Article
Biochemistry & Molecular Biology
Parool Gupta, Rani Mansuri, Priya Priydarshni, Sachidananda Behera, Amir Zaidi, Shamshun Nehar, Ganesh Chandra Sahoo, Krishna Pandey, Vahab Ali
Summary: This study revealed the interaction between two proteins, Cfd1 and Nbp35, in L. donovani, forming a stable complex involved in the cellular process of Fe-S cluster biogenesis and transfer. The upregulation of Cfd1 and Nbp35 proteins in drug-resistant parasites leads to increased Fe-S protein levels, promoting parasite survival under drug pressure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Inorganic & Nuclear
Corinne Aubert, Pierre Mandin, Beatrice Py
Summary: Fe-S clusters are important cofactors for metalloproteins involved in various cellular processes. Eukaryotes have acquired Fe-S protein biogenesis systems from bacteria through endosymbiosis, with these systems localized in mitochondria and plastids. Bacteria also possess components similar to eukaryotic CIA factors involved in Fe-S protein biogenesis.
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Article
Biochemistry & Molecular Biology
Yi Tong Vincent Aw, Azadeh Seidi, Jenni A. Hayward, Jiwon Lee, F. Victor Makota, Melanie Rug, Giel G. van Dooren
Summary: Fe-S clusters are essential for the survival of eukaryotes, with independent biosynthesis pathways occurring in different compartments of the cell. Little is known about cytosolic Fe-S cluster biosynthesis in apicomplexan parasites, but it has been found that NBP35 in Toxoplasma gondii localizes to the outer mitochondrial membrane and is critical for parasite proliferation.
MOLECULAR MICROBIOLOGY
(2021)
Review
Plant Sciences
Diego F. Gomez-Casati, Maria Busi, Julieta Barchiesi, Maria A. Pagani, Noelia S. Marchetti-Acosta, Agustina Terenzi
Summary: Iron and sulfur are essential elements for organisms, forming Fe-S clusters that play key roles in various cellular processes. Information on genes and proteins involved in Fe-S cluster assembly in green algae, particularly in mitochondrial pathways, is limited and requires further investigation.
Article
Biochemistry & Molecular Biology
Zhuanzhuan Liu, Shihong Xu, Zhiwei Zhang, Hanying Wang, Qiyue Jing, Shenghan Zhang, Mengnan Liu, Jinzhi Han, Yanbo Kou, Yanxia Wei, Lu Wang, Yugang Wang
Summary: FAM96A plays a crucial role in regulating Fe metabolism and obesity development, maintaining intracellular and systemic Fe homeostasis. Loss of FAM96A leads to adipocyte hypertrophy and reduction in organismal energy expenditure.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Plant Sciences
Styliani N. Chorianopoulou, Dimitris L. Bouranis
Summary: Sulfur is an essential macronutrient for plant growth and metabolism, and it plays diverse roles in their life cycles. It is connected to the homeostasis of essential micronutrients, such as iron, copper, zinc, and manganese. Sulfur is involved in the uptake, transportation, and distribution of these micronutrients, and it interacts with them in functional ways.
Article
Biochemistry & Molecular Biology
Tamara Smutna, Alena Dohnalkova, Robert Sutak, Ravi Kumar Narayanasamy, Jan Tachezy, Ivan Hrdy
Summary: This study discovered a previously unknown hydrogenase in the cytosol of T. vaginalis, which can use cytochrome b5 as an electron acceptor. Trichomonads with overexpressed cytosolic hydrogenase showed decreased hydrogen excretion and increased excretion of methylated alcohols, suggesting that the cytosolic hydrogenase uses hydrogen gas as a source of reducing power for cytoplasmic reactions.
Article
Plant Sciences
Ming-Der Huang, Chin-Wei Wu, Hong-Yun Chou, Sou-Yu Cheng, Hsin-Yang Chang
Summary: A novel nsLTP lineage is identified in green algae, which carries unique sequences and molecular features distinguishable from those in land plants. Further investigation on the Chlamydomonas nsLTPs shows their late zygotic stage expression patterns and verified canonical nsLTP properties such as fatty acids binding and proteinase resistance activities. This study not only reveals the existence of nsLTPs in green algae but also contributes to facilitating future studies on this enigmatic protein family.
Article
Biochemistry & Molecular Biology
Andrea Bombana, Muralidharan Shanmugam, David Collison, Alexander J. J. Kibler, Graham N. N. Newton, Christof M. M. Jager, Anna K. K. Croft, Simone Morra, Nicholas J. J. Mitchell
Summary: 'Bacterial-type' ferredoxins have a cluster that enables electron transfer and biological processes. Peptide maquettes based on the cluster-forming motif have been used as ferredoxin models. This study explores the integration of a [4Fe4S]-peptide maquette into a H-2-powered electron transport chain and demonstrates its reconstitution under aerobic conditions. The integration of these artificial metalloproteins into a semi-synthetic electron transport chain is also demonstrated.
Article
Biochemistry & Molecular Biology
Wen Gu, Carine Fillebeen, Kostas Pantopoulos
Summary: This study provides the first experimental evidence for the expression of nuclear IRP1 in mammalian cells, which appears to be species- and cell-specific. Furthermore, the nuclear translocation of IRP1 is mediated by an iron-dependent mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Rongrong Li, Hangjia Shen, Erum Pervaiz, Minghui Yang
Summary: In this study, an economical, efficient, and green FeS/N-C sulfur host material has been successfully synthesized. It avoids the use of hazardous heavy metals, reduces costs, and achieves extraordinary electrochemical performance with high sulfiphility.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Nihar Ranjan Prusty, Francesca Camponeschi, Simone Ciofi-Baffoni, Lucia Banci
Summary: Iron-sulfur (Fe-S) clusters are versatile cofactors in biology, with the biogenesis of Fe-S proteins in the cytosol assisted by the cytosolic Fe-S protein assembly machinery, which includes proteins such as human ORAOV1 and YAE1. Structural and cluster binding studies showed that ORAOV1 and the YAE1-ORAOV1 complex can bind a [4Fe-4S](2+) cluster, suggesting their potential role in Fe-S cluster insertion into ABCE1.
INORGANICA CHIMICA ACTA
(2021)
Article
Microbiology
Parikshit M. Bagchi, Kaitlyn J. Speckhart, Andrew M. Kennedy, Andrew J. Tai, Billy M. Tsai
Summary: This study identifies a host factor, EMC4, that promotes fusion of DENV and endosomal membranes during viral entry. These findings provide insights into the early entry steps of DENV and suggest a potential target for antiviral drug development.
Article
Genetics & Heredity
Jennifer H. Yang, Marisa W. Friederich, Katarzyna A. Ellsworth, Aliya Frederick, Emily Foreman, Denise Malicki, David Dimmock, Jerica Lenberg, Chitra Prasad, Andrea C. Yu, C. Anthony Rupar, Robert A. Hegele, Kandamurugu Manickam, Daniel C. Koboldt, Erin Crist, Samantha S. Choi, Sali M. K. Farhan, Helen Harvey, Shifteh Sattar, Natalya Karp, Terence Wong, Richard Haas, Johan L. K. Van Hove, Kristen Wigby
Summary: Iron-sulfur cluster proteins play critical roles in gene expression regulation and mitochondrial bioenergetics. The study reveals that a specific variant in the NFS1 gene can cause mitochondrial complex II and III deficiency, leading to various clinical presentations. These findings provide important insights into the underlying mechanisms of such disorders.
Review
Biochemistry & Molecular Biology
Joseph J. Braymer, Sven A. Freibert, Magdalena Rakwalska-Bange, Roland Lill
Summary: Iron-sulfur (Fe/S) proteins play essential roles in various cellular processes and their synthesis requires assistance from specific protein machineries. These systems differ between prokaryotes and eukaryotes, underlining the complexity and specificity of Fe/S protein biogenesis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Microbiology
Yueling Qi, Adam Ossowicki, Etienne Yergeau, Gianpiero Vigani, Violette Geissen, Paolina Garbeva
Summary: Plastic mulch film residues did not significantly impact soil suppressiveness level in the short term, but created a new niche plastisphere housing a unique microbial community dominated by potential fungal pathogens.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Review
Plant Sciences
Irene Murgia, Francesca Marzorati, Gianpiero Vigani, Piero Morandini
Summary: This review examines the influence of rhizosphere microbiota on iron uptake by plants and the process of iron loading into seeds. It also discusses the potential genetic resources provided by wild relatives for crop improvement.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Marco Herfurth, Anke Treuner-Lange, Timo Glatter, Nadine Wittmaack, Egbert Hoiczyk, Antonio J. Pierik, Lotte Sogaard-Andersen
Summary: The study reveals the formation of complexes between minor pilins and PilY1.1, contingent on calcium and a non-canonical cytochrome c, in Myxococcus xanthus. This discovery highlights the importance of these complexes in stabilizing PilY1.1 and enabling T4aP function in a broader range of calcium concentrations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Dario Di Silvestre, Giulia Passignani, Rossana Rossi, Marina Ciuffo, Massimo Turina, Gianpiero Vigani, Pier Luigi Mauri
Summary: A newly discovered plant mitovirus activates metabolic processes in quinoa to help them withstand drought, providing a new perspective for breeding crop plants. The study found changes in mitochondrial protein expression in infected plants, with up-regulation of functional modules involved in stress response and redox homeostasis, suggesting a potential mechanism for the observed higher tolerance to drought in infected plants. Further in-depth functional studies are needed to establish a causative link.
Review
Agronomy
Oussama Bouaicha, Tanja Mimmo, Raphael Tiziani, Nadine Praeg, Carlo Polidori, Luigi Lucini, Gianpiero Vigani, Roberto Terzano, Juan C. Sanchez-Hernandez, Paul Illmer, Stefano Cesco, Luigimaria Borruso
Summary: This review discusses the impact of microplastics (MP) on rhizosphere ecology, specifically focusing on the effects of MP on soil physical-chemical properties, plant physiology, and soil biodiversity. The long-term effects of MP on the interactions among soil organisms and plants are still not well studied, highlighting the need for further research on rhizosphere-MP interactions at a functional level.
Article
Biochemistry & Molecular Biology
Ulrich Muehlenhoff, Benjamin Dennis Weiler, Franziska Nadler, Robert Millar, Isabell Kothe, Sven-Andreas Freibert, Florian Altegoer, Gert Bange, Roland Lill
Summary: Mitochondrial Iba57 protein is responsible for the assembly of [4Fe-4S] proteins. Through studies in yeast, it was found that the function of Iba57 is independent of folate, contrary to previous assumptions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Vinzent Schulz, Somsuvro Basu, Sven-A Freibert, Holger Webert, Linda Boss, Ulrich Muehlenhoff, Fabien Pierrel, Lars-O Essen, Douglas M. Warui, Squire J. Booker, Oliver Stehling, Roland Lill
Summary: This study identifies new biochemical functions of mitochondrial ferredoxins and provides structural insights into their functional specificity.
NATURE CHEMICAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Vinzent Schulz, Sven-A Freibert, Linda Boss, Ulrich Muehlenhoff, Oliver Stehling, Roland Lill
Summary: This review focuses on the structure, function and specificity of mitochondrial [2Fe-2S] FDXs and their important roles in various biological processes. Mitochondrial FDXs play crucial functions in steroid transformations, Fe/S protein biogenesis, and cofactor biosynthesis through electron transfer.
Article
Biochemistry & Molecular Biology
Marion Jespersen, Antonio J. Pierik, Tristan Wagner
Summary: Methanogenic archaea play a crucial role in the carbon cycle but are sensitive to reactive sulfite. Researchers have discovered a sulfite detoxification system in some methanogens, known as coenzyme F-420-dependent sulfite reductase (Group I Fsr), which combines an F420H2-oxidase with a sulfite reductase. The structural analysis of Fsr from marine methanogens revealed a unique fusion of a primitive sulfite reductase that converts a poison into an essential component of life.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Felipe A. Carvalho, Ulrich Muehlenhoff, Joseph J. Braymer, Vasilij Root, Martin Stuempfig, Carla C. Oliveira, Roland Lill
Summary: Systematic studies have shown that the Hsp90 chaperone system and Fe/S protein biogenesis or iron regulation are interconnected. DJA5 and DJA6 are two chloroplast-localized proteins that act as iron donors in plastidial Fe/S protein biogenesis. In this study, the impact of Hsp90 chaperone, yeast DJA5-DJA6 homologs, cytosolic Ydj1, and mitochondrial Mdj1 on cellular iron-related processes was investigated using Saccharomyces cerevisiae. Depletion of these crucial proteins resulted in severe phenotypes, but did not have a critical in vivo impact on Fe/S protein biogenesis or iron regulation. Unlike plant DJA5-DJA6 iron chaperones, Ydj1 and Mdj1 did not bind iron in vivo, suggesting that zinc is used for their function under normal physiological conditions.
Article
Plant Sciences
Matteo Chialva, Davide Lucien Patono, Leonardo Perez de Souza, Mara Novero, Sara Vercellino, Moez Maghrebi, Michele Morgante, Claudio Lovisolo, Gianpiero Vigani, Alisdair Fernie, Valentina Fiorilli, Luisa Lanfranco, Paola Bonfante
Summary: Coffee, being one of the most traded commodities globally, exhibits a strong interaction with arbuscular mycorrhizal (AM) fungi. This study explored the mycorrhizal phenotype of two important coffee cultivars under phosphorus limitation and found that fungal colonization significantly increased biomass, photosynthetic efficiency, and plant nutrition. This interaction also led to reorganization of major metabolic pathways.
Article
Plant Sciences
Francesca Secchi, Ivan Bevilacqua, Chiara Agliassa, Moez Maghrebi, Silvia Cavalletto, Cristina Morabito, Silvia Lembo, Gianpiero Vigani
Summary: This study aimed to investigate the physiological and biochemical responses of Populus nigra plants under drought and recovery, grown in soil with impaired nutrient bioavailability obtained by adding calcium oxide (CaO) to the substrate. Although the CaO treatment did not affect plant growth, treated poplars displayed an impaired inorganic ions profile in tissues under well-watered conditions. During water stress relief, the CaO-treated poplars exhibited a faster stomatal opening and a higher capacity to restore xylem hydraulic conductivity compared to not-treated plants, probably due to the higher osmolyte accumulation during drought. Our results suggest that CaO treatment promotes a faster and more efficient plant recovery after drought due to a modulation of ions homeostasis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Carola S. Seelmann, Simona G. Huwiler, Martin Culka, Marc J. F. Strampraad, Till Biskup, Stefan Weber, G. Matthias Ullmann, Volker Schunemann, Peter-Leon Hagedoorn, Antonio J. Pierik, Matthias Boll
Summary: In anaerobic aromatic compound degrading microbes, class II benzoyl-coenzyme A (CoA) reductases (BCRs) reduce benzoyl-CoA to cyclohexa-1,5-diene-1-carboxyl-CoA (1,5-dienoyl-CoA) through a tungsten-bis-metallopterin (MPT) cofactor. This study provides experimental evidence for the Birch-like reduction mechanism via W(V)/radical intermediates and reveals the unique tetrahydro state of MPT cofactors essential for the reversibility of enzymatic Birch reduction.
Article
Biology
Jonathan Fuchs, Rapolas Jamontas, Maren Hellen Hoock, Jonathan Oltmanns, Beatrice Golinelli-Pimpaneau, Volker Schuenemann, Antonio J. Pierik, Rolandas Meskys, Agota Aucynaite, Matthias Boll
Summary: This study reveals a new enzyme called TudS desulfidase in cells, which can desulfurize thiouracil and play a crucial role in recycling and detoxification of tRNA-derived 4-thiouridine monophosphate nucleosides for RNA synthesis.
COMMUNICATIONS BIOLOGY
(2023)
