: Insulin-stimulated glucose uptake in skeletal muscle is impaired in Lep(ob/ob) mouse model due to the complete inhibition of Akt2-mediated activation of Rac1 and partial inhibition of RalA activation downstream of Rac1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Chun-Chieh Huang, Miya Kang, Sajjad Shirazi, Yu Lu, Lyndon F. Cooper, Praveen Gajendrareddy, Sriram Ravindran
Summary: This study developed a method to encapsulate MSC-derived EVs in 3D hydrogels for prolonged delivery by leveraging interactions with ECM proteins, showing enhanced bone regeneration in in vivo models. The technology has the potential for controlled delivery of EVs in various regenerative medicine applications.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Zhiwen Zhu, Dongdong Li, Zeran Jia, Wenhao Zhang, Yuling Chen, Ruixue Zhao, Yan-Ping Zhang, Wen-Hong Zhang, Haiteng Deng, Yinqing Li, Wei Li, Shouhong Guang, Guangshuo Ou
Summary: Eukaryotic organisms adapt to environmental fluctuations by altering their epigenomic landscapes and transcriptional programs. Histone H2Bs are globally degraded in Caenorhabditis elegans during starvation, and the mechanisms underlying this degradation involve ubiquitin and ubiquitin-related enzymes. The retention of aberrant nucleosomal H2Bs leads to abnormal gene expression and impacts animal viability. Furthermore, the ubiquitin-proteasome system regulates chromosomal histone turnover in human cells.
Editorial Material
Biochemistry & Molecular Biology
Bjorn Moren, Karin G. Stenkula
JOURNAL OF LIPID RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Claes Fryklund, Madelene Borg, Tobias Svensson, Sara Schumacher, Florentina Negoita, Bjorn Moren, Karin G. Stenkula
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2020)
Article
Endocrinology & Metabolism
Ann-Ki Palbrink, Franziska Kopietz, Bjoern Moren, Rene In't Zandt, Federico Kalinec, Karin Stenkula, Olga Goransson, Cecilia Holm, Mans Magnusson, Eva Degerman
BMJ OPEN DIABETES RESEARCH & CARE
(2020)
Article
Multidisciplinary Sciences
Catarina Rippe, Bjoern Moren, Li Liu, Karin G. Stenkula, Johan Mustaniemi, Malin Wennstrom, Karl Sward
Summary: The study identified NG2/CSPG4, CD146/MCAM, and VAP1/AOC3 as highly responsive vascular target genes of MRTF-SRF signaling, showing correlation with MYOCD, MRTF-A/MKL1, and SRF across human tissues. Overexpression of MRTFs increased mRNA and protein levels of these genes in cultured human smooth muscle cells, with effects exerted through SRF and involving the chromatin remodeling enzyme KDM3A. These findings suggest a mechanism by which MRTF-SRF signaling regulates the expression of specific genes in vascular cells.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Charlotte Erlanson-Albertsson, Karin G. Stenkula
Summary: Food intake, particularly foods rich in fat, can lead to elevated circulating endotoxins and inflammatory markers postprandially, impacting endotoxemia and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Otorhinolaryngology
Ann-Ki Palbrink, Bjorn Moren, Karin G. Stenkula, Mans Magnusson, Eva Degerman
Summary: The study suggests that insulin induces phosphorylation of Nedd4-2 through the PI3K/PKB signaling pathway. Additionally, certain bioactive molecules such as sphingosine 1-phosphate may protect Nedd4-2 against insulin resistance.
ACTA OTO-LARYNGOLOGICA
(2022)
Article
Physiology
Li Liu, Elisabeth Bankell, Catarina Rippe, Bjorn Moren, Karin G. Stenkula, Bengt-Olof Nilsson, Karl Sward
Summary: Myocardin related transcription factors play a key role in smooth muscle cell differentiation, with both anti-inflammatory and pro-inflammatory properties observed in different cell types. The impact of these factors on inflammatory signaling pathways remains complex and cell-type dependent.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Physiology
Claes Fryklund, Bjorn Moren, Shrenika Shah, Mario Grossi, Eva Degerman, Claudia Matthaeus, Karin G. Stenkula
Summary: The study highlights the importance of EHD2 for lipid metabolism, showing that knockout of EHD2 leads to decreased expression of FABP4 and caveolin-1, as well as reduced lipolysis in adipocytes. These findings emphasize differences in mechanisms regulating lipid handling in adipose tissue depots and suggest that impaired lipolysis in EHD2 knockout primary adipocytes likely occurs at the level of, or downstream of, protein kinase A (PKA).
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Medicine, Research & Experimental
Claes Fryklund, Bjorn Moren, Mathis Neuhaus, Vipul Periwal, Karin G. Stenkula
Summary: Rosiglitazone improves glucose transport in hypertrophic adipocytes without affecting cell size. This improvement is associated with extracellular matrix remodeling and restoration of actin turnover.
Article
Biochemistry & Molecular Biology
Li Liu, Dmytro Kryvokhyzha, Catarina Rippe, Aishwarya Jacob, Andrea Borreguero-Munoz, Karin G. Stenkula, Ola Hansson, Christopher W. J. Smith, Steven A. Fisher, Karl Sward
Summary: The study reveals the association between MYOCD and four splicing factors, which are involved in the control of SMC-specific alternative exon usage and splicing. RNA-sequencing and analysis demonstrate that MYOCD contributes to the regulation of transcript diversity through alternative exon usage and splicing, playing a critical role in SMC differentiation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Karin G. Stenkula, Lisa Esbjornsson Klemendz, Claes Fryklund, Nils Wierup, Wathik Alsalim, Mona Landin-Olsson, Lena Trinh, Sven Mansson, Louise Bennet
Summary: This study suggests that early signs of disturbed fat metabolism in Middle Eastern immigrants without cardiovascular disease risk factors are primarily reflected in elevated postprandial plasma triglyceride levels, rather than differences in fat distribution.
LIPIDS IN HEALTH AND DISEASE
(2022)
Article
Food Science & Technology
Dorota Kotowska, Mathis Neuhaus, Lovisa Heyman-Linden, Bjorn Moren, Shuyi Li, Dmytro Kryvokhyzha, Karin Berger, Karin G. Stenkula
Summary: This study highlights the beneficial effects of a lingonberry supplemented diet in preventing obesity and improving insulin levels, which may be achieved through influencing adipose tissue function.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Cell Biology
Claes Fryklund, Mathis Neuhaus, Bjorn Moren, Andrea Borreguero-Munoz, Richard Lundmark, Karin G. Stenkula
Summary: This study investigates the adipocyte plasticity and systemic insulin sensitivity in response to high-fat diet (HFD) feeding. The results show that there is a metabolic tipping point between 8 and 12 weeks of HFD feeding where the adipocyte size plasticity deteriorates. Additionally, an increased pool of hypertrophic inguinal adipocytes may contribute to aggravated insulin resistance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Christian Simonsson, William Lovfors, Niclas Bergqvist, Elin Nyman, Peter Gennemark, Karin G. Stenkula, Gunnar Cedersund
Summary: Insulin resistance leads to excessive insulin secretion, which can eventually result in beta-cell failure and type 2 diabetes (T2D). In order to study the progression of T2D, a complex disease involving multiple processes on different scales, a mathematical model of mice with insulin resistance was developed. This model successfully explained multi-scale data on insulin resistance in mice and made predictions that were supported by new experimental data. Additionally, the model was extended to simulate scenarios of T2D progression, providing insights and predictions on non-measured processes. Overall, this in silico mouse model is a valuable tool for studying T2D and can help in simulating treatment strategies and translating findings to humans.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Vance Holt, Bjorn Moren, Claes Fryklund, Robert A. Colbert, Karin G. Stenkula
Summary: During inflammation, IFN gamma and TNF can rapidly induce glucose uptake and glycolysis in keratinocytes, which may contribute to inflammation in psoriatic disease.