Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Food Science & Technology
Wenqi Shi, Jie Li, Yanfang Chen, Yefu Chen, Xuewu Guo, Dongguang Xiao
Summary: Metabolic engineering strategies were utilized to enhance the production of C6-C10 fatty acid ethyl esters in Saccharomyces cerevisiae, including improving precursor accumulation, increasing alcohol acyltransferase activity, and facilitating ester synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Oncology
Zhihao Jia, Xiyue Chen, Jingjuan Chen, Lijia Zhang, Stephanie N. Oprescu, Nanjian Luo, Yan Xiong, Feng Yue, Shihuan Kuang
Summary: This study identifies ACSS3 as the key enzyme for propionate metabolism and demonstrates that accumulation of propionate promotes obesity and Type 2 diabetes through triggering adipocyte autophagy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sayoko Kuroha, Yusaku Katada, Yosuke Isobe, Haruki Uchino, Kyosuke Shishikura, Takashi Nirasawa, Kazuo Tsubota, Kazuno Negishi, Toshihide Kurihara, Makoto Arita
Summary: Docosahexaenoic acid (DHA) and ultra-long-chain polyunsaturated fatty acids (ULC-PUFAs) are enriched in retinal photoreceptor membrane phospholipids. The enzyme ACSL6 plays a crucial role in the synthesis and enrichment of these lipids. Genetic deletion of ACSL6 leads to depletion of di-DHA and ULC-PUFA-containing phospholipids, resulting in visual impairment and progressive decrease of retinal thickness.
Article
Biochemistry & Molecular Biology
Veronika Mater, Sabine Eisner, Cornelia Seidel, Dirk Schneider
Summary: Tuberculosis, a common infectious disease, infects about one-fourth of the global population. The causative agent, Mycobacterium tuberculosis, can enter a dormant state and display antibiotic resistance. The study investigates the membrane association and substrate specificity of MbFACL6, a potential target for treating resistant strains. MbFACL6 is found to be a peripheral membrane-attached protein and shows a broad spectrum of substrate tolerance.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Immunology
Abbe N. Vallejo, Henry J. Mroczkowski, Joshua J. Michel, Michael Woolford, Harry C. Blair, Patricia Griffin, Elizabeth McCracken, Stephanie J. Mihalik, Miguel Reyes-Mugica, Jerry Vockley
Summary: The study found that patients with VLCADD exhibit systemic inflammatory responses during symptomatic and asymptomatic periods, with an abundance of immune cells expressing high levels of inflammatory mediators. Patients with recurrent hospitalisations showed sustained high levels of plasma cytokines and higher intracellular stores of inflammatory molecules in monocytes.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yongjie Ma, Junyi Zha, XiangKun Yang, Qianjin Li, Qingfu Zhang, Amelia Yin, Zanna Beharry, Hanwen Huang, Jiaoti Huang, Michael Bartlett, Kaixiong Ye, Hang Yin, Houjian Cai
Summary: The study revealed that ACSL1 is highly expressed in prostate tumors, promoting the generation of fatty acyl-CoAs and facilitating cancer cell growth. ACSL1 modulates mitochondrial respiration, beta-oxidation, and ATP production, impacting fatty acid metabolism and prostate cancer progression.
Article
Biochemistry & Molecular Biology
Qiaoyun Chu, Ping Liu, Yihan Song, Ronghui Yang, Jing An, Xuewei Zhai, Jing Niu, Chuanzhen Yang, Binghui Li
Summary: Reprogramming of lipid metabolism is a hallmark of cancer, but the involvement of specific fatty acids and enzymes in tumorigenesis is not well understood. This study shows that depletion of the enzyme acetyl-CoA carboxylase inhibits the synthesis and elongation of very long-chain fatty acids in human cancer cells. Deficiency of very long-chain fatty acids impairs mitochondrial morphology and makes cancer cells susceptible to oxidative stress and cell death.
Article
Oncology
Daniel L. Pouliquen, Giacomo Ortone, Letizia Rumiano, Alice Boissard, Cecile Henry, Stephanie Blandin, Catherine Guette, Chiara Riganti, Joanna Kopecka
Summary: Cross-species investigations of cancer invasiveness have identified new biomarkers for improving tumor diagnosis and prognosis. This study analyzed rat tumors and human cell lines to identify common features associated with mitochondrial proteome rewiring. The differential expression of mitochondrial proteins, particularly ACADL, was found to be associated with invasiveness in mesothelioma.
Article
Medical Laboratory Technology
Salim Ahmed Al-Busaidi, Jawaher Al Al Nou'mani, Zubaida Al-Falahi, Rajaa Al-Farsi, Suneel Kumar, Fathiya Al-Murshedi, Kathiya Awlad-Thani, Ayda Al Nabhani, Abdullah M. Al Alawi
Summary: A 14-year-old boy diagnosed with very long-chain acyl-CoA dehydrogenase deficiency (VLCADD) also developed type 1 diabetes mellitus (DM1). Managing this patient is challenging due to potentially paradoxical life-threatening complications of the two diseases.
CLINICAL BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Masato Ikeda, Keisuke Takahashi, Tatsunori Ohtake, Ryosuke Imoto, Haruka Kawakami, Mikiro Hayashi, Seiki Takeno
Summary: Recent research found a unique mechanism of lipid homeostasis in Corynebacterium glutamicum, involving a futile cycle of acyl-CoA hydrolysis and resynthesis mediated by acyl-CoA thioesterase (Tes) and acyl-CoA synthetase (FadD). Engineering this cycle in a high-fatty-acid producer significantly increases production, offering a useful strategy for improving fatty acid production in this industrially important microorganism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Elena Heng, Yi Wee Lim, Chung Yan Leong, Veronica W. P. Ng, Siew Bee Ng, Yee Hwee Lim, Fong Tian Wong
Summary: By engineering methods, the production of armenciaspirol, a compound with antibacterial activity, can be improved by seven to forty nine times. This can be achieved through the removal of competing polyketide clusters, perturbation of genes in metabolic pathways, and the expression of heterologous fatty acyl-CoA synthase. These methods not only increase the production yields but also lead to the discovery of new intermediates and analogs of armenciaspirol.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemical Research Methods
Karolis Petkevicius, Eleni Koutsoumpeli, Petri Christina Betsi, Bao-Jian Ding, Kanchana Rueksomtawin Kildegaard, Hilbert Jensen, Nora Mezo, Andrea Mazziotta, Anders Gabrielsson, Christina Sinkwitz, Bettina Lorantfy, Carina Holkenbrink, Christer Lofstedt, Dimitris Raptopoulos, Maria Konstantopoulou, Irina Borodina
Summary: This study developed a biotechnological method to produce the sex pheromone of the European corn borer using engineered yeast, achieving high titers of the pheromone component. The research opens up possibilities for fermentation-based production of ECB pheromones for pest management.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Rasmus Stenlid, Hannes Manell, Rikard Seth, Sara Y. Cerenius, Azazul Chowdhury, Camilla Roa Cortes, Isabelle Nyqvist, Thomas Lundqvist, Maria Halldin, Peter Bergsten
Summary: This study found that patients with VLCAD and MCAD have significantly lower plasma glucagon concentrations compared to patients with CUD, while the concentrations in the CUD group were similar to normative data, suggesting that glucagon secretion may be impaired in patients with VLCAD or MCAD.
Review
Biochemistry & Molecular Biology
Sylwia Szrok-Jurga, Aleksandra Czumaj, Jacek Turyn, Areta Hebanowska, Julian Swierczynski, Tomasz Sledzinski, Ewa Stelmanska
Summary: Fatty acid metabolism, especially beta-oxidation, plays a crucial role in human physiology and pathology. Beta-oxidation is not only involved in energy metabolism but also contributes to the synthesis of various important compounds. This review provides an overview of the importance of mitochondrial and peroxisomal beta-oxidation in different organs and cells and discusses the diseases associated with fatty acid oxidation disturbances. The review also highlights the abnormalities of fatty acid oxidation in cancer cells and diseases caused by mutations in enzymes involved in fatty acid oxidation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Tegan M. Haslam, Ivo Feussner
Summary: This review discusses the diversity of sphingolipid profiles in different plant lineages and how they contribute to metabolic diversity and physiological functions. Sphingolipids are essential for various cellular processes in plants and their content varies across different species. The review focuses on model systems such as Arabidopsis thaliana, Oryza sativa, and Physcomitrium patens, summarizing the known sphingolipid content and discussing modifications to hydrophobic ceramides and polar headgroups. This knowledge serves as a framework for understanding sphingolipid metabolism in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Gloria O. Okooboh, Ilka Haferkamp, Marzieh Valifard, Benjamin Pommerrenig, Amelie Kelly, Ivo Feussner, Horst Ekkehard Neuhaus
Summary: Overexpression of vacuolar sugar transporter TST1 can lead to higher seed lipid levels and total seed yield in Arabidopsis. By constitutively overexpressing TST1 from sugar beet in the crop species Camelina sativa, researchers have observed similar seed characteristics and improved root architecture, resulting in optimized seed properties and yield under limited water supply. This finding suggests the potential to create crops with enhanced drought tolerance and increased yield through increased vacuolar TST activity.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Stefanie Koenig, Jasmin Goemann, Agnieszka Zienkiewicz, Krzysztof Zienkiewicz, Dorothea Meldau, Cornelia Herrfurth, Ivo Feussner
Summary: Ceramides and long-chain bases are sphingolipids involved in plant programmed cell death. Accumulation of long-chain bases may result in cell death, and salicylic acid and EDS1 play important roles in this process.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Microbiology
Yulei Zhao, Nisha Agrawal, Hassan Ghareeb, Mohammad Tanbir Habib, Sascha Dickmeis, Jens Schwachtje, Tim E. Iven, Joachim Kopka, Ivo Feussner, Jan Schirawski
Summary: Genome comparison between the maize pathogens Ustilago maydis and Sporisorium reilianum identified a diversity region containing nearly 30 effector gene candidates. Further investigation revealed the high expression of a virulence-associated gene, vag2, and its interaction with the maize enzyme ZmCM2. The study suggests that Vag2 lowers plant SA-induced defenses by inhibiting the reaction catalyzed by ZmCM2, thereby contributing to fungal virulence.
Article
Chemistry, Physical
Katharina Vollheyde, Karin Kuehnel, Felix Lambrecht, Steffen Kawelke, Cornelia Herrfurth, Ivo Feussner
Summary: Wax esters (WE) are neutral lipids formed by the transesterification of activated fatty acyl moiety to a fatty alcohol. Producing WE in bacteria and plants through genetic engineering is an important research field for improving sustainability and reducing costs. However, detailed understanding of the catalytic mechanism and structural determinants is still lacking.
Article
Plant Sciences
Lennart Mohnike, Weijie Huang, Brigitte Worbs, Kirstin Feussner, Yuelin Zhang, Ivo Feussner
Summary: This study identified three novel metabolites of N-hydroxy pipecolic acid (NHP) in Arabidopsis thaliana leaves and investigated their formation mechanism and functions. MeNHP was found to be a storage or transport form of NHP, while NHP-OGlc-Hex and NHP-OGlc-derivative may have other physiological functions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Philipp William Niemeyer, Iker Irisarri, Patricia Scholz, Kerstin Schmitt, Oliver Valerius, Gerhard H. Braus, Cornelia Herrfurth, Ivo Feussner, Shrikant Sharma, Anders S. Carlsson, Jan de Vries, Per Hofvander, Till Ischebeck
Summary: There are plant organs and developmental stages that can withstand severe water loss. The tubers of yellow nutsedge have seed-like characteristics and show upregulation of seed proteins. In contrast, the tubers of purple nutsedge do not accumulate oil and lack desiccation-tolerance. The expression of specific transcription factors may contribute to the differences between the two nutsedge species.
Article
Biochemistry & Molecular Biology
Gabriel Deslandes-Herold, Martina Zanella, Erik Solhaug, Michaela Fischer-Stettler, Mayank Sharma, Leo Buergy, Cornelia Herrfurth, Maite Colinas, Ivo Feussner, Melanie R. Abt, Samuel C. Zeeman
Summary: The PRK/Rubisco shunt enhances the carbon efficiency of storage lipid biosynthesis in green Brassicaceae seeds. This pathway utilizes metabolites generated by the nonoxidative steps of the pentose phosphate pathway to recycle CO2 molecules released during fatty acid synthesis. Loss of PRK affects seed glycolysis, starch metabolism, and photosynthesis.
Article
Plant Sciences
Yi-Tse Liu, Jennifer Senkler, Cornelia Herrfurth, Hans-Peter Braun, Ivo Feussner
Summary: Mitochondria are critical for various biological processes and require a defined membrane lipid composition for their structural and functional integrity. While some lipids are self-synthesized, others are transferred from the endoplasmic reticulum to mitochondria. However, the knowledge on lipid biosynthesis in mitochondria and the mechanisms of maintaining lipid homeostasis is limited. This study characterizes the lipidome and proteins involved in lipid metabolism in plant mitochondria, providing a foundation for further understanding the functional roles of lipids in biochemical reactions.
Article
Plant Sciences
M. Lewandowska, A. Zienkiewicz, K. Feussner, S. Koenig, L. Kunst, I. Feussner
Summary: TAG accumulation upon wounding is not dependent on JA-Ile or ABA. The newly synthesized TAG species are composed of unsaturated fatty acids of membrane origin and likely serves as a transient energy store.
Article
Plant Sciences
Milena Lewandowska, Krzysztof Zienkiewicz, Agnieszka Zienkiewicz, Amelie Kelly, Stefanie Koenig, Kirstin Feussner, Ljerka Kunst, Ivo Feussner
Summary: This study investigates the role of wax biosynthesis and respective genes in response to plant wounding. The results show that genes coding for enzymes of surface wax synthesis are induced upon wounding in an ABA-dependent manner. The ABA-dependent transcription factor MYB96 is identified as a key regulator of wax biosynthesis. Furthermore, the study reveals that high humidity prevents wound-induced wax accumulation in Arabidopsis thaliana leaves.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Magdalena Miklaszewska, Krzysztof Zienkiewicz, Ewa Klugier-Borowska, Marcin Rygielski, Ivo Feussner, Agnieszka Zienkiewicz
Summary: This study provides new insights into the microautophagy-mediated degradation mechanism of lipid droplets (LDs) during Arabidopsis seed germination. The researchers discovered how LDs enter the central vacuole and undergo degradation there, as well as the interaction between LD proteins and AUTOPHAGY-RELATED PROTEIN 8. These findings reveal the mechanism of LD degradation during seed germination and highlight the role of structural LD proteins in this process.
Article
Plant Sciences
Athanas Guzha, Robert McGee, Patricia Scholz, Denise Hartken, Daniel Ludke, Kornelia Bauer, Marion Wenig, Krzysztof Zienkiewicz, Cornelia Herrfurth, Ivo Feussner, A. Corina Vlot, Marcel Wiermer, George Haughn, Till Ischebeck
Summary: Plant cell walls serve as barriers against microbial pathogens, and BXL4 plays a crucial role in plant immunity by removing side-chains of polysaccharides in the cell wall.
Article
Biochemistry & Molecular Biology
Indira Saado, Khong-Sam Chia, Ruben Betz, Andre Alcantara, Aladar Pettko-Szandtner, Fernando Navarrete, John C. D'Auria, Michael Kolomiets, Michael Melzer, Ivo Feussner, Armin Djamei
Summary: The study shows that the smut fungus Ustilago maydis secretes effector proteins to suppress host defense responses in maize plants, with Rip1 playing a key role in inhibiting host immunity and affecting antioxidant reactions. By relocalizing the host factor ZmLox3, the fungus can partially suppress ROS burst responses in maize plants.
Article
Plant Sciences
Hannah Elisa Krawczyk, Alexander Helmut Rotsch, Cornelia Herrfurth, Patricia Scholz, Orr Shomroni, Gabriela Salinas-Riester, Ivo Feussner, Till Ischebeck
Summary: After reaching the stigma, pollen grains form a pollen tube to transport the sperm cells to the ovule. In response to heat stress, tobacco pollen tubes quickly remodel their lipidome and show metabolomic adaptations and transcriptional adjustments. These adaptations may be achieved through post-transcriptional and/or post-translational regulation.