Review
Dentistry, Oral Surgery & Medicine
Minyan Yuan, Xueqing Zheng, Yifan Xue, Zhenru He, Guangtai Song, Yaling Song
Summary: The study aims to investigate a Chinese family with dentin dysplasia type II (DD-II) and summarize mutation hotspots, clinical manifestations, and disease management strategies. A novel pathogenic mutation (c.2035delA) in the DPP region of DSPP was identified, which was cosegregated in the family. The study revealed new clinical features of DD-II and identified a mutation hotspot in the DSPP gene.
Article
Cell Biology
Hye Jung Ihn, Ju Ang Kim, Jiwon Lim, Sang-Hyeon Nam, So Hyeon Hwang, Young Kyung Kim, Jae-Young Kim, Jung-Eun Kim, Eui-Sic Cho, Rulang Jiang, Eui Kyun Park
Summary: Tooth root development involves the interaction of growth factors and transcription factors in Hertwig's epithelial root sheath and dental mesenchyme. Knockout of the Bbx gene in mice results in reduced length of tooth roots and downregulation of Dspp expression during early root formation, suggesting a role for Bbx in tooth root formation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Zhenwei Zhang, Guibin Huang, Yu Huang, Siyi Liu, Feng Chen, Xuejun Gao, Yanmei Dong, Hua Tian
Summary: This study aimed to identify candidate genes for inheritable dentin defects in three Chinese pedigrees and characterize the property of affected teeth. Clinical and radiological features were recorded for the affected individuals. Genomic analysis identified three novel frameshift mutations of dentin sialophosphoprotein gene related to inherited dentin defects.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Mahmoud Mona, Zunnaira Abbasi, Firas Kobeissy, Abdulrahman Chahbandar, Roberta Pileggi
Summary: External root resorption (ERR) is a silent destructive phenomenon detrimental to dental health, which may have multiple etiologies. Traditional diagnostic methods like radiographs and CBCT images are not precise enough, highlighting the need for more accurate and non-invasive diagnostic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Dandrich Lim, Ko-Chien Wu, Arthur Lee, Thomas L. Saunders, Helena H. Ritchie
Summary: Dentin Sialoprotein (DSP) and phosphophoryn (PP) are two major non-collagenous proteins in dentin that play essential roles in dentin formation. Using transgenic technology, the DSPP gene knockout defect in mice can be partially or completely rescued, indicating the importance of DSPP in tooth development.
Article
Engineering, Biomedical
Luyi Zhao, Jian Sun, Ce Zhang, Chaoqun Chen, Yi Chen, Bo Zheng, Haihua Pan, Changyu Shao, Biao Jin, Ruikang Tang, Xinhua Gu
Summary: By investigating the biomimetic remineralization of demineralized dentin with Asp, it was found that Asp can promote the crystallization kinetics of PAA-stabilized ACP to HAP, shorten the remineralization time, and result in remineralized dentin with similar morphology and biological stability to natural teeth. This experiment reveals the mechanism of action of Asp in dentin biomineralization and demonstrates the potential for biomimetic remineralization of dentin with good biological properties.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Physiology
Tian Liang, Qian Xu, Hua Zhang, Suzhen Wang, Thomas G. H. Diekwisch, Chunlin Qin, Yongbo Lu
Summary: The mutant P19L-DSPP protein caused developmental enamel defects in mice, potentially linked to intracellular retention of mutant DSPP in presecretory ameloblasts.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Wei Lv, Shiyu Zhao, Yunqing Hou, Qian Tong, Yaxin Peng, Jianan Li, Zaiyan Xu, Bo Zuo
Summary: This study identified multiple SNPs in the pig lncMGPF gene that are associated with meat production traits in commercial and Chinese local pig breeds. The haplotype H1 was found to be linked to larger loin muscle area and lower backfat thickness. Further analysis showed that H1 has a longer RNA half-life and more stable secondary structure, leading to stronger effects on pig myogenic progenitor cells differentiation and muscle growth compared to haplotype H3.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Dongdong Yang, Haiying Liu, Yongping Cai, Kangyang Lu, Xiuying Zhong, Songge Xing, Wei Song, Yaping Zhang, Ling Ye, Xia Zhu, Ting Wang, Pinggen Zhang, Shi-Ting Li, Jiaqian Feng, Weidong Jia, Huafeng Zhang, Ping Gao
Summary: The catabolism of branched-chain amino acid (BCAA) is associated with tumorigenesis. This study reveals that BCAA catabolism is activated in liver cancer cells lacking glutamine. Enhanced BCAA catabolism leads to the production of BCAA-derived carbon and nitrogen for nucleotide synthesis, promoting cell-cycle progression and cell survival. The mechanism involves O-GlcNAcylation increasing under glutamine-deprivation conditions, stabilizing the PPM1K protein, and leading to dephosphorylation of BCKDHA and enhanced BCAA decomposition. Dephosphorylation of BCKDHA and high expression of PPM1K promote tumorigenesis and are associated with poor prognosis in hepatocellular carcinoma patients. Inhibiting BCAA and glutamine metabolism can slow down HCC growth in vivo. These findings shed light on the role of BCAA catabolism in tumorigenesis and identify pBCKDHA and PPM1K as potential targets for therapy and predictive biomarkers.
Article
Health Care Sciences & Services
Youn Jung Kim, Yejin Lee, Hong Zhang, Figen Seymen, Mine Koruyucu, Sule Bayrak, Nuray Tuloglu, James P. Simmer, Jan C-C Hu, Jung-Wook Kim
Summary: This study identified three novel mutations associated with hereditary dentin defects and expanded the mutational spectrum of the DSPP gene. The study also found that the expression level of the DSPP exon 3 deletion transcript correlated with the severity of dentin defects, advancing our understanding of the molecular pathogenesis of this condition.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jiapeng Ye, Yue Wang, Qinglin Zhu, Haibo Shi, Danwei Xiang, Chunyue Wu, Lina Song, Ning Ma, Qilin Liu, Wei Zhang
Summary: The study observed that transgenic full-length DSPP partially preserved the mandibular condylar morphology and MCC thickness of Dspp KO mice, while transgenic DSP failed to do so, resulting in smaller condyles and disrupted cartilage structure.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Rui Yang, Mengyao Liu, Yuqian Liu, De-Mei Meng, Tianhua Ma, Cheng-Tao Wang, Jie Liu, Qiaoe Wang, Zhongkai Zhou
Summary: Oligochitosan glycosylation can alter the structure of pea legumin and enhance its emulsification stability, thermal stability, and in vitro digestive stability. This study demonstrates the potential for functionalizing legumin through glycosylation to create protein-oligochitosan complexes for food and nutritional applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Yao Fu, Kanako Miyazaki, Yuta Chiba, Keita Funada, Tomomi Yuta, Tian Tian, Kanji Mizuta, Jumpei Kawahara, Ling Zhang, Daniel Martin, Tsutomu Iwamoto, Ichiro Takahashi, Satoshi Fukumoto, Keigo Yoshizaki
Summary: This study discovered the specific expression of GPI-APs Lypd1 in tooth development and demonstrated its important role in odontoblast differentiation. The study also revealed that Lypd1 is involved in odontoblast differentiation by regulating Smad1/5/8 phosphorylation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Elena I. Ilina, Camille Cialini, Dietlind L. Gerloff, Maitane Duarte Garcia-Escudero, Celine Jeanty, Marie-Laetitia Thezenas, Antoine Lesur, Vincent Puard, Francois Bernardin, Alina Moter, Anne Schuster, Monika Dieterle, Anna Golebiewska, Jean-Jacques Gerardy, Gunnar Dittmar, Simone P. Niclou, Tanja Mueller, Michel Mittelbronn
Summary: This study demonstrated that the expression of glycosyltransferase GLT8D1 is significantly higher in GBM patients compared to normal brain tissue, and it may be associated with decreased patient survival. In vitro experiments showed that increased GLT8D1 expression enhances GBM cell migration. Point mutation experiments revealed that the active site of GLT8D1 is essential for its glycosyltransferase activity and cell migration. Furthermore, the study identified potential interaction partners of GLT8D1 related to cytoskeleton and intracellular transport.
Article
Dentistry, Oral Surgery & Medicine
Takahiro Ikawa, Koji Mizutani, Takeaki Sudo, Chihiro Kano, Yuichi Ikeda, Tatsuya Akizuki, Hiroaki Kobayashi, Yuichi Izumi, Takanori Iwata
Summary: Electric ionic toothbrushes demonstrated significantly higher plaque removal rates than manual toothbrushes in the premolar and molar areas, while no significant difference was observed in the central incisor area.
INTERNATIONAL JOURNAL OF DENTAL HYGIENE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Koji Mizutani, Hidehiro Shioyama, Takanori Matsuura, Risako Mikami, Kohei Takeda, Yuichi Izumi, Akira Aoki, Takanori Iwata
Summary: This study found that minimally invasive periodontal surgery using EMD in patients with and without diabetes mellitus can lead to similar levels of clinical attachment gain and bone fill. This is the first documented study of successful periodontal tissue regeneration in patients with diabetes mellitus.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yuichi Ikeda, Ayako Kawada, Daiki Tanaka, Eri Ikeda, Hiroaki Kobayashi, Takanori Iwata
Summary: The study found that patients had fewer complaints with M-USSC compared to P-USSC during supragingival scaling, with significant improvements in patient complaints when using M-USSC.
INTERNATIONAL JOURNAL OF DENTAL HYGIENE
(2021)
Review
Chemistry, Physical
Shunsuke Fukuba, Munehiro Okada, Kohei Nohara, Takanori Iwata
Summary: This review provides a summary of available bone graft products worldwide and discusses the prospects of alloplastic bone substitutes in the market and trends.
Review
Biochemistry & Molecular Biology
Masahiro Hatasa, Sumiko Yoshida, Hirokazu Takahashi, Kenichi Tanaka, Yoshihito Kubotsu, Yujin Ohsugi, Takaharu Katagiri, Takanori Iwata, Sayaka Katagiri
Summary: Periodontal disease is an inflammatory disease caused by oral microorganisms, with studies showing it as a risk factor for systemic diseases. The relationship between NAFLD and periodontal disease remains unclear. Epidemiological studies suggest periodontal disease as a risk factor for NAFLD, with animal models showing acceleration of NAFLD progression by periodontopathic bacteria. Th17 may play a key role in explaining the relationship between the two diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Akira Aoki, Koji Mizutani, Risako Mikami, Yoichi Taniguchi, Yujin Ohsugi, Walter Meinzer, Yuichi Izumi, Takanori Iwata
Summary: Er:YAG laser-assisted pocket therapy can effectively and safely clean periodontal pockets, leading to significant clinical improvements in most cases without adverse side effects or complications.
CLINICAL ORAL INVESTIGATIONS
(2022)
Review
Biochemistry & Molecular Biology
Peiya Lin, Hiromi Niimi, Yujin Ohsugi, Yosuke Tsuchiya, Tsuyoshi Shimohira, Keiji Komatsu, Anhao Liu, Takahiko Shiba, Akira Aoki, Takanori Iwata, Sayaka Katagiri
Summary: Periodontitis is an inflammatory disease that involves the destruction of periodontal tissues. A new murine model using ligation has been effective in simulating bone loss and tissue destruction. The activation of osteoclasts, upregulation of pro-inflammatory factors, and excessive immune response are key factors in periodontal disruption. Multiple genes play a role in the complexity of periodontitis pathogenesis. Novel treatment methods have shown promise in the ligature-induced periodontitis model in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Yejin Lee, Hong Zhang, Figen Seymen, Youn Jung Kim, Yelda Kasimoglu, Mine Koruyucu, James P. Simmer, Jan C. -C. Hu, Jung-Wook Kim
Summary: In this study, two novel mutations in the KLK4 gene were identified in four Turkish families with hypomaturation amelogenesis imperfecta. Functional analysis revealed that these mutations resulted in the production of either an inefficient or unstable protein. This study enhances our understanding of the normal and pathological mechanisms of enamel formation.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Health Care Sciences & Services
Figen Seymen, Hong Zhang, Yelda Kasimoglu, Mine Koruyucu, James P. Simmer, Jan C. -C. Hu, Jung-Wook Kim
Summary: This study reported the findings of three families affected by amelogenesis imperfecta, identifying three novel gene mutations. The discovered mutations expand the mutational spectrum of AI-causing genes and improve our understanding of the normal and pathological amelogenesis process.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Health Care Sciences & Services
Youn Jung Kim, Yejin Lee, Hong Zhang, Figen Seymen, Mine Koruyucu, Sule Bayrak, Nuray Tuloglu, James P. Simmer, Jan C-C Hu, Jung-Wook Kim
Summary: This study identified three novel mutations associated with hereditary dentin defects and expanded the mutational spectrum of the DSPP gene. The study also found that the expression level of the DSPP exon 3 deletion transcript correlated with the severity of dentin defects, advancing our understanding of the molecular pathogenesis of this condition.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yuka Tanaka-Takemura, Yoshinori Arisaka, Masahiro Hakariya, Hiroki Masuda, Ryo Mikami, Ruriko Sekiya-Aoyama, Takanori Iwata, Tetsuya Yoda, Takashi Ono, Nobuhiko Yui
Summary: Surface properties of biomaterials, such as zeta potential and molecular mobility, play important roles in influencing macrophage responses. The zeta potential affects the initial spreading of cells, while molecular mobility governs the subsequent spreading process. Molecular mobility is the main factor modulating the inflammatory activity in macrophages.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Masahiro Hakariya, Yoshinori Arisaka, Hiroki Masuda, Tetsuya Yoda, Takanori Iwata, Nobuhiko Yui
Summary: Cancer cells respond to physical cues from the microenvironment by altering migration and chemosensitivity. The molecular mobility of polyrotaxane surfaces affects cellular spreading, migration, and chemosensitivity in lung, pancreatic, and breast cancer cells.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hiroki Masuda, Yoshinori Arisaka, Masahiro Hakariya, Takanori Iwata, Tetsuya Yoda, Nobuhiko Yui
Summary: This study evaluates the effects of molecular mobility of polyrotaxane surfaces on subcellular YAP localization and oxidative stress-induced cell senescence in human mesenchymal stem cells (HMSCs). The results suggest that the molecular mobility of polyrotaxane surfaces regulates subcellular YAP localization, thereby protecting HMSCs from oxidative stress-induced cell senescence. Applying the molecular mobility of polyrotaxane surfaces can provide insights into the prevention and treatment of diseases caused by oxidative stress.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Health Care Sciences & Services
Youn Jung Kim, Hong Zhang, Yejin Lee, Figen Seymen, Mine Koruyucu, Yelda Kasimoglu, James P. Simmer, Jan C. -C. Hu, Jung-Wook Kim
Summary: Amelogenesis imperfecta (AI) is a genetic condition characterized by various enamel defects. This study used whole exome sequencing to identify genetic mutations causing hypomaturation AI in affected families. Mutational analysis revealed WDR72 mutations as the genetic cause in four families, including novel mutations and a recurrent mutation. These findings expand our knowledge of WDR72 mutations causing hypomaturation AI and improve the accuracy of genetic testing for diagnosing AI caused by WDR72 defects.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Polymer Science
Masahiro Hakariya, Yoshinori Arisaka, Hiroki Masuda, Tetsuya Yoda, Atsushi Tamura, Takanori Iwata, Nobuhiko Yui
Summary: Researchers have designed a bilayer hydrogel structure with PRX and collagen layers to achieve rapid and strong adhesion to target tissues. In in vivo experiments in mice, the bilayer hydrogel demonstrated superior adhesion properties.