Article
Multidisciplinary Sciences
Lei Wan, Xi Chen, Wolfhard Almers
Summary: Syntaxin plays a crucial role in catalyzing membrane fusion during exocytosis and forms clusters in the plasma membrane. The mechanism of cluster formation differs between on-granule and off-granule clusters in live PC12 cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Rajkishor Nishad, Miguel Betancourt-Solis, Himani Dey, Ruth Heidelberger, James A. McNew
Summary: The remarkable signaling abilities of retinal neurons are regulated by syntaxin3B, as well as other presynaptic proteins and complexins.
Article
Biochemistry & Molecular Biology
Annemiek A. van Berkel, Tatiana C. Santos, Hesho Shaweis, Jan R. T. van Weering, Ruud F. Toonen, Matthijs Verhage
Summary: Loss of the exocytic protein MUNC18-1 or its target-SNARE partners SNAP25 and syntaxin-1 leads to rapid, cell-autonomous neurodegeneration, with abnormalities observed in cis-, medial-, and trans-Golgi compartments. While anterograde transport pathways remain intact, deficiencies in retrograde transport to the Golgi may explain the observed cellular abnormalities and cell death in MUNC18-1-deficient neurons.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Daniel C. Puntman, Swati Arora, Margherita Farina, Ruud F. Toonen, Matthijs Verhage
Summary: Neuropeptide secretion from dense-core vesicles (DCVs) in the brain is controlled by MUNC18-1, which is essential for maintaining proper synaptic transmission and neurodevelopmental processes. Impairment in neuropeptide secretion due to reduced MUNC18-1 expression may contribute to the symptoms observed in certain neurological disorders.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Femke M. Feringa, Annemiek A. van Berkel, Anushka Nair, Matthijs Verhage
Summary: Depletion of presynaptic proteins MUNC18-1 or syntaxin-1 triggers an atypical, staged cell death pathway characterized by consecutive neurite retraction, ultimately leading to, but not driven by, apoptosis.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biology
Xianping Wang, Jihong Gong, Le Zhu, Huidan Chen, Ziqi Jin, Xiaoqiang Mo, Shen Wang, Xiaofei Yang, Cong Ma
Summary: This study reveals that the extension of the 3a domain in Munc18-1/Syntaxin-1 is crucial for the Munc13-1 mediated SNARE complex assembly and membrane fusion. Specific amino acid residues play a key role in this process.
Article
Plant Sciences
Steven Beuder, Cecilia Lara-Mondragon, Alexandria Dorchak, Cora A. MacAlister
Summary: The study demonstrated that mutant alleles of the SEC1A protein act as suppressors of growth defects in pollen tubes. These suppressors improve seed set, reduce glycoprotein secretion, and alleviate PT growth defects. The absence of SEC1A leads to reduced pollen germination and tube elongation, with the protein playing a crucial role in promoting pollen fertility.
Article
Multidisciplinary Sciences
Karolina P. Stepien, Josep Rizo
Summary: The study demonstrates that decreasing the synaptobrevin-to-lipid ratio to very low levels leads to inefficient fusion, but synaptotagmin-1 strongly stimulates fusion under these conditions. Anchoring SNAP-25 on the syntaxin-1 liposomes dramatically enhances fusion efficiency. Additionally, there is a synergy between synaptotagmin-1 and membrane anchoring of SNAP-25, allowing efficient fusion between liposomes with low synaptobrevin densities and low syntaxin-1 densities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Karolina P. Stepien, Junjie Xu, Xuewu Zhang, Xiaochen Bai, Josep Rizo
Summary: This study reveals the binding mechanism of Munc18-1 to cross-linked syntaxin-1 and synaptobrevin through cryo-EM structures, and explores the mechanism of SNARE complex assembly. The results demonstrate that these interactions are critical for initiating SNARE complex assembly.
Article
Neurosciences
Jihong Gong, Xianping Wang, Chaoyang Cui, Yuyang Qin, Ziqi Jin, Cong Ma, Xiaofei Yang
Summary: Experimental data support the notion that opening of the syntaxin-1 linker region is a key step in initiating SNARE complex assembly, while the extended state of Munc18-1 domain 3a can compensate for this action.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Junhong Wu, Hui Zhang, Liu Yang, Yuanyuan Chen, Jiyuan Li, Min Yang, Xiaogang Zhang, Changlong He, Xuefeng Wang, Xin Xu
Summary: This study investigates the role of STX7 in epilepsy and finds that reduced expression of STX7 in the epileptic brain is associated with increased susceptibility to epileptic seizures. Overexpression of STX7, on the other hand, decreases susceptibility to seizures and alleviates epileptic activity. It is also found that STX7 affects the release of inhibitory neurotransmitters, but not the intrinsic excitability of neurons.
NEUROBIOLOGY OF DISEASE
(2023)
Review
Oncology
Fajuan Tang, Dongqiong Xiao, Lin Chen, Hu Gao, Xihong Li
Summary: Munc18-1 plays a crucial role in regulating synaptic vesicle fusion and neurotransmitter release, as well as in neurosynaptic plasticity, neurodevelopment, and neuroendocrine cell release functions. Aberrant expression of Munc18-1 is implicated in the pathogenesis of various neurological diseases.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Plant Sciences
Martiniano Maria Ricardi, Niklas Wallmeroth, Cecilia Cermesoni, Dietmar Gerald Mehlhorn, Sandra Richter, Lei Zhang, Josephine Mittendorf, Ingeborg Godehardt, Kenneth Wayne Berendzen, Edda von Roepenack-Lahaye, York-Dieter Stierhof, Volker Lipka, Gerd Juergens, Christopher Grefen
Summary: The final step in secretion is membrane fusion facilitated by SNARE proteins that reside in opposite membranes. The formation of a trans-SNARE complex between one R and three Q coiled-coiled SNARE domains drives the final approach of the membranes providing the mechanical energy for fusion. Biological control of this mechanism is exerted by additional domains within some SNAREs.
Review
Cell Biology
I. -Ying Kuo, Chih-Hsiung Hsieh, Wan-Ting Kuo, Chih-Peng Chang, Yi-Ching Wang
Summary: Cells in the tumor microenvironment require a class of checkpoints to regulate various secretion and transport processes. Recent studies have shown that Rab proteins mediate communication between diverse cargoes and substrates through vesicular trafficking and secretion pathways in the tumor microenvironment. This review focuses on the role and regulation of vesicular secretion pathways in the tumor microenvironment, and describes the latest research advances and future directions.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tingting Fan, Yuemin Fan, Yang Yang, Dong Qian, Yue Niu, Lizhe An, Yun Xiang
Summary: The study found that SEC1A, a member of the Sec1/Munc18 (SM) protein family in Arabidopsis thaliana, plays a crucial role in polar growth of pollen tubes. SEC1A interacts with the exocyst subunit SEC6, and they cooperatively affect the secretion rate and distribution at the tip of the pollen tube. Loss of function of SEC1A and SEC6 leads to the disorder of pollen tube cell wall components.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Engineering, Electrical & Electronic
Istvan Gyongy, Neil Calder, Amy Davies, Neale A. W. Dutton, Rory R. Duncan, Colin Rickman, Paul Dalgarno, Robert K. Henderson
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2018)
Article
Optics
Istvan Gyongy, Amy Davies, Benjamin Gallinet, Neale A. W. Dutton, Rory R. Duncan, Colin Rickman, Robert K. Henderson, Paul A. Dalgarno
Article
Biochemistry & Molecular Biology
Christopher Allan, Miroslav Kosar, Christina V. Burr, C. Logan Mackay, Rory R. Duncan, Alison N. Hulme
Article
Biochemistry & Molecular Biology
Nisha Kriplani, Rory R. Duncan, Nicholas R. Leslie
Article
Biochemistry & Molecular Biology
Rebecca S. Saleeb, Deirdre M. Kavanagh, Alison R. Dun, Paul A. Dalgarno, Rory R. Duncan
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Tushar R. Choudhary, Michael G. Tanner, Alicia Megia-Fernandez, Kerrianne Harrington, Harry A. Wood, Adam Marshall, Patricia Zhu, Sunay Chankeshwara, Debaditya Choudhury, Graham Monro, Muhammed Ucuncu, Fei Yu, Rory R. Duncan, Robert R. Thomson, Kevin Dhaliwal, Mark Bradley
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Ian T. Lobb, Pierre Morin, Kirsty Martin, Hazel C. Thoms, Jimi C. Wills, Xhordi Lleshi, Karl C. F. Olsen, Rory R. Duncan, Lesley A. Stark
Summary: This study reveals the mechanism of elevated NF-kappa B activity in cancer, highlighting the accumulation of the autophagy receptor SQSTM1/p62 in nucleolar aggresomes and its transport role for proteins like RelA.
MOLECULAR CANCER RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Jessica Valli, Adrian Garcia-Burgos, Liam M. Rooney, Beatriz Vale de Melo E. Oliveira, Rory R. Duncan, Colin Rickman
Summary: Choosing the right super-resolution technique is crucial for understanding biological relevance. Due to inevitable trade-offs in different imaging capabilities, many researchers find it challenging to select the most suitable technique for their study.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Proceedings Paper
Engineering, Biomedical
Istvan Gyongy, Andrew Green, Sam W. Hutchings, Amy Davies, Neale A. W. Dutton, Rory R. Duncan, Colin Rickman, Robert K. Henderson, Paul A. Dalgarno
HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY IV
(2019)
Proceedings Paper
Optics
Istvan Gyongy, Amy Davies, Allende Miguelez Crespo, Andrew Green, Neale A. W. Dutton, Rory R. Duncan, Colin Rickman, Robert K. Henderson, Paul A. Dalgarno
HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY III: TOWARD BIG DATA INSTRUMENTATION AND MANAGEMENT
(2018)
Article
Biochemistry & Molecular Biology
Katharina N. Richter, Natalia H. Revelo, Katharina J. Seitz, Martin S. Helm, Deblina Sarkar, Rebecca S. Saleeb, Elisa D'Este, Jessica Eberle, Eva Wagner, Christian Vogl, Diana F. Lazaro, Frank Richter, Javier Coy-Vergara, Giovanna Coceano, Edward S. Boyden, Rory R. Duncan, Stefan W. Hell, Marcel A. Lauterbach, Stephan E. Lehnart, Tobias Moser, Tiago F. Outeiro, Peter Rehling, Blanche Schwappach, Ilaria Testa, Bolek Zapiec, Silvio O. Rizzoli