Article
Biology
Anna Aakula, Mukund Sharma, Francesco Tabaro, Reetta Natkin, Jesse Kamila, Henrik Honkanen, Matthieu Schapira, Cheryl Arrowsmith, Matti Nykter, Jukka Westermarck
Summary: RAS-mediated human cell transformation requires inhibition of PP2A, and the co-regulated phosphosites on proteins involved in epigenetic gene regulation were discovered in this study. RAS and PP2A co-regulate phosphorylation sites on HDAC1/2, KDM1A, MTA1/2, RNF168, and TP53BP1. The study validates the regulation of HDAC1/2 chromatin recruitment, RNF168-TP53BP1 interaction, and gene expression by RAS and PP2A. This work provides insights into the convergence of RAS and PP2A in cancer.
LIFE SCIENCE ALLIANCE
(2023)
Article
Fisheries
Cheng-dong Jin, Mo-fei Li
Summary: The study demonstrates that Omp1Et is a surface-located virulence factor of E. tarda, and recombinant Omp1Et can effectively induce immune protection as a subunit vaccine.
Review
Immunology
Afreen Shadan, Avik Pathak, Ying Ma, Ranjana Pathania, Rajnish Prakash Singh
Summary: This article reviews the mechanisms, virulence factors, and other factors associated with the pathogenesis of A. baumannii infection. Additionally, it discusses the role of innate and adaptive immune response, as well as the current progress in developing innovative strategies to combat this multidrug-resistant pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Veronika M. Shoba, Dhanushka N. P. Munkanatta Godage, Santosh K. Chaudhary, Arghya Deb, Sachini U. Siriwardena, Amit Choudhary
Summary: Phosphorylation-inducing chimeric small molecules (PHICS) can enable protein kinase C (PKC) to have new activities, such as inducing inhibitory neo-phosphorylations on a variant and phosphorylating cytosol-based neo-substrates. PKC with PHICS can also induce death of chronic myeloid leukemia cells. Synthetic chemistry has the power to expand the chemical and functional diversity of proteins in cells using bifunctional molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biophysics
James E. Kent, Lynn M. Fujimoto, Kyungsoo Shin, Chandan Singh, Yong Yao, Sang Ho Park, Stanley J. Opella, Gregory Plano, Francesca M. Marassi
Summary: By expressing the Ail protein in Escherichia coli, researchers have successfully studied the virulence, cosedimentation, and pellicle formation phenotypes of Yersinia pestis. Additionally, using isolated bacterial cell envelopes combined with high-resolution solid-state NMR spectra, they have revealed the structure of Ail in the bacterial membrane environment and its interactions with human serum components.
BIOPHYSICAL JOURNAL
(2021)
Article
Microbiology
Qun Gao, Shuwei Lu, Mingshu Wang, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Di Sun, Bin Tian, Anchun Cheng
Summary: The study confirmed that the B739_0832 protein contributes to the virulence of Riemerella anatipestifer CH-1 by affecting the survival rate of infected ducklings and the bacterial load in various tissues. Deleting B739_0832 resulted in a higher survival rate and reduced bacterial load, indicating its importance in the pathogenesis of the bacterium.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Kiran Kumari, Parva Kumar Sharma, Yogender Aggarwal, Rajnish Prakash Singh
Summary: In this study, the secreted proteins of Enterobacter sp. S-33 were characterized using mass spectrometry and bioinformatics. It was found that these proteins included various virulence factors and may play a role in the pathogenic mechanisms of bacteria.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Ning Lu, Sarah Kavianpour, Tao Zhang, Xumei Zhang, Dao Nguyen, Ravi Thombre, Lu He, Jiou Wang
Summary: This study identifies MARK2 as a direct kinase of eIF2α in response to proteotoxic stress, and MARK2 itself is regulated by PKCδ. Both MARK2 and PKCδ are activated via phosphorylation in cellular proteotoxic stress responses and human diseases, suggesting a critical role in these processes.
Article
Biochemistry & Molecular Biology
Yan Huang, Chenxu Zhao, Yezi Kong, Panpan Tan, Siqi Liu, Yaoquan Liu, Fangyuan Zeng, Yang Yuan, Baoyu Zhao, Jianguo Wang
Summary: In dairy cows during the transition period, the NEFA-induced PERK-eIF2 alpha signaling pathway plays a crucial role in promoting lipid synthesis and oxidation while inhibiting the assembly and secretion of VLDL. Activation of this pathway may help alleviate NEB by counteracting acid-induced lipotoxicity and providing energy.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Brenda Landvoigt Schmitt, Bruna Ferreira Leal, Mariana Leyser, Muriel Primon de Barros, Danielle Silva Trentin, Carlos Alexandre Sanchez Ferreira, Silvia Dias de Oliveira
Summary: The mechanism of antimicrobial resistance and persistence ability of Acinetobacter baumannii may be associated with the outer membrane proteins OmpW and OmpA, highlighting them as potential targets for drug development against A. baumannii persisters.
Article
Plant Sciences
Guanfeng Wang, Geling Gao, Xiangna Yang, Xiangdong Yang, Pengda Ma
Summary: Casein kinase CK2, a highly conserved serine/threonine protein kinase, plays important roles in biological processes of plants with complex structural features and diverse physiological functions. CK2 in plants is encoded by multiple genes, existing in monomeric and oligomeric forms. Regulation of CK2 activity is crucial for subsequent research on plants.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Microbiology
Daniel Scheller, Christian Twittenhoff, Franziska Becker, Marcel Holler, Franz Narberhaus
Summary: The study found evidence for a temperature-modulated RNA structure in the 5'-untranslated region of the OmpA transcript, supporting the hypothesis that melting of the RNA structure relieves translational repression. This allows for increased translation of the transcript at higher temperatures, with biochemical experiments showing that melting of the RNA structure permits ribosome binding to the 5'-UTR.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jingxuan Shi, Heng Chi, Aiping Cao, Yinna Song, Min Zhu, Lilin Zhang, Fuzhou Xu, Jinhai Huang
Summary: The study developed a method of using anti-OmpF antibody-immunomagnetic beads combined with PCR detection to effectively capture and detect Yersinia enterocolitica in food samples. This method showed higher capture efficiency and detection limit compared to traditional culture methods, providing a rapid and accurate alternative for Y. enterocolitica detection in food.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Immunology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, David Perez-Pascual, Rebecca J. Stevick, Clayton Birkett, Jean-Marc Ghigo, Mark J. McBride
Summary: Flavobacterium columnare, a costly pathogen in the freshwater fish-farming industry, produces siderophores for iron uptake. This study identifies genes involved in iron uptake and reveals their importance for growth under iron-limited conditions. Mutants with deletions in these genes showed decreased virulence but still caused infection. Survivors of exposure to these mutants were partially protected against later infection by the wild-type pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Ecology
Ming-Xuan Tang, Tong-Tong Pei, Qi Xiang, Zeng-Hang Wang, Han Luo, Xing-Yu Wang, Yang Fu, Tao Dong
Summary: Vibrio cholerae uses its type VI secretion system to survive in competitive communities, and the effectiveness of this system is influenced by environmental cations and temperature.