Article
Cell Biology
Nicolas Rochereau, Eva Michaud, Louis Waeckel, Martin Killian, Remi Gayet, Roman Goguyer-Deschaumes, Xavier Roblin, Gilles Biolley, Blaise Corthesy, Stephane Paul
Summary: Recent studies have shown that SIgM can also undergo retrotranscytosis through M cells, indicating a potential role in regulating mucosal immunity.
Article
Immunology
Albert Bondt, Kelly A. Dingess, Max Hoek, Danique M. H. van Rijswijck, Albert J. R. Heck
Summary: The study shows that the total pool of sIgA1 in milk is dominated by a small number of clones, with little overlap between donors and minimal changes over time.
Mothers provide protection to newborns through transfer of antibodies via breastfeeding.
The new method can visualize the transfer of immunoglobulin clonal repertoires from mother to infant and monitor changes over time in response to maternal physiology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Sarah M. Mahdally, Mariana Izquierdo, Rose M. Viscardi, Laurence S. Magder, Helena M. Crowley, Andrea C. Bafford, Cinthia B. Drachenberg, Mauricio J. Farfan, Alessio Fasano, Marcelo B. Sztein, Rosangela Salerno-Goncalves
Summary: Mahdally et al. demonstrated that Secretory-IgA binding to intestinal microbiota attenuates inflammatory reactions in preterm infants, and this attenuation is dependent on the changes in microbiota as the intestinal barrier matures. The study found a significant correlation between Secretory-IgA affinity to the microbiota and the maturity of the infant's intestinal barrier. However, Secretory-IgA affinity was not associated with the development of host defenses, but rather depended on microbiota shifts.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mark D. Owen, Charlotte Sacks, Siva Bathina, Robyn A. Emmins, Alan J. Dickson
Summary: Improved understanding of expression of recombinant immunoglobulin (IgG)-based therapies can decrease manufacturing process costs and bring down costs to patients. Deletion of C-terminal Lysine (C-Lys) from IgG molecules has been shown to greatly impact yield. This study set out to characterise structural components of IgG C-terminal variants which modulate protein expression by examination of the consequences of mutations at the C-terminal of IgG on expression and by the use of fluorescent C-terminal fragment fusion proteins.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Immunology
Christine Baksmeier, Pat Blundell, Julia Steckel, Verena Schultz, Quan Gu, Ana Da Silva Filipe, Alain Kohl, Chris Linnington, Dongli Lu, Anne Dell, Stuart Haslam, Jiabin Wang, Dan Czajkowsky, Norbert Goebels, Richard J. Pleass
Summary: IVIG is an established treatment for autoimmune conditions, but mechanisms of action remain unclear. This study used adapted Fc fragments to dissect IVIG effector mechanisms in an ex vivo model of demyelination. The protective effects of the adapted Fc fragments were dose-dependent and attributed to interference with complement-mediated damage to oligodendrocytes. Overall, findings suggest that recombinant biomimetics may be more effective than IVIG in controlling demyelination.
Article
Agriculture, Dairy & Animal Science
Axelle Collinet, Pauline Grimm, Emmanuel Jacotot, Veronique Julliand
Summary: This study investigated the use of blood and fecal biomarkers as non-invasive indicators of mucosal integrity loss and local inflammation associated with microbiota disturbances in the large intestine of horses. The results showed significant correlations between the biomarkers and bacterial variations in the feces, supporting the hypothesis of a relationship between microbiota disturbances and intestinal barrier disruption in horses.
JOURNAL OF ANIMAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Raul Argueello-Garcia, Julio Cesar Carrero, M. Guadalupe Ortega-Pierres
Summary: Intestinal diseases caused by protistan parasites, including Giardia, Entamoeba, Cryptosporidium, and Blastocystis, are a significant burden globally due to their severe impact on diarrhea and inflammation. These parasites interact with epithelial cells and release virulence markers, such as peptide hydrolases, which affect host tissues and immune responses, leading to increased pathogenicity. Targeting these enzymes may be a potential approach for drug development, with several promising results obtained in experimental models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jesus S. Aguilar Diaz de Leon, Isaac Aguilar, Adam W. Barb
Summary: N-glycan processing plays an important role in regulating macrophage effector function in antibody-mediated therapies. Inhibition of N-glycan processing by kifunensine enhances target binding and phagocytosis of antibody-coated target cells by macrophages. CD64, CD32, and CD16 are the crucial Fcγ receptors involved in macrophage ADCP. Differential phosphorylation levels of immune receptors correlate with increased ADCP.
Article
Biochemical Research Methods
Wei Wang, Qing Chen
Summary: Fc gamma R binding characterization is critical during therapeutic antibody development. A new assay format using Surface plasmon resonance (SPR) was developed to characterize IgG-Fc gamma R interaction in the presence of antigen. The study provides evidence of antigen binding facilitating IgG-Fc gamma R interaction, especially for huIgG2. Evaluating the IgG-Fc gamma R interaction in the presence of antigen may help design safer and more effective biotherapeutics.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Hongyu Lu, Youmei Jin, Hao Yang, Ze Tao, Jie Chen, Shiyuan Chen, Yanru Feng, Huimin Xing, Xiaofeng Lu
Summary: Fragment antigen-binding (Fab) has several advantages in the treatment and diagnosis of diseases. However, the purification of Fab faces challenges. In this study, a self-trimerizing domain (tri) was fused to C-Fab to increase its affinity. C-Fab-tri showed promising binding to Fab from various species and outperformed protein G and protein L in terms of purification efficiency and specificity.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Immunology
Xuerun Liu, Luoyang Wang, Huiren Zhuang, Zhenghuan Yang, Guoqiang Jiang, Zheng Liu
Summary: Recent research has shown that anthocyanins have prebiotic attributes and can be used to treat microbiota-associated diseases. This study investigates the effects of bilberry anthocyanins on the gut microbiota composition, metabolism, and the intestinal mucosal immune system of mice. The findings suggest that oral administration of anthocyanins can help maintain intestinal homeostasis and may have applications in immunotherapy and related fields.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Algirdas Grevys, Rahel Frick, Simone Mester, Karine Flem-Karlsen, Jeannette Nilsen, Stian Foss, Kine Marita Knudsen Sand, Thomas Emrich, Jens Andre Alexander Fischer, Victor Greiff, Inger Sandlie, Tilman Schlothauer, Jan Terje Andersen
Summary: The variable region sequences of IgG antibodies significantly impact their cellular uptake, recycling, and degradation, as well as their binding to FcRn. Differences in charge patch also modulate binding and transport properties. Fc-engineering may improve FcRn binding and overcome unfavorable charge patches for longer plasma half-life.
Article
Biochemistry & Molecular Biology
Katsuhiro Nakanishi, Noriko Mogi, Yuki Kikuchi, Minami Matsuda, Takeshi Matsuoka, Kotome Shiina, Shota Morikane, Kohta Kurohane, Yasuo Niwa, Hirokazu Kobayashi, Yasuyuki Imai
Summary: The study demonstrates that plant-derived secretory component can enhance protease-resistance of SIgA, potentially contributing to its oral application. The reconstituted SIgA with plant-derived SC showed increased resistance to pepsin and trypsin, suggesting a promising alternative for efficient production of orally applicable SIgA.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Agnieszka Zak-Bochenek, Joanna Bajzert, Dominika Sambor, Natalia Siwinska, Bogumila Szponar, Lukasz Laczmanski, Paulina Zebrowska, Aleksandra Czajkowska, Maciej Karczewski, Anna Chelmonska-Soyta
Summary: The defense effect of the gastrointestinal mucosa in horses is influenced by various factors, including the composition and metabolic activity of the microbiome, as well as the presence of parasites. Microbiome diversity is correlated with the production of immunoglobulin A, and a specific bacteria phylum in horses is negatively correlated with fecal egg count.
Article
Biochemistry & Molecular Biology
Claudio Tabolacci, Martina Cordella, Sabrina Mariotti, Stefania Rossi, Cinzia Senatore, Carla Lintas, Lauretta Levati, Daniela D'Arcangelo, Antonio Facchiano, Stefania D'Atri, Roberto Nisini, Francesco Facchiano
Summary: The study focused on secretome profiling of melanoma cells sensitive or resistant to the BRAFi vemurafenib, finding that vemurafenib-resistant melanoma cells can influence dendritic cell maturation and produce higher levels of pro-inflammatory cytokines. Proteomic and cytokine/chemokine secretion analyses suggest a possible involvement of the proteasome pathway in BRAFi resistance, with BRAFi-resistant cells displaying a more aggressive phenotype and increased production of specific proteins. Plasma levels of CD147/basigin and MMP-2 were observed to increase in melanoma patients at the time of treatment failure, supporting the hypothesis that CD147/basigin might play a role in BRAFi resistance.
