Article
Biochemistry & Molecular Biology
Qing Meng, Qiuqiang Gao, Shebli Mehrazarin, Kamonchanok Tangwanichgapong, Yu Wang, Yiming Huang, Yutong Pan, Samuel Robinson, Ziwen Liu, Amirali Zangiabadi, Renate Lux, Panos N. Papapanou, X. Edward Guo, Harris Wang, Luke E. Berchowitz, Yiping W. Han
Summary: Fusobacterium nucleatum secretes an adhesin with amyloid properties to enhance virulence, inducing periodontal bone loss and promoting colorectal cancer progression in mice. Virulence can be attenuated by amyloid-binding compounds.
Article
Immunology
Dong-Hao Li, Zheng-Peng Li, Yan Zhang, Guan-Zhou Zhou, Rong-Rong Ren, Hui-Jun Zhao, Na-Na Zhang, Jian-Feng Li, Li-Hua Peng, Yun-Sheng Yang
Summary: The presence of Fusobacterium nucleatum (F.nucleatum) and its invasive virulence gene fadA increased in patients with ulcerative colitis, especially in those with severe colitis and pancolitis. Strains of F.nucleatum carrying the virulence gene fadA are suggested to play a role in the pathogenesis of UC.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Orazio Palmieri, Stefano Castellana, Anna Latiano, Tiziana Latiano, Annamaria Gentile, Anna Panza, Marianna Nardella, Davide Ciardiello, Tiziana Pia Latiano, Giuseppe Corritore, Tommaso Mazza, Francesco Perri, Giuseppe Biscaglia
Summary: An increasing amount of evidence suggests the important role of gut microbiota in the development of colorectal cancer. This study analyzed the microbial communities in normal and neoplastic colonic mucosa using NGS and metagenomics analysis tools. The findings revealed weak differences in microbial diversity between CRC and control groups, with an increasing trend of Rikenellaceae, Pseudomonas, and Fusobacterium in CRC. Fusobacterium nucleatum was found to be significantly enriched in all tissues of subjects with synchronous colorectal neoplasia.
Article
Dentistry, Oral Surgery & Medicine
Zhiying Zhang, Sai Liu, Shuwei Zhang, Yuchao Li, Xiaoting Shi, Dongjuan Liu, Yaping Pan
Summary: The study revealed that proteases in P. gingivalis outer membrane vesicles inhibited the invasion of F. nucleatum into oral epithelial cells by downregulating FadA and FomA. F. nucleatum, on the other hand, promoted the invasion of P. gingivalis. Proteases inside P. gingivalis OMVs did not affect the morphology and proliferation of F. nucleatum, but decreased its auto-aggregation.
JOURNAL OF PERIODONTOLOGY
(2022)
Review
Medicine, Research & Experimental
Slater L. Clay, Diogo Fonseca-Pereira, Wendy S. Garrett
Summary: The microbiota plays an important role in the development of colorectal cancer (CRC). Identifying specific microbial features that impact CRC initiation and progression is crucial. Bacterial factors, such as toxins, contribute to colorectal carcinogenesis. However, further research is needed to understand the host determinants of a toxin's cancer-promoting effects. For bacteria associated with CRC but lacking toxins, defining cancer-relevant virulence factors is challenging. Additionally, gut microbes can have beneficial effects on CRC antitumor immunity through the production of certain metabolites.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Tian Zhao, Jiaqi Chen, Shuai Liu, Jie Yang, Juan Wu, Leiying Miao, Weibin Sun
Summary: Periodontitis is a chronic infectious disease that can lead to various dental problems and even tooth loss. The dental plaque biofilm is a crucial factor in the development of periodontitis. Fusobacterium nucleatum plays an important role in the structure and ecology of dental plaque biofilms. Understanding the molecular mechanism of F. nucleatum during biofilm development is essential for controlling periodontitis. This study used RNA-sequencing and quantitative reverse transcriptase PCR (RT-qPCR) to analyze the gene expression profiles of F. nucleatum in the planktonic and biofilm phases. The results revealed significant differences in gene expression between the two states, with several genes related to metabolism, cell growth, division, oxidative stress, virulence, and oral malodor being differentially expressed. Additionally, the study identified lysine as a potential antibacterial and antibiofilm agent against F. nucleatum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Shanru He, Yuanyuan Chen, Lulu Wang, Xue Bai, Tingting Bu, Jie Zhang, Ming Lu, Nam-Chul Ha, Chunshan Quan, Ki Hyun Nam, Yongbin Xu
Summary: YggS is a protein that binds to PLP and regulates its balance in bacteria and humans. This study analyzed the function and structure of YggS from Fusobacterium nucleatum and found that its overall structure and PLP-binding site are highly conserved, playing a key role in PLP binding.
Article
Microbiology
Hyeok Ahn, Kyungchan Min, Eulgi Lee, Hyun Kim, Sujeong Kim, Yunjae Kim, Gihyeon Kim, Beomki Cho, Chanyeong Jeong, Yeongmin Kim, Hansoo Park
Summary: Remarkable progress has been made in the past two decades in identifying microbiomes that affect the human body, especially in relation to gastrointestinal (GI) tract cancer. This study focused on Korean patients with GI tract cancer to determine if there is a common microbiome among these cancers and how it impacts the disease. The findings revealed that Fusobacterium nucleatum, a Gram-negative obligate anaerobe, is enriched in all cancer tissues and promotes colorectal cancer through the upregulation of the G2M checkpoint pathway. These results suggest that F. nucleatum could be a potential therapeutic target for Korean patients with GI tract cancer.
Article
Biology
L. Berbert, A. Santos, D. O. Magro, D. Guadagnini, H. B. Assalin, L. H. Lourenco, C. A. R. Martinez, M. J. A. Saad, C. S. R. Coy
Summary: This study investigated the composition of gut microbiota in Brazilian colorectal adenocarcinoma (CRC) patients compared with a control population. The results showed that CRC patients had increased alpha diversity, higher percentage of Prevotella genus, and decreased proportion of Megamonas and Ruminococcus. Faecalibacterium prausnitzii was associated with a better prognosis in early stages of CRC, while Fusobacterium nucleatum was identified as an important marker of colorectal carcinogenesis and tumor aggressiveness.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Xiaomei Ma, Tianyong Sun, Jiannan Zhou, Mengfan Zhi, Song Shen, Yushang Wang, Xiufeng Gu, Zixuan Li, Haiting Gao, Pingping Wang, Qiang Feng
Summary: This study analyzed the genomic characteristics and pathogenic effectors of F. nucleatum strains, revealing similarities and differences in functional gene compositions and virulence factors among different subspecies/strains of the same oral pathogen. The findings suggest that different strains of F. nucleatum may vary greatly in terms of their pathogenicity, highlighting the importance of considering subspecies/strains in microbial pathogenicity studies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yumi Shimomura, Yutaka Sugi, Aiko Kume, Wataru Tanaka, Tsutomu Yoshihara, Tetsuya Matsuura, Yasuhiko Komiya, Yusuke Ogata, Wataru Suda, Masahira Hattori, Takuma Higurashi, Atsushi Nakajima, Mitsuharu Matsumoto
Summary: This study confirmed the identity of Fusobacterium nucleatum strains in colorectal cancer (CRC) and saliva samples using whole-genome sequencing, and developed a simple PCR method for genotyping this bacterium at the strain level. The method successfully detected identical strains in CRC samples and saliva from the same CRC patient, allowing for comprehensive identification of F. nucleatum strains in clinical samples. The findings have important implications for CRC research and clinical trials.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Bruno Perichon, Julian Lichtl-Hafele, Emma Bergsten, Vincent Delage, Patrick Trieu-Cuot, Philippe Sansonetti, Iradj Sobhani, Shaynoor Dramsi
Summary: This study indicates that the prevalence and levels of bacteria associated with colorectal cancer vary during cancer development. Only the pathobiont B. fragilis was detected in early-stage adenoma patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
HanChen Wang, Gui Xiang Yang, Yuxiang Hu, Patricia Lam, Karan Sangha, Dawn Siciliano, Anne Swenerton, Ruth Miller, Peter Tilley, Peter Von Dadelszen, Shirin Kalyan, Patrick Tang, Millan S. Patel
Summary: As metagenomic approaches have advanced, microorganisms have been found in tissues once thought to be sterile. The status of amniotic fluid remains controversial, as it is part of an immunologically privileged zone to prevent rejection of the fetus by the maternal immune system. Low nucleic acid yields and contamination from water, reagents, and the lab environment complicate attempts to address the sterile womb hypothesis.
SCIENTIFIC REPORTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Maria Muchova, Dario L. Balacco, Melissa M. Grant, Iain L. C. Chapple, Sarah A. Kuehne, Josefine Hirschfeld
Summary: This study demonstrates that biofilm formation by F. nucleatum in vitro is subspecies- and substrate-specific. Interestingly, F. nucleatum ssp. polymorphum does not appear to form stable single-subspecies continuous layers of biofilm in vitro.
FRONTIERS IN ORAL HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Yelyzaveta Razghonova, Valeriia Zymovets, Philip Wadelius, Olena Rakhimova, Lokeshwaran Manoharan, Malin Brundin, Peyman Kelk, Nelly Romani Vestman
Summary: The interaction of oral bacteria with stem cells from the apical papilla can negatively impact the success of regenerative endodontic treatment. In this study, the effects of Fusobacterium nucleatum and Enterococcus faecalis, as well as their supernatants enriched by bacterial metabolites, on the osteo- and dentinogenic potential of SCAPs were analyzed using RNA-seq transcriptomic analysis. The results showed that the bacteria and their metabolites can upregulate or downregulate the expression of genes necessary for dentinogenic and osteogenic processes, which may result in unsuccessful treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)