Article
Biochemistry & Molecular Biology
Koji Kitamura, Hidefumi Suzuki, Ryota Abe, Hidenori Inohara, Yasufumi Kaneda, Hidehisa Takahashi, Keisuke Nimura
Summary: This study reveals that the splicing factor SF3B2 plays a role in regulating transcription and RNA stability in head and neck squamous cell carcinoma. High SF3B2 expression is associated with poor prognosis and accelerated tumor growth. SF3B2 has a dual function in both transcription and RNA stability.
CELL AND BIOSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ioannis Galdadas, Vangelis Bonis, Paraskevi Vgenopoulou, Michail Papadourakis, Panos Kakoulidis, Georgia Stergiou, Zoe Cournia, Apostolos Klinakis
Summary: RXRs are nuclear receptors that regulate key cellular processes in all tissues. A recent study identified RXR alpha S427F mutation in bladder cancer patients, affecting the transcriptional activity of RXR alpha with varying allosteric mechanisms depending on its dimeric partner.
Article
Multidisciplinary Sciences
Samuel Rivero-Hinojosa, Elena M. Pugacheva, Sungyun Kang, Claudia Fabiola Mendez-Catala, Alexander L. Kovalchuk, Alexander Strunnikov, Dmitri Loukinov, Jeannie T. Lee, Victor V. Lobanenkov
Summary: CTCF and BORIS interact and compensate in the male germline of mice to ensure appropriate activation of spermatogenesis-specific genes, with compound mutant males being sterile due to depletion of both proteins.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Electrical & Electronic
Peng Zhou, Yanqun Liu, Hongxin Zhao, Yongrong Shi, Xiaoxing Yin
Summary: A differentially fed dual-mode patch antenna is proposed with wideband common-mode absorption, featuring a differential feeding network, a T-shaped slot for aperture coupling, and a low cross polarization 2 x 4 planar array design. The fabricated prototypes exhibit broadened -10 dB impedance bandwidths, wide CM absorption band, and low cross polarization, with measured peak realized gains of 7.5 dBi for the antenna element and 16.6 dBi for the planar array.
IEEE TRANSACTIONS ON ANTENNAS AND PROPAGATION
(2021)
Article
Biochemistry & Molecular Biology
Karina Jouravleva, Dmitrij Golovenko, Gabriel Demo, Robert C. Dutcher, Traci M. Tanaka Hall, Phillip D. Zamore, Andrei A. Korostelev
Summary: The study of the structures of Drosophila Dicer-1 reveals the collaborative mechanism of Dicer-1 and Loqs-PB in different stages, including before and after pre-miRNA binding, as well as after cleavage and product release. The findings suggest that the Dicer-1???Loqs-PB complex plays a crucial role in specific binding and cleavage of pre-miRNA, facilitating product release.
Article
Engineering, Electrical & Electronic
Zhenzhao Guo, Shengbao Wu, Jinbiao Xiao
Summary: This paper proposes a multi-order conversion scheme using asymmetric multimode interference and beam shaping, capable of converting TE0 and TE1 modes to their respective p-th and q-th modes. The dual-output channels can also be combined into a single-output channel by changing the structure.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Luiza Mamigonian Bessa, Serafima Guseva, Aldo R. Camacho-Zarco, Nicola Salvi, Damien Maurin, Laura Marino Perez, Maiia Botova, Anas Malki, Max Nanao, Malene Ringkjobing Jensen, Rob W. H. Ruigrok, Martin Blackledge
Summary: The genome replication and transcription processes of SARS-CoV-2 are important targets for inhibiting the virus. The interaction between the nucleoprotein (N) and the amino-terminal ubiquitin-like domain of nsp3 (Ubl1), which is a cofactor of the replication-transcription complex, has been described at the atomic level. This interaction involves two linear motifs in the linker domain of N, which fold N around Ubl1 to regulate binding to RNA. The identification of these motifs provides future targets for developing innovative strategies against COVID-19.
Article
Plant Sciences
Jingjing Xin, Ye Liu, Huiyun Li, Sumei Chen, Jiafu Jiang, Aiping Song, Weimin Fang, Fadi Chen
Summary: This study found that CmMLO17 in chrysanthemum plays a role in modulating plant immunity through the ABA and Ca2+ signaling pathways, reducing susceptibility to Alternaria alternata infection. Additionally, CmKIC may interact with CmMLO17 to jointly regulate chrysanthemum susceptibility to A. alternata.
HORTICULTURE RESEARCH
(2021)
Article
Engineering, Marine
Hong Yang, Xiaodie Yang, Guohui Li
Summary: A dual feature extraction and recognition system is proposed for accurately recognizing ship-radiated noise. The system utilizes pre-processing, dual feature extraction, and recognition techniques. Experimental results show that the system has good classification performance, achieving a recognition rate of up to 97.5%. The system is also successfully applied to simulated chaotic signals and measured aquatic life signals.
Article
Integrative & Complementary Medicine
Zhao Chao, Chen Min, Sun Shan-Liang, Wang Jiao-Jiao, Zhong Yue, Chen Huan-Huan, Li He-Min, Xu Han, Li Nian-Guang, Ma Hong-Yue, Wang Xiao-Long
Summary: Inspired by bufotenine and its derivatives, the quaternary ammonium salt showed analgesic effects and synergistic interactions with morphine. Reverse docking evaluation revealed close relationships with acetyl cholinesterase or nicotinic acetylcholine receptor.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Engineering, Electrical & Electronic
Zhenzhong Chen, Dongfang Guan, Zuping Qian, Wen Wu
Summary: In this paper, a dual-mode power divider based on slow-wave SIW structure is proposed for building a monopulse antenna. The antenna design approach allows for both in-phase and out-of-phase responses, offering a straightforward and feasible method for radar detection and tracking.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS II-EXPRESS BRIEFS
(2022)
Article
Biophysics
Feiyun Cui, Xiaoqun Shen, Bo Cao, Haijie Ji, Jianlei Liu, Xiwei Zhuang, Chijia Zeng, Bin Qu, Shunbo Li, Yi Xu, Qin Zhou
Summary: A biosensor integrated with mannose nano-surface was developed for the identification and adhesive strength evaluation of bacteria. S. typhimurium and E. coli JM109 (type 1 pili) were captured by the mannose nano-surface. The species of captured bacteria were identified using SERS spectra and PLS-DA. The binding affinities of the captured bacteria to the nano-surface were obtained and the results showed that S. typhimurium had a stronger adhesive strength. Normalized impedance change (NIC) was found to be positively correlated with binding affinities, indicating it can be used as an indicator for the adhesive strength of bacteria. The developed biosensor can provide both qualitative and quantitative information of selective recognition between bacteria and mannose.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Electrical & Electronic
Jing-Yu Lin, Yang Yang, Sai-Wai Wong, Yin Li
Summary: This article investigates the design and performance optimization of filtering cavity slot arrays using high-order modes, and validates the feasibility of the design methodology through the fabrication and testing of two different antenna structures.
IEEE TRANSACTIONS ON MICROWAVE THEORY AND TECHNIQUES
(2021)
Article
Biochemistry & Molecular Biology
Pamela J. E. Rowling, Ben L. Murton, Zhen Du, Laura S. Itzhaki
Summary: The interaction between beta-catenin and APC plays a crucial role in regulating the Wnt signaling pathway. APC acts as a scaffold, bringing together different components of the beta-catenin destruction complex to promote beta-catenin degradation. Mutations in APC can lead to elevated beta-catenin levels and abnormal Wnt signaling, which is associated with various types of cancer.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Physical
Vinay V. Nair, Guowei Yin, Jerry Zhang, John F. Hancock, Sharon L. Campbell, Alemayehu A. Gorfe
Summary: KRAS, as a 21 kDa guanine nucleotide-binding protein, serves as a molecular switch in regulating cellular growth. Post-translational modification by monoubiquitination at specific sites like K104 and K147 impacts the structure and function of KRAS, but the detailed mechanism is not fully understood. This study uses molecular dynamics simulations and NMR spectroscopy data to show that ubiquitination at these sites modulates conformational fluctuations in KRAS, shedding light on potential therapeutic targeting strategies.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Review
Chemistry, Multidisciplinary
Laura Burchill, Alexandra Males, Arashdeep Kaur, Gideon J. Davies, Spencer J. Williams
Summary: Endo-alpha-1,2-mannosidases and endo-alpha-1,2-mannanases are enzymes that cut within a chain and remove multiple sugar residues. Human endo-alpha-1,2-mannosidase (MANEA) can modify glucosylated mannose residues, while cell-active MANEA inhibitors can alter N-glycan processing and reduce infectivity of dengue virus. Enzymes related to Bacteroides spp. exhibit endo-alpha-1,2-mannosidase activity and can be used for inhibitor development.
ISRAEL JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Matthew G. Alteen, Hayden Peacock, Richard W. Meek, Jil A. Busmann, Sha Zhu, Gideon J. Davies, Hiroaki Suga, David J. Vocadlo
Summary: This study applied an mRNA display technology with genetic code reprogramming to identify macrocyclic peptides that can inhibit O-GlcNAc transferase (OGT) activity. These macrocycles bind to the tetratricopeptide repeats of OGT and inhibit its activity through an allosteric mechanism. The high potency and novel mechanism of action of these OGT ligands may contribute to further understanding the specificity and regulation of OGT.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Applied
Sofia F. Reis, Susana Messias, Rita Bastos, Vitor J. Martins, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Manuel A. Coimbra, Elisabete Coelho
Summary: Brewing practice involves reusing the same yeast for subsequent fermentations. The yeast used for Lager beer (Saccharomyces pastorianus) is widely reused without changes in fermentation performance. However, the yeast used for Ale beer (Saccharomyces cerevisiae) is either partially or not reused due to poor performance. The study found that cells modulate their wall polysaccharides to increase cell wall strength.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Paul H. Walton, Gideon J. Davies, Daniel E. Diaz, Joao P. Franco-Cairo
Summary: The copper histidine brace is a structural unit in metalloproteins that consists of a copper ion chelated by the NH2 and pi-N atom of an N-terminal histidine, and the tau-N atom of a further histidine in T-shaped coordination geometry. Proteins containing the histidine brace exhibit peroxygenase and/or oxygenase activity, similar to haem-containing proteins. The question of whether the functions of histidine brace-containing proteins duplicate those containing haem groups arises due to the similarities in their diversity of function.
Article
Multidisciplinary Sciences
R. Lipsh-Sokolik, O. Khersonsky, S. P. Schroder, C. de Boer, S. -Y. Hoch, G. J. Davies, H. S. Overkleeft, S. J. Fleishman
Summary: An atomistic and machine learning strategy called CADENZ was used to design enzyme fragments that combine to form diverse, low-energy structures with stable catalytic configurations. This strategy was applied to endoxylanases and resulted in the recovery of thousands of structurally diverse enzymes using activity-based protein profiling. Functional designs showed high active-site preorganization and more stable and compact packing outside the active site. Implementing these findings into CADENZ led to a 10-fold improved hit rate and over 10,000 recovered enzymes. This design-test-learn loop can be applied to any modular protein family, providing vast diversity and general principles for protein design.
Article
Food Science & Technology
Sofia F. Reis, Vitor J. Martins, Rita Bastos, Tania Lima, Viviana G. Correia, Benedita A. Pinheiro, Lisete M. Silva, Angelina S. Palma, Paula Ferreira, Manuel Vilanova, Manuel A. Coimbra, Elisabete Coelho
Summary: This study aims to investigate the feasibility of brewer's spent yeast (BSY) microcapsules as oral carriers for food and biomedical applications. The results demonstrate that BSY microcapsules have strong resistance to digestion and are rich in (beta 1 -> 3)-glucans, which can be recognized by the human Dectin-1 immune receptor. Additionally, the digested material is recognized by various lectins related to (beta 1 -> 3)-glucans, glycogen, and mannans.
Article
Agronomy
Maria P. Spinola, Monica M. Costa, Jose A. M. Prates
Summary: This study aimed to evaluate the impact of mechanical/physical pretreatments on the solubility of A. platensis proteins. The results showed that extrusion and microwave treatments significantly affected the solubility of A. platensis proteins, with extrusion causing irreversible denaturation and aggregation of major proteins, resulting in increased bioaccessibility.
Article
Agronomy
Monica M. Costa, Maria P. P. Spinola, Jose A. M. Prates
Summary: The effect of mechanical/physical pretreatments combined with commercial enzymes on the degradation of A. platensis proteins was evaluated. The results showed that extrusion combined with pancreatin was able to increase the degradation of 18-26 kDa proteins. In addition, extrusion and microwave treatment caused a decrease in total protein, possibly due to denaturation/aggregation and reduction of solubility.
Article
Biochemistry & Molecular Biology
Filipa Trovao, Viviana G. Correia, Frederico M. Lourenco, Diana O. Ribeiro, Ana Luisa Carvalho, Angelina S. Palma, Benedita A. Pinheiro
Summary: The Bacteroides thetaiotaomicron strain has developed an efficient way to degrade the complex RG-II polysaccharide using BT0996 protein and its three carbohydrate-binding modules (CBMs). The CBM at the C-terminal domain, named BT0996-C, shows unique structural features and has the ability to bind to polygalacturonic acid, one of the components of RG-II.
JOURNAL OF STRUCTURAL BIOLOGY-X
(2023)
Article
Chemistry, Applied
Claudia Afonso, Carlos Cardoso, Ana Gomes-Bispo, Ines Ferreira, Andreia Rego, Ines Coelho, Carla Motta, Jose A. M. Prates, Isabel Castanheira, Narcisa M. Bandarra
Summary: This study investigated the bioaccessibility of fatty acids, selenium, and vitamin B12 in chub mackerel and found that it is an excellent source of these essential nutrients. The consumption of chub mackerel can meet the dietary requirements for EPA+DHA, vitamin B12, and selenium.
Article
Chemistry, Physical
Salila Pengthaisong, Beatriz Piniello, Gideon J. Davies, Carme Rovira, James R. Ketudat Cairns
Summary: This study reports the structure of TxGH116 enzyme and its catalytic mechanism. The results show that the catalytic acid/base interacts with the glycosidic bond in a perpendicular manner. This has important implications for the design of specific inhibitors.
Article
Multidisciplinary Sciences
David M. Ribeiro, Rui M. A. Pinto, Paula A. Lopes, Jose M. Pestana, Cristina M. Alfaia, Monica M. Costa, Daniela F. P. Carvalho, Miguel P. Mourato, Andre M. de Almeida, Joao P. B. Freire, Jose A. M. Prates
Summary: Seaweeds, such as Laminaria digitata, are a sustainable alternative to conventional feedstuffs for weaned piglet diets, improving their health and mitigating environmental impacts. This study evaluated the impact of 10% L. digitata feed inclusion and carbohydrate-active enzymes (CAZymes) on piglet blood cells, serum metabolites, liver lipid profiles and mineral content. The results showed that L. digitata inclusion improved piglet immune response and cardiovascular health.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Laurena Silva Rodrigues, Jamile Andrea Rodrigues da Silva, Jose de Brito Lourenco-Junior, Andre Guimaraes Maciel e Silva, Andre Martinho de Almeida, Miguel Pedro Mourato, Vinicius Costa Gomes de Castro, Welligton Conceicao da Silva, Jose Antonio Mestre Prates
Summary: This study aimed to evaluate the influence of different production ecosystems (native pastures, cultivated pastures, and confinement) and seasons (dry and rainy) on the mineral content of buffalo liver in the Eastern Amazon. The results showed that the different ecosystems and seasons significantly affected the mineral content of buffalo liver, including sodium, potassium, calcium, and magnesium. Therefore, buffalo liver is a good source of minerals and the ecosystem in which the animals are raised has an impact on its content.
Review
Agriculture, Dairy & Animal Science
Maria P. Spinola, Monica M. Costa, Jose A. M. Prates
Summary: Microalgae, such as Chlorella vulgaris (CV), have been recognized as promising animal feed sources due to their high nutrient content. This study aimed to review the digestibility and accessibility of nutrients from CV biomass in monogastric animal diets. The results showed variations in nutrient digestibility when adding CV to poultry and swine diets, but pre-treatments improved accessibility. Cost-effective CV biomass can be used as a supplement or substitute for expensive feed ingredients, enhancing animal health and immunity. The differences in results may be attributed to strain variations, cultivation conditions, and dietary inclusion levels. This study provides new insights into the utilization of CV biomass in animal diets.
Article
Chemistry, Multidisciplinary
Daniel van der Gracht, Rhianna J. Rowland, Veronique Roig-Zamboni, Maria J. Ferraz, Max Louwerse, Paul P. Geurink, Johannes M. F. G. Aerts, Gerlind Sulzenbacher, Gideon J. Davies, Herman S. Overkleeft, Marta Artola
Summary: Lysosomal exoglycosidases are responsible for processing endocytosed glycans from the non-reducing end, and genetic mutations in these enzymes can cause lysosomal storage disorders. Developing effective therapeutic modalities to treat these diseases is a major objective in biomedicine. This study focuses on the development of screening assays to discover effective inhibitors for specific lysosomal glucosidases, with the aim of developing pharmacological chaperone therapeutics.