Article
Multidisciplinary Sciences
Andrea Piserchio, Kimberly J. Long, Luke S. Browning, Amanda L. Bohanon, Eta A. Isiorho, Kevin N. Dalby, Ranajeet Ghose
Summary: Protein translation, a high-energy process, requires robust regulation under energy-deprived conditions. The eEF-2K plays a crucial role in this regulation by suppressing translational elongation through phosphorylation of eEF-2. It has been suggested that eEF-2K's response to energy levels is mediated by AMPK and the mTOR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Timothy W. Church, Parul Tewatia, Saad Hannan, Joao Antunes, Olivia Eriksson, Trevor G. Smart, Jeanette Hellgren Kotaleski, Matthew G. Gold
Summary: The study found that AKAP79 enhances calcineurin activity, which leads to the suppression of PKA without altering cAMP levels. This non-canonical mode of PKA regulation may play a role in many other cellular processes.
Article
Biochemistry & Molecular Biology
Veronica A. Cochrane, Zhongying Yang, Mark L. Dell'Acqua, Show-Ling Shyng
Summary: The study revealed that leptin regulates the trafficking of K-ATP channels in pancreatic beta-cells by affecting PKA activity. Increased PKA activity depends on NMDA receptors, CaMKK beta, and AMPK, and is anchored to AKAP79/150 at the cell membrane. disrupting AKAP79/150 coordination may provide a potential target for restoring insulin secretion in beta-cells with defective leptin signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Anastasiia Bohush, Wieslawa Lesniak, Serge Weis, Anna Filipek
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the formation of Lewy bodies and loss of dopaminergic neurons. Changes in intracellular Ca2+ levels can have serious consequences on cells, with calmodulin playing a crucial role in regulating neuronal plasticity. Therefore, alterations in the activity of calmodulin and its binding proteins may be linked to the development and progression of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Takahiro Eguchi, Yuji Yamanashi
Summary: A reduction in muscle mass and strength is commonly observed in various conditions. Recent studies have identified CaMKII beta as a potential molecular mechanism that controls muscle mass and strength. By injecting different recombinant adeno-associated virus (AAV) vectors into mouse muscles, researchers found that the exogenous expression of CaMKII beta significantly increased or decreased muscle mass and strength. These findings suggest that CaMKII beta could be a novel therapeutic target for enhancing muscle mass and strength.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Andrew C. Hedman, Zhigang Li, Laetitia Gorisse, Swetha Parvathaneni, Chase J. Morgan, David B. Sacks
Summary: AMP-activated protein kinase (AMPK) is crucial in maintaining cellular energy homeostasis through regulating catabolic and anabolic pathways, with IQGAP1 identified as a novel regulator of AMPK signaling. Lack of IQGAP1 leads to impaired AMPK activation and metabolic dysregulation, highlighting the importance of the functional interplay between IQGAP1 and AMPK.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Forestry
Wei Tang, Anna Y. Tang
Summary: Transcription factors, such as ZAT6, were introduced into rice, cotton, and slash pine cells to enhance resistance against pathogenic bacteria, resulting in increased cell viability and growth rate. The study showed that ZAT6 enhances plant cell resistance toPseudomonas syringae by modulating the transcription of CPK and MAPK genes as well as oxidase activity.
JOURNAL OF FORESTRY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yagmur Azbazdar, Nydia Tejeda-Munoz, Julia C. Monka, Alex Dayrit, Grace Binder, Gunes Ozhan, Edward M. De Robertis
Summary: Activation of Wnt signaling leads to macropinocytosis, which promotes tumor progression. Addition of the second messenger lipid sn-1,2 DAG induces macropinocytosis rapidly. Moreover, DAG addition enhances the effects of Wnt3a recombinant protein and GSK3 inhibitor LiCl in luciferase transcriptional reporter assays. In colorectal carcinoma cells with constitutive Wnt signaling, DAG addition increases nuclear beta-catenin levels, which can be partially inhibited by a macropinocytosis inhibitor. DAG also expands multivesicular bodies marked by CD63 protein. In vivo, microinjection of DAG induces Wnt-like twinned body axes in Xenopus embryos when co-injected with small amounts of LiCl.
Article
Cardiac & Cardiovascular Systems
Ji Cao, Jingjing Zhang, Jianan Qian, Xue Wang, Wei Zhang, Xiangfan Chen
Summary: This study investigated the effect of RIPK3-regulated CaMKII on necroptosis in heart failure and its mechanism. The results showed that depletion of RIPK3 alleviated cardiac dysfunction, CaMKII activation, and necroptosis. These findings provide valuable insights into the pathogenesis and treatment strategy of heart failure.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sarah G. Cook, Nicole L. Rumian, K. Ulrich Bayer
Summary: CaMKII T286 autophosphorylation is required for both forms of hippocampal LTD, but with differential requirements for heterosynaptic communication of excitatory signals to inhibitory synapses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Iskander Madhi, Ji-Hee Kim, Ji Eun Shin, Younghee Kim
Summary: Ginsenoside Re (G-Re) inhibits the production of inflammatory mediators in microglial cells and protects hippocampal cells from inflammatory and neurotoxic factors. This suggests G-Re may be a potential treatment option for neuroinflammatory disorders and various neurodegenerative diseases.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Neurosciences
James P. Mackay, Amy I. Smith-Dijak, Ellen T. Koch, Peng Zhang, Evan Fung, Wissam B. Nassrallah, Caodu Buren, Mandi Schmidt, Michael R. Hayden, Lynn A. Raymond
Summary: Miniature neurotransmission is increased in the Huntington disease (HD) model, associated with abnormal endoplasmic reticulum (ER) calcium handling. These abnormalities influence neurotransmission indirectly, without direct ER calcium release into the cytoplasm. However, in cortical cultures and brain slices, there are no significant differences in calcium release between the HD-model neurons and wild-type cells.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Akhmed Aslam, Jawwad Ahmad, Mohammed A. Baghdadi, Shakir Idris, Riyad Almaimani, Aiman Alsaegh, Mai Alhadrami, Bassem Refaat
Summary: This study found that vitamin D is superior to Paricalcitol in potentiating 5-FU cytotoxicity against colorectal cancer, possibly by upregulating several Ca2+-related molecules involved in tumor suppression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Fu-Sheng Liu, Chang Jiang, Zheng Li, Xiao-Bin Wang, Jing Li, Bing Wang, Guo-Hua Lv, Fu-Bing Liu
Summary: This study establishes an in vitro model to explore the relationship among spinal cord injury, intracellular calcium ions, and autophagy. Mechanical injury is found to promote the increase of intracellular calcium concentration, which activates autophagy through the CaMKK beta/AMPK/mTOR pathway and also triggers apoptosis. This study provides new insights into the mechanisms of spinal cord injury and opens up new possibilities for its treatment.
NEUROCHEMICAL RESEARCH
(2023)