期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 284, 期 41, 页码 27974-27979出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M109.037929
关键词
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资金
- CNRS
- European Union 6th Framework Programme TRANS-REG [LSHG-CT-2004-502950]
- SIROCCO [LSHG-CT-2006-037900]
By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1 alpha (HP1 alpha) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1 alpha-interacting molecule. More importantly, hnRNP U depletion reduces HP1 alpha-dependent gene silencing and disturbs HP1 alpha subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1 alpha function, shedding new light on the mode of action of HP1 alpha and on the function of hnRNP U.
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