Article
Biochemistry & Molecular Biology
S. E. Faraj, W. M. Valsecchi, N. T. Cerf, N. U. Fedosova, R. C. Rossi, M. R. Montes
Summary: The study found that although Na+ competes with H+ in ATPase activity, it was not possible to isolate a Na+-occluded state nor reveal any Na+ influx related to H,K-ATPase activity. The high rate of the E2K -> E1 transition in H,K-ATPase, not compatible with the presence of a K+-occluded form, agrees with the negligible level of occluded Rb+ found in the absence of added ligands.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Multidisciplinary Sciences
Victoria C. Young, Pablo Artigas
Summary: Utilizing state-specific engineered-disulfide cross-linking, it was demonstrated that the transmembrane segment 2 (M2) of the Na+/K+ pump moves in directions inconsistent with distances observed in existing crystal structures of the pump. This movement was characterized using voltage-clamp fluorometry in single-cysteine mutants, revealing state-dependent fluorescence changes upon labeling with tetramethylrhodamine-6-maleimide (TMRM).
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ryuta Kanai, Flemming Cornelius, Bente Vilsen, Chikashi Toyoshima
Summary: In this study, the 3.3 angstrom resolution structure of Na+,K+-ATPase (NKA) in the E2·2K(+) state was resolved using cryo-electron microscopy. The transition from the E2P state to the E2·2K(+) state involves the closure and locking of the extracellular ion pathway, as well as events at the phosphorylation site over 50 angstrom away. Furthermore, the binding of ATP to NKA in the E2·2K(+) state was found to relax the gating machinery and greatly accelerate the release of K+ into the cytoplasm.
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Lucy Kate Ladefoged, Birgit Schiott, Natalya U. Fedosova
Summary: The study reveals significant differences in the binding properties of different subfamilies of cardiotonic steroids with the Na+,K+-ATPase. While bufadienolides show beneficial effects with K+, they differ from cardenolides which have an antagonistic relationship with K+. Through simulations, it was found that the binding of bufadienolides is influenced by amino acid coordination patterns and the presence of cations, illustrating the complex interplay of factors in these interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Multidisciplinary Sciences
Lisa Z. Fang, Victoria Linehan, Maria Licursi, Christian O. Alberto, Jacob L. Power, Matthew P. Parsons, Michiru Hirasawa
Summary: Hypothalamic inflammation can exacerbate obesity and fatty liver by stimulating appetite-promoting neurons.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Victoria C. Young, Hanayo Nakanishi, Dylan J. Meyer, Tomohiro Nishizawa, Atsunori Oshima, Pablo Artigas, Kazuhiro Abe
Summary: This study investigates ion transport mechanisms by introducing mutations to convert an electroneutral proton/potassium pump into a prototypical electrogenic sodium/potassium pump, explaining their selectivity and phosphorylation mechanisms.
NATURE COMMUNICATIONS
(2022)
Article
Fisheries
Ahmed Badr, Jaakko Haverinen, Matti Vornanen
Summary: There are significant differences in Na,K-ATPase activity among different tissues in roach. The number of Na,K-ATPase alpha-subunits is strongly correlated with the activity. Cold acclimation increases the number of alpha-subunits in the heart, kidney and brain, but does not increase Na,K-ATPase activity. In the gills, the alpha-subunits do not change during cold acclimation, but Na,K-ATPase activity is depressed. Tissue-specific differences in Na,K-ATPase activity are not only determined by the density of alpha-subunits, but also affected by other factors.
Article
Multidisciplinary Sciences
Tomas Heger, Marek Zatloukal, Martin Kubala, Miroslav Strnad, Jiri Gruz
Summary: A newly discovered trimeric procyanidin C1, found in Viola odorata root extract, was shown to strongly inhibit Na+,K+-ATPase activity. Leaf and seed extracts of the plant showed no significant inhibitory effects on the enzyme. Through compound screening and bioactivity assessment, the active components of V. odorata root extract were identified.
SCIENTIFIC REPORTS
(2022)