Article
Engineering, Multidisciplinary
Joshua M. Herzog, Volker Sick
Summary: Fluorescence imaging is a useful tool for characterizing tissue metabolic state and discriminating between microbial species. However, current techniques are often expensive and require specialized training. This study proposes a low-cost and robust imaging technique using ultraviolet-induced fluorescence of pyridine nucleotides and flavins in microbial samples. The technique shows high sensitivity and precision in distinguishing microbial species, making it a feasible option for rapid and low-cost microbial sample characterization.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jerzy Gebicki, Tomasz Filipiak, Andrzej Marcinek, Anna Wozniacka
Summary: Mitochondrial dysfunction is closely associated with psoriasis, and this study introduces a non-invasive method, Flow-Mediated Skin Fluorescence (FMSF), to assess mitochondrial dysfunction in psoriasis by measuring NADH autofluorescence. The results indicate an imbalance in NADH/NAD(+) redox system in psoriatic lesions compared to unaffected skin. This method not only aids in understanding the pathogenesis of psoriasis, but also provides a basis for identifying new drug targets and improving treatment.
Article
Biochemistry & Molecular Biology
Adrie H. Westphal, Dirk Tischler, Willem J. H. van Berkel
Summary: This paper highlights the natural diversity of group A FPMOs involved in the aerobic microbial catabolism of 4-hydroxybenzoate, introducing several lesser-known FAD-dependent 4-hydroxybenzoate hydroxylases and discussing their activities and structural relationships with other enzymes. The findings suggest a widespread presence of 4HB1H activity in the fungal kingdom and shed light on the phylogenetic relationships between different enzymes involved in the conversion of 4-hydroxybenzoate.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Aravinda J. De Silva, Rippa Sehgal, Jennifer Kim, John J. Bellizzi
Summary: Short-chain flavin reductases BorF and AbeF play important roles in the biosynthesis of indolotryptoline natural products by selectively chlorinating tryptophan. They demonstrate selectivity for NADH over NADPH and exhibit differences in their catalytic mechanism and substrate binding sequence. pH-rate profiles show maximum catalytic activity at pH 7.5, with certain amino acids implicated in catalytic activity and pH dependence.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Giulia Calloni, R. Martin Vabulas
Summary: Understanding the role of FAD in mammalian cryptochromes is crucial for comprehending circadian rhythms in human health and disease. While the weak binding of FAD in vitro suggests it may be functionless, the FAD-binding pocket is important for ubiquitylation and stabilization of cryptochromes. Studies have shown that increased supplies of FAD stabilize cryptochromes in cell culture and depletion of the FAD precursor affects the expression of circadian genes in mice.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Physics, Multidisciplinary
Yifan Qin, Yuanqin Xia
Summary: Two-photon fluorescence microscopy can provide physiological and pathological information from biological tissues by observing intrinsic fluorophores such as NADH and FAD. A new imaging system demonstrated in this paper allows for simultaneous capture of autofluorescence images from NADH and FAD with high excitation efficiency and minimal spectral bleed-through.
FRONTIERS IN PHYSICS
(2021)
Article
Biology
Per Niklas Hedde, Rachel Cinco, Leonel Malacrida, Andres Kamaid, Enrico Gratton
Summary: Hyperspectral imaging is widely used in various fields, and ultrafast phasor-based hyperspectral snapshot microscopy has significantly improved imaging speed and allowed for fit-free analysis of live tissues.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Physical
Kamlesh Awasthi, Tsai-En Wu, Hsin-Yun Hsu, Nobuhiro Ohta
Summary: This study investigates the effects of nanosecond pulsed electric fields (nsPEFs) on intracellular functions in lung cancer cells and normal cells using autofluorescence lifetime (AFL) microscopy of flavin adenine dinucleotide (FAD). The results show that nsPEF(50) induces apoptosis in lung cancer cells and increases the lifetime of FAD autofluorescence, while the effect on normal cells is not significant. These findings suggest that the lifetime measurements of FAD autofluorescence can be used to detect nsPEF-induced changes in intracellular functions.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Electrochemistry
Franziska Schachinger, Su Ma, Roland Ludwig
Summary: The redox potential is important for the rational use of oxidoreductases as bioelectrocatalysts. In this study, the redox potential of glucose dehydrogenase (GDH), which is widely used in glucose biosensors, was determined. The low redox potential of GDH can be used to develop glucose oxidizing bioanodes with a low operating potential, reducing susceptibility to electroactive substances in glucose biosensors or increasing the cell voltage in biofuel cells.
ELECTROCHEMISTRY COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Carlos Alberto Garcia Mogollon, Juan Carlos Quintero Diaz, Jorge Omar Gil Posada
Summary: This manuscript examines the impact of modifying the extracellular redox potential on the production of acetone, butanol, and ethanol in a microbial fuel cell. The addition of NADH enhances acetone production, while adjusting the cathode potential favors butanol production. The findings highlight the potential of bio-electrochemical systems to improve conventional fermentation processes.
BIOELECTROCHEMISTRY
(2023)
Article
Electrochemistry
Roy Cohen, Yifat Cohen, Dina Mukha, Omer Yehezkeli
Summary: Continuous glucose monitoring devices are crucial for diabetes management, requiring glucose biosensors with high accuracy and low interference. An oxygen insensitive glucose biosensor has been developed with good long term bioelectrocatalytic activity and low-cost manufacturing potential, showing promise for future sensor development.
ELECTROCHIMICA ACTA
(2021)
Article
Cell Biology
Melissa H. Broeks, Nils W. F. Meijer, Denise Westland, Marjolein Bosma, Johan Gerrits, Hannah M. German, Jolita Ciapaite, Clara D. M. van Karnebeek, Ronald J. A. Wanders, Fried J. T. Zwartkruis, Nanda M. Verhoeven-Duif, Judith J. M. Jans
Summary: The malate-aspartate shuttle (MAS) plays a crucial role in de novo serine biosynthesis, and serine supplementation could be a potential therapeutic strategy for MAS defects and other redox disorders.
Article
Chemistry, Medicinal
Zhongxian Fan, Juyang Huang, Huaiyi Huang, Samya Banerjee
Summary: Metal organometallic complexes have been introduced as catalytic cancer drugs, with the potential to disrupt important biochemical pathways in cancer cells and serve as candidates for the development of future cancer drugs.
Article
Biochemistry & Molecular Biology
Julia Messenlehner, Michael Hetman, Adrian Tripp, Silvia Wallner, Peter Macheroux, Karl Gruber, Bastian Daniel
Summary: Monolignol oxidoreductases, members of the BBE-like protein family, are FAD-dependent enzymes that exhibit a refined reaction mechanism proposed through molecular dynamics simulations for a better understanding of their catalytic mechanism.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Rhys Richard Mould, Stanley W. Botchway, James R. C. Parkinson, Elizabeth Louise Thomas, Geoffrey W. Guy, Jimmy D. Bell, Alistair V. W. Nunn
Summary: CBD, as part of the plant's defense system, has medicinal properties that impact animals' anti-cancer abilities, immune system, and epilepsy. Studies show that CBD can alter mitochondrial function and morphology, leading to oxidative stress.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
He N. Xu, Julia Tchou, Lin Z. Li
ACADEMIC RADIOLOGY
(2013)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Brian W. Pogue, David Busch, Lin Z. Li, Huiming Zhang, Dar-Bin Shieh
ACADEMIC RADIOLOGY
(2014)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nannan Sun, Weihua Luo, Lin Z. Li, Qingming Luo
ACADEMIC RADIOLOGY
(2014)
Article
Radiology, Nuclear Medicine & Medical Imaging
He N. Xu, Stephen Kadlececk, Harrilla Profka, Jerry D. Glickson, Rahim Rizi, Lin Z. Li
ACADEMIC RADIOLOGY
(2014)
Article
Biophysics
Lin Z. Li, Stephen Kadlececk, He N. Xu, Dania Daye, Benjamin Pullinger, Harrilla Profka, Lewis Chodosh, Rahim Rizi
NMR IN BIOMEDICINE
(2013)
Article
Optics
He N. Xu, Min Feng, Lily Moon, Nathan Dolloff, Wafik El-Deiry, Lin Z. Li
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2013)
Article
Optics
H. N. Xu, H. Zhao, T. A. Mir, S. C. Lee, M. Feng, R. Choe, J. D. Glickson, L. Z. Li
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2013)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yu Wen, He N. Xu, Lisa Privette Vinnedge, Min Feng, Lin Z. Li
MOLECULAR IMAGING AND BIOLOGY
(2019)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Lin Z. Li, He N. Xu, Arjun G. Yodh
MOLECULAR IMAGING AND BIOLOGY
(2019)
Article
Oncology
Junyu Cen, Lingyun Feng, Huichuan Ke, Lifeng Bao, Lin Z. Li, Yoshimasa Tanaka, Jun Weng, Li Su
Article
Biochemistry & Molecular Biology
He N. Xu, Zhenwu Lin, Chintan K. Gandhi, Shaili Amatya, Yunhua Wang, Lin Z. Li, Joanna Floros
Article
Biochemistry & Molecular Biology
He N. Xu, Joanna Floros, Lin Z. Li, Shaili Amatya
Summary: The study showed a significant impact of ozone exposure on the redox status of alveolar macrophages in mice, with freshly isolated AM being more oxidized compared to cryopreserved AM. Oxidative stress and nutrient starvation may contribute to the inability of freshly isolated AM to fully recover after being on ice for a prolonged period of time.
Article
Biochemistry & Molecular Biology
Allison Podsednik, Jinxia Jiang, Annemarie Jacob, Lin Z. Li, He N. Xu
Summary: The study evaluated the therapeutic responses of triple-negative breast cancer cells under different drug treatments using optical redox imaging, showing the effects of different drugs on cells and their synergistic interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
He N. Xu, Sarah Gourmaud, Allison Podsednik, Xiaofan Li, Huaqing Zhao, Frances E. Jensen, Delia M. Talos, Lin Z. Li
Summary: The translation above discusses a study on the decline in NAD levels in brain tissue of Alzheimer's disease models and the use of optical redox imaging to explore new biomarkers for AD. The study found that NADH, Fp, and the redox ratio were associated with AD, and suggested that ORI could be used to conveniently study the metabolism of freshly dissected brain tissues.
Article
Biochemistry & Molecular Biology
Brandon Gaitan, Collin Inglut, Udayakumar Kanniyappan, He N. Xu, Emily F. Conant, Lucas Frankle, Lin Z. Li, Yu Chen, Huang-Chiao Huang
Summary: Breast cancer is the second most deadly cancer in women, and researchers have developed an endoscopic device to differentiate between malignant tumors and normal tissue. The results show significant differences in the optical redox ratio between the core and rim of breast cancer tumors. Additionally, the FAD signal increases in the core of cancers after photodynamic therapy treatment.
