Article
Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Article
Biochemistry & Molecular Biology
Jeffrey R. van Senten, Thor C. Moller, Ee Von Moo, Sofie D. Seiersen, Hans Brauner-Osborne
Summary: In this study, the contribution of PKC isozymes in the internalization of mGlu5 receptors was investigated using PKC KO cell lines. It was found that both cPKCs and nPKCs are involved in the internalization process and have compensatory functions. Additionally, G??q/11 proteins, GRK2, and AP-2 were identified as mediators of mGlu5 internalization.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jeffrey R. van Senten, Thor C. Moller, Ee Von Moo, Sofie D. Seiersen, Hans Brauner-Osborne
Summary: The study demonstrates the significant role of PKC isozymes in the internalization of mGlu(5) receptors, with both cPKCs and nPKCs compensating for each other. Additionally, G alpha q/11 proteins, GRK2, and AP-2 were found to be involved in the internalization process.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Rawad Hodeify, Maya Dib, Ethel Alcantara-Adap, Raphael Courjaret, Nancy Nader, Cleo Z. Reyes, Ayat S. Hammad, Satanay Hubrack, Fang Yu, Khaled Machaca
Summary: This study elucidates the regulation of Orai1 endocytosis during meiosis by the C-terminal structure, specifically through a region called the C-terminus Internalization Handle (CIH). The strength of intersubunit coiled coil (CC) domains controls access to CIH and ultimately affects Orai1 internalization. The findings suggest that Orai1 endocytosis requires both the N-terminal caveolin binding motif (CBM) and the C-terminal CIH, with access to CIH being influenced by the strength of intersubunit C-terminal CC.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Zhuoming Liu, Laura A. VanBlargan, Louis-Marie Bloyet, Paul W. Rothlauf, Rita E. Chen, Spencer Stumpf, Haiyan Zhao, John M. Errico, Elitza S. Theel, Mariel J. Liebeskind, Brynn Alford, William J. Buchser, Ali H. Ellebedy, Daved H. Fremont, Michael S. Diamond, Sean P. J. Whelan
Summary: The study found that antibodies targeting the SARS-CoV-2 spike protein have escape mutations, different monoclonal antibodies have unique resistance profiles, some mutants are resistant to multiple antibodies while some variants can escape neutralization by convalescent sera. Comparing antibody-mediated mutations with circulating SARS-CoV-2 sequences revealed substitutions that may weaken neutralizing immune responses in some individuals, warranting further investigation.
CELL HOST & MICROBE
(2021)
Article
Biochemistry & Molecular Biology
Elodie Blondel-Tepaz, Marie Leverve, Badr Sokrat, Justine S. Paradis, Milena Kosic, Kusumika Saha, Cedric Auffray, Evelyne Lima-Fernandes, Alessia Zamborlini, Anne Poupon, Louis Gaboury, Jane Findlay, George S. Baillie, Herve Enslen, Michel Bouvier, Stephane Angers, Stefano Marullo, Mark G. H. Scott
Summary: The protein beta-arrestin2 plays a critical role in regulating the Mdm2-p53 signaling axis by promoting the nuclear-cytoplasmic shuttling of Mdm2 and enhancing p53 signaling. While beta-arrestin2 can be SUMOylated, it is the non-covalent interaction between SUMO and beta-arrestin2, mediated by a SUMO interaction motif (SIM), that is essential for its cytonuclear trafficking function. Depletion of the RanBP2/RanGAP1-SUMO nucleocytoplasmic transport hub leads to defective beta-arrestin2 nuclear entry, inhibiting its ability to displace Mdm2 from the nucleus and enhancing p53 signaling in cancer cells.
Article
Cell Biology
Tyler N. Starr, Allison J. Greaney, Adam S. Dingens, Jesse D. Bloom
Summary: The study mapped mutations to the SARS-CoV-2 spike receptor-binding domain that escape binding by certain monoclonal antibodies. These mutations are concentrated in specific lineages of SARS-CoV-2. The authors suggest diversifying the epitopes targeted by antibodies and antibody cocktails to make them more resilient to SARS-CoV-2 antigenic evolution.
CELL REPORTS MEDICINE
(2021)
Article
Cell Biology
Yufeng Luo, Shuo Liu, Jiguo Xue, Ye Yang, Junxuan Zhao, Ying Sun, Bolun Wang, Shenyi Yin, Juan Li, Yuchao Xia, Feixiang Ge, Jiqiao Dong, Lvze Guo, Buqing Ye, Weijin Huang, Youchun Wang, Jianzhong Jeff Xi
Summary: This study established a robust mammalian cell-surface-display platform to study the interactions between SARS-CoV-2 variants, cellular receptor ACE2, and monoclonal antibodies on a large scale. By analyzing the mutational landscape, it was found that certain key mutations in the spike protein can increase infectivity and confer resistance to specific monoclonal antibodies. These methods have significant implications for the precise control of SARS-CoV-2 in the future.
Article
Biochemistry & Molecular Biology
Maria Soledad Alvarez, Estefania Nunez, Marina Fuertes-Agudo, Carme Cucarella, Maria Fernandez-Velasco, Lisardo Bosca, Jesus Vazquez, Rodrigue Rossignol, Paloma Martin-Sanz, Marta Casado
Summary: This study suggests a new association between COX-2 and mitochondria, which may contribute to increased expression of respiratory chain complex IV proteins, enhanced respiratory capacity, and increased ATP levels in the hearts of COX-2 transgenic mice, thus providing protective effects against ischemia-reperfusion injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Tara C. Marcink, Gillian Zipursky, Wenjing Cheng, Kyle Stearns, Shari Stenglein, Kate Golub, Frances Cohen, Francesca Bovier, Daniel Pfalmer, Alexander L. Greninger, Matteo Porotto, Amedee des Georges, Anne Moscona
Summary: This study used cryo-electron tomography to visualize the fusion complex of human parainfluenza virus 3, revealing the mechanism of fusion and potential druggable targets for paramyxoviruses.
Article
Multidisciplinary Sciences
Yunlong Cao, Ayijiang Yisimayi, Fanchong Jian, Weiliang Song, Tianhe Xiao, Lei Wang, Shuo Du, Jing Wang, Qianqian Li, Xiaosu Chen, Yuanling Yu, Peng Wang, Zhiying Zhang, Pulan Liu, Ran An, Xiaohua Hao, Yao Wang, Rui Feng, Haiyan Sun, Lijuan Zhao, Wen Zhang, Dong Zhao, Jiang Zheng, Lingling Yu, Can Li, Na Zhang, Rui Wang, Xiao Niu, Sijie Yang, Xuetao Song, Yangyang Chai, Ye Hu, Yansong Shi, Linlin Zheng, Zhiqiang Li, Qingqing Gu, Fei Shao, Weijin Huang, Ronghua Jin, Zhongyang Shen, Youchun Wang, Xiangxi Wang, Junyu Xiao, Xiaoliang Sunney Xie
Summary: Omicron sublineages BA.2.12.1, BA.4 and BA.5 have higher transmissibility and increased evasion of neutralizing antibodies compared to the BA.2 lineage. They exhibit similar binding affinities to the ACE2 receptor as BA.2. BA.1 infection after vaccination boosts humoral immune memory against wild-type SARS-CoV-2, but these antibodies are largely evaded by BA.2 and BA.4/BA.5 variants.
Article
Biochemistry & Molecular Biology
Minsoo Kim, John Swenson, Fionn McLoughlin, Elizabeth Vierling
Summary: Studies revealed that heat shock protein 101 (HSP101) is essential for thermotolerance in plants, bacteria, and yeast. Through a suppressor screen in Arabidopsis thaliana, a mutation in the CLEAVAGE STIMULATION FACTOR77 (CstF77) gene was identified as a suppressor of hot1-4, suggesting its critical role in mRNA 3' end maturation. The dominant role of HSP101 in severe heat stress outcome was also demonstrated.
Article
Medicine, General & Internal
V. Shinde, S. Bhikha, Z. Hoosain, M. Archary, Q. Bhorat, L. Fairlie, U. Lalloo, M. S. L. Masilela, D. Moodley, S. Hanley, L. Fouche, C. Louw, M. Tameris, N. Singh, A. Goga, K. Dheda, C. Grobbelaar, G. Kruger, N. Carrim-Ganey, V. Baillie, T. de Oliveira, A. Lombard Koen, J. J. Lombaard, R. Mngqibisa, A. E. Bhorat, G. Benade, N. Lalloo, A. Pitsi, P. -L. Vollgraaff, A. Luabeya, A. Esmail, F. G. Petrick, A. Oommen-Jose, S. Foulkes, K. Ahmed, A. Thombrayil, L. Fries, S. Cloney-Clark, M. Zhu, C. Bennett, G. Albert, E. Faust, J. S. Plested, A. Robertson, S. Neal, I. Cho, G. M. Glenn, F. Dubovsky, S. A. Madhi
Summary: The NVX-CoV2373 vaccine showed efficacy in preventing Covid-19, with higher vaccine efficacy observed among HIV-negative participants. Most infections were caused by the B.1.351 variant.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
John Janetzko, Ryoji Kise, Benjamin Barsi-Rhyne, Dirk H. Siepe, Franziska M. Heydenreich, Kouki Kawakami, Matthieu Masureel, Shoji Maeda, K. Christopher Garcia, Mark von Zastrow, Asuka Inoue, Brian K. Kobilka
Summary: This study reveals the role of membrane phosphoinositides (PIPs) in β-arrestin recruitment and GPCR-β-arrestin complex dynamics through cell-based and in vitro biophysical assays. GPCRs are classified into two groups, with one requiring PIP binding for β-arrestin recruitment and the other not. Plasma membrane PIPs enhance the active conformation of β-arrestin and stabilize GPCR-β-arrestin complexes by promoting a fully engaged state. As allosteric modulators of GPCR-β-arrestin complex dynamics, membrane PIPs allow for additional conformational diversity beyond GPCR phosphorylation alone. For GPCRs that require membrane PIP binding for β-arrestin recruitment, this provides a mechanism for rapid β-arrestin release upon GPCR translocation to endosomes, enabling its quick recycling.
Article
Biochemistry & Molecular Biology
Rita E. Chen, Xianwen Zhang, James Brett Case, Emma S. Winkler, Yang Liu, Laura A. VanBlargan, Jianying Liu, John M. Errico, Xuping Xie, Naveenchandra Suryadevara, Pavlo Gilchuk, Seth J. Zost, Stephen Tahan, Lindsay Droit, Jackson S. Turner, Wooseob Kim, Aaron J. Schmitz, Mahima Thapa, David Wang, Adrianus C. M. Boon, Rachel M. Presti, Jane A. O'Halloran, Alfred H. J. Kim, Parakkal Deepak, Dora Pinto, Daved H. Fremont, James E. Crowe, Davide Corti, Herbert W. Virgin, Ali H. Ellebedy, Pei-Yong Shi, Michael S. Diamond
Summary: The study analyzed antibody neutralization activity against a panel of authentic isolates and chimeric SARS-CoV-2 variants, showing significantly reduced neutralizing activity against the B.1.351 variant first identified in South Africa. Antibodies targeting the receptor-binding domain and N-terminal domain, monoclonal antibodies, convalescent sera, and mRNA vaccine-induced immune sera exhibited decreased inhibitory activity against viruses with an E484K spike mutation, suggesting a need for updated monoclonal antibodies or vaccine adjustments to prevent loss of protection against emerging variants.
Article
Cell Biology
Shanna Lynn Bowman, Daniel John Shiwarski, Manojkumar A. Puthenveedu
JOURNAL OF CELL BIOLOGY
(2016)
Article
Cell Biology
Daniel J. Shiwarski, Marlena Darr, Cheryl A. Telmer, Marcel P. Bruchez, Manojkumar A. Puthenveedu
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Pharmacology & Pharmacy
Zara Y. Weinberg, Amanda S. Zajac, Tiffany Phan, Daniel J. Shiwarski, Manojkumar A. Puthenveedu
MOLECULAR PHARMACOLOGY
(2017)
Review
Cell Biology
Zara Y. Weinberg, Manojkumar A. Puthenveedu
Review
Cell Biology
Zara Y. Weinberg, Stephanie E. Crilly, Manojkumar A. Puthenveedu
CURRENT OPINION IN CELL BIOLOGY
(2019)
Article
Pharmacology & Pharmacy
Jennifer M. Kunselman, Amanda S. Zajac, Zara Y. Weinberg, Manojkumar A. Puthenveedu
MOLECULAR PHARMACOLOGY
(2019)
Article
Pharmacology & Pharmacy
Renee A. Bouley, Zara Y. Weinberg, Helen Waldschmidt, Yu-Chen Yen, Scott D. Larsen, Manojkumar A. Puthenveedu, John J. G. Tesmer
MOLECULAR PHARMACOLOGY
(2020)
Editorial Material
Pharmacology & Pharmacy
Manojkumar A. Puthenveedu
MOLECULAR PHARMACOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Stephanie E. Crilly, Manojkumar A. Puthenveedu
Summary: GPCRs are integral membrane proteins that transduce signals from both the cell surface and intracellular membrane compartments, producing functional effects that differ from canonical plasma membrane signaling. The activity and signaling of GPCRs are influenced by intracellular membranes and their associated lipids and proteins, resulting in a spatial bias in GPCR signaling.
JOURNAL OF MEMBRANE BIOLOGY
(2021)
Article
Biology
Jennifer M. Kunselman, Achla Gupta, Ivone Gomes, Lakshmi A. Devi, Manojkumar A. Puthenveedu
Summary: The study shows that while multiple physiological agonists can activate the same receptor, it may not necessarily be true redundancy, but rather a way to regulate downstream signaling specificity by controlling the spatiotemporal profile of signaling molecules.
Article
Biology
Stephanie E. Crilly, Wooree Ko, Zara Y. Weinberg, Manojkumar A. Puthenveedu
Summary: The study reveals that the same ligand can activate different active states of G protein-coupled receptors (GPCRs) in distinct cellular environments, allowing for coupling to different effectors. By investigating Golgi and surface pools, researchers can understand the receptor's different responses to drugs in different subcellular locations.
Article
Pharmacology & Pharmacy
Joshua Lott, Emily M. Jutkiewicz, Manojkumar A. Puthenveedu
Summary: The synthetic cannabinoid WIN55,212-2 (WIN) disrupts the Golgi apparatus and microtubule network in cells, independent of cannabinoid receptors. This unique effect may contribute to the physiological and therapeutic effects of WIN.
MOLECULAR PHARMACOLOGY
(2022)
Article
Neurosciences
G. Aditya Kumar, Manojkumar A. Puthenveedu
Summary: This article discusses the common mechanisms of GPCR family members in responding to neurotransmitters in the brain as well as their diversity in terms of intracellular location, interacting partners, effectors, and signaling consequences. The interactions of these factors may generate specific spatiotemporal patterns of GPCR signaling in cells.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Editorial Material
Pharmacology & Pharmacy
Ian B. Chronis, Manojkumar A. Puthenveedu
Summary: Tolerance poses a challenge to the safe use of opioids as painkillers, but the mechanisms behind tolerance have been unclear. A recent study by Maza and colleagues identifies PTCHD1 protein and its impact on cellular cholesterol as potential targets for preventing opioid tolerance.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Cell Biology
Hao Chen, Zara Y. Weinberg, G. Aditya Kumar, Manojkumar A. Puthenveedu
Summary: Chen et al. discover that VAMP2 is selectively packaged with μ opioid receptors into vesicles and mediates their fusion. This selective packaging allows for individual regulation of GPCR trafficking. This finding provides insight into the fusion machinery responsible for GPCR delivery.
JOURNAL OF CELL BIOLOGY
(2023)