Article
Biology
Yilan Zhen, Carlie L. Cullen, Raphael Ricci, Benjamin S. Summers, Sakina Rehman, Zubair M. Ahmed, Antoinette Y. Foster, Ben Emery, Robert Gasperini, Kaylene M. Young
Summary: Protocadherin 15 regulates ERK1/2 phosphorylation to reduce proliferation and promotes lamellipodial and filopodial dynamics in oligodendrocyte progenitor cells by controlling Cdc42-Arp2/3 activity. It plays a critical role in the function of these cells in the healthy central nervous system and may have implications in glioma progression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Angela Ballesteros, Manoj Yadav, Runjia Cui, Kiyoto Kurima, Bechara Kachar
Summary: This study reveals the selective transport of isoforms of protocadherin 15 (PCDH15) by myosins using a heterologous expression system. The results show that MYO7A transports the CD3 isoform, while MYO3A and MYO3B transport the CD2 isoform. In addition, MYO15A has an insignificant role in the transport of PCDH15, and none of the tested myosins transport PCDH15-CD1. The study highlights the importance and complexity of mechanoelectrical transduction (MET) and actin regulation in the formation and maturation of the stereocilia bundle.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Eric M. Mulhall, Andrew Ward, Darren Yang, Mounir A. Koussa, David P. Corey, Wesley P. Wong
Summary: Researchers characterized the strength of the tip-link connection at single-molecule resolution and found that a single tip-link bond is more mechanically stable relative to classic cadherins, and that the double stranded tip-link connection is stabilized by single strand rebinding facilitated by strong cis-dimerization domains.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xiankui Cui, Shuwei Wang, Yaohui Huang, Xuening Ding, Zirong Wang, Lidan Zheng, Yujing Bi, Fanghui Ge, Lei Zhu, Ming Yuan, Shaul Yalovsky, Ying Fu
Summary: This study uncovers a direct link between an ROP GTPase and vesicular trafficking and a new mechanism for the control of apical exocytosis, whereby ROP GTPase signaling spatially regulates SNARE complex assembly and the polar distribution of a Q-SNARE.
Article
Biochemistry & Molecular Biology
Siroshini K. Thiagarajan, Siew Ying Mok, Satoshi Ogawa, Ishwar S. Parhar, Pek Yee Tang
Summary: This study used zebrafish larvae as a model to investigate the effects of dopamine receptor antagonists and morphine on social behavior and locomotion. The levels of dopamine and the number of dopaminergic neurons were measured, and gene expression analysis revealed changes associated with schizophrenia. Termination of morphine use also led to changes in gene expression. The integrated data suggest that the dopamine system plays a key role in the schizophrenia-like symptoms and opioid dependence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ghassan Bkaily, Danielle Jacques
Summary: Calcium is a positively charged ionic species that regulates various cell functions and acts as a second messenger. Abnormal calcium homeostasis can lead to diseases such as cardiovascular disorders, skeletal disorders, immune disorders, secretion disorders, and cancer. Therefore, pharmacological control of calcium transport is important in treating these conditions, particularly in the cardiovascular system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Multidisciplinary
Xu Xu, Ma Wen-Kai, Yao Wen-Juan
Summary: This study investigates the role of stereocilia motion and tip-link tension in the active acoustic amplification mechanism of the cochlea. By considering the elastic properties and shaft bending of the stereocilia, an analytical solution for their motion is derived. It is found that noise exposure affects the tip-link tension, resulting in changes in the cochlea's ability to perceive sound and frequency selectivity. The presence of lymphatic fluid plays a crucial role in determining the frequency characteristics of the hair bundle.
ACTA PHYSICA SINICA
(2022)
Article
Plant Sciences
Hong Thi Lam Phan, Yu Ran Nam, Hyun Jong Kim, Joo Han Woo, Wan NamKung, Joo Hyun Nam, Woo Kyung Kim
Summary: Magnolol has potential as a promising therapeutic agent for the treatment and prevention of allergic rhinitis (AR), possibly through the modulation of ANO1 and ORAI1 channels expressed in nasal epithelial cells and T-lymphocytes.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hong T. L. Phan, Hyun J. Kim, Sungwoo Jo, Woo K. Kim, Wan Namkung, Joo H. Nam
Summary: Calcium signaling in T-lymphocytes is regulated by ORAI1 and potassium channels, and Licochalcone A effectively inhibits these channels to suppress T-cell activation and proliferation, making it a potential treatment for inflammation-related immune diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Camilla F. F. Silva, Clebio Soares Nascimento Jr, Keyller Bastos Borges
Summary: A HPLC-UV method using restricted access polypyrrole as an adsorbent in pipette-tip solid-phase extraction (PT-SPE) has been developed to determine nimodipine and nicardipine in breast milk. The method showed good recoveries, linearity, and correlation coefficients, and has been successfully applied in analyses of breast milk from volunteers.
ANALYTICAL METHODS
(2023)
Article
Neurosciences
David Oestreicher, Maria Magdalena Picher, Vladan Rankovic, Tobias Moser, Tina Pangrsic
Summary: Clinical management of auditory synaptopathies and other genetic hearing disorders is currently limited to the use of hearing aids or cochlear implants, but future gene therapy holds promise for restoring hearing in certain forms of monogenic hearing impairment. Preclinical studies using gene therapy in Cabp2(-/-) mice have shown potential for treating DFNB93, demonstrating high transduction efficiency and restoration of inner hair cell function.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Plant Sciences
Feifei Huang, Feihua Wu, Min Yu, Sergey Shabala
Summary: Programmed cell death is a genetically controlled process that eliminates unnecessary or damaged cells. Recent studies have identified nucleotide-binding leucine-rich repeat proteins as a novel class of Ca2+-permeable channels which play a role in plant immune responses. These channels possess a unique structure and can also permeate other cations, making them ideal candidates for mediating cell signaling and adaptive responses to various stress factors, including pathogens and abiotic stresses.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Neurosciences
Jing-Yi Jeng, Csaba Harasztosi, Adam J. Carlton, Laura F. Corns, Philine Marchetta, Stuart L. Johnson, Richard J. Goodyear, Kevin P. Legan, Lukas Ruettiger, Guy P. Richardson, Walter Marcotti
Summary: The study aimed to investigate the effect of detachment of the tectorial membrane from the organ of Corti in Tecta/Tectb(-/-) mice on the biophysical properties of cochlear outer hair cells (OHCs). It was found that the Tecta/Tectb(-/-) mice have elevated hearing thresholds and impaired adaptation of MET-dependent otoacoustic emissions. The results suggest that the tectorial membrane may play a role in regulating extracellular Ca2+ levels around the OHC MET channels during sound stimulation.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Pharmacology & Pharmacy
Patricia Sinclair, Nadine Kabbani
Summary: This article reviews the evidence of ionotropic and metabotropic signaling responses of α7 nAChRs in various cell types, and explores the importance of these signaling responses in regulating cytoskeletal motility and cell growth.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Shai Abehsera, Shmuel Bentov, Xuguang Li, Simy Weil, Rivka Manor, Shahar Sagi, Shihao Li, Fuhua Li, Isam Khalaila, Eliahu D. Aflalo, Amir Sagi
Summary: In this study, the researchers suggest that bicarbonate ion transport mechanisms for mineralization in crustaceans may involve the SLC4 family of transporters, and that these proteins play a key role in the tight coupling between molt cycle events and mineral deposition. This discovery of putative bicarbonate transporters in a pancrustacean with functional genomic evidence from genes encoding the SLC4 family-mostly known for their role in pH control-is discussed in the context of the evolution of calcium carbonate biomineralization.
SCIENTIFIC REPORTS
(2021)