Review
Cell Biology
Nhat-Tu Le
Summary: ERK5, a member of the MAPK family, has distinct activation mechanisms and catalytic-independent functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Simon T. T. Abrams, Yasir Alhamdi, Min Zi, Fengmei Guo, Min Du, Guozheng Wang, Elizabeth J. J. Cartwright, Cheng-Hock Toh
Summary: Reduction in cardiac contractility is common in severe sepsis, and the mechanism behind it is still not fully understood. Recent findings suggest that circulating histones released after immune cell death play a role in cardiomyocyte injury and contractility reduction. However, the specific mechanism by which histones cause cardiac dysfunction is still unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jianlin Lu, Yuanyuan Huang, Li Zhan, Ming Wang, Leilei Xu, McKay Mullen, Jianye Zang, Guowei Fang, Zhen Dou, Xing Liu, Wei Liu, Minerva Garcia-Barrio, Xuebiao Yao
Summary: This study reveals a specific signaling cascade involving sequential phosphorylation by CDK1 and PLK1 of AMPK alpha 2 in mitosis, which plays a crucial role in maintaining genomic stability by accurately guiding chromosome segregation. This isoform-specific function of AMPK alpha 2 will provide valuable insights for future research on energy sensing in mitosis.
Article
Multidisciplinary Sciences
Kouki Kawakami, Masataka Yanagawa, Suzune Hiratsuka, Misaki Yoshida, Yuki Ono, Michio Hiroshima, Masahiro Ueda, Junken Aoki, Yasushi Sako, Asuka Inoue
Summary: This study uncovers the role of Gq heterotrimer in determining GRK subtype selectivity, which in turn regulates the conformation and functionality of β-arrestin. By investigating the angiotensin II type-1 receptor, the authors find that both GRK2/3 and GRK5/6 are recruited by Ang II, while only GRK5/6 is recruited by the β-arrestin-biased ligand TRV027. Additionally, inhibition of Gq shifts the GRK subtype selectivity and β-arrestin functionality to that of TRV027.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lorena Agudo-Ibanez, Marta Morante, Lucia Garcia-Gutierrez, Andrea Quintanilla, Javier Rodriguez, Alberto Munoz, Javier Leon, Piero Crespo
Summary: The transcription factor MYC is regulated by the kinase activity of ERK2 and plays a role in cell proliferation, transformation, and survival. ERK2 was found to directly regulate MYC transcription by binding to the MYC promoter. ERK2 increased MYC expression and protein abundance in the nucleus, independent of its kinase activity, through interactions with CDK9. These findings demonstrate a kinase-independent role for ERK2 in promoting MYC expression by anchoring CDK9 to the MYC promoter.
Article
Cell Biology
Angela Flavia Serpico, Francesco Febbraro, Caterina Pisauro, Domenico Grieco
Summary: This research highlights the compartmentalized control of Cdk1 activity during mitosis, where the inhibitory fraction of Cdk1 (i-Cdk1) plays a crucial role in spindle assembly.
Article
Cell Biology
Ruizhi Li, Kristin F. Wilson, Richard A. Cerione
Summary: ATF4 is a key stress-responsive transcription factor that aids cancer cells in surviving oxidative stress, nutrient deprivation, and matrix detachment. The mTORC2-PKC-Nrf2 pathway and Sirt5 are crucial for sustaining ATF4 response.
CELL DEATH DISCOVERY
(2022)
Article
Pharmacology & Pharmacy
Shotaro Michinaga, Ayaka Nagata, Ryosuke Ogami, Yasuhiro Ogawa, Shigeru Hishinuma
Summary: This study aimed to explore how H1 receptor-mediated ERK phosphorylation is regulated by Gq proteins and arrestins. The results showed that the Gq protein pathway mainly regulates early phosphorylation reaction, while the arrestin pathway mainly regulates late phosphorylation reaction.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Takefumi Kimura, Sai P. Pydi, Lei Wang, Dhanush Haspula, Yinghong Cui, Huiyan Lu, Gabriele M. Koenig, Evi Kostenis, Gregory R. Steinberg, Oksana Gavrilova, Juergen Wess
Summary: This study found that by activating adipocyte G(q) signaling, the increased lipolysis in obese individuals can be prevented, leading to improved glucose and lipid homeostasis. This has important implications for the development of novel anti-diabetic drugs based on GPCR.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Moshe T. Gordon, Brian P. Ziemba, Joseph J. Falke
Summary: This study investigates the association-dissociation kinetics of the PDK1:PKCa heterodimer. The findings reveal that single PDK1 molecules freely diffusing on the membrane surface undergo reversible switching between the PDK1 monomer and the PDK1:PKCa heterodimer. The elucidation of these kinetics has important biological and medical implications.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
John E. Chojnowski, Rongrong Li, Tiffany Tsang, Fatimah H. Alfaran, Alexej Dick, Simon Cocklin, Donita C. Brady, Todd I. Strochlic
Summary: Casein kinase 2 (CK2) is an evolutionarily conserved enzyme involved in cellular functions, and its dysregulation is linked to diseases like cancer. This study demonstrates that copper enhances CK2 activity by binding directly to the enzyme. Specific residues in the catalytic subunit are critical for copper-binding. Changes in cellular copper levels also affect CK2 activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Cell Biology
Li-Min Mao, Nirav Mathur, Tayyibah Mahmood, Sri Rajan, Xiang-Ping Chu, John Q. Wang
Summary: This review summarizes recent progress in research on phosphorylation and phosphorylation-dependent regulation of group II metabotropic glutamate receptors. It includes detailed mapping of phosphorylation sites and the roles of protein kinases and phosphatases. Additionally, the potential linkage of mGlu2/3 phosphorylation to neurological and neuropsychiatric disorders is explored, and future research directions are discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eunyoung Lee, Zhenan Liu, Nhu Nguyen, Angus C. Nairn, Audrey N. Chang
Summary: In this study, the researchers identified the main isoform of PP1c beta expressed in cardiac myocytes and investigated its role in maintaining RLC phosphorylation in vivo. They found that cardiac muscle pathogenesis in PP1c beta knockout animals involves upregulation of total PP1c alpha in myocytes and non-muscle cells. Additionally, they discovered that phosphorylated myofibrillar cardiac myosin is dephosphorylated by both myosin-targeted and soluble MYPT-independent PP1c beta activities. These findings enhance our understanding of cardiac-MLCP in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Kohji Yamada, Tsunekazu Oikawa, Ryusuke Kizawa, Saya Motohashi, Saishu Yoshida, Tomotaka Kumamoto, Chisato Saeki, Chika Nakagawa, Yuya Shimoyama, Katsuhiko Aoki, Toshiaki Tachibana, Masayuki Saruta, Masaya Ono, Kiyotsugu Yoshida
Summary: The secreted PKC delta from liver cancer cells acts as a humoral growth factor that enhances cell growth by activating proliferative signaling molecules. Inactivation of cytosolic PKC delta is necessary for its secretion, and it is anchored on the cell surface of liver cancer cells via association with glypican 3.
Article
Multidisciplinary Sciences
Ahmed Wagdi, Daniela Malan, Udhayabhaskar Sathyanarayanan, Janosch S. Beauchamp, Markus Vogt, David Zipf, Thomas Beiert, Berivan Mansuroglu, Vanessa Dusend, Mark Meininghaus, Linn Schneider, Bernd Kalthof, J. Simon Wiegert, Gabriele M. Konig, Evi Kostenis, Robert Patejdl, Philipp Sasse, Tobias Bruegmann
Summary: This study presents a new optogenetic tool, human Neuropsin (hOPN5), which allows for specific and repetitive manipulation of G(q) signaling with high spatio-temporal resolution. The researchers characterized the properties and G protein specificity of hOPN5 using UV light-induced IP3 generation, Ca2+ transients, and inhibition of G(IRK) channel activity. They demonstrated that light could increase the beating rate of adult hearts and induce contractions in the small intestine, both of which were dependent on G(q) signaling. Additionally, the authors showed that all-optical high-throughput screening using hOPN5 was more specific and sensitive compared to conventional pharmacological screening.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Monica Dalva, Ida K. Lavik, Khadija El Jellas, Anny Gravdal, Aurelia Lugea, Stephen J. Pandol, Pal R. Njolstad, Richard T. Waldron, Karianne Fjeld, Bente B. Johansson, Anders Molven
Article
Cell Biology
Gaston Amable, Eduardo Martinez-Leon, Maria Elisa Picco, Sergio Nemirovsky, Enrique Rozengurt, Osvaldo Rey
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Cell Biology
Cintia E. Citterio, Sofia Siffo, Christian M. Moya, Mauricio Gomes Pio, Maricel F. Molina, Karen G. Scheps, Osvaldo A. Rey, Peter Arvan, Carina M. Rivolta, Hector M. Targovnik
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2020)
Article
Oncology
Mingtian Che, Soo-Mi Kweon, Jia-Ling Teo, Yate-Ching Yuan, Laleh G. Melstrom, Richard T. Waldron, Aurelia Lugea, Raul A. Urrutia, Stephen J. Pandol, Keane K. Y. Lai
Review
Gastroenterology & Hepatology
Zobeida Cruz-Monserrate, Kristyn Gumpper, Valentina Pita, Phil A. Hart, Christopher Forsmark, David C. Whitcomb, Dhiraj Yadav, Richard T. Waldron, Stephen Pandol, Hanno Steen, Vincent Anani, Natasha Kanwar, Santhi Swaroop Vege, Savi Appana, Liang Li, Jose Serrano, Jo Ann S. Rinaudo, Mark Topazian, Darwin L. Conwell
Summary: Chronic pancreatitis lacks diagnostic or prognostic biomarkers, but potential candidates have been identified. Five biomarkers showed high effectiveness in distinguishing CP cases, while 30 had moderate effectiveness. Variability in study design, criteria, and sample handling was noted, suggesting the need for standardized approaches in future research.
Article
Agriculture, Dairy & Animal Science
Brenda Florencia Gimeno, Maria Victoria Bariani, Lucia Laiz-Quiroga, Eduardo Martinez-Leon, Micaela Von-Meyeren, Osvaldo Rey, Adrian Angel Mutto, Claudia Elena Osycka-Salut
Summary: This study improved the yield of fertilization-capable sperm from equine frozen sperm by optimizing manipulation conditions and establishing a primary culture model of oviductal cells. The in vitro model demonstrated the potential fertilizing capacity of sperm released from oviductal cells, suggesting advancements in handling and selection of cryopreserved sperm for improved efficiencies in assisted reproductive techniques.
Letter
Gastroenterology & Hepatology
Marc T. Goodman, Simon K. Lo, Dhiraj Yadav, Bechien U. Wu, Laith H. Jamil, Karl K. Kwok, Georgios I. Papachristou, Elham Afghani, Yunhee Choi-Kuaea, Richard T. Waldron, Christina Lombardi, Christie Y. Jeon, Irene B. Helenowski, Ellen Richmond, Kelly Benante, Aida Habtezion, Tia Schering, Seema A. Khan, Luz M. Rodriguez, Stephen J. Pandol
Article
Biochemistry & Molecular Biology
Cheng Hu, Liyuan Yin, Zhiyao Chen, Richard T. Waldron, Aurelia Lugea, Yiyun Lin, Xiaoqian Zhai, Li Wen, Yuan-Ping Han, Stephen J. Pandol, Lihui Deng, Qing Xia
Summary: Chronic pancreatitis (CP) is closely related to irreversible fibro-inflammatory changes induced by pancreatic stellate cells (PSC), which have unique transcriptional profiles. Some genes in PSC may serve as biomarkers for CP.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Meeting Abstract
Gastroenterology & Hepatology
C. Hu, L. Yin, Z. Chen, R. T. Waldron, A. Lugea, Y. Lin, X. Zhai, Y. Han, S. J. Pandol, L. Deng, Q. Xia
Meeting Abstract
Gastroenterology & Hepatology
C. Hu, R. T. Waldron, C. Quiros, Q. Xia, B. Ji, S. J. Pandol, A. Lugea
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, S. Parker, J. Van Eyk, G. I. Papachristou, S. J. Pandol
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, L. Huo, P. Yue, J. Turkson, S. J. Pandol
Meeting Abstract
Gastroenterology & Hepatology
J. Wang, J. Wan, O. Wang, M. Jaggarapu, A. Haddock, R. T. Waldron, A. Lugea, S. J. Pandol, Y. Wang, Y. Bi, B. Ji
Meeting Abstract
Gastroenterology & Hepatology
R. T. Waldron, A. Lugea, K. Stello, G. I. Papachristou, S. J. Pandol
Review
Physiology
Qiong Wei, Liang Qi, Hao Lin, Dechen Liu, Xiangyun Zhu, Yu Dai, Richard T. Waldron, Aurelia Lugea, Mark O. Goodarzi, Stephen J. Pandol, Ling Li
FRONTIERS IN PHYSIOLOGY
(2020)