Article
Chemistry, Multidisciplinary
Kathleen A. Trychta, Bing Xie, Ravi Kumar Verma, Min Xu, Lei Shi, Brandon K. Harvey
Summary: Recent research shows that the carboxy tail sequences of ER resident proteins can stably bind to Golgi KDELRs, not limited to the classic KDEL sequence, aiding in predicting whether ER resident proteins will undergo secretion in response to ER calcium depletion.
FRONTIERS IN CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varo'n Silva, Peter H. Seeberger, Yoshiko Murakami, Taroh Kinoshite
Summary: Researchers have identified a protein called CLPTM1L that plays a crucial role in the synthesis of glycosylphosphatidylinositols (GPIs), complex glycolipids involved in cell surface protein anchoring. CLPTM1L acts as a scramblase, facilitating the translocation of GlcN-PI from the endoplasmic reticulum to the cytosol for efficient GPI biosynthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Garyfallia Gouna, Christian Klose, Mar Bosch-Queralt, Lu Liu, Ozgun Gokce, Martina Schifferer, Ludovico Cantuti-Castelvetri, Mikael Simons
Summary: Microglia play a key role in promoting myelin repair after demyelinating injury, with cholesterol esterification being essential for myelin debris clearance and lipid droplet generation. TREM2 is crucial for adapting to excess cholesterol exposure and regulating lipid droplet formation, ultimately impacting the innate immune response and remyelination.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Linhui Yu, Jilian Fan, Chao Zhou, Changcheng Xu
Summary: Lipid droplet biogenesis occurs at specific sites in the endoplasmic reticulum and is regulated by a specific set of lipids and proteins. Sterols are shown to play a crucial role in coordinating oil and oleosin biosynthesis for the formation of lipid droplets in plants such as Arabidopsis.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yuanjiao Du, Weiping Chang, Lei Gao, Lin Deng, Wei-Ke Ji
Summary: In this study, Tex2, a lipid transfer protein found on the tubular endoplasmic reticulum, was discovered to be recruited to late endosomes/lysosomes by TMEM55, phosphatases that convert PI(4,5)P-2 to PI5P on LE/lys. The interaction between Tex2 and TMEM55 is regulated by endosome-resident type 2 PI4K activities. Tex2 knockout results in defects in lysosomal trafficking, digestive capacity, and lipid composition of LE/lys membranes. These findings highlight the importance of Tex2 in maintaining lysosomal functions through its presence at TMEM55-dependent ER-LE/lys MCSs.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ning Wang, Yoko Shibata, Joao A. Paulo, Steven P. Gygi, Tom A. Rapoport
Summary: Rop1 is the only member of a subfamily of membrane-curvature generating REEPs in fission yeast. Wang et al. demonstrate that Rop1 is crucial for macroautophagy by facilitating autophagosome formation. Rop1 functions in the generation of high membrane curvature and colocalizes with the autophagy component Atg2 at the phagophore rim.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Wei-Chou Lin, Yen-Lin Chiu, Kuan-Lin Kuo, Po-Ming Chow, Chen-Hsun Hsu, Shih-Ming Liao, Jun-Ren Dong, Shih-Chen Chang, Shing-Hwa Liu, Tsai -Jung Liu, Fu -Shun Hsu, Kuo-Chuan Wang, Yi-Chih Lin, Chen-Chih Chang, Kuo-Yuan Huang
Summary: PR-619, a novel inhibitor of deubiquitinating enzymes, has been shown to have anti-tumor effects in various malignancies. This study investigated the impact of PR-619 on chondrosarcoma and found that it induced apoptosis, cell cycle arrest, and cellular and endoplasmic reticulum stress in chondrosarcoma cells. In a xenograft mouse model, PR-619 effectively inhibited tumor growth with minimal toxicity. These findings suggest that PR-619 could be a potential novel therapeutic strategy for chondrosarcoma.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Takuma Kozono, Hiroyuki Sato, Wataru Okumura, Chifuyu Jogano, Miwa Tamura-Nakano, Yuki Kawamura, Jack Rohrer, Takashi Tonozuka, Atsushi Nishikawa
Summary: The N-terminal region of Jaw1 inhibits the formation of organized smooth ER (OSER) and maintains the homeostatic localization of interactors on the ER membrane by preventing aberrant oligomerization as an intrinsically disordered region (IDR).
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Camilla Palumbo, Alice Mecchia, Alessio Bocedi, Katia Aquilano, Daniele Lettieri-Barbato, Marco Rosina, Almerinda Di Venere, Carlo Rodolfo, Anna Maria Caccuri
Summary: The study found that TRAF2 is dispensable for the activation of JNK and autophagy in HAP1 cells, while the TRAF2 C-terminal domain may play an important role in regulating the cellular response to ER stress.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Asako Goto, Shota Sakai, Aya Mizuike, Toshiyuki Yamaji, Kentaro Hanada
Summary: Casein kinase 1 gamma (CK1G) plays a role in regulating cellular functions and has been found to inactivate the ceramide transport protein (CERT) through phosphorylation. In this study, it was discovered that loss of the C-terminal region of CK1G3 led to hyperactivation of the kinase and redistribution within the cell. These findings suggest that CK1Gs are regulated through compartmentalization to access specific intracellular organelles.
Article
Endocrinology & Metabolism
Francesca Tonelli, Laura Leoni, Valentina Daponte, Roberta Gioia, Silvia Cotti, Imke A. K. Fiedler, Daria Larianova, Andy Willaert, Paul J. Coucke, Simona Villani, Bjoern Busse, Roberta Besio, Antonio Rossi, P. Eckhard Witten, Antonella Forlino
Summary: This study used zebrafish to investigate the role of TRIC-B in skeletal tissue. The researchers found that the tmem38b gene is expressed at early developmental stages in zebrafish, while tmem38a gene is not. Mutations in tmem38b resulted in under-modified collagen type I and intracellular retention. These findings contribute to our understanding of the importance of TRIC-B in bone cell differentiation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ziqi Ren, Ran Li, Xinyue Zhou, Yu Chen, Yuxin Fang, Peng Zou
Summary: In eukaryotic cells, RNA localization is crucial for cellular physiology. Recent discovery of RNA N-glycan modification challenges the traditional belief that RNA is excluded from secretory pathway compartments. This study provides evidence of small non-coding RNAs in the ER lumen and raises questions about their transport mechanism and biological functions.
Article
Plant Sciences
Xifeng Li, Zhe Wang, Yunting Fu, Xi Cheng, Yan Zhang, Baofang Fan, Cheng Zhu, Zhixiang Chen
Summary: UBAC2 is a multifunctional protein in plant cells that regulates the degradation and accumulation of specific proteins, including COPT and POWDERY MILDEW RESISTANCE 4.
Article
Chemistry, Medicinal
Kentaro Futatsugi, Shawn Cabral, Daniel W. Kung, Kim Huard, Esther Lee, Markus Boehm, Jonathan Bauman, Ronald W. Clark, Steven B. Coffey, Collin Crowley, Anne-Marie Dechert-Schmitt, Matthew S. Dowling, Robert Dullea, James R. Gosset, Amit S. Kalgutkar, Kou Kou, Qifang Li, Yajing Lian, Paula M. Loria, Allyn T. Londregan, Mark Niosi, Christine Orozco, John C. Pettersen, Jeffrey A. Pfefferkorn, Jana Polivkova, Trenton T. Ross, Raman Sharma, Ingrid A. Stock, Gregory Tesz, Hanna Wisniewska, Bryan Goodwin, David A. Price
Summary: This study describes the discovery of ervogastat, a first-in-class DGAT2 inhibitor, for the treatment of non-alcoholic steatohepatitis (NASH) with liver fibrosis. Through key design elements, potential development risks of the prototype drug were addressed.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Navit Ogen-Shtern, Chieh Chang, Haddas Saad, Niv Mazkereth, Chaitanya Patel, Marina Shenkman, Gerardo Z. Lederkremer
Summary: Misfolded proteins and components of the ER quality control and ERAD machineries accumulate in the pericentriolar ERQC, which serves as a staging ground for ERAD. Trafficking to the ERQC is reversible, with recycling back to the ER slower than movement in the ER periphery. Vesicular trafficking, rather than diffusion, is involved in this process. COPI inhibition leads to accumulation in the ERQC and increases ERAD, while COPII inhibition has the opposite effect. Targeting of misfolded proteins to ERAD involves COPII-dependent transport to the ERQC and retrieval to the peripheral ER in a COPI-dependent manner.
Article
Biophysics
Alexander Harder, Ann-Kristin Moeller, Fabian Milz, Phillipp Neuhaus, Volker Walhorn, Thomas Dierks, Dario Anselmetti
BIOPHYSICAL JOURNAL
(2015)
Editorial Material
Biochemistry & Molecular Biology
Jianhe Peng, Sarfaraz Alam, Karthikeyan Radhakrishnan, Malaiyalam Mariappan, Markus Georg Rudolph, Caroline May, Thomas Dierks, Kurt von Figura, Bernhard Schmidt
Article
Oncology
Susmita Mondal, Debarshi Roy, Juliana Camacho-Pereira, Ashwani Khurana, Eduardo Chini, Lifeng Yang, Joelle Baddour, Katherine Stilles, Seth Padmabandu, Sam Leung, Steve Kalloger, Blake Gilks, Val Lowe, Thomas Dierks, Edward Hammond, Keith Dredge, Deepak Nagrath, Viji Shridhar
Article
Cell Biology
Heike Wolf, Markus Damme, Stijn Stroobants, Rudi D'Hooge, Hans Christian Beck, Irm Hermans-Borgmeyer, Renate Luellmann-Rauch, Thomas Dierks, Torben Luebke
DISEASE MODELS & MECHANISMS
(2016)
Article
Endocrinology & Metabolism
Ilona Jaszczuk, Lars Schlotawa, Thomas Dierks, Andreas Ohlenbusch, Dominique Koppenhoefer, Mariusz Babicz, Monika Lejman, Karthikeyan Radhakrishnan, Agnieszka Lugowska
MOLECULAR GENETICS AND METABOLISM
(2017)
Article
Biochemistry & Molecular Biology
Bjoern Kowalewski, Peter Heimann, Theresa Ortkras, Renate Luellmann-Rauch, Tomo Sawada, Steven U. Walkley, Thomas Dierks, Markus Damme
HUMAN MOLECULAR GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Bjoern Kowalewski, Torben Luebke, Katrin Kollmann, Thomas Braulke, Thomas Reinheckel, Thomas Dierks, Markus Damme
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Juliane Poppe, Eberhard Warkentin, Ulrike Demmer, Bjoern Kowalewski, Thomas Dierks, Klaus Schneider, Ulrich Ermler
JOURNAL OF INORGANIC BIOCHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Ina Kalus, Susanne Rohn, Tania M. Puvirajesinghe, Scott E. Guimond, Pieter J. Eyckerman-Koelln, Gerdy ten Dam, Toin H. van Kuppevelt, Jeremy E. Turnbull, Thomas Dierks
Article
Multidisciplinary Sciences
Debarshi Roy, Susmita Mondal, Ashwani Khurana, Deok-Beom Jung, Robert Hoffmann, Xiaoping He, Eleftheria Kalogera, Thomas Dierks, Edward Hammond, Keith Dredge, Viji Shridhar
SCIENTIFIC REPORTS
(2017)
Article
Genetics & Heredity
Orna Staretz-Chacham, Lars Schlotawa, Ohad Wormser, Inbal Golan-Tripto, Ohad S. Birk, Carlos R. Ferreira, Thomas Dierks, Karthikeyan Radhakrishnan
MOLECULAR GENETICS & GENOMIC MEDICINE
(2020)
Review
Biochemistry & Molecular Biology
Lars Schlotawa, Laura A. Adang, Karthikeyan Radhakrishnan, Rebecca C. Ahrens-Nicklas
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Omkar P. Dhamale, Roger Lawrence, Elena M. Wiegmann, Bhahwal A. Shah, Kanar Al-Mafraji, William C. Lamanna, Torben Luebke, Thomas Dierks, Geert-Jan Boons, Jeffrey D. Esko
ACS CHEMICAL BIOLOGY
(2017)
Article
Ophthalmology
Katharina Kruszewski, Renate Luellmann-Rauch, Thomas Dierks, Udo Bartsch, Markus Damme
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)