Article
Biochemistry & Molecular Biology
Robert Czajkowski, Lukasz Rabalski, Maciej Kosinski, Eigil de Neergaard, Susanne Harding
Summary: Pectobacterium atrosepticum is a plant-pathogenic bacterium causing blackleg of potato worldwide. A high-quality genome of the P. atrosepticum strain Green1 isolated from potato plants in Greenland under a subarctic climate was presented, which provides resources for comparative genomic studies and analyses targeting climatic adaptation and ecological fitness mechanisms.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Microbiology
Giarla Cunha da Silva, Jessica Nogueira Rosa, Patricia Pereira Fontes, Alex Gazolla de Castro, everton De Almeida Alves Barbosa, Wellington Ronildo Clarindo, Hilario Cuquetto Mantovani, Yanwen Li, Janine Therese Bosse, Paul Richard Langford, Denise Mara Soares Bazzolli
Summary: EVs produced by Actinobacillus pleuropneumoniae contain sRNAs, including those involved in pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Umang Dhaubhadel, Abiud E. Portillo, Ondrej Horacek, Yu-Sheng Sung, Daniel W. Armstrong
Summary: Quorum Sensing is a mechanism that allows bacteria to sense their population density through a signaling molecule called N-acyl homoserine lactone (N-HL). This study reports the production of D-N-HLs by the plant pathogen Pectobacterium atrosepticum and the human pathogen Pseudomonas aeruginosa. Interestingly, both bacteria produced significant amounts of D-N-HLs, some of which were higher in concentration compared to the commonly studied L-N-HLs. This work also discovered previously unreported D-enantiomers of N-3-oxoacyl and N-3-hydroxyacyl homoserine lactones. The significance and potential sources of D-N-HLs are discussed.
Review
Plant Sciences
Quan Zeng, Joanna Pulawska, Jeffrey Schachterle
Summary: This review examines the different infection stages of the fire blight disease caused by Erwinia amylovora, focusing on the early events during the initial infection of flowers and shoot tips. It discusses the production and transfer of inoculum, epiphytic proliferation of the pathogen, entry into the host, and initial proliferation inside the host. Additionally, the review delves into how E. amylovora modulates its virulence expression to induce disease symptoms during these early events.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Food Science & Technology
Usman Pato, Emma Riftyan, Nia N. Jonnaidi, Mimi S. Wahyuni, Jeska A. Feruni, Mosaad A. Abdel-Wahhab
Summary: This study evaluated the antimicrobial efficiency and stability of bacteriocins produced by dadih's LAB against Erwinia carotovora under different pH, temperature, and enzymatic treatments. The results demonstrated that certain bacteriocins showed high stability under various pH and temperature conditions.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Microbiology
Johannes Mapuranga, Jiaying Chang, Lirong Zhang, Na Zhang, Wenxiang Yang
Summary: Fungal plant pathogens employ proteinaceous and non-proteinaceous effectors, including secondary metabolites (SMs) and small non-coding RNAs (sRNAs), to manipulate the host plant's defense system. While much research has focused on secreted effector proteins, the roles of fungal SMs and sRNAs have been largely overlooked. Fungal SMs are generated during plant colonization, especially in early infection stages, while fungal sRNAs target plant genes involved in resistance and defense responses. Understanding the functions of these effectors can provide insights into the infection process and host genes targeted. This review discusses the roles of fungal SMs and sRNAs in plant-fungal interactions, translocation of sRNA effectors across kingdoms, and the application of cross-kingdom RNA interference in managing plant diseases.
Review
Dentistry, Oral Surgery & Medicine
P. D. Veith, M. D. Glew, D. G. Gorasia, E. Cascales, E. C. Reynolds
Summary: Bacteria in severe periodontitis utilize the T9SS system to release virulence factors that dysregulate the host immune response, leading to chronic inflammatory destruction of tooth supporting tissues. The molecular structure and secretion mechanism of T9SS are crucial for understanding this process.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Microbiology
Nicholas L. Xerri, Shelley M. Payne
Summary: The study revealed that the conditioned growth medium from the commensal bacterium Bacteroides thetaiotaomicron represses the invasion of Shigella flexneri. This repression is attributed to the presence of B. thetaiotaomicron outer membrane vesicles, indicating a novel role of bacterial outer membrane vesicles in modulating pathogenicity.
Article
Microbiology
Pablo Farace, Silvio Cravero, Catalina Taibo, Julian Diodati, Claudia Morsella, Fernando Paolicchi, Julia Sabio y Garcia, Andrea Gioffre
Summary: This study evaluated and characterized the secretion of outer membrane vesicles (OMVs) in Campylobacter fetus, confirming their production in all tested strains. The OMVs showed a spherical shape and variable size, and immunoreactivity was demonstrated. These OMVs could potentially modulate host response and be used as adjuvants in veterinary vaccines.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2022)
Article
Food Science & Technology
Maria Fernanda Ruiz-Cisneros, Jose de Jesus Ornelas-Paz, Guadalupe Isela Olivas-Orozco, Carlos Horacio Acosta-Muniz, Miguel Angel Salas-Marina, Francisco Javier Molina-Corral, David Ignacio Berlanga-Reyes, Sylvia Patricia Fernandez-Pavia, Octavio Jhonathan Cambero-Campos, Claudio Rios-Velasco
Summary: This study investigated the effect of Bacillus strains on the production of human health-related compounds and volatile organic compounds (VOCs) in tomato fruits. The results showed that Bacillus strains increased the carotenoid and phenol contents in tomatoes, while phytopathogens decreased these contents. Both Bacillus strains and pathogens increased the antioxidant capacity in tomatoes. The concentration of VOCs in tomatoes also increased when inoculated with Bacillus strains.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stephanie Marsin, Yazid Adam, Claire Cargemel, Jessica Andreani, Sonia Baconnais, Pierre Legrand, Ines Li de la Sierra-Gallay, Adeline Humbert, Magali Aumont-Nicaise, Christophe Velours, Francoise Ochsenbein, Dominique Durand, Eric Le Cam, Helene Walbott, Christophe Possoz, Sophie Quevillon-Cheruel, Jean-Luc Ferat
Summary: The study shows that VcDciA can stimulate the loading of VcDnaB on DNA, resulting in increased DNA unwinding. This work is the first step towards understanding the ancestral mode of loading bacterial replicative helicases on DNA.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
J. C. Amaro-Hernandez, G. I. Olivas, C. H. Acosta-Muniz, N. Gutierrez-Mendez, C. Rios-Velasco, D. R. Sepulveda
Summary: Rennet milk curds were prepared under different temperature and acidity conditions, and the formation of various types of inter-protein chemical bonds was monitored. The results showed that hydrophobic interactions were replaced by electrostatic, hydrogen, and calcium bonds, while disulfide bonds remained constant. Acidification increased the availability of soluble calcium ions, leading to an increase in calcium bridges and a decrease in electrostatic-hydrogen bonds. pH determined the nature of protein interactions, while temperature modified the rate at which these bonds formed.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Genetics & Heredity
Alexia L. Carboni, Mark A. Hanson, Scott A. Lindsay, Steven A. Wasserman, Bruno Lemaitre
Summary: Cecropins are small helical secreted peptides with antimicrobial activity that play an important role in host defense. Experimental studies have shown that Cecropins play a role in defense against Gram-negative bacteria and fungi in fruit flies.
Article
Biochemistry & Molecular Biology
Claire Cargemel, Helene Walbott, Dominique Durand, Pierre Legrand, Malika Ouldali, Jean-Luc Ferat, Stephanie Marsin, Sophie Quevillon-Cheruel
Summary: DNA replication requires unwinding by replicative helicase. Our study discovers a new structure of bacterial helicase DnaB, which is a labile and inactive hexamer, representing an intermediate state for the active hexamer formation.
Article
Multidisciplinary Sciences
Prashant P. Damke, Louisa Celma, Sumedha M. Kondekar, Anne Marie Di Guilmi, Stephanie Marsin, Jordane Depagne, Xavier Veaute, Pierre Legrand, Helene Walbott, Julien Vercruyssen, Raphael Guerois, Sophie Quevillon-Cheruel, J. Pablo Radicella
Summary: The ComFC protein is essential for natural transformation and is involved in transporting DNA through the cell membrane and handling single-stranded DNA in the cytoplasm. The crystal structure of ComFC and its affinity for single-stranded DNA suggest that it plays a crucial role in the recombination machinery.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Daniel Alonso Perez-Corral, Jose de Jesus Ornelas-Paz, Guadalupe Isela Olivas, Carlos Horacio Acosta-Muniz, Miguel Angel Salas-Marina, David Ignacio Berlanga-Reyes, David Roberto Sepulveda, Yericka Mares-Ponce de Leon, Claudio Rios-Velasco
Summary: This study evaluated the abilities of four antifungal Streptomyces strains to produce plant growth substances, solubilize phosphates, and fix nitrogen, as well as the effects of their emitted volatile organic compounds (VOCs) on the growth of Arabidopsis thaliana and Phaseolus vulgaris seedlings. The results showed that all Streptomyces strains produced indole-3-acetic acid (IAA) and solubilized phosphates, but did not fix nitrogen. The VOCs from Streptomyces promoted the growth of A. thaliana and had significant effects on P. vulgaris seedlings.
Article
Multidisciplinary Sciences
Florent Masson, Samuel Rommelaere, Fanny Schupfer, Jean-Philippe Boquete, Bruno Lemaitre
Summary: The abundance and amino acid composition of a protein called Spiralin B (SpiB) plays a crucial role in the nutritional interactions between insects and their endosymbionts. Increasing SpiB levels disrupts the localization of endosymbionts in insect eggs and decreases vertical transmission. This protein ensures the durability of the interaction in a variable environment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
M. A. Hanson, S. Kondo, B. Lemaitre
Summary: Antimicrobial peptides (AMPs) play a crucial role in defending against infection in plants and animals. A study on Drosophila has revealed that the Drosocin gene encodes two prominent host defense peptides with different specificities against distinct pathogens.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Genetics & Heredity
Mark Hanson, Bruno Lemaitre
Summary: Antimicrobial peptides (AMPs) are host-encoded antibiotics that not only combat invading pathogens, but also have roles in neurological contexts. The evolutionary history of Baramicin genes reveals how they have adapted for either immune-specific or neurological roles, providing a case study for how AMP-encoding genes might play dual roles in both immune and non-immune processes via their multiple peptide products.
Review
Agriculture, Multidisciplinary
Daniel Alejandro de La Pena-Baca, Alejandro Romo-Chacon, Claudio Rios-Velasco, Guadalupe Isela Olivas-Orozco, Jose de Jesus Ornelas-Paz, Carlos Horacio Acosta-Muniz
Summary: Fire blight is a plant disease caused by the bacterium Erwinia amylovora. The primary sources of infection include overwintering cankers, as well as shoots, fruits, and non-host plants. The bacterium can enter a viable but non-culturable state, making it difficult to detect. This review discusses the sources of infection and the survival mechanism of E. amylovora.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Biochemistry & Molecular Biology
Claire Cargemel, Sonia Baconnais, Magali Aumont-Nicaise, Magali Noiray, Lia Maurin, Jessica Andreani, Helene Walbott, Eric Le Cam, Francoise Ochsenbein, Stephanie Marsin, Sophie Quevillon-Cheruel
Summary: This study demonstrates that the bacterial replicative helicase loader DciA interacts with single-stranded and double-stranded DNA in an atypical mode, potentially acting as a DNA chaperone. Point mutations in DciA's long alpha-helix 1 affect its interaction with different DNA substrates and the loading of the helicase. These findings suggest that direct interaction between DciA and DNA may play a role in the helicase loading mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Claire Cargemel, Stephanie Marsin, Magali Noiray, Pierre Legrand, Halil Bounoua, Ines Li De la Sierra-Gallay, Helene Walbott, Sophie Quevillon-Cheruel
Summary: During bacterial genome replication initiation, replicative helicases rely on specialized proteins for their loading onto oriC. DnaC and DnaI were the first loaders to be characterized. However, many bacteria do not have these genes and instead have domesticated phage elements that have replaced the original loader gene dciA. A crystal structure study of the complex from Vibrio cholerae revealed that two VcDciA molecules interact with a dimer of VcDnaB without altering its canonical structure, providing insights into how DciA assists in DnaB loading. Surprisingly, DnaC from Escherichia coli also targets the same module on EcDnaB, suggesting functional interchangeability between VcDciA and EcDnaC despite their lack of structural similarity. This study represents a significant advancement in understanding the mechanism by which phage helicase loaders hijack bacterial replicative helicases during evolution.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Cell Biology
Mark A. Hanson, Bruno Lemaitre
Summary: Antimicrobial peptides (AMPs) play important roles in host defense and have been implicated in the clearance of aberrant cells and neurodegenerative syndromes. AMPs are produced in Drosophila downstream of Toll and Imd NF-κB pathways and have antimicrobial properties. Aging leads to upregulation of AMPs, potentially contributing to age-associated inflammatory diseases. However, the individual effects of AMPs on lifespan have been inconclusive. In this study, the researchers found no significant effect of individual AMPs on lifespan, except for Defensin. However, flies lacking seven AMP gene families displayed a reduced lifespan due to microbiome dysbiosis, and germ-free conditions extended their lifespan. Overall, AMPs collectively impact lifespan by preventing dysbiosis during aging.
DISEASE MODELS & MECHANISMS
(2023)
Article
Multidisciplinary Sciences
M. A. Hanson, L. Grollmus, B. Lemaitre
Summary: Antimicrobial peptides, encoded by the host, play a crucial role in combating pathogens and shaping the microbiome in plants and animals. This study focuses on the Diptericin antimicrobial peptide family of Diptera and reveals the specific roles of DptA and DptB in Drosophila melanogaster, demonstrating how they interact with different bacteria. The findings also highlight the correlation between the presence of DptA- or DptB-like genes and the presence of specific bacteria across Diptera.