Article
Plant Sciences
Chase Beathard, Sutton Mooney, Raed Al-Saharin, Aymeric Goyer, Hanjo Hellmann
Summary: Plants rely on the ubiquitin proteasome pathway to rapidly respond to environmental changes and abiotic stress. The study reveals that MYB1, MYB25, and MYB109, members of the R2R3 S23 MYB transcription factor subfamily, are degraded by the 26S proteasome, with elevated expression of MYB25 reducing sensitivities to stress in Arabidopsis. Overexpression of MYB25 affects cellular stress pathways related to biotic and abiotic stress control in seedlings.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Biochemistry & Molecular Biology
Charlotte Nugues, Dayani Rajamanoharan, Robert D. Burgoyne, Lee P. Haynes, Nordine Helassa
Summary: Lysosomes play a crucial role in mitosis through dynamic redistribution and exocytosis. Inhibiting lysosomal exocytosis results in mitosis failure.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ping Huang, Fei-Jun Luo, You-Chu Ma, Si-Xian Wang, Jia Huang, Dan-Dan Qin, Fei-Fei Xue, Bi-Yang Liu, Qiang Wu, Xiao-Ling Wang, Gao-Qiang Liu
Summary: A novel antioxidant peptide (GLP4) was identified from Ganoderma lingzhi mycelium, which exhibited both direct and indirect antioxidant activities. GLP4 could enter human umbilical vein endothelial cells (HUVECs) as an exogenous substance and activate the Nrf2/antioxidant response element (ARE) signaling pathway, providing cytoprotective effects against hydrogen peroxide (H2O2)-induced HUVECs.
Article
Biochemistry & Molecular Biology
Christine R. Langlois, Viola Beier, Ozge Karayel, Jakub Chrustowicz, Dawafuti Sherpa, Matthias Mann, Brenda A. Schulman
Summary: Cells remodel their proteomes to adjust to environmental metabolism demands. The GID E3 ligase regulates the transition from gluconeogenesis to glycolysis, while the Gid10 substrate receptor plays a role in responding to changes in temperature, osmolarity, and nutrient availability, regulating plasma membrane quality control.
Article
Plant Sciences
Mengqi Ding, Yuqi He, Kaixuan Zhang, Jinbo Li, Yaliang Shi, Mengyu Zhao, Yu Meng, Milen I. Georgiev, Meiliang Zhou
Summary: This study reveals the mechanism of a transcription suppressor, FtERF-EAR3, in regulating flavonoid biosynthesis in Tartary buckwheat. FtERF-EAR3 inhibits FtF3H expression and flavonoid biosynthesis by binding to the GCC-box in the promoter of FtF3H. The gene expression of FtERF-EAR3 is induced by JA and the protein is degraded through the FtBPM3-dependent 26S proteasome pathway. Understanding the regulation mechanism of flavonoid biosynthesis in Tartary buckwheat is significant for improving its production efficiency.
Article
Cell Biology
Julia Katharina Charlotte Kress, Christina Jessen, Anita Hufnagel, Werner Schmitz, Thamara Nishida Xavier da Silva, Ancely Ferreira dos Santos, Laura Mosteo, Colin R. Goding, Jose Pedro Friedmann Angeli, Svenja Meierjohann
Summary: The redox regulator NRF2 is activated during oxidative and electrophilic stress and is involved in redox regulation, metabolism, tumor therapy resistance, and immune suppression. This study reveals a previously unrecognized link between the integrated stress response (ISR) and NRF2, mediated by the ISR effector ATF4. NRF2 supports ATF4-induced cells by increasing cystine uptake and upregulating thioredoxin-related genes. These findings highlight the importance of the NRF2 response in cellular resilience.
Article
Genetics & Heredity
Tammy Lu, Ryan B. Smit, Hanifa Soueid, Paul E. Mains
Summary: Microtubule severing plays a crucial role in cell structure and division. The protein katanin, composed of MEI-1/MEI-2 subunits, is required for oocyte meiotic spindle formation but must be inactivated for mitosis to proceed. Katanin activity is regulated by two ubiquitin-based protein degradation pathways, as well as by the ubiquitin ligase HECD-1. In C. elegans, components of the striatin-interacting kinase phosphatase complex also affect katanin function, with some acting as inhibitors in mitosis and activators in meiosis. These findings suggest that different subunits of the complex have diverse functions in regulating cell proliferation and signaling pathways.
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Article
Plant Sciences
Maria Cecilia Terrile, Nuria Malena Tebez, Silvana Lorena Colman, Julieta Lisa Mateos, Esperanza Morato-Lopez, Nuria Sanchez-Lopez, Alicia Izquierdo-Alvarez, Anabel Marina, Luz Irina A. Calderon Villalobos, Mark Estelle, Antonio Martinez-Ruiz, Diego Fernando Fiol, Claudia Anahi Casalongue, Maria Jose Iglesias
Summary: E3 ubiquitin ligases are crucial in plant biology and play a key role in the perception and signaling of hormones. The redox modification of proteins through S-nitrosation serves as an important regulatory mechanism in hormonal signaling pathways. This study confirmed the S-nitrosation of TIR1 and COI1, highlighting the functional relevance of this modification in the response to auxins and jasmonates.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sharadha Dayalan Naidu, Plamena R. Angelova, Elena V. Knatko, Chiara Leonardi, Miroslav Novak, Laureano de la Vega, Ian G. Ganley, Andrey Y. Abramov, Albena T. Dinkova-Kostova
Summary: Transcription factor Nrf2 plays a crucial role in maintaining cellular redox homeostasis and mitochondrial health in lung cancer cells with loss-of-function Keap1 mutations.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Plant Sciences
Huimin Sun, Jiatao Li, Xu Li, Qian Lv, Liuping Chen, Bingxin Wang, Liqun Li
Summary: TaSADR1 gene was found to negatively regulate drought stress response in wheat and achieved this function by regulating the expression of stress-related genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Han Wang, Yingjie Zhang, Xiaofeng Feng, Jiayi Hong, Muhammad Aamir Manzoor, Xinyue Zhou, Qifang Zhou, Yongping Cai
Summary: This study revealed that a transcription factor, PbMYB80, inhibits stone cell development in pear fruit by inhibiting the expression of lignin biosynthesis genes. Additionally, PbRHY1 protein acts as an E3 ubiquitin ligase to degrade PbMYB80 through ubiquitination. These findings contribute to a better understanding of the regulation of lignin biosynthesis in pear fruit.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Amanda B. Chai, Richard Callaghan, Ingrid C. Gelissen
Summary: The study demonstrates that NEDD4-1 regulates the expression and activity of P-gp, thereby facilitating the clearance of Aβ peptides from the brain. This finding offers a potential approach for the treatment of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Guangqiang Zhang, Junjiao Yang, Meng Zhang, Qinxue Li, Yunzhen Wu, Xiaoyu Zhao, Huifei Zhang, Yong Wang, Jiajie Wu, Wei Wang
Summary: The study revealed that TaPUB1 enhances the resistance of wheat to Cd stress by modulating the stability of TaIRT1 and TaIAA17 proteins in three ways. TaPUB1 overexpression lines showed reduced Cd accumulation and ROS accumulation, with inhibitory effects on Cd uptake.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)