Article
Plant Sciences
Emilija Dukic, Kim A. van Maldegem, Kashif Mohd Shaikh, Kento Fukuda, Mats Toepel, Katalin Solymosi, Jonna Hellsten, Thomas Hesselhoj Hansen, Soren Husted, John Higgins, Satoshi Sano, Sumio Ishijima, Cornelia Spetea
Summary: Magnesium (Mg2+) is crucial for photosynthesis in plants and algae, and its concentration in chloroplasts is tightly regulated by transport proteins. This study identifies three different transporters and investigates their roles in maintaining chloroplast Mg2+ homeostasis and regulating photosynthesis in Arabidopsis thaliana and Chlamydomonas reinhardtii. The findings reveal essential but differential roles of these transporters in chloroplast Mg2+ homeostasis and highlight their conservation across plant species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Wanzhu Li, Meiling Yang, Baoli Wang, Cong-Qiang Liu
Summary: Phytoplankton carbon and nitrogen stoichiometric homeostasis is important in aquatic ecosystems, but the regulatory strategy for its plasticity is unclear. This study investigates the variations in phytoplankton C:N ratios and the regulatory strategy for cellular C-N stoichiometric homeostasis under different C and N availability. The results show that CO2 and nitrate limitations have different effects on phytoplankton C:N ratios, and different gene-regulated intensities are involved in the regulation. This study deepens the understanding of C-N stoichiometric homeostasis in freshwater phytoplankton.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Yimei Xi, Bolei Han, Fantao Kong, Tingting You, Ran Bi, Xiangfeng Zeng, Shaofeng Wang, Yongfeng Jia
Summary: A novel phosphate transporter DsPht1 was found to be highly upregulated in Dunaliella salina after arsenic exposure. Overexpressing DsPht1 in Chlamydomonas reinhardtii increased arsenic uptake and transformation, making it promising for effective phytoremediation of arsenic-contaminated water.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Thi Thu Hoai Ho, Chris Schwier, Tamar Elman, Vera Fleuter, Karen Zinzius, Martin Scholz, Iftach Yacoby, Felix Buchert, Michael Hippler
Summary: Linear electron flow (LEF) and cyclic electron flow (CEF) compete for light-driven electrons, which can also be used for hydrogen production in anoxic conditions. The partitioning of electrons is regulated by PROTON-GRADIENT REGULATION5 (PGR5) and PSI remodeling processes. The plasticity of photosystem I-associated light-harvesting proteins LHCA2 and LHCA9 affects photosynthetic electron transfer and hydrogen production.
Article
Biotechnology & Applied Microbiology
Guanqin Huang, Di Zhao, Chengxiang Lan, Bowen Wu, Xiaolian Li, Sulin Lou, Yihong Zheng, Ying Huang, Zhangli Hu, Bin Jia
Summary: Genetic engineering techniques offer a key method for enhancing the biomass of microalgae by altering their nutritional patterns. In this study, the expression of the glucose transporter gene was investigated, and it was found that engineered algae showed better growth than control algae under heterotrophic and mixotrophic conditions. However, the growth rates and biomass of the engineered algae were relatively low, possibly due to the inability of glucose to enter the chloroplasts.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Multidisciplinary Sciences
Lital Davidi, Sean D. Gallaher, Eyal Ben-David, Samuel O. Purvine, Thomas L. Fillmore, Carrie D. Nicora, Rory J. Craig, Stefan Schmollinger, Sanja Roje, Crysten E. Blaby-Haas, Robert P. Auber, Jennifer H. Wisecaver, Sabeeha S. Merchant
Summary: This article investigates the adaptation mechanisms of two marine algae species under iron-deficient conditions. The study reveals multiple iron uptake routes in these species and their ability to adjust protein expression and structure to cope with iron deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Sara M. Esteves, Alice Jadoul, Fabrizio Iacono, Marie Schloesser, Bernard Bosman, Monique Carnol, Tom Druet, Pierre Cardol, Marc Hanikenne
Summary: This study reveals extensive genetic variation in Chlamydomonas reinhardtii and describes how this variation influences the response to nutrient deficiency, with a focus on differences between natural and laboratory strains.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Eva YuHua Kuo, Tse-Min Lee
Summary: The acclimation mechanism of Chlamydomonas reinhardti to nitric oxide involves a temporally orchestrated implementation of metabolic processes, including modulation of NADPH oxidase and ROS signaling pathways, triggering of NO scavenging elements, prevention of photo-oxidative risk through photosynthesis inhibition, adaptation to nitrogen and sulfur shortage, and dynamic regulation of protein homeostasis. The gene encoding NADPH oxidase, RBOL2, shows a transient increase after NO challenge, suggesting its involvement in Chlamydomonas response to NO stress.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
David A. Doetsch, Salim Ansari, Ole Jensen, Lukas Gebauer, Christof Duecker, Jurgen Brockmoeller, Alexandra Sachkova
Summary: This study characterized the substrate spectrum of a proton-organic cation (H + OC) antiporter, which is responsible for transporting organic cations through membranes. Using hCMEC/D3 cells as a model, the researchers examined the uptake of 72 drugs and identified 37 of them as good substrates of the H + OC antiporter. Further analysis showed that the substrates had specific characteristics, such as being more hydrophobic and having a lower topological polar surface area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Alexandra Sachkova, Ole Jensen, Christof Duecker, Salim Ansari, Juergen Brockmoeller
Summary: About 30% of small molecular drugs are organic cations that require membrane transporters to pass through biological membranes. The proton-organic cation antiporter, known as H+ OC antiporter, may have a significant role in the transport of organic cations through the blood-brain barrier. However, the gene coding for this antiporter has not yet been identified. This review summarizes the functional characteristics, expression, and substrate spectrum of the H+ OC antiporter.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Alexandra Sachkova, David Alexander Doetsch, Ole Jensen, Juergen Brockmoeller, Salim Ansari
Summary: The study found that all the psychostimulants studied, except for mescaline, were substrates of the H+/OC antiporter, emphasizing the need for a better characterization of this transport protein.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yusuke Kuwana, Yuya Ashizawa, Miki Ajima, Takuya Nomura, Mayu Kakeno, Shunya Hirai, Takashi Miura
Summary: This study investigated the interactions between endomorphin-1 and copper ions. Endomorphin-1 binds to copper ions through the pi-electrons of its aromatic side chains, inhibiting copper-catalyzed oxidation/reduction reactions. Due to its ability to bind both Cu(II) and Cu(I), endomorphin-1 may have the potential to suppress copper-mediated oxidative stress in the brain.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Plant Sciences
Elena Kreis, Justus Niemeyer, Marco Merz, David Scheuring, Michael Schroda
Summary: In plant cells, the CLP/HSP100 chaperone family member HSP101 plays a crucial role in resolving heat-induced protein aggregates. However, the role of the chloroplast family member CLPB3 in this process remains unclear. In this study, two clpb3 mutants in Chlamydomonas reinhardtii were characterized, revealing that chloroplast CLPB3 is required for resolving heat-induced protein aggregates and conferring thermotolerance. CLPB3 was found to interact with HSP22E/F near the thylakoid membranes, suggesting a possible role in disentangling protein aggregates from the thylakoid membrane system.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Jianfang Li, Like Shen, Xiuli Han, Gefeng He, Wenxia Fan, Yu Li, Shiping Yang, Ziding Zhang, Yongqing Yang, Weiwei Jin, Yi Wang, Wenhua Zhang, Yan Guo
Summary: The maintenance of Na+/K+ homeostasis is crucial for salt tolerance in plant cells. In this study, it is shown that phosphatidic acid (PA) binds to SOS2, a core member of the SOS pathway, under salt stress, enhancing its activity and plasma membrane localization. PA also promotes the phosphorylation of SCaBP8 by SOS2, which attenuates the inhibition of AKT1, an inward-rectifying K+ channel. These results suggest that PA regulates the SOS pathway and AKT1 activity under salt stress, promoting Na+ efflux and K+ influx to maintain Na+/K+ homeostasis.
Article
Plant Sciences
Muhammad Farooq, Jae-Ryoung Park, Yoon-Hee Jang, Eun-Gyeong Kim, Kyung-Min Kim
Summary: This study demonstrates that under salt stress, the Nagdong cultivar has better salt tolerance than the Cheongcheong cultivar, as evidenced by higher germination rate, plant vigor, Na+/K+ ratio, and expression of genes related to salt tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Environmental Sciences
Andrew P. Dean, Antoni Hartley, Owen A. McIntosh, Alyssa Smith, Helen K. Feord, Nicolas H. Holmberg, Thomas King, Ellen Yardley, Keith N. White, Jon K. Pittman
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Biotechnology & Applied Microbiology
Elia D. Charles, Howbeer Muhamadali, Royston Goodacre, Jon K. Pittman
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2019)
Article
Environmental Sciences
Oscar E. Aguinaga, James F. T. Wakelin, Keith N. White, Andrew P. Dean, Jon K. Pittman
Article
Environmental Sciences
Richard Chalkley, Frederick Child, Khalil Al-Thaqafi, Andrew P. Dean, Keith N. White, Jon K. Pittman
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2019)
Article
Biotechnology & Applied Microbiology
Gonzalo M. Figueroa-Torres, Wan M. Asyraf Wan Mahmood, Jon K. Pittman, Constantinos Theodoropoulos
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Microbiology
Lynn Foster, Howbeer Muhamadali, Christopher Boothman, David Sigee, Jon K. Pittman, Royston Goodacre, Katherine Morris, Jonathan R. Lloyd
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Agricultural Engineering
Mohammad Khalil Monjed, Geoffrey D. Robson, Jon K. Pittman
BIOMASS & BIOENERGY
(2020)
Article
Environmental Sciences
Lynn Foster, Christopher Boothman, Sharon Ruiz-Lopez, Genevieve Boshoff, Peter Jenkinson, David Sigee, Jon K. Pittman, Katherine Morris, Jonathan R. Lloyd
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Biochemistry & Molecular Biology
Snezana Vojvodic, Marina Stanic, Bernd Zechmann, Tanja Ducic, Milan Zizic, Milena Dimitrijevic, Jelena Danilovic Lukovic, Milica R. Milenkovic, Jon K. Pittman, Ivan Spasojevic
BIOCHEMICAL JOURNAL
(2020)
Article
Environmental Sciences
Oscar E. Aguinaga, Keith N. White, Andrew P. Dean, Jon K. Pittman
Summary: This study found that the utilization of organic carbon by microorganisms is more important for efficient AMD treatment than the availability of oxygen. Combining oxygen with organic carbon did not inhibit improvements to water quality.
ENVIRONMENTAL POLLUTION
(2021)
Article
Ecology
James M. Dinsley, Helena S. Davies, Miguel A. Gomez-Gonzalez, Clare H. Robinson, Jon K. Pittman
Summary: Synchrotron radiation techniques are valuable analytical methods that have been widely used in physical, geological, and biochemical research. In recent years, they have become more accessible for ecological and evolutionary research at the micro- or nanoscale. However, there are still gaps in the imaging of certain elements and the study of specific species, and sample preparation is crucial for obtaining high-quality data. These techniques have great potential in agri-ecology, paleoclimatology, and comparative and functional morphology studies.
Article
Chemistry, Physical
Gonzalo M. Figueroa-Torres, Jon K. Pittman, Constantinos Theodoropoulos
Summary: Microalgal biomass is a promising source for green energy generation, but conventional cultivation methods have low productivity. This study presents a highly productive fed-batch cultivation strategy using intermittent pulses of organic carbon to promote microalgal growth and increase starch and lipid formation.
SUSTAINABLE ENERGY & FUELS
(2022)
Article
Biodiversity Conservation
Jeanette Rosas-Moreno, Jon K. Pittman, Clare H. Robinson
Summary: Industrial activities have caused pollution by radionuclides and heavy metals, which poses a concern for the ecosystem. This study investigates how arbuscular mycorrhizal fungi contribute to the mobilisation of non-essential elements in contaminated environments, providing insights for the management and remediation of such sites.
PLANTS PEOPLE PLANET
(2021)
Article
Biochemistry & Molecular Biology
Aniefon Ibuot, Andrew P. Dean, Jon K. Pittman
Proceedings Paper
Computer Science, Interdisciplinary Applications
Alessandro Usai, Jon Pittman, Constantinos Theodoropoulos
29TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING, PT B
(2019)