Article
Multidisciplinary Sciences
Roman Stavniichuk, Ann DeLaForest, Cayla A. Thompson, James Miller, Rhonda F. Souza, Michele A. Battle
Summary: The study showed the critical role of GATA4 in determining the fate of columnar epithelial cells during gastric and esophageal development. In diseases like Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC), up-regulation of GATA4 expression is associated with poor patient outcomes, and its ectopic expression suppresses squamous cell marker gene expression. This suggests that GATA4 may play a repressive role in BE and EAC by inhibiting genes marking the stratified squamous epithelial cell lineage.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Zhong-xin Jiang, Yi-ning Wang, Zi-yuan Li, Zhi-hui Dai, Yi He, Kun Chu, Jia-yi Gu, Yi-Xuan Ji, Ning-xia Sun, Fu Yang, Wen Li
Summary: The study demonstrates that m6A adenine regulates the function of GCs in female ovarian aging by modulating FTO demethylase, which in turn affects ovarian aging. FTO retards ovarian cell aging by regulating FOS expression.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Yamei Chen, Shihao Zhu, Tianyuan Liu, Shaosen Zhang, Junting Lu, Wenyi Fan, Lin Lin, Tao Xiang, Jie Yang, Xuan Zhao, Yiyi Xi, Yuling Ma, Guoyu Cheng, Dongxin Lin, Chen Wu
Summary: In this study, a comprehensive analysis of 79 multistage esophageal lesions from 29 ESCC patients was conducted using single-cell RNA sequencing and spatial transcriptomics. The results showed a gradual loss of ANXA1 expression in epithelial cells during lesion progression, which was attributed to the suppression of its transcription factor KLF4. It was also found that ANXA1 is a ligand for fibroblasts and loss of ANXA1 leads to uncontrolled transformation of normal fibroblasts into cancer-associated fibroblasts (CAFs), facilitated by TGF-b secreted by malignant epithelial cells. This highlights the importance of ANXA1/FPR2 signaling in the crosstalk between epithelial cells and fibroblasts in promoting ESCC.
Article
Multidisciplinary Sciences
Yuko Hasegawa, Kevin Struhl
Summary: The transcription factor SP1 exhibits varying binding dynamics at different target sites in the human genome, potentially influenced by factors such as location and cobinding factors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Physiology
Luis Rodrigo Cataldo, Tania Singh, Kavya Achanta, Sara Bsharat, Rashmi B. Prasad, Cheng Luan, Erik Renstrom, Lena Eliasson, Isabella Artner
Summary: This study found that the loss of MAFA and MAFB in human beta cells contributes to impaired insulin exocytosis by regulating genes involved in exocytosis. The downregulation of exocytosis-related genes was observed in MAFA-deficient cells, and the expression of these genes correlated positively with MAFA and MAFB in human islets. The silencing of MAFA and MAFB resulted in impaired insulin secretion and reduced expression of certain exocytosis-related genes.
Article
Cell Biology
Youwei Chen, Ying Chen, Qianyan Li, Huahua Liu, Jiazhen Han, Hailin Zhang, Liming Cheng, Gufa Lin
Summary: This study identifies the presence of short C-terminal MSI1 (MSI1-C) proteins in early mouse embryos and mouse ESCs, but not in human ESCs. MSI1-C plays an essential role in regulating pluripotency states and early embryonic development by binding to RNAs involved in DNA-damage repair, enhancing the survival and blastoid formation ability of hESCs.
Article
Multidisciplinary Sciences
Ra Ham Lee, Jae-Don Oh, Jae Sam Hwang, Hak-Kyo Lee, Donghyun Shin
Summary: This study identified the insect-derived peptide PCC-1 as a potential anticancer agent for melanoma, demonstrating its effects on antiproliferation, apoptosis, and cell cycle arrest in melanoma cells. The research also revealed a novel anticancer mechanism through the regulation of the transcription factor Sp1 protein by insect-derived peptides.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Ying Pan, Xue Wang, Yang Li, Peiyu Yan, Hong Zhang
Summary: This study reveals that hUMSCs-derived exosomal miR-503-3p inhibits the development of endometrial cancer by suppressing MEST. This finding has significant implications for the treatment of endometrial cancer.
CANCER GENE THERAPY
(2022)
Article
Multidisciplinary Sciences
Sierra M. Collins, Katherine A. Alexander, Stefan Lundh, Alexander J. Dimitri, Zhen Zhang, Charly R. Good, Joseph A. Fraietta, Shelley L. Berger
Summary: Chimeric antigen receptor (CAR) T cell therapy is hindered by T cell exhaustion (T-EX), but loss of TET2 gene enhances central memory T cell differentiation and antitumor efficacy. TET2 deficiency increases chromatin accessibility and expression of TOX2, an exhaustion regulator. Knockdown of TOX increases T-CM percentage, while knockdown of TOX2 decreases T-CM percentage and proliferation. TOX2 binds to promoters of T-CM genes, suggesting its role in CAR T cell therapy via modulated TOX2 expression.
Article
Multidisciplinary Sciences
Viviana Cobos Jimenez, Aviva Geretz, Andrey Tokarev, Philip K. Ehrenberg, Selase Deletsu, Kawthar Machmach, Prakriti Mudvari, J. Natalie Howard, Amanda Zelkoski, Dominic Paquin-Proulx, Gregory Q. Del Prete, Caroline Subra, Eli A. Boritz, Alberto Bosque, Rasmi Thomas, Diane L. Bolton
Summary: AP-1 transcription factor plays an important role in latent HIV-1 infection and reactivation. Inhibiting AP-1 may be a potential therapeutic approach to limit HIV-1 reservoirs.
Article
Biochemistry & Molecular Biology
Rui Fu, Kimberly Wellman, Amber Baldwin, Juilee Rege, Kathryn Walters, Antje Hirsekorn, Kent Riemondy, William E. Rainey, Neelanjan Mukherjee
Summary: The study identified the involvement of RNA-binding proteins and RNA decay in the kinetics of AngII-induced gene expression, which coordinated an increase in both synthesis and decay. This facilitated the major transcriptomic changes required to prevent aldosterone overproduction.
Article
Multidisciplinary Sciences
Nicole M. Wong, Elizabeth Frias, Frederic D. Sigoillot, Justin H. Letendre, Marc Hild, Wilson W. Wong
Summary: Cell-based transcriptional reporters are crucial in high-throughput screening, but many of them have weak or transient responses. This article introduces a digitizer circuit for amplifying reporter activity, increasing sensitivity, and retaining memory of pathway activation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Ching- Chang, Chuen-Mao Yang
Summary: 15d-PGJ(2) induces the expression of HO-1 in bEnd.3 cells through the activation of NOX- and mitochondria-derived ROS-dependent PKC delta/JNK1/2/Sp1/AP-1 signaling pathway, thus protecting against inflammatory responses.
Article
Chemistry, Multidisciplinary
Mustapha Najih, Audrey Basque, Ha Tuyen Nguyen, Mariama Diawara, Luc J. Martin
Summary: Gap junctions, mainly formed by Gja1, are important in the regulation of spermatogonia proliferation and differentiation. The expression of Gja1 is regulated through various processes, including gene transcription, mRNA maturation, protein synthesis, post-translational modifications, plasma membrane integration, and protein degradation. Abnormal decrease in Gja1 expression is observed in testicular germ cell tumors, suggesting a loss of cooperation between AP-1 factors. Jun and Fosl2 cooperate to activate the Gja1 promoter, and protein kinase A and calcium-calmodulin protein kinase I improve the cooperation between AP-1 factors.
APPLIED SCIENCES-BASEL
(2022)
Article
Plant Sciences
Zhen Wang, Zai Zheng, Yumin Zhu, Shuyao Kong, Dong Liu
Summary: To understand the molecular mechanism of plant transcriptional responses to phosphate (Pi) starvation, this study investigated the function of PHR1 and its homologs PHL1, PHL2, and PHL3 in Arabidopsis. The results showed that PHR1/PHL1 and PHL2/PHL3 function as two separate modules in regulating plant development and transcriptional responses to Pi starvation. PHR1 plays a dominant role in the PHR1/PHL1 module, while PHL2 and PHL3 contribute similarly in the PHL2/PHL3 module. The study also identified the common and specific targets of these PHR proteins and revealed their role as both positive and negative regulators of Pi starvation-responsive gene expression.